Response of St John’s wort (Hypericum empetrifolium) plants to cadmium (Cd) treatment in relation to substrate acidity/alkalinity


  • Anastasia AKOUMIANAKI-IOANNIDOU Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
  • Alexandra SALTA Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
  • Pantelis E. BAROUCHAS University of Patras, Department of Agriculture, Laboratory of Soil Science, Theodoropoulou Terma, T.K. 27200, Amaliada (GR)
  • Nicholas K. MOUSTAKAS Agricultural University of Athens, School of Environment and Agricultural Engineering, Department of Natural Resources Development and Agricultural Engineering, Laboratory of Soil Science and Agricultural Chemistry, Iera Odos 75, 118 55, Athens (GR)



Cd accumulation, heavy metals, medicinal plants, shoots, roots


The effect of cadmium (Cd) on growth and Cd accumulation in shoots and roots St John’s wort (Hypericum empetrifolium) was studied over three months in a greenhouse. Plants were cultivated in pots containing a uniform mixture of either acid or alkaline substrate consisting of peat and perlite (1:1 v/v). The pots were arranged in a completely randomized block design within two groups (acid substrate and alkaline substrate) with four Cd treatments (0-control, 1, 2, and 5 mg Cd L-1) and six replicates per treatment. Cadmium was applied as CdSO4*8/3H2O. The total amount of Cd applied per pot was 260 ml, corresponding to 0.26, 0.52, and 1.3 mg Cd per pot for doses 1, 2, and 5 mg L-1, respectively. No visual symptoms of toxicity or nutrient deficiency, as well as no differences in plant height were observed in response to Cd application, irrespective of the growth stage or substrate. There were also no differences in height development rate between the plants grown in an acidic or alkaline substrate. Cd accumulation in shoots and roots increased with increasing concentrations of applied Cd and was higher in the acidic substrate. Thus, St John’s wort plant is a Cd accumulator, especially in an acidic environment, and this in combination with its high tolerance to Cd, makes it a suitable species to remove Cd from cadmium-contaminated sites. However, for its use in the preparation of medical products, St John’s wort must be grown in a Cd-free soil so as not to pose a risk to human health. Cd extraction by (DTPA-TEA) can be employed to predict Cd accumulation in this plant.


Akoumianakis, KA, Passam HC, Barouchas PE, Moustakas NK (2008). Effect of cadmium on yield and cadmium concentration in the edible tissue of endive (Cichorium endivia L.) and rocket (Eruca sativa Mill.). Journal of Food, Agriculture and Environment 6:201-209.

Alloway BJ (1995). Cadmium. In: Alloway BJ (Ed). Heavy Metals in Soils. Blackie Academic and Professional, Glasgow pp 121-151.

Baker DE, Amacher MC (1982). Nickel, copper, zinc and cadmium. In: Page AL, Miller RH, Keeney DR (Eds). Methods of Soil Analysis. Part 2. American Society of Agronomy, Madison WI, pp 323-334.

Davis PH (1988). Flora of Turkey and the East Aegean Islands. Vol. 2. Edinburg University Press, Edinburgh, pp 396-397.

EFSA (2012). Cadmium dietary exposure in the European population. European Food Safety Authority. EFSA Journal 10(1):2551.

Genchi G, Sinicropi MS, Lauria G, Carocci A, Catalano A (2020). The effects of cadmium toxicity. International Journal of Environmental Research and Public Health 17(11):3782.

Kim KR, Owens G, Naidu R (2009). Heavy metal distribution, bioaccessibility and phytoavailability in long-term contaminated soils from Lake Macquarie. Australia. Australian Journal of Soil Research 47:166-176.

Kirkham MB (2006). Cadmium in plants on polluted soils: Effect of soil factors, hyperaccumulation and amendments. Geoderma 137:19-32.

Kitanov GM (2001). Hypericin and pseudohypericin in some Hypericum species. Biochemical Systematics and Ecology 29:171-178.

Kubier A, Wilkin RT, Pichler T (2019). Cadmium in soils and groundwater: A review. Applied Geochemistry 108:1-16.

Linsday WL, Norvell WA (1978). Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal 31:421-428.

Macarovicova E. Kralova K, Kummerova M, Kmentova E (2004). The effect of cadmium on root growth and respiration rate of two medicinal plant species. Biologia Bratislava 59(13):211-214.

Moustakas NK, Akoumianaki-Ioannidou A, Barouchas P. (2011). The effects of cadmium and zinc interactions on the concentration of cadmium and zinc in pot marigold (Calendula officinalis L.). Australian Journal of Crop Science 5:277-282.

Moustakas NK, Akoumianakis KA, Passam HC (2001). Cadmium accumulation and its effect on the yield of lettuce, radish and cucumber. Communication in Soil Science and Plant Analysis 32:1793-1802.

Petrakis P, Couladis M, Roussis V (2005). A method for detecting the biosystematic significance of the essential oil composition: The case of five Hellenic Hypericum L. species. Biochemical Systematics and Ecology 33:873-898.

Rieuwerts JS, Thornton I, Farago ME, Ashmore MR (1998). Factors influencing metal bioavailability in soils: preliminary investigations for the development of a critical loads approach for metals. Chemical Speciation & Bioavailability, 10(2):61-75.

Salta A, Akoumianaki-Ioannidou A, Barouchas PE, Moustakas NK (2019). Effects of cadmium (Cd) on dry matter and on Cd concentration in leaves and roots of purple coneflower (Echinacea purpurea L.). Bulletin UASVM Horticulture 76(1):140-142. https://doi:10.15835/buasvmcn-hort:2018.0028

STATISTICA (1998). StatSoft, Inc. Tulsa, OK, USA.

Vokou D, Katradi K, Kokkini S (1993). Ethnobotanical survey of Zagori (Epirus, Greece), a renewed center of folk medicine in the past. Journal of Ethnopharmacology 39:187-196.




How to Cite

AKOUMIANAKI-IOANNIDOU, A. ., SALTA, A. ., BAROUCHAS, P. E. ., & MOUSTAKAS, N. K. (2021). Response of St John’s wort (Hypericum empetrifolium) plants to cadmium (Cd) treatment in relation to substrate acidity/alkalinity. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12321.



Research Articles
DOI: 10.15835/nbha49212321

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