Sexual and Vegetative Propagation of Hypericum empetrifolium Willd. subsp. empetrifolium

Authors

  • Anastasia AKOUMIANAKI-IOANNIDOU Agricultural University of Athens, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, 75 Iera Odos Str., 118 55 Athens (GR)
  • Ekaterini GERASIMIDOU Agricultural University of Athens, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, 75 Iera Odos Str., 118 55 Athens (GR)
  • Alexandra SALTA Agricultural University of Athens, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, 75 Iera Odos Str., 118 55 Athens (GR)
  • Ioannis ROUSSIS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 118 55 Athens (GR)
  • Dimitrios BILALIS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 118 55 Athens (GR) https://orcid.org/0000-0002-4585-7257

DOI:

https://doi.org/10.15835/nbha47111267

Keywords:

indole-3-butyric acid (IBA); medicinal plant; ornamental value; seed germination; stem cutting

Abstract

Hypericum empetrifolium Willd. subsp. empetrifolium is an evergreen small shrub with small elongated decorative leaves and small yellow flowers in inflorescences, characterized for several pharmaceutical properties. In the present study, a first approach on the sexual and asexual propagation of this species was performed. Seeds, subjected to different types of pre-treatments [soaked in tap water; 50, 100 or 150 mg L-1 GA3 for 30 min and no treatment (control)], cultured for germination in petri dishes at 5, 10, 15, 20, 25, and 30 °C. Seed germination was only affected by temperature and the best result was obtained at 15 °C (71.2%). A significant interaction was found between pre-treatments and incubation temperature with the highest germination percentage (82%) occurred when the seeds soaked in 100 mg L-1 GA3 solution for 30 min and incubated at 20 °C constant temperature. Moreover, the germination speed was fastest from 20 to 25 °C (T50= 9.84 and 9.56 days for 20 and 25 °C, respectively). For asexual propagation, apical stem cuttings were taken at four different periods (4 seasons) and treated with IBA at concentrations of 0, 1000, 2000 and 3000 mg L-1. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Winter was the most appropriate season for cuttings collection (100% rooting percentage) and dipping in 1000 or 2000 mg L-1 IBA (72% and 73%, respectively) was the best rooting hormone treatment.

References

Akoumianaki-Ioannidou A, Martini AN, Papafotiou M (2016). Rooting and establishment of Limoniastrum monopetalum (L.) Boiss stem-tip cuttings. African Journal of Plant Science 10(1):23-31.

Balkaya A (2004). Modelling the effect of temperature on the germination speed in some legume crops. Journal of Agronomy 3(3):179-183.

Bertelle FML, Beatriz PM, Augusto LA (2004). Light, temperature and potassium nitrate in the germination of Hypericum perforatum L. and H. brasiliense Choisy seeds. Bragantia 63:193-199.

Bhardwaj DR, Mishra VK (2005). Vegetative propagation of Ulmus villosa: Effects of plant growth regulators, collection time, type of donor and position of shoot on adventitious root formation in stem cuttings. New Forest 29:105-116.

Blythe EK, Sibley JL, Tilt KM, Ruter JM (2007). Methods of auxin application in cutting propagation: A review of 70 years of scientific discovery and commercial practice. Journal of Environmental Horticulture 25:166-185.

Campbell MH (1985). Germination, emergence and seedling growth of Hypericum perforatum. Weed Research 25:259-266.

Carta A, Probert R, Puglia G, Peruzzi L, Bedini G (2016). Local climate explains degree of seed dormancy in Hypericum elodes L. (Hypericaceae). Plant Biology 18(Suppl. 1):76-82.

Chen SY, Shing-Rong K, Ching-Te C (2008). Roles of gibberellins and abscisic acid in dormancy and germination of red bayberry (Myrica rubra) seeds. Tree Physiology 28:1431-1439.

Çirac C (2007). Seed germination protocols for ex situ conservation of some Hypericum species from Turkey. American Journal of Plant Physiology 2(5):287-294.

C?irak C, Kevseroglu K, Ayan AK (2007). Breaking of seed dormancy in a Turkish endemic Hypericum species: Hypericum aviculariifolium subsp. depilatum var. depilatum by light and some pre-soaking treatments. Journal of Arid Environments 68:159-164.

C?irak C, Radusiene J, Janulis V, Ivanauskas L, Camas N, Ayan AK (2011). Phenolic constituents of Hypericum triquetrifolium Turra (Guttiferae) growing in Turkey: variation among populations and plant parts. Turkish Journal of Biology 35:449-456.

Coolbear P, Grierson D, Heydecker W (1980). Osmotic pre-sowing treatments and nucleic acid accumulation in tomato seeds (Lycopersicon lycopersicum). Seed Science and Technology 8:289-303.

Davis PH (1988). Flora of Turkey and the East Aegean Islands, vol. 2. Edinburgh University Press, Edinburgh.

Dong T, Tong J, Xiao L, Cheng H, Song S (2012). Nitrate, abscisic acid and gibberellin interactions on the thermoinhibition of lettuce seed germination. Plant Growth Regulation 66:191-202.

Diekelmann J, Schuster R (2002). Natural landscaping: Designing with native plant communities. The University of Wisconsin Press (2nd ed), Madison, WI.

Gutterman Y (2000). Maternal effects on seeds during development. In: Fenner M (Ed). Seeds: the ecology of regeneration in plant communities. Red-wood Press, Melksham, UK pp 59-84.

Hartmann HT, Kester DE, Davies FT Jr, Geneve RL (2011). Plant propagation: principles and practices. New York: Prentice-Hall (8th ed), Sao Paulo.

Kitanov GM (2001). Hypericin and pseudohypericin in some Hypericum species. Biochemical Systematics and Ecology 29:171-178.

Klein JD, Cohen S, Hebbe Y (2000). Seasonal variation in rooting ability of myrtle (Myrtus communis L.) cuttings. Scientia Horticulturae 83(1):71-76.

Leakey RRB, Chapman VR, Longman KA (1982). Physiological studies for tropical tree improvement and conservation. Some factors affecting root initiation in cuttings of Triplochiton scleroxylon K. Schum. Forest Ecology and Management 4(1):53-66.

Lu C-T, Sung J-M, Chen C-L (2008). 2,4-Dichlorophenoxyacetic acid soaking promotes rooting in stem tip cuttings of Hypericum perforatum. Journal of Agriculture and Forest 57(3-4):99-110.

Nanda KK, Kumar P, Kochhar BK (1974). Role of auxins in influencing rooting of cuttings. New Zealand Journal of Forestry Science 4:338-346.

Nordstro?m AC, Eliasson L (1991). Levels of endogenous indole-3-acetic acid and Indole-3-acetylaspartic acid during adventitious root formation in pea cuttings. Physiologia Plantarum 82(4):599-605.

Papafotiou M, Bertsouklis KF, Martini AN, Vlachou G, Akoumianaki-Ioannidou A, Kanellou E, Kartsonas ED (2017). Evaluation of the establishment of native Mediterranean plant species suggested for landscape enhancement in archaeological sites of Greece. Acta Horticulturae 1189:177-180.

Petrakis P, Couladis M, Roussis V (2005). A method for detecting the biosystematic significance of the essential oil composition: The case of five Hellenic Hypericum L. species. Biochemical Systematics and Ecology 33:873-898.

Puri S, Verma RC (1996). Vegetative propagation on Dalbergia sisoo Roxb. using softwood and hardwood stem cuttings. Journal of Arid Environments 34(2):235-245.

Salisbury FB, Ross CW (1992). Plant Physiology. Wadsworth Publishing Company (4th ed), Belmont.

Sánchez-Coronado ME, Olvera C, Márquez-Guzmán J, Macías-Rubalcava ML, Orozco S, Anaya AL, Orozco-Segovia A (2015). Complex dormancy in the seeds of Hypericum philonotis. Functional Ecology of Plants 213:32-39.

Shekhawat MS, Manokari M (2016). Impact of auxins on vegetative propagation through stem cuttings of Couroupita guianensis Aubl.: A conservation approach. Scientifica 2016, Article ID 6587571.

Thetford M, Miller D (2002). Propagation of 4 Florida coastal dune species. Native Plants Journal 3(2):112-120.

Thompson K, Jalili A, Hodgson JG, Hamzeh’ee B, Asri Y, Shaw S, … Safavi R (2001). Seed size, shape and persistence in the soil in an Iranian flora. Seed Science Research 11:345-355.

Trovato A, Raneri E, Kouladis M, Tzakou O, Taviano MF, Galati EM (2001). Anti-inflammatory and analgesic activity of Hypericum empetrifolium Willd. (Guttiferae). II Farmaco 56(5-7):455-457.

Uniyal RC, Prasad P, Nautiyal AR (1993). Vegetative propagation in Dalbergia sericea: Influence of growth hormones on rooting behaviour of stem cuttings. Journal of Tropical Forest Science 6:21-25.

Veierskov B, Andersen AS, Erikson EN (1982). Dynamics of extractable carbohydrate in Pisum sativum: I. Carbohydrate and nitrogen content in pea plants and cuttings grown at two different irradiances. Physiologia Plantarum 55:167-173.

Downloads

Published

2018-12-21

How to Cite

AKOUMIANAKI-IOANNIDOU, A., GERASIMIDOU, E., SALTA, A., ROUSSIS, I., & BILALIS, D. (2018). Sexual and Vegetative Propagation of Hypericum empetrifolium Willd. subsp. empetrifolium. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(2), 282–287. https://doi.org/10.15835/nbha47111267

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha47111267

Most read articles by the same author(s)

1 2 3 > >>