Enhancing antioxidant defense system of mung bean with a salicylic acid exogenous application to mitigate cadmium toxicity

Authors

  • Imran KHAN University of Agriculture Faisalabad, Department of Agronomy, Faisalabad, 38040 (PK)
  • Mahmoud F. SELEIMAN King Saud University, College of Food and Agriculture Sciences, Plant Production Department, P.O. Box 2460, Riyadh 11451; Menoufia University, Faculty of Agriculture, Department of Crop Sciences, Shibin El-kom 32514 (SA)
  • Muhammad U. CHATTHA University of Agriculture Faisalabad, Department of Agronomy, Faisalabad, 38040 (PK)
  • Rewaa S. JALAL University of Jeddah, College of Sciences, Department of Biology, Jeddah (SA)
  • Faisal MAHMOOD Government College University Faisalabad, Department of Environmental Sciences & Engineering, Faisalabad (PK)
  • Fahmy A. S. HASSAN Taif University, College of Science, Department of Biology, P.O. Box 11099, Taif 21944 (SA)
  • Warda IZZET University of Agriculture Faisalabad, Department of Botany, Faisalabad, 38040 (PK)
  • Bushra A. ALHAMMAD Prince Sattam Bin Abdulaziz University, College of Science and Humanity Studies, Biology Department, Al Kharj Box 292, Riyadh 11942 (SA)
  • Esmat F. ALI Taif University, College of Science, Department of Biology, P.O. Box 11099, Taif 21944 (SA)
  • Rana ROY Sylhet Agricultural University, Faculty of Agriculture, Department of Agroforestry & Environmental Science, Sylhet 3100 (BD)
  • Omer S. USLU University of Sutcu Imam, Faculty of Agriculture, Department of Field Crops (TR)
  • Muhammad U. HASSAN University of Agriculture Faisalabad, Department of Agronomy, Faisalabad, 38040 (PK)

DOI:

https://doi.org/10.15835/nbha49212303

Keywords:

antioxidant machinery, heavy metal stress, mung bean, photosynthesis, reactive oxygen species, salicylic acid

Abstract

Cadmium (Cd) accumulation is an emerging environmental hazard and has detrimental effects on plant growth and development. Salicylic acid (SA) is a well-known plant growth regulator that can initiate various molecular pathways to ameliorate Cd toxicity. The experiment was executed to scrutinize the mediatory role of SA to accelerate the defensive mechanism of mung bean in response to Cd stress. Mung bean plants were exposed to 0, 5, 10 and 15 mg Cd kg-1 of soil. Exogenous application of SA 0, 10-6 and 10-3 M was added prior flowering. Results exhibited that Cd stress considerably reduced the growth-related attributes i.e. shoot length, root length, fresh and dry biomass, total soluble protein, total amino acids, relative water contents and photosynthetic pigments. Cadmium stress showed a significant increase in antioxidants levels such as peroxidase (POD), ascorbate peroxidase (APX), ascorbic acid (AsA), and catalase (CAT) and promoted the accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents. However, exogenously applied SA significantly improved plant biomass and photosynthetic pigments under Cd stress. Moreover, SA improved the defensive system by enhancing antioxidants’ activities under the increasing concentration of Cd stress. Furthermore, SA reduced the Cd uptake, membrane damage and, H2O2 and MDA accumulation. The study's findings concluded that exogenous-applied SA enhanced plant growth, promoted the antioxidant activities, and reduced the oxidative damage in mung bean seedlings under Cd stress.

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2021-05-11

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KHAN, I. ., SELEIMAN, M. F., CHATTHA, M. U., JALAL, R. S., MAHMOOD, F. ., HASSAN, F. A. S., IZZET, W., ALHAMMAD, B. A., ALI, E. F., ROY, R., USLU, O. S., & HASSAN, M. U. (2021). Enhancing antioxidant defense system of mung bean with a salicylic acid exogenous application to mitigate cadmium toxicity. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12303. https://doi.org/10.15835/nbha49212303

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Vol. 49 No. 2 (2021)

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Research Articles
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DOI: 10.15835/nbha49212303

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Copyright (c) 2021 Imran KHAN, Mahmoud F. SELEIMAN, Muhammad U. CHATTHA, Rewaa S. JALAL, Faisal MAHMOOD, Fahmy A. S. HASSAN, Warda IZZET, Bushra A. ALHAMMAD, Esmat F. ALI, Rana ROY, Omer S. USLU, Muhammad U. HASSAN

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