Comparison of some DNA extraction methods from monovarietal must and wines

Authors

  • Anca P. ONACHE National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Adriana BĂDULESCU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Anamaria M. DUMITRU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Dorin I. SUMEDREA National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Carmen F. POPESCU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)

DOI:

https://doi.org/10.15835/nbha49212349

Keywords:

DNA quality, grapevine, spectrophotometry, SSR markers, traceability

Abstract

The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.

References

Agrimonti C, Marmiroli N (2018). PCR analysis of experimental and commercial wines by means of nuclear and chloroplast SSRs. European Food Research and Technology 244:2127-2140. https://doi.org/10.1007/s00217-018-3121-5

Akkurt M (2012). Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.). Genetics and Molecular Research 11(3):2343-2351. https://doi.org/10.4238/2012.August.13.8

Arroyo-Garcia R, Lefort F, de Andres MT, Ibanëz J, Borrego J, Jouve N, Cabello F, Martinez-Zapater JM (2002). Chloroplast microsatellite polymorphisms in Vitis species. Genome 45(6):1142-1149. https://doi.org/ 10.1139/g02–087

Baleiras-Couto MM, Eiras-Dias JE (2006). Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers. Analytica Chimica Acta 563:283-291. https://doi.org/10.1016/j.aca.2005.09.076

Bigliazzi J, Scali M, Paolucci E, Cresti M, Vignani R (2012). DNA extracted with optimized protocols can be genotyped to reconstruct the varietal composition of monovarietal wines. American Journal of Enology and Viticulture 63(4):568-573. https://www.ajevonline.org/content/63/4/568

Briciu D, Pamfil D, Briciu A, Curticiu D, Balazs E, Taoutaou A, Pop I, Cota L (2010). Development of methods for DNA extraction from leaves and must grapes. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 67(1):1843-1849. https://dx.doi.org/10.15835/buasvmcn-asb:67:1-2:5

Cabezas JA, Ibáñez J, Lijavetzky D, Vélez D, Bravo G, Rodríguez V, … Ruiz-García L (2011). A 48 SNP set for grapevine cultivar identification. BMC Plant Biology 11(153):1-12. https://doi.org/10.1186/1471-2229-11-153

Catalano V, Moreno-Sanz P, Lorenzi S, Grando MS (2016). Experimental review of DNA-based methods for wine traceability and development of a Single-Nucleotide Polymorphism (SNP) genotyping assay for quantitative varietal authentication. Journal of Agricultural and Food Chemistry 64(37):6969-6984. https://doi.org/10.1021/acs.jafc.6b02560

Drabek J, Stavek J, Jaluvkova M, Jurcek T, Frebort I (2008). Quantification of DNA during winemaking by fluorimetry and Vitis vinifera L. specific quantitative. PCR. European Food Research Technology 226:491-497. https://doi.org/10.1007/s00217-007-0561-8

Faria MA, Magalhaes R, Ferreira MA, Meredith CP, Ferreira Monteiro F (2000). Vitis vinifera must varietal authentication using microsatellite DNA analysis (SSR). Journal of Agricultural and Food Chemistry 48:1096-1100. https://doi.org/10.1021/jf990837h

Garcia-Beneytez E, Maria VM, Joaquin B, Maria CP, Javier I (2002). Application of a DNA analysis method for the cultivar identification of grape musts and experimental and commercial wines of Vitis vinifera L. using microsatellite markers. Journal of Agricultural and Food Chemistry 50(21):6090-6096. https://doi.org/10.1021/jf0202077

Gheorghe RN, Popescu CF, Pamfil D, Ciocirlan CN, Sestras R (2010). Genetic diversity of some Romanian grapevine cultivars as revealed by microsatellite markers. Romanian Biotechnological Letters 15(2):26-31. https://e-repository.org/rbl/vol.15/iss.2.s/5.pdf

Hârta MH, Pamfil D, Pop R, Vicaş S (2011). DNA fingerprinting used for testing some Romanian wine varieties. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture 68(1):143-148. https://doi.org/10.15835/buasvmcn-hort:7041

Işçi B, Yildirim H K, Altindisli A (2014). Evaluation of methods for DNA extraction. Journal of The Institute of Brewing 120:238-243. https://doi.org/10.1002/jib.129

Lazareva EG, Gilmanov KKh, Bigaeva AV, Mikhailova IYu, Semipyatny VK, Vafin RR (2020). DNA authentication technologies for product quality monitoring in the wine industry. Food Systems 3(4):11-14. https://doi.org/10/21323/2618–9771–2020–3–4–11–14

Lee A, Jain A (2017). DNA concentration measurement at 260 nm using Photopette Bio. Tip Biosystems. Application Note.1-3. AN-LS-001-DNA-Measurement. https://tipbiosystems.com/wp-content/uploads/2020/05/AN-LS-001-DNA-Measurement.pdf

Lodhi M A, Weeden N F, Reisch B I (1994). A Simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter 12(1):6-13. https://doi.org/10.1007/BF02668658

Nakamura S, Haraguchi K, Mitani N, Ohtsubo K (2007). Novel preparation method of template DNAs from wine for PCR to dif¬ferentiate grape (Vitis vinifera L.) cultivar. Journal of Agricultural and Food Chemistry 55(25):10388-10395. https://doi.org/10.1021/jf072407u

Nazhad NR, Solouki M (2008). Separation of DNA for molecular markers analysis from leaves of the Vitis vinifera. Pakistan Journal of Biological Science 11(11):1436-1442. https://scialert.net/abstract/?doi=pjbs.2008.1436.1442

Oganesyants LA, Vafin RR, Galstyan AG, Semipyatniy VK, Khurshudyan SA, Ryabova AE (2018). Prospects for DNA authentication in wine production monitoring. Foods and Raw Materials 6(2):438-448. http://doi.org/10.21603/2308-4057-2018-2-438-448

Pereira L, Guedes-Pinto H, Martins-Lopes P (2011). An enhanced method for Vitis vinifera L. DNA extraction from wines. American Journal of Enology and Viticulture 62(4):547-552. https://www.ajevonline.org/content/62/4/547

Pereira L, Gomes S, Castro C (2017). High Resolution Melting (HRM) applied to wine authenticity. Food Chemistry 216:80-86. https://doi.org/10.1016/j.foodchem.2016.07.185

Savazzini F, Martinelli L (2006). DNA analysis in wines: development of methods for enhanced extraction and real-time polymerase chain reaction quantification. Analytica Chimica Acta 563:274-282. https://doi.org/10.1016/j.aca.2005.10.078

Scali M, Elisa P, Jacopo B, Mauro C, Vignani R (2014). Vineyards genetic monitoring and Vernaccia di San Gimignano wine molecular fingerprinting. Advances in Bioscience and Biotechnology 5(2):142-154. https://doi.org/10.4236/abb.2014.52018

Siret R, Boursiquot JM, Merle MH, Cabanis JC, This P (2000). Toward the authentication of varietal wines by the analysis of grape (Vitis vinifera L.) residual DNA in must and wine using microsatellite markers. Journal of Agricultural and Food Chemistry 48(10):5035-5040. https://doi.org/10.1021/jf991168a

Siret R, Gigaud O, Rosec JP, This P. (2002) Analysis of grape Vitis vinifera L DNA in must mixtures and experimental mixed wines using microsatellite markers. Journal of Agricultural and Food Chemistry 50:3822-3827. https://doi.org//10.1021/jf011462e

Vignani R, Liò P, Scali M (2019). How to integrate wet lab and bioinformatics procedures for wine DNA admixture analysis and compositional profiling: case studies and perspectives. PLoS One 14(2):e0211962. https://doi.org/10.1371/journal.pone.0211962

Downloads

Published

2021-06-04

How to Cite

ONACHE, A. P. ., BĂDULESCU, A. ., DUMITRU, A. M., SUMEDREA, D. I. ., & POPESCU, C. F. (2021). Comparison of some DNA extraction methods from monovarietal must and wines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12349. https://doi.org/10.15835/nbha49212349

Issue

Vol. 49 No. 2 (2021)

Section

Research Articles
CITATION
DOI: 10.15835/nbha49212349

License

Copyright (c) 2021 Anca P. ONACHE, Adriana BĂDULESCU, Anamaria M. DUMITRU, Dorin I. SUMEDREA, Carmen F. POPESCU

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

License:

© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).

Open Access Journal:

The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.