Physical and chemical properties, pectinases activity, and cell wall pectin of Acidulus, Momordica, Inodorus and Cantalupensis melons with different ripening degree at harvest

  • Felipe M. PONTES University Federal Rural of Semi-Árido, Agricultural Sciences center, Francisco Mota street, 59625-900, Mossoró, RN (BR)
  • José D. A. SARMENTO University Federal Rural of Semi-Árido, Agricultural Sciences center, Francisco Mota street, 59625-900, Mossoró, RN (BR)
  • Naama J. de A. MELO University Federal Rural of Semi-Árido, Agricultural Sciences center, Francisco Mota street, 59625-900, Mossoró, RN (BR)
  • Erika V. de MEDEIROS Federal University of Agreste of Pernambuco, Bom Pastor avenue, Garanhuns, Pernambuco, 55292-270 (BR)
  • Patrícia L. D. MORAIS University Federal Rural of Semi-Árido, Agricultural Sciences center, Francisco Mota street, 59625-900, Mossoró, RN (BR)
  • Glauber H. de S. NUNES University Federal Rural of Semi-Árido, Agricultural Sciences center, Francisco Mota street, 59625-900, Mossoró, RN (BR)
Keywords: beta-galactosidase, commercial, Cucumis melo, methylesterase, non-commercial melon, polygalacturonase

Abstract

The objective of the present study was to evaluate the physical and chemical changes, pectinases activity, and cell wall pectin in melon varieties Acidulus (access 16), Momordica (access 2), Inodorus (cv. ‘Iracema’) and Cantalupensis (cv. ‘Olympic’), in the relation of ripening degree at harvest. Melon fruits were planted and evaluated with different ripening degree at harvest, from 15 to 35 days after anthesis (DAA). The fruits, arranged in a completely randomized design, had been evaluated on the harvest days to physical and chemical characteristics. We evaluate pectin methylesterase, polygalacturonase, beta-galactosidase, and pectin contents (water-soluble, chelate soluble, and sodium carbonate soluble). The ideal harvest for each melon was, 35 days after anthesis for cv ‘Iracema’, 30 days after anthesis for cv. ‘Olympic’, 30 days after anthesis for access 16, and 20 days after anthesis for access 2. High pulp firmness of access 16 is associated with the high levels of sodium carbonate soluble pectin and low levels of polygalacturonase and beta-galactosidase activity. Momordica melon fruit cracking is related to the high levels of pectinases activity, as well as pectin degradation.

Metrics

Metrics Loading ...

References

AOAC (2002). Official methods of analysis of the Association of Official Analytical Chemistry. AOAC (17th ed), Washington.

Aragao FAS, Torres J, Nunes GHS, Queiroz MA, Bordallo PN, Buso GSC, ... Neto FB (2013). Genetic divergence among accessions of melon from traditional agriculture of the Brazilian Northeast. Genetics and Molecular Research 12:6356-6371. https://doi.org/10.4238/2013.December.6.3

Araújo Neto SEd, Gurgel FdL, Pedrosa JF, Ferreira RLF, Araújo ADP (2003). Produtividade e qualidade de genótipos de melão-amarelo em quatro ambientes [Productivity and quality of genotypes of yellow melon in four environments]. Revista Brasileira de Fruticultura 25:104-107. https://doi.org/10.1590/S0100-29452003000100030

Beaulieu JC (2006). Volatile changes in cantaloupe during growth, maturation, and in stored fresh-cuts prepared from fruit harvested at various maturities. Journal of the American Society for Horticultural Science 131(1):127-139. https://doi.org/10.21273/JASHS.131.1.127

Belge B, Goulao LF, Comabella E, Graell J, Lara I (2017). Refrigerated storage and calcium dips of ripe ‘Celeste’sweet cherry fruit: Combined effects on cell wall metabolism. Scientia Horticulturae 219:182-190. https://doi.org/10.1016/j.scienta.2017.02.039

Bianchi T, Guerrero L, Gratacos-Cubarsi M, Claret A, Argyris J, Garcia-Mas J, Hortos M (2016). Textural properties of different melon (Cucumis melo L.) fruit types: Sensory and physical-chemical evaluation. Scientia Horticulturae 201:46-56. https://doi.org/10.1016/j.scienta.2016.01.028

Chen HJ, Cao SF, Fang XJ, Mu HL, Yang HL, Wang X, … Gao HY (2015). Changes in fruit firmness, cell wall composition and cell wall degrading enzymes in postharvest blueberries during storage. Scientia Horticulturae 188:44-48. https://doi.org/10.1016/j.scienta.2015.03.018

Chisari M, Silveira AC, Barbagallo RN, Spagna G, Artes F (2009). Ripening stage influenced the expression of polyphenol oxidase, peroxidase, pectin methylesterase and polygalacturonase in two melon cultivars. International Journal of Food Science & Technology 44:940-946. https://doi.org/10.1111/j.1365-2621.2008.01735.x

Chong JX, Lai SJ, Yang HS (2015). Chitosan combined with calcium chloride impacts fresh-cut honeydew melon by stabilising nanostructures of sodium-carbonate-soluble pectin. Food Control 53:195-205. https://doi.org/10.1016/j.foodcont.2014.12.035

Cohen S, Tzuri G, Harel-Beja R, Itkin M, Portnoy V, Sa'ar U, … Rogachev I (2012). Co-mapping studies of QTLs for fruit acidity and candidate genes of organic acid metabolism and proton transport in sweet melon (Cucumis melo L.). Theoretical and Applied Genetics 125:343-353. https://doi.org/10.1007/s00122-012-1837-3

Dai N, Petreikov M, Portnoy V, Katzir N, Pharr DM, Schaffer AA (2006). Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants. Plant Physiology 142:294-304. https://doi.org/10.1104/pp.106.083634

De Sousa AED, de Almeida Lopes MM, Moreira ADR, Macedo JJN, Moura CFH, de Aragão FAS, de Oliveira Silva E (2019). Induction of postharvest resistance in melon using pulsed light as abiotic stressor. Scientia Horticulturae 246: 921-927. https://doi.org/10.1016/j.scienta.2018.11.066

Dhillon NPS, Ranjana R, Singh K, Eduardo I, Monforte AJ, Pitrat M, … Singh PP (2007). Diversity among landraces of Indian snapmelon (Cucumis melo var. momordica). Genetic Resources and Crop Evolution 54:1267-1283. https://doi.org/10.1007/s10722-006-9108-2

Esteras C, Rambla JL, Sánchez G, Granell A, Picó MB (2020). Melon genetic resources characterization for rind volatile profile. Agronomy 10(10):1512. https://doi.org/10.3390/agronomy10101512

Ionica ME, Nour V, Trandafir I (2015). Evolution of some physical and chemical characteristics during growth and development of muskmelon (Cucumis melo L.). Pakistan Journal of Agricultural Sciences 52:265-271.

Jen JJ, Robinson ML (1984). Pectolytic enzymes in sweet bell peppers (Capsicum annuum). Journal of Food Science 49:1085-1087. https://doi.org/10.1111/j.1365-2621.1984.tb10398.x

Kozioł A, Cybulska J, Pieczywek PM, Zdunek A (2017). Changes of pectin nanostructure and cell wall stiffness induced in vitro by pectinase. Carbohydrate Polymers 161:197-207. https://doi.org/10.1016/j.carbpol.2017.01.014

Li M, Han D, Liu W (2019). Non-destructive measurement of soluble solids content of three melon cultivars using portable visible/near infrared spectroscopy. Biosystems Engineering 188:31-39. https://doi.org/10.1016/j.biosystemseng.2019.10.003

Li X, Li C, Sun J, Jackson A (2020). Dynamic changes of enzymes involved in sugar and organic acid level modification during blueberry fruit maturation. Food Chemistry 309:125617. https://doi.org/10.1016/j.foodchem.2019.125617

Li XW, Cao SF, Zheng YH, Sun AP (2011). 1-MCP suppresses ethylene biosynthesis and delays softening of 'Hami' melon during storage at ambient temperature. Journal of the Science of Food and Agriculture 91:2684-2688. https://doi.org/10.1002/jsfa.4514

Liu B, Jiao W, Wang B, Shen J, Zhao H, Jiang W (2019). Near freezing point storage compared with conventional low temperature storage on apricot fruit flavor quality (volatile, sugar, organic acid) promotion during storage and related shelf life. Scientia Horticulturae 249:100-109. https://doi.org/10.1016/j.scienta.2019.01.048

Manohar SH, Murthy HN (2012). Estimation of phenotypic divergence in a collection of Cucumis melo, including shelf-life of fruit. Scientia Horticulturae 148:74-82. https://doi.org/10.1016/j.scienta.2012.09.025

Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry 31:426-434. https://doi.org/10.1021/ac60147a030

Munira ZA, Rosnah S, Zaulia O, Russly AR (2013). Effect of postharvest storage of whole fruit on physico-chemical and microbial changes of fresh-cut cantaloupe (Cucumis melo L. reticulatus cv. Glamour). Food Research International 1:501-508.

Ortiz-Duarte G, Pérez-Cabrera LE, Artés-Hernández F, Martínez-Hernández GB (2019). Ag-chitosan nanocomposites in edible coatings affect the quality of fresh-cut melon. Postharvest Biology and Technology 147: 174-184. https://doi.org/10.1016/j.postharvbio.2018.09.021

Pressey R, Avants JK (1973). Two forms of polygalacturonase in tomatoes. Biochimica et Biophysica Acta 309:363-369. https://doi.org/10.1016/0005-2744(73)90035-1

Purquerio LFV, Cecílio Filho AB (2005). Nitrogen concentration in nutrient solution and number of fruits on quality of melon fruits. Horticultura Brasileira 23:831-836. https://doi.org/10.1590/S0102-05362005000300028

Queiroga RCFD, Puiatti M, Fontes PCR, Cecon PR (2008). Produtividade e qualidade do melão Cantaloupe, cultivado em ambiente protegido, variando número e a posução dos frutos na planta. Bragantina 67:911-920. https://doi.org/10.1590/S0006-87052008000400013

Rajulapati V, Goyal A (2017). Molecular cloning, expression, and characterization of pectin methylesterase (CtPME) from Clostridium thermocellum. Molecular Biotechnology 59(4-5):128-140. https://doi.org/10.1007/s12033-017-9997-7PMID: 28332030

Rizzo AA, Braz LT (2001). Características de cultivares de melão rendilhado cultivafas em casa de vegetação [Evaluation of qualitative characteristics of netted melon fruits under greenhouse conditions]. Horticultura Brasileira 19:370-373. https://doi.org/10.1590/S0102-05362001000300017

Sales R, Nunes GHS, Silva KJP, Costa GG, Guimaraes IM, Michereff SJ (2015). Reaction of melon accessions to Rhizoctonia solani. Horticultura Brasileira 33:196-202. https://doi.org/10.1590/S0102-053620150000200010

Silveira AC, Aguayo E, Chisari M, Artes F (2011). Calcium salts and heat treatment for quality retention of fresh-cut 'Galia' melon. Postharvest Biology and Technology 62:77-84. https://doi.org/10.1016/j.postharvbio.2011.04.009

Strohecker R, Henining HM (1967). Análisis de vitaminas: métodos comprobrados. Madrid, Paz Montalvo.

Supapvanich S, Tucker GA (2013). The effect of 1-methylcyclopropene (1-MCP) on quality and cell wall hydrolases activities of fresh-cut muskmelon (Cucumis melo var reticulatus L.) during storage. Food Biotechnology 6:2196-2201. https://doi.org/10.1007/s11947-011-0776-3

Tadmor Y, Burger J, Yaakov I, Feder A, Libhaber SE, Portnoy V, Meir A, Tzuri G, Saar U, Rogachev I (2010). Genetics of flavonoid, carotenoid, and chlorophyll pigments in melon fruit rinds. Journal of Agricultural and Food Chemistry 58:10722-10728. https://doi.org/10.1021/jf1021797

Taiz L, Zeiger E (2010). Plant physiology. Sinauer Associates.

Tomaz HVD, Aroucha EMM, Nunes GHD, Neto FB, Queiroz RF (2009). Postharvest quality of different yellow melon hybrids stored under refrigeration. Revista Brasileira de Fruticultura 31:987-994. https://doi.org/10.1590/S0100-29452009000400011

Vallone S, Sivertsen H, Anthon GE, Barrett DM, Mitcham EJ, Ebeler SE, Zakharov F (2013). An integrated approach for flavour quality evaluation in muskmelon (Cucumis melo L. reticulatus group) during ripening. Food Chemistry 139:171-183. https://doi.org/10.1016/j.foodchem.2012.12.042

Zhang H, Wang HS, Yi HP, Zhai WQ, Wang GZ, Fu QS (2016). Transcriptome profiling of Cucumis melo fruit development and ripening. Horticulture Research 3:10. https://doi.org/10.1038/hortres.2016.14

Published
2021-04-29
How to Cite
PONTES, F. M., SARMENTO, J. D. A., de A. MELO, N. J., de MEDEIROS, E. V., MORAIS, P. L. D., & de S. NUNES, G. H. (2021). Physical and chemical properties, pectinases activity, and cell wall pectin of Acidulus, Momordica, Inodorus and Cantalupensis melons with different ripening degree at harvest. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12062. https://doi.org/10.15835/nbha49212062
Section
Research Articles
CITATION
DOI: 10.15835/nbha49212062