Relationship between some morphological traits of the tomato lines and resistance to tomato yellow leaf curl virus disease

  • Sinan ZENGIN Antalya Agriculture Production Consultancy and Marketing Company, Antalya
  • Aylin KABAS Akdeniz University, Manavgat Vocational School, Antalya
  • Hulya ILBI Ege University, Faculty of Agriculture, Department of Horticulture, Izmir
Keywords: biological test; mas; morphological traits; tomato; ty-3a, resistance


Tomato (Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. The most efficient and environmentally friendly method against TYLCV is the use of resistant varieties. In this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptible inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F1 and F2 generations and biological tests were done for TYLCV resistance in F3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests. In total of 95 genotypes in F3 were developed with molecular marker selection. It was determined that 30 genotypes having the Ty-3a and Ty-1 genes as homozygous resistant. The nine genotypes carried these genes in heterozygous form. 56 genotypes were identified as susceptible. The 43 morphological traits were observed in identified individuals to correlate with resistant allele, Ty-3a. It was found that there was statistically important correlation between Ty-3a and length of internode, length of stem at first inflorescence, status of calix, leaf attitude, length of inflorescence and plant habitus. Also, there was negative correlation between fruit weight, fruit length and resistance. Therefore, we identified some morphological markers linked to Ty-3a which can be used in selection for TLYCV resistant breeding programme.


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Balliu A, Hallidri M (2002). Combining ability test between some tomato genotypes. Acta Horticulturae 579:123-126.

Chen FQ, Foolad MR, Hyman J, Clair DA, Beeleman RB (1999). Mapping QTLs for lycopene and other fruit traits in a Lycopersicon esculentum x L. pimpinellifolium cross and comparison of QTLs across tomato species. Molecular Breeding 5:283-299.

Cho JJ, Custer DM, Brommonschenkel SH, Tanksley SD (1995). Conventional breeding: host-plant resistance and the use of molecular markers to develop resistance to tomato spot wilt virus in vegetables. Tospoviruses and Thrips of Floral and Vegetable Crops 431:367-378.

Consuegra OG, Gómez MP, Zubiaur YM (2015). Pyramiding TYLCV and TSWV resistance genes in tomato genotypes. Revista de Protección Vegetal 30(2):161-164.

Czosnek H, Laterrot H (1997). A worldwide survey of tomato yellow leaf curl viruses. Archives of Virology 142:1391-1406.

De Castro AP, Blanca JM, Díez MJ, Viñals FN (2007). Identification of a CAPS marker tightly linked to the tomato yellow leaf curl disease resistance gene Ty-1 in tomato. European Journal of Plant Pathology 117:347-356.

Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002). A comparative genetic linkage map of eggplant (Solanum melongena) and its implications for genome evolution in the Solanaceae. Genetics 161(4):1697-1711.

Doyle JJ, Doyle J (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical Bulletin 19:11-15.

FAO (2017). Statistics of food and agriculture organization of the united nations.

Foolad MR (2007). Genome mapping and molecular breeding of tomato. International journal of plant genomics.

Ghanim M, Czosnek H (2000). Tomato yellow leaf curl gemini virus (TYLCV-Is) is transmitted among whiteflies (Bemisia tabaci) in a sex-related manner. Journal of Virology 74:4738-4745.

Gómez O, Piñón M, Martínez Y, Quiñones M, Fonseca D, Laterrot H (2004). Breeding for resistance to begomovirus in tropic-adapted tomato genotypes. Plant Breeding 123:275-279.

Grandillo S, Ku HM, Tanksley SD (1999). Identifying the loci responsible for natural variation in fruit size and shape in tomato. Theoretical and Applied Genetics 99(6):978-987.

Hanson P, Lu SF, Wang JF, Chen W, Kenyon L, Tan CW, … Ledesma D (2016). Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Scientia Horticulturae 201:346-354.

Hassan AA, Wafi MS, Quronfilah NE, Obaji UA, Al-Rayis MA, Al-Izabi F (1991). Screening for tomato yellow leaf curl virus resistance in wild and domestic Lycopersicon accessions. Tomato Genetics Cooperative 41:19-21.

Jensen KS, Van Betteray B, Smeets J, Yuanfu J, Scott JW, Mejia L, … Maxwell DP (2007). Co dominant SCAR Marker, P6-25, for Detection of the ty-3, Ty-3, and Ty-3a alleles at 25 cM of chromosome 6 of tomato.

Ji Y, Schuster DJ, Scott JW (2007). Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato. Molecular Breeding 20(3):271-284.

Mejía L, Teni RE, Vidavski F, Czosnek H, Lapidot M, Nakhla MK, Maxwell DP (2005). Evaluation of tomato germplasm and selection of breeding lines for resistance to begomoviruses in Guatemala. Acta Horticulturae 695:251-255.

Mutlu N, Demirelli A, Ilbi H, Ikten C (2015). Development of co-dominant SCAR markers linked to resistant gene against the Fusarium oxysporum f. sp. radicis-lycopersici. Theoretical and Applied Genetics 128(9):1791-1798.

Oerke EC (2006). Crop losses to pests. The Journal of Agricultural Science 144(1):31-43.

Padidam M, Beachy RN, Fauquet CM (1995). Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76(1):25-35.

Picó B, Díez MJ, Nuez F (1996). Viral diseases causing the greatest economic losses to the tomato crop. II. The tomato yellow leaf curl virus-a review. Scientia Horticulturae 67(3-4):151-196.

Polston JE, Anderson PK (1997). The emergence of whitefly-transmitted geminiviruses in tomato in the western hemisphere. Plant Disease 81(12):1358-1369.

Rani IC, Veeraragavathatham D, Sanjutha S (2008). Studies on correlation and path coefficient analysis on yield attributes in root knot nematode resistant F1 hybrids of tomato. Journal of Applied Sciences Research 4(3):287-295.

Scott JW, Schuster DJ (1991). Screening of accessions for resistance to the Florida tomato geminivirus. Tomato Genetics Cooperative 41:48-50.

Scott JM (2007). Breeding for resistance to viral pathogens. Genetic Improvement of Solanaceous Crops 2:457-485.

Tanksley SD (2004). The genetic developmental and molecular bases of fruit size and shape variation in tomato. The Plant Cell 16:181-189.

Verlaan MG, Hutton SF, Ibrahem RM, Kormelink R, Visser RG, Scott JW, … Bai Y (2013). The tomato yellow leaf curl virus resistance genes Ty-1 and Ty-3 are allelic and code for DFDGD-class RNA-dependent RNA polymerases. PLoS Genetics 9(3):e100339.

Zamir D, Ekstein-Michelson I, Zakay Y (1994). Mapping and introgression of a tomato yellow leaf curl virus tolerance gene, Ty-1. Theoretical and Applied Genetics 88 (2):141-146.

Zeidan M, Czosnek H (1991). Acquisition of tomato yellow leaf curl virus by the whitefly Bemisia tabaci. Journal of General Virology 72(11):2607-2614.

How to Cite
ZENGIN, S., KABAS, A., & ILBI, H. (2020). Relationship between some morphological traits of the tomato lines and resistance to tomato yellow leaf curl virus disease. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 388-397.
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