Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha <p style="text-align: justify;"><strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> is a peer-reviewed&nbsp;<strong>quarterly (beginning in 2019)</strong> journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (<strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> or JCR abbrev. title <strong>Not Bot Horti Agrobo</strong>) are mentioned.</p> <p style="text-align: justify;"><strong>Editor-in-Chief</strong></p> <ul> <li class="show"><a href="https://www.sestras.ro/">Radu E. SESTRAS</a></li> </ul> <p><strong>Advisory Editors</strong></p> <ul> <li class="show">Cornel CATOI</li> <li class="show">Viorel MITRE</li> <li class="show">Doru C. PAMFIL</li> </ul> <p><a href="/index.php/nbha/about/editorialTeam" target="_blank" rel="noopener"><strong>Editorial Board</strong></a></p> <p><strong>Journal Secretary</strong></p> <ul> <li class="show">Roxana L. STOIAN</li> <li class="show">Silviu L. MIC</li> </ul> <p>&nbsp;</p> <p>Print <strong>ISSN 0255-965X; </strong>Electronic <strong>ISSN 1842-4309</strong></p> <p><strong>License: &nbsp;</strong><strong> <a title="CC BY 4.0" href="https://creativecommons.org/licenses/by/4.0/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/by1.png" width="80" height="28"></a></strong><strong>&nbsp; <a title="About The Licenses" href="https://creativecommons.org/licenses/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/cc.logo_.png" width="118" height="28"></a></strong></p> <p>&nbsp;</p> <p><strong>Open Access Journal:</strong> Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.</p> <p>&nbsp;</p> <ul> <li class="show">See information on <strong>‘</strong><strong>Policies’</strong> (<a href="/index.php/nbha/focus-scope">Focus and Scope</a>; <a href="/index.php/nbha/about/editorialPolicies#peerReviewProcess">Peer Review Process</a>; <a href="/index.php/nbha/about/editorialPolicies#publicationFrequency">Publication Frequency</a>; <a href="/index.php/nbha/about/editorialPolicies#openAccessPolicy">Open Access Policy</a>; <a href="/index.php/nbha/about/editorialPolicies#custom-0">Publication Ethics and Publication Malpractice Policy</a> etc.); <strong>‘</strong><strong>Submissions’</strong> (<a href="/index.php/nbha/about/submissions#onlineSubmissions">Online Submissions</a>; <a href="/index.php/nbha/about/submissions#authorGuidelines">Author Guidelines</a>; <a href="/index.php/nbha/about/submissions#copyrightNotice">Copyright Notice</a>; <a href="/index.php/nbha/about/submissions#privacyStatement">Privacy Statement</a>; <a href="/index.php/nbha/about/submissions#authorFees">Payment</a>&nbsp;etc.).</li> </ul> <p>&nbsp;</p> <ul> <li class="show"><strong>June 1, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in <strong><span style="color: #297439;"><a class="cta-secondary" style="color: #297439;" title="CiteScore 2018 value" href="https://journalmetrics.scopus.com/" target="_blank" rel="noopener noreferrer">CiteScore rank (Scopus – Elsevier)</a></span></strong><strong> <span style="color: #297439;"><a href="https://www.scopus.com/sourceid/17700156420?origin=sbrowse">#34/72 in Horticulture</a> </span></strong></li> <li class="show"><strong>June 26, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in<strong> <span style="color: #297439;"><a href="https://clarivate.com/blog/science-research-connect/the-2018-jcr-release-is-here/"><span class="cta-secondary" style="color: #297439;">ISI Clarivate Analytics</span> 183/222 in Plant Sciences</a></span></strong><strong><span style="color: #297439;">&nbsp;</span></strong></li> </ul> <p align="center">&nbsp;</p> <p align="center"><strong>Published by AcademicPres on behalf of:</strong></p> <p align="center">University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca</p> <p align="center">Romanian Academy of Agricultural and Forestry Sciences</p> <p align="center">Horticulture and Forestry Society from Transylvania</p> <p align="center">&nbsp;</p> <p class="functie"><strong>Abstracted and/or indexed:</strong></p> <ul> <li class="show">Clarivate Analytics, Master Journal List - SCIE, JCR, <a href="http://mjl.clarivate.com/">http://mjl.clarivate.com/</a>&nbsp;(IF 2017=0.648)</li> <li class="show">Scopus - Elsevier, <a href="https://www.scopus.com/sourceid/17700156420">https://www.scopus.com/sourceid/17700156420</a> (CiteScore 2017, metrics 2018=0.78)</li> <li class="show">ProQuest,&nbsp;<a href="http://www.proquest.com/">http://www.proquest.com/</a></li> <li class="show">Centre for Agriculture and Biosciences International (CABI),&nbsp;<a href="https://www.cabdirect.org/">https://www.cabdirect.org/</a></li> <li class="show">Chemical Abstracts Service (CAS),&nbsp;<a href="https://www.cas.org/">https://www.cas.org/</a></li> <li class="show">Russian Scientific and Technical Information Institute (VINITI),&nbsp;<a href="http://www.viniti.ru/">http://www.viniti.ru</a></li> <li class="show">Bielefeld Academic Search Engine (BASE),&nbsp;<a href="http://www.base-search.net/">http://www.base-search.net</a></li> <li class="show">Google Scholar,&nbsp;<a href="http://scholar.google.com/">http://scholar.google.com</a></li> <li class="show">British Library Direct (BLD),&nbsp;<a href="http://explore.bl.uk/">http://explore.bl.uk/</a></li> <li class="show">Mendeley,&nbsp;<a href="https://www.mendeley.com/research-papers/?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">https://www.mendeley.com</a></li> <li class="show">The European Library,&nbsp;<a href="http://www.theeuropeanlibrary.org/tel4/search?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">http://www.theeuropeanlibrary.org</a></li> <li class="show">Harvard Library,&nbsp;<a href="http://hollis.harvard.edu/primo_library/libweb/action/display.do?tabs=detailsTab&amp;ct=display&amp;fn=search&amp;doc=HVD_ALEPH011313468&amp;indx=1&amp;recIds=HVD_ALEPH011313468&amp;recIdxs=0&amp;elementId=0&amp;renderMode=poppedOut&amp;displayMode=full&amp;frbrVersion=&amp;frbg=&amp;&amp;vl(51615747UI0)=title&amp;dscnt=0&amp;vl(1UIStartWith0)=contains&amp;scp.scps=scope%3A%28HVD_FGDC%29%2Cscope%3A%28HVD%29%2Cscope%3A%28HVD_VIA%29&amp;tb=t&amp;mode=Basic&amp;vid=HVD&amp;tab=books&amp;srt=rank&amp;vl(394521272UI1)=all_items&amp;vl(freeText0)=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;dum=true&amp;dstmp=1510572056817">http://hollis.harvard.edu</a></li> <li class="show">Tianjin University of Finance and Economics (TJUFE),&nbsp;<a href="http://en.tjufe.findplus.cn/?h=search_list&amp;query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;action[addexpander][]=fulltext">http://en.tjufe.findplus.cn</a></li> <li class="show">Open Archives Initiative Protocol for Metadata Harvesting (OAIster),&nbsp;<a href="http://www.oaister.org/">http://www.oaister.org</a></li> <li class="show">China Educational Publications Import and Export Corporation (SOCOLAR),&nbsp;<a href="http://www.socolar.com/">http://www.socolar.com</a></li> <li class="show">Ulrich's Periodicals Directory,&nbsp;<a href="http://www.ulrichsweb.com/">http://www.ulrichsweb.com</a></li> <li class="show">Elton B Stephens COmpany (EBSCO),&nbsp;<a href="http://www.ebscohost.com/">http://www.ebscohost.com</a></li> <li class="show">Information Matrix for the Analysis of Journals (MIAR),&nbsp;<a href="http://miar.ub.edu/">http://miar.ub.edu/</a></li> </ul> <div>&nbsp;</div> <p align="center"><em>First published 1965</em></p> <p align="center"><em>Print ISSN 0255-965X, Electronic ISSN 1842-4309</em></p> <p class="subpunct" align="center"><strong><em><a href="/index.php/nbha">www.notulaebotanicae.ro</a></em></strong></p> University of Agricultural Sciences and Veterinary Medicine (UASVM), Cluj-Napoca, Romania en-US Notulae Botanicae Horti Agrobotanici Cluj-Napoca 0255-965X <p style="text-align: justify;"><strong>License:</strong></p> <div style="text-align: justify;">© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal <strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca </strong>are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).</div> <div> <p style="text-align: justify;"><strong><img src="/public/site/images/silviu_mic/cc.logo_.large_1.png"></strong></p> <p style="text-align: justify;"><strong>Open Access Journal:</strong></p> <p style="text-align: justify;">The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.</p> <p style="text-align: justify;"><img src="/public/site/images/silviu_mic/open-access-vector-logo.png"></p> </div> Ecology, Cultivation and Utilization of the Aromatic Greek Oregano (Origanum vulgare L.): A Review https://www.notulaebotanicae.ro/index.php/nbha/article/view/11296 <p style="text-align: justify;">Medicinal and aromatic plants represent a stable part of the natural biodiversity legacy of many countries in the world. The present review focuses on oregano (<em>Origanum vulgare</em> L.; family Lamiaceae), an endemic herb in Greece that constitutes one of the best known aromatic and medicinal plants originating in the Mediterranean region. In particular, oregano is an evergreen, rich in natural compounds perennial plant that received increased attention in the last years for a wide range of uses. Oregano dry leaves and inflorescences in mixture are used as human and animal food that is extremely rich in antioxidative properties. Additionally, its essential oil is rich in carvacrol, thymol, c-terpinene and p-cymene, and is used for a number of medicinal purposes, e.g. for inhibiting microbial and fungal toxin production as well as for the well-known anti-inflammatory, analgesic, antiarthritic, antiallergic, anticarcinogenic, antidiabetic, cardioprotective, gastroprotective, hepatoprotective, and neuroprotective properties. Due to its perfect quality and high essential oil concentration, Greek oregano (<em>O. vulgare</em> ssp. <em>hirtum</em>) is regarded among the best in the world gaining in popularity in the global markets for food industry applications. Consequently, oregano might be considered as an important low-input, environmental friendly commodity for extensive cultivation in Greece. The present review summarizes on the origin, the morphology, the ecology and the utilization of this plant. Despite the extensive literature available on the use of oregano biomass and essential oil, only few reports exist concerning the cultivation of this plant. Therefore, the present review is additionally focused on the cultivation practices and the importance of cultivation and utilization of <em>Origanum vulgare</em> L. in Greece and generally in the Mediterranean region in the near future, as it constitutes a plant species with high medical, economic and environmental value.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Elpiniki SKOUFOGIANNI Alexandra D. SOLOMOU Nicholaos G. DANALATOS Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311296 Seed Treatment Techniques to Improve Germination of Wild Asparagus (Asparagus acutifolius L.), a Potential New Crop https://www.notulaebotanicae.ro/index.php/nbha/article/view/11554 <p style="text-align: justify;">Pre-sowing seed treatment techniques of stratification and scarification were used in order to find the most appropriate method to overcome dormancy of wild asparagus (<em>Asparagus acutifolius </em>L.) seeds. An indoor and an outdoor experiment were carried out at the Agricultural University of Athens. For the indoor experiment, the pre-sowing treatments of stratification (fresh and stratified seeds) and scarification (non-scarified, mechanical scarification and chemical scarification with sulfuric acid) were used as the main treatments, and three different temperatures (15, 20 and 25 °C) as sub-treatments. For the outdoor experiment, there were only the treatments of stratification and scarification. The stratification of asparagus seeds was found to have a positive effect on germination and vigor indices compared to non-stratified (fresh) seeds. The highest germination percentage, vigor index I and II were recorded with the stratified seeds that had been mechanically scarified in both indoor and outdoor conditions. The results indicate that just the scarification cannot improve germination of fresh collected seeds of wild asparagus. Stratification increased the germination percentage from 2.7-6.6% to 45.1-75.3%. The mechanical and the chemical scarification had a positive effect on the root length compared to the non-scarified seeds that have been stratified. These pre-sowing treatments can increase the germination percentage and produce vigorous seedlings that can be used to establish plantations of this potential new crop.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Nikolaos KATSENIOS Ioannis E. ROUSSIS Aspasia EFTHIMIADOU Ioanna KAKABOUKI Dimitrios BILALIS Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-16 2019-07-16 47 3 10.15835/nbha47311554 A New Measurement Model to Estimate the Intensity of Acrotony on the Latent Buds of Grapevine Canes (Vitis vinifera L.) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11452 <p style="text-align: justify;">In warm regions, such as in southern Greece, the climate change can lead to prolonged dormancy as well as to problems in bud dormancy (delay in breaking time, reduce the rate of budbreak, intensity of the phenomenon of acrotony, grapevine bud fall, disorders in bloom with intense blossom dropping, etc.), with a significant impact on the production of the vines. In these areas, it is necessary to apply chemical substances in order to break the dormancy and advance budbreak, especially when it comes to new table grape varieties, most of which are seedless. Another phenomenon which is observed on grapevines and is directly associated with budbreak is acrotony, where the apical buds of the cane break first compared to the middle and basal ones. Acrotony can constitute a problem because it can cause irregular grape ripening, different timing of various activities in the vineyard, which brings about higher cultivation costs. In this research, a new method to measure and evaluate the acrotony and its intensity on the latent buds of grapevine canes was described. The results of two chemical substances applied on table grape variety ‘Prime’ in order to advance budbreak were presented.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Ioannis DASKALAKIS Katerina BINIARI Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-10 2019-07-10 47 3 10.15835/nbha47311452 Some Biochemical Characteristics of Fruit Samples of Sambucus nigra Genotypes from Northeastern Turkey https://www.notulaebotanicae.ro/index.php/nbha/article/view/11489 <p style="text-align: justify;">There are numerous wild edible fruit species in the world and they are important components of dietary utilization, local pharmacopoeia, cultural and religious practices of indigenous people. In this study, some important berry characteristics of seven seed propagated black elderberry (<em>Sambucus nigra)</em> genotypes sampled from Rize province, Turkey, were assessed. The genotypes exhibited a great diversity on most of the biochemical characteristics. Berries of genotypes showed moderate Vitamin C content ranged from 26 to 38 mg per 100 g fresh weight. Total phenolic and total anthocyanin content in fruits of the <em>S. nigra </em>genotypes has also high value ranged from 360 to 447 mg gallic acid equivalent (GAE) per 100 g fresh weight and 307 to 412 mg cyanidin-3-glucoside equivalent per 100 g fresh berry, respectively. Antioxidant activity was found between 5.28 and 7.11 mmol trolox equivalent per 100 g fresh weight basis. Citric acid and fructose determined as major organic acid and sugar for all genotypes. For its high vitamin C, total anthocyanin and total phenolic content and high antioxidant activity of fruits of wild grown <em>S. nigra</em> may have provide many health benefits and can be domesticated to establish orchards in Turkey.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Tuncay KAN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-04-10 2019-04-10 47 3 10.15835/nbha47311489 Influence of Selected Antibiotics on the Tomato Regeneration in In Vitro Cultures https://www.notulaebotanicae.ro/index.php/nbha/article/view/11401 <p style="text-align: justify;">Generally, antimicrobial agents are frequently used in micropropagation techniques to obtain free elite clones or after genetic transformation to select putative transformants. Their successful application minimizes bacterial contamination however; they may be phytotoxic and may diversely affect the regeneration ability in plant tissue cultures. The objective of the current study was to estimate the effects of four antibiotics i.e. ampicillin, carbenicillin, cefotaxime and kanamycin on morphogenesis of three Polish tomato cultivars, cultured on MS medium with phytohormones. In this experiment the ability of tomato cotyledon explants to regenerate entire plants via indirect organogenesis was tested. Among four antibiotics tested, kanamycin was most harmful for the explants. This antibiotic, even at low doses (10-20 mg/L), inhibited tomato morphogenesis. On the other hand, the current study revealed significant influence of different concentrations of ampicillin, carbenicillin, cefotaxime on the frequency of bud formation. While the addition of cefotaxime at low concentration (100-200 mg/L) stimulated the bud formation, its increasing concentration adversely affected the organogenesis of tomato. The results clearly pointed out that carbenicillin and ampicillin at low concentrations (100-400 mg/L) were not only non-toxic, but they promoted bud regeneration. The obtained results show the crucial role not only of use of efficient antibiotics, but also of their proper doses in obtaining successful transformation and regeneration of tomato.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Aneta GERSZBERG Izabela GRZEGORCZYK-KAROLAK Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311401 Phytochemical and Antioxidant Properties of Fresh Fruits and Some Traditional Products of Wild Grown Raspberry (Rubus idaeus L.) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11465 <p style="text-align: justify;">The current study investigated and compared phytochemical and antioxidant activity of fresh fruit and some traditional products of <em>Rubus idaeus</em> grown in mountain region of Serbia. The total organic acid, total sugar content, total phenolics, flavonoids, tannins, anthocyanins and vitamin C were evaluated. The antioxidant activities were evaluated using two antioxidant systems 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The fresh fruit contained highest amount of vitamin C (46.62 mg AA g<sup>-1</sup>) and total organic acids (882.22 mg CA g<sup>-1</sup>). The sweet preserve had highest content of total phenolics (200.83 mg GA g<sup>-1</sup>), flavonoids (12.85 mg RU g<sup>-1</sup>) and tannins (39.11 mg g<sup>-1</sup>). The juice had the highest total anthocyanin content (107.22 µg mL<sup>-1</sup>) and total sugar content (25 °Brix). The best antioxidant activity in ABTS assay had juice (IC<sub>50 </sub>= 4.87 µmol TE g<sup>-1</sup>), followed by sweet preserve (IC<sub>50 </sub>= 5.14 µmol TE &nbsp;g<sup>-1</sup>), almost identical to standard gallic acid. In the DPPH free radical scavenging assay, sweet fruit preserve showed significant better antioxidant activity (IC<sub>50</sub> = 41.27 µg mL<sup>-1</sup>) compared to juice (IC<sub>50</sub> = 106.07 µg mL<sup>-1</sup>) and fresh fruit (IC<sub>50</sub> = 294.79 µg mL<sup>-1</sup>). Our results indicated promising perspectives for usage of <em>R. idaeus</em> fresh fruits and traditional products studied with considerable levels of vitamin C, bioactive compounds and antioxidant activity.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Bojana VELJKOVIĆ Violeta JAKOVLJEVIĆ Milan STANKOVIĆ Zora DAJIĆ-STEVANOVIĆ Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311465 Non-Destructive Leaf Area Estimation Model for Overall Growth Performances in Relation to Yield Attributes of Cassava (Manihot esculenta Cranz) under Water Deficit Conditions https://www.notulaebotanicae.ro/index.php/nbha/article/view/11487 <p style="text-align: justify;">Cassava is a tropical storage root crop, a source of carbohydrate and alternative energy. It has been classified as “drought tolerant plant” for the whole life cycle, except during the root initiation stage (120-150 DAP). Leaf area index (LAI) is one of the most parameters representing the overall growth and yield prediction in cassava. The aim of this investigation was to validate the physiological and growth performance of cassava in responses to water deficit stress in the field trial as well as to investigate the leaf area index as an important factor to cassava growth and storage root bulking. Leaf relative water content in cassava declined significantly upon a long period of water withholding, and regulated non-photochemical quenching (NPQ), leading to chlorophyll degradation, reduced number of leaves and limited leaf area index (LAI) and loss of storage root yield when compared with well-irrigated plants. Non-destructive leaf area estimation model under water deficit stress condition using spectral reflectance to determine the LAI and VIs was validated. The Ratio Vegetation Index (RVI) was suitable model with high coefficient of determination (R<sup>2</sup> = 0.89). However, the RVI as LAI at 150 DAP (120 d water withholding) could be considered as the critical point to indicate cassava growth and yield performance. Based on the results, cassava growth, biomass and yield in the different environments may further be investigated, taking into consideration the genotypic variation and using remote sensing technology for rapid monitoring and accurate and cost-effective data assessment.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Piyanan PIPATSITEE Apisit EIUMNOH Patchara PRASEARTKUL Navavit PONGANAN Kanyarat TAOTA Sumaid KONGPUGDEE Kampol SAKULLEERUNGROJ Suriyan CHA-UM Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311487 The Productive Capacity and Quality of Several Walnut Cultivars (Juglans regia L.) Grown in North Oltenia, Romania https://www.notulaebotanicae.ro/index.php/nbha/article/view/11475 <p style="text-align: justify;">Romania has tradition in walnut culture and favorable climatic conditions. In order to establish the suitable cultivars for growing into the Northern Oltenia area several walnut cultivars of different origin were evaluated for productivity and fruits quality. Twelve Romanian cultivars and two foreign ones with terminal bearing and 10 cultivars with lateral bearing from France and U.S.A. were studied during 18 years of yielding with no irrigation. The group of lateral bearing cultivars proved to be more productive in the environmental conditions of the study area, the highest average yields were recorded for ‘Vina’ (3.64 t ha<sup>-1</sup>), ‘Hartley’ (3.59 t ha<sup>-1</sup>), ‘Ferjean’ (3.52 t ha<sup>-1</sup>), ‘Payne’ (3.45 t ha<sup>-1</sup>), ‘Pedro’ (3.39 t ha<sup>-1</sup>), etc. The most productive terminal bearing cultivars were: ‘Jupânești’ (2.88 t ha<sup>-1</sup>), ‘Valcor’ (2.77 t ha<sup>-1</sup>), ‘Franquette’ (2.71 t ha<sup>-1</sup>), ‘Valcris’ (2.62 t ha<sup>-1</sup>), etc. Although the lateral bearing cultivars emphasized good yields, their extensive culture into the areas with continental type of climatic conditions should be further analysed. The walnut kernel and oil content show that the cultivars are very similar regarding chemical composition when growing under same conditions in Northern Oltenia.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********<br></strong></p> Mihai BOTU Yazan F.J. ALABEDALLAT Felicia BUCURA Elisabeta Irina GEANA Marius VLADU Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311475 Priming with Vitamin U Enhances Cold Tolerance of Lettuce (Lactuca sativa L.) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11433 <p style="text-align: justify;">Priming may be an efficient pre-treatment of plants in order to enhance their ability to cope with unfavourable growth conditions, and to improve defensive metabolism through elevated levels of protective substances which may also act as health-promoting agents upon human consumption. The aim of this work was to evaluate the beneficial influence of priming with the naturally occurring, but scarcely known vitamin U (S-methylmethionine) on cold stress tolerance of lettuce (the frequently grown ‘May King’ cultivar). Effects on germination, photosynthetic efficiency, as well as on health-promoting carotenoid and vitamin C contents were investigated. Photosynthetic capacity, strongly related to productivity, was evaluated with parameters of induced chlorophyll fluorescence and of leaf gas exchange through stomata, using plants grown in hydroponic cultures. Priming with vitamin U significantly compensated for the delaying effect of low temperature (5 °C) on seed germination, as well as for inhibition of light-converting photochemical reactions and of carbon dioxide assimilation by cold stress. Use of vitamin U to prime lettuce plantlets for low temperature stress resulted in an elevated content of carotenoid pigments and of vitamin C in leaves, which improve the quality of consumed lettuce with respect to the health-promoting capacity. This beneficial influence of vitamin U was not proportional with its concentration (2 mM had no stronger effects than 0.25 mM), so small amounts of this substance were sufficient for a sustained efficiency in promoting hardening against chilling temperatures. This is the first report on priming of lettuce for cold tolerance by using S-methylmethionine (vitamin U), with a possible application in improvement of crop quality and productivity.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Laszlo FODORPATAKI Katalin MOLNAR Bernat TOMPA Sebastian R. C. PLUGARU Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311433 Biochemical Characterisation and Sensory Evaluation of Differently Coloured and Shaped Tomato Cultivars https://www.notulaebotanicae.ro/index.php/nbha/article/view/11382 <p style="text-align: justify;">Antioxidant capacity, total phenolic content, colour, sugar, volatiles, ascorbic acid and carotenoid (β-carotene and lycopene) contents of differently coloured and shaped tomato cultivars (cvs) grown in the Eastern Mediterranean region, Turkey were determined, along with a sensory evaluation. Tomato cultivars of two different types (cherry and beefsteak) and four different colours (red, yellow, orange and brown) were analysed. All plants were simultaneously grown in the same field and subjected to identical horticultural practices to minimise the effects of environmental conditions and to maximise those related to genotype. The red cherry cultivar had the highest lycopene content, while the orange beefsteak cultivar had the highest β-carotene content. The highest antioxidant capacity, total phenolic content and hardness scores were found in cherry-type tomatoes, except the yellow one. The red cherry cultivar had the highest sugar content. Red and brown cherry cultivars were also significant in terms of their high carotenoid and sugar contents, along with a high antioxidant activity. The brown cherry cultivar had the highest total phenol content. The highest quantities of 2-hexenal, 3-hexen-1-ol, and 6-methyl-5-hepten-2-one were detected in red cultivars. The brown cherry cultivar had the highest sweetness, typical aroma and hardness scores, while the yellow beefsteak cultivars the lowest sweetness typical aroma scores. In terms of sensory parameters, red and brown cultivars scored higher than yellow and orange ones.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Aylin Celile OLUK Atilla ATA Mustafa ÜNLÜ Ebru YAZICI Zafer KARAŞAHİN Evren Çağlar EROĞLU İhsan CANAN Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311382 Arbuscular Mycorrhiza Improves Leaf Food Quality of Tea Plants https://www.notulaebotanicae.ro/index.php/nbha/article/view/11434 <p style="text-align: justify;">Tea (<em>Camellia sinensis</em>) plants inhabit arbuscular mycorrhizal fungi (AMF) in rhizosphere, whereas it is not clear whether AMF improves leaf food quality of tea plants. A potted study was conducted to determine effects of <em>Claroideoglomus etunicatum,</em> <em>Diversispora</em> <em>spurca</em>, <em>D</em>. <em>versiformis </em>and a mixture of the three AMF species on leaf sugar, amino acid, soluble protein, tea polyphenol, catechuic acid, and flavonoid contents of <em>Camellia sinensis</em> ‘Fuding Dabaicha’ seedlings. After 12 weeks of AMF inoculation, mycorrhizal plants recorded significantly higher shoot biomass and total leaf area, whilst the effect was ranked as <em>C</em>.<em> etunicatum</em> &gt; <em>D</em>. <em>spurca </em>&gt; mixed-AMF &gt; <em>D</em>. <em>versiformis</em> in the decreasing order. AMF treatments significantly increased leaf total amino acid concentrations, accompanied with up-regulation of amino acid synthetic enzymes genes glutamine synthetase (<em>CsGS</em>), glutamate synthase (<em>CsGOGAT</em>) and glutamate dehydrogenase (<em>CsGDH</em>). Leaf glucose, sucrose, total soluble protein, tea polyphenol, catechuic acid, and flavonoid contents were significantly higher in AMF- than in non-AMF-inoculated plants. In addition, mycorrhizal inoculation notably up-regulated the expression level of leaf 3-hydroxy-3-methylglutaryl coenzyme gene (<em>CsHMGR</em>)<em>, </em>ascorbate peroxidase gene (<em>CsAPX</em>), and tea caffeine synthase 1 gene (<em>CsTCS1</em>). These results implied that AMF inoculation had positive effects on leaf food quality partly by means of up-regulation of relevant gene expression in ‘Fuding Dabaicha’ seedlings.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Ya-Dong SHAO De-Jian ZHANG Xian-Chun HU Qiang-Sheng WU Chang-Jun JIANG Xiu-Bing GAO Kamil KUČA Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311434 Investigation of Mineral and Vitamin C Contents in Different Parts of Beetroots (Beta vulgaris L.) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11394 <p style="text-align: justify;">Due to the increasing popularity of today’s health-conscious culture, beetroot plays an increasingly important role in a healthy, varied diet. While it contains vitamins B1, B2, B6, P, as well as high levels of vitamin C, several studies have proven its role in the prevention of many cancers. The aim of this study was to compare morphological properties, dry matter, and vitamin C and minerals contents in the different parts (peel, end, middle and top part of root) of the three investigated beetroot cultivars grown in treated and untreated soil. Fertilizer was added to the treated soil before sowing of the beet seeds, while untreated soil was not treated. The vitamin C contents were measured using HPLC method, while determination of element contents was determined with IPC-OES. The study showed that the beetroots grow in the treated soil had improved morphological properties, but a significant difference was not found. For Vitamin C and element contents (Ca, Cu, Fe, K, Mg, Mn, Na, P, Zn), it was observed a high degree of diversity in the different beetroot parts and between the cultivation years. The aim of the study was to investigate the distribution of the examined parameters within the beetroot body in different beetroot cultivars observing the differences produced by the addition of fertilizer. Some elements were concentrated in the peel and top part such as Ca, Mn and Mg. The peel part of the beetroots contained relevantly higher Fe content than the top, middle and end part. Based on these results, significant differences were observed among the investigated beetroot cultivars regards of the body weight.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Dóra SZÉKELY Diána FURULYÁS Mónika STÉGER-MÁTÉ Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311394 Phytosociology, Distribution and Ecology of a Willow Community with False Tamarisk from the Lotru Valley (Romanian Carpathians) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11400 <p style="text-align: justify;">The overall objective of this research was to contribute to a better knowledge of the phytosociology, ecology and distribution of the plant community formed by <em>Myricaria germanica</em> (false tamarisk) and the willow species <em>Salix purpurea.</em> This plant community has an important role in reducing the speed and strength of the water flow during floods. The description was based on cover‐abundance data for the faithful, dominant, characteristic and companion species of this plant community. A predominance of <em>Myricaria germanica</em> was observed when analysing the phytocoenoses, as the species finds in this area favourable ecological conditions for an abundant development.<em> Salix purpurea</em> presents a small abundance-dominance or in some phytocoenoses may even lack. The field research also revealed the excessive development of the species <em>Calamagrostis pseudophragmites</em> in some areas, related mostly with the effect of human activities. The anthropogenic disorders have induced modifications in the floristic physiognomy and compositions of the phytocoenoses, favouring also the invasion of opportunistic species such as <em>Ambrosia artemisiifolia</em>,<em> Impatiens glandulifera</em>,<em> Phytolacca amearicana</em>, <em>Amorpha fruticosa</em>,<em> Erigeron annuus</em>, which continuously extend in the studied territory. As <em>Myricaria germanica</em> and <em>Salix purpurea</em> phytocoenoses suffer a great decline throughout Europe, the habitat built by these species was declared of interest and was included in the Habitats Directive.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Ilie Silvestru NUȚĂ Mariana NICULESCU Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311400 Optimization of the Solid-Liquid Extraction Process of Phenolic Compounds from Mulberry Fruit https://www.notulaebotanicae.ro/index.php/nbha/article/view/11419 <p style="text-align: justify;">The study was designed to examine the influence of solvent concentration (ethanol/water 20-80% by volume), extraction time (15-240 min) and extraction technique on the extraction yield of phenolic compounds, flavonoids and antioxidant activity from <em>Morus nigra</em> L., <em>Morus rubra</em> L. and <em>Morus alba</em> L. fruits. The optimum conditions for the extraction of total phenols, flavonoids and monomeric anthocyanins by the maceration and ultrasonic extraction processes were: 213.6 min with 80% ethanol, and 182.1 min and 71.2% ethanol, respectively (black mulberry), and 216.5 min with 78.3% ethanol and 198.7 min and 70.6% ethanol, respectively (red mulberry). In case of white mulberry for the extraction of total phenols and flavonoids optimum extraction conditions (maceration and ultrasonic) were 232.7 min and 80% ethanol, and 187.2 min and 68.7% ethanol, respectively (white mulberry). Obviously, ultrasonic extraction was less time consuming, and it requires for all performed extractions solvent with less percentage of ethanol.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Emilija KOSTIĆ Biljana ARSIĆ Milan MITIĆ Danica DIMITRIJEVIĆ Emilija PECEV MARINKOVIC Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311419 Antioxidant Properties and Anti-inflammatory Effects of the Hydroethanolic Extracts of Two Varieties of Bayberry fruit (Myrica rubra Sieb et Zucc.) Prepared by Stirring and Ultrasonic Methods https://www.notulaebotanicae.ro/index.php/nbha/article/view/11420 <p style="text-align: justify;">Chinese bayberry (<em>Myrica rubra </em>Sieb. et Zucc.) is an economically important medicinal plant with multiple uses. Two varieties ‘Dongkui Oriental Pearl’ (Dongkui for short) and <em>acuminata</em> ‘Nakai’ (Nakai for short) were used to compare and evaluate the antioxidant activities of hydroethanolic extracts of the fruit using ultrasonic and stirring extraction methods. Dongkui bayberry fruit extract (BFE) prepared using the ultrasonic method exhibited a significantly higher value for the total phenolic content (TPC) and had lower 50% inhibitory concentration (IC<sub>50</sub>) values of scavenging activities against 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), as well as reducing power compared to the other treatment. The TPC of the BFE was significantly correlated with its DPPH, ABTS, and H<sub>2</sub>O<sub>2</sub> radical-scavenging and reducing power activities. Dongkui BFE at a concentration of 0.25 mg/mL exhibited significantly greater protection of RAW 264.7 mouse macrophages against H<sub>2</sub>O<sub>2</sub>-induced damage and lipopolysaccharide (LPS)-stimulated nitric oxide production by macrophages, and it displayed remarkable inhibitory effects compared to the other extracts using the ultrasonic extraction method. Furthermore, compared to the Nakai BFE, macrophages exposed to the Dongkui BFE by the ultrasonic extraction method significantly inhibited LPS-induced production of tumor necrosis factor-α at a concentration of the extract of 0.25 mg/mL. The antioxidant properties and anti-inflammatory and protective effects of BFE prepared by stirring and ultrasonic methods are discussed for the first time in this study.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Kuan-Hung LIN Chun-Ping LU Jia-Wei CHAO Yi-Ping YU Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311420 Composition of Lavandula angustifolia L. cultivated in Transylvania, Romania https://www.notulaebotanicae.ro/index.php/nbha/article/view/11565 <p style="text-align: justify;">An increasing market demand concerning lavender essential oil is reported. Identification and characterization of the main compounds of this oil is needed, in order to identify chemotypes. This information is useful for further uses of the produced oil. Essential oil extracted from two <em>Lavandula angustifolia </em>L. varieties, cultivated in different areas of Transylvania, Romania, was analysed from the point of view of qualitative and quantitative composition, using gas-chromatography. Basic statistics was used for calculation of essential oil compounds means and dispersion parameters, while the profile of the essential oil composition was emphasized using Box-Plot diagrams and cluster analysis. Nine compounds, in different amounts were quantified, and two chemotypes, corresponding to ‘Mailette’ and ‘Vera’ varieties were established (linalool, and linalyl acetate, respectively). Differences in essential oil composition are recorded in ‘Vera’ variety corresponding to ‘Vera’ linalyl acetate chemotype, function of specific condition of lavender cultivation area.</p> Camelia OROIAN Antonia ODAGIU Csaba Pal RACZ Ioan OROIAN Iulia C. MUREȘAN Marcel DUDA Marioara ILEA Ioan BRAȘOVEAN Cristian IEDERAN Zamfir MARCHIȘ Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-06-11 2019-06-11 47 3 10.15835/nbha47311565 Tyrosinase Inhibitory and Antioxidant Activity of Wild Prunus spinosa L. Fruit Extracts as Natural Source of Bioactive Compounds https://www.notulaebotanicae.ro/index.php/nbha/article/view/11425 <p style="text-align: justify;">In recent years, naturally derived tyrosinase inhibitors and antioxidants have become very important, since the synthetic ones are related with several side effects. As wild fruits are rich source of bioactive compounds, this study focused on the examination of <em>Prunus spinosa </em>L. (blackthorn) fruit extracts, widely distributed in our country. However, blackthorn extracts are not enough investigated, and there are only a few published works. Extraction efficiency of <em>P. spinosa</em> ultrasonic fruit extracts obtained by using different solvents (methanol, 70% (v/v) ethanol, 45% (w/w) propylene glycol and water) was evaluated, regarding polyphenolic profile, tyrosinase inhibitory and antioxidant activity. Results suggested that extracts with 45% propylene glycol and water were particularly rich in polyphenolic compounds (especially phenols, flavonoids and anthocyanins). The highest extraction yield was for 45% propylene glycol extract. This extract showed higher tyrosinase inhibitory and antioxidant activity compared to others, while the methanolic extracts had the highest chelating capacity. Therefore, <em>P. spinosa</em> fruit extracts, especially extracts obtained by ultrasonic extraction with 45% propylene glycol, have a great potential to be incorporated in some pharmaceutical formulations for obtaining tyrosinase inhibitory and antioxidant effects.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Milica I. STANKOVIĆ Vesna Lj. SAVIĆ Jelena V. ŽIVKOVIĆ Vanja M. TADIĆ Ivana A. ARSIĆ Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311425 Physiological Responses and Tolerance Evaluation of Five Poplar Varieties to Waterlogging https://www.notulaebotanicae.ro/index.php/nbha/article/view/11440 <p style="text-align: justify;">Waterlogging resistance of five poplar varieties, ‘Danhongyang’ (DHY), ‘Juba-261’ (JB-261), ‘Zongqiansanhao’ (ZQ-3), ‘Zhonglin-2025’ (ZL-2025), and ‘Nanlin-895’ (NL-895), was evaluated under the simulated waterlogging conditions. Data on changes in leaf color and morphology as well as in biochemical indices, such as chlorophyll, malonaldehyde, soluble protein, soluble sugar content, superoxide dismutase (SOD), peroxidases (POD), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione peroxidase (GSH-PX) activities, relevant to submergence stress, were analyzed. The principal component analysis of the data identified the waterlogging resistance coefficient of the indices, which showed that waterflooding brought about different degrees of damage in the five poplar varieties, with DHY having the lowest waterlogging index. The leaf pigment content of the poplar was remarkably decreased by waterlogging, whereas malondialdehyde (MDA) and proline contents were enhanced, but in different extents among the poplar varieties. Nearly all other poplar varieties showed a tendency of decline in JB-261, ZQ-3, ZL-2025, NL-895, except for SOD activity in DHY, which increased under submergence stress. Poplar varieties had varying degree of changes in POD activity, and APX activity tended to increase upon waterlogging. GR also displayed increasing tendency in JB-261, ZL-2025 and NL-895, except for in ZQ-3, which declined under waterlogging stress. GSH-PX except for ZQ-3 displayed no significant change, which showed a tendency of decline in DHY, JB-261, ZL-2025, and NL895. Principal component analysis allowed us to reduce16 indices to four independent indices. The subordinate function analysis identified that the DHY variety had the highest waterlogging tolerance, whereas the NL-895 variety had the lowest waterlogging tolerance among tested varieties.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Zhongcheng ZHOU Gang LI Wei CHAO Feng XU Xiaomei SUN Zexiong CHEN Copyright (c) 2019-04-10 2019-04-10 47 3 10.15835/nbha47311440 Biocontrol of Pear Postharvest Decay by Kombucha https://www.notulaebotanicae.ro/index.php/nbha/article/view/11407 <p style="text-align: justify;">Kombucha is sweetened, slightly alcoholic, and lightly effervescent tea drink. Its acidity inhibits the growth of harmful bacteria. Here, we studied effect of kombucha on post-harvest preservation of pear. The Hosui pears were soaked with the kombucha for 15 minutes, and in distilled water as the control, respectively. Superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), proline content, electric conductivity, weight loss rate and good fruit rate in pear were measured during storage at room temperature. The results showed that the kombucha could effectively prolong the storage time of pear fruit. The fruit quality of the treated group was 1.5 times higher than that of the control pear fruit when stored at room temperature for 18 days. The weight loss rate, MDA content and electric conductivity of the treatment group were lower than those of the control group during the whole storage period, indicating that kombucha can inhibit the transformation of polysaccharides such as starch and pectin, and delay the degradation of nutrients in the fruit, resulting in a decrease in weight loss rate, inhibition of membrane lipid peroxidation, reducing MDA content, electric conductivity, maintaining cell membrane stability, delaying pear fruit senescence. Kombucha can inhibit the content of H<sub>2</sub>O<sub>2</sub> and proline, increase the activity of POD and SOD increase the storage stability of fruits. The purpose of this study was to elucidate the physiological mechanism of post-harvest preservation of pear by kombucha. The use of kombucha can prolong the supply period of pear, increase economic benefits and expand the market of pear.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;"><strong>*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;"><strong>*********</strong></p> Junping TAN Xian ZHOU Shuiyuan CHENG Zexiong CHEN Yuanyuan GOU Jiabao YE Feng XU Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-04-17 2019-04-17 47 3 10.15835/nbha47311407 Cross Compatibility Analysis to Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding https://www.notulaebotanicae.ro/index.php/nbha/article/view/11388 <p style="text-align: justify;">Seven interspecific crosses of <em>Tagetes erecta</em> × <em>T. patula</em> were conducted in this study<strong>. </strong>The cross compatibility index, seed setting rate, germination rate, phenotype, ploidy level, heterosis over male parent and field performance were tested. The results showed that different cross compatibility indices of <em>Tagetes</em> vary greatly, from 10.44~114.41, which is far less than that of the intraspecific hybridization S-121 × I-506 index. The seed setting rate of each cross ranged from 15.67% to 49.0%, and the cross S-121 × TP-512 scored the highest seed setting rate, which was higher than that of the intraspecific cross S-121 × I-506 (35.85%). Compared with the male parent, F<sub>1</sub> has higher plant height, wider crown width, larger flower diameter and more ray florets and earlier bloom with two exceptions. The S-121 of <em>T. erecta</em> was diploid. All of the male parents of <em>T. patula </em>were tetraploids, and the combinations were triploids. The traits of plant height, plant width, flower size and number of ray florets showed positive heterosis over the male parents, and the days leading to flowering showed both positive and negative ones. Finally, S-121 × TP-379 and S-121 × TP-512 were selected as the two best combinations. This study confirmed that the two species of marigold could be crossed for better F<sub>1</sub> varieties with improved performance.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Huali ZHANG Haibo XIN Richen CONG Zijing LI Lina SONG Zunzheng WEI Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-04-17 2019-04-17 47 3 10.15835/nbha47311388 Physiological Effects of the Fungicide Azoxystrobin on Wheat Seedlings under Extreme Heat https://www.notulaebotanicae.ro/index.php/nbha/article/view/11448 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Azoxystrobin (AZ) is not only a fungicide used for disease control, but also a protective chemical for crops against specific stresses. The physiological mechanism of the fungicide AZ in protecting against heat (HT, 46 °C) stress in wheat (<em>Triticum aestivum</em> L.) seedlings was investigated. ‘Taichung SEL-2’ variety seedlings were pretreated with 0.4, 4, 40, 80, and 120 mg L<sup>-1</sup> of AZ for 4 d. Next AZ-pretreated and untreated seedlings were subjected to HT for 1 h followed by 1000 μmol m<sup>-2 </sup>s<sup>-1</sup> lighting for 20 min. HT induced oxidant stress which resulted in a decrease in the reducing power, an increase in malondialdehyde, and enhanced enzyme activities of ascorbate peroxidase (APX) and catalase (CAT) in leaves of untreated seedlings. However, AZ-pretreated seedlings under HT displayed reductions in chlorophyll fluorescence, APX and CAT activities, and the 1,1-diphenyl-2-picryl-hydrazyl scavenging capacity. Physiological damage caused by HT was aggravated by an increase in the AZ concentration. In addition, increased photosynthetic pigments were also observed in leaves of AZ-pretreated and HT-exposed seedlings. The results suggest that AZ does not provide a protective effect against HT stress.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Chiu-Yueh LAN Kuan-Hung LIN Wen-Dar HUANG Chang-Chang CHEN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-14 2019-05-14 47 3 10.15835/nbha47311448 The Propagation of Rosa gallica ‘Tuscany Superb’ by Root Cuttings with the Use of IBA and Biostimulants https://www.notulaebotanicae.ro/index.php/nbha/article/view/11436 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">The research was aimed at studying the possibility of an alternative method of propagation the difficult-to-root <em>Rosa gallica</em> ‘Tuscany Superb’. The root cuttings of three thickness were rooted using IBA preparations (Chryzotop green, Rhizopon AA 020XX) and biostimulants (Tytanit, Root Juice<sup>TM</sup>). The shoots disappeared before the growth of the roots. At the first days of growth, the both shoots and roots elongated upright without polarity. The primordia of the first type of root/shoot buds were situated in cambium zone as the subtending traces of near the rays visible on the cross sections. Moreover, the second types of buds were located in pericycle tissues. The swellings suggested inherence buds were disappearing before rooting. The content of reducing and non-reducing carbohydrates was lower at the time of harvesting the rooted cuttings than before rooting. The thickness of the root cuttings had a significant importance for growth parameters and content of carbohydrates in rooted cuttings. The most useful root cuttings, those achieving the highest mean rooting percentage are thick (76.8%) and medium (67.9%) size. Preparations or biostimulants had a differential influence on rooting process and none of them unequivocally guaranteed an improvement in the rooting percentage of cuttings, parameters of growth or content of carbohydrates. The Rhizopon AA 020XX (90.0%) in thick and Chryzotop green (87.5%) in medium cuttings increased the rooting percentage significantly. Root Juice<sup>TM</sup> (0.1%, 0.2%) and Tytanit (0.04%) were also enough positive activity in medium cuttings and can replace IBA preparations.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Marta Joanna MONDER Konrad WOLIŃSKI Maciej NIEDZIELSKI Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-15 2019-05-15 47 3 10.15835/nbha47311436 Low Level of Genetic Variation and Signs of Isolation in the Native Hungarian Sea Buckthorn Population Compared to Cultivated Specimens https://www.notulaebotanicae.ro/index.php/nbha/article/view/11333 <p style="text-align: justify;">Sea Buckthorn population from Újpest Nature Reserve, the only one assumed to be native in Hungary sustains at the periphery of Budapest city on the sandy substrate deposited by the Danube river. The study aims to characterize the gene stock preserved on this small edaphic habitat by comparing with the genetic material of planted specimens of known origin used in horticultural and gardening practice. Fragment length variations of seven nuclear microsatellite markers were evaluated and a phylogenetic tree was constructed based on sequence variation of an intron from nuclear gene encoding the chalcone synthase enzyme. Gender rate was evaluated by female specific SCAR marker. Microsatellite analysis pointed out the extremely low variation of the natural sea buckthorn population from Újpest Nature Reserve compared to the cultivated specimens, while the phylogenetic tree revealed that the natural population belongs to the genetic lineage distributed mostly in Western and Middle-Europe, and in Romania, region, which has strong links to the Balkan and the Black Sea. The analysis clearly separated the genetic material of the native Újpest population from the planted individuals from the vicinity that proved to be of allochthonous origin. The HrX2 SCAR marker revealed the dominance of the males (1:1.42). The study provides support for the autochthonous origin of the Hungarian remnant population being in reproductive isolation that suggest for its long persistence and relict status. <em>Ex situ</em> conservation and nature protection measures are highly recommended to maintain one of the last native sea buckthorn populations of the Pannonian region.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> Mária HÖHN Magdolna SZELÉNYI Júlia HALÁSZ Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-15 2019-05-15 47 3 10.15835/nbha47311333 Growth, Physiological and Biochemical Responses of two Greek Cotton Cultivars to Salt Stress and their Impact as Selection Indices for Salt Tolerance https://www.notulaebotanicae.ro/index.php/nbha/article/view/11463 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">Soil salinity is a major constrain of crop productivity. Upland cotton (<em>Gossypium hirsutum</em> L.) is an important fiber crop worldwide and a major agricultural product in Greece. Two commercial cotton cultivars (‘Hersi’ and ‘ST 318’) were studied to compare their response under non-saline and saline conditions in a greenhouse experiment. Salt stress on plants was imposed by two different approaches: a gradual and an initial acclimatization to a non-lethal NaCl concentration (150 mM). To explore salt stress responses, growth (height of plants, roots, shoots and leaves dry weight, reproductive shoots, Salinity Sensitivity Index), gas exchange (Photosynthetic rate, Stomatal conductance, Transpiration rate and Water Use Efficiency) and biochemical parameters (proline, H<sub>2</sub>O<sub>2</sub> and MDA content), were examined as well as ion homeostasis. ‘Hersi’ had significantly higher dry weight of roots, shoots and leaves, lower salinity sensitivity index of roots compared to ‘ST 318’.&nbsp; In this regard, it appears that ‘Hersi’ cultivar performed better than ‘ST 318’ to increased salinity conditions, due to better control of gas exchange parameters and K<sup>+</sup>/Na<sup>+</sup> homeostasis as well as better membrane integrity. Furthermore, the gradual acclimatization to the 150 mM NaCl concentration had a milder effect on both cultivars compared to the initial acclimatization.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Maria-Anna MOUSSOURAKI Eleni TANI Anna VELLIOU Maria GOUFA Maria PSYCHOGIOU Ioannis E. PAPADAKIS Eleni M. ABRAHAM Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-15 2019-05-15 47 3 10.15835/nbha47311463 Effects of Different Shading Rates on the Photosynthesis and Corm Weight of Konjac Plant https://www.notulaebotanicae.ro/index.php/nbha/article/view/11437 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">To study the effects of shading level on the photosynthesis and corm weight of konjac plant, the chlorophyll fluorescence parameters, daily variation of relative electron transport rate (rETR), net photosynthetic rate (<em>P</em>n), and corm weight of konjac plants under different treatments were measured and comparatively analyzed through covered cultivation of biennial seed corms with shade nets at different shading rates (0%, 50%, 70%, and 90%). The results showed that with the increase in shading rate, the maximum photochemical efficiency, potential&nbsp;activity, and non-photochemical quenching of photosystem Ⅱ (PSⅡ) of konjac leaves constantly increased, whereas the actual photosynthetic efficiency, rETR, and photochemical quenching of PSⅡ initially increased and then decreased. This result indicated that moderate shading could enhance the photosynthetic efficiency of konjac leaves. The daily variation of rETR in konjac plants under unshaded treatment showed a bimodal curve, whereas that under shaded treatment displayed a unimodal curve. The rETR of plants with 50% treatment and 70% treatment was gradually higher than that under unshaded treatment around noon. The moderate shading could increase the <em>P</em>n of konjac leaves. The stomatal conductance and transpiration rate of the leaves under shaded treatment were significantly higher than those of the leaves under unshaded treatment. Shading could promote the growth of plants and increase corm weight. The comprehensive comparison shows that the konjac plants had strong photosynthetic capacity and high yield when the shading rate was 50%-70% for the area.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Yaoguo QIN Zesheng YAN Honghui GU Zhengxiang WANG Xiong JIANG Zhizhou CHEN Fuqing YANG Cuiqin YANG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-19 2019-05-19 47 3 10.15835/nbha47311437 Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis https://www.notulaebotanicae.ro/index.php/nbha/article/view/11450 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"><em>Trichosanthes kirilowii</em> Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for <em>in vitro</em> production of high-quality plantlets of <em>T. kirilowii</em> cv. ‘Yuelou-2’ via organogenesis<em>. </em>Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L<sub>9 </sub>(3<sup>4</sup>) orthogonal array design. MS medium supplemented with 0.5 mg l<sup>-1</sup> 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance <em>in vitro</em> and <em>ex vitro</em> were comprehensively assessed, and results showed that the addition of 1.0 mg l<sup>-1 </sup>NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The <em>in vitro</em> propagation system described here has the potential for large-scale and rapid production of <em>in vitro</em> plantlets of seed variety of <em>T</em>. <em>kirilowii</em> in a sustainable manner.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Xiao Dong CAI Jin Jin CHENG Yuan Shan ZHANG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-19 2019-05-19 47 3 10.15835/nbha47311450 Assessment of New Sweet Cherry Cultivars Released at RSFG Iaşi, Romania https://www.notulaebotanicae.ro/index.php/nbha/article/view/11575 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">The research concerning the improvement of the cherry assortment, the cultivars’ quality and the avoiding of market crowding with fresh cherries only on a short term are extremely important objectives for cherries’ breeding. To accomplish some of these objectives, during 1981-2017, hybridization and selection of hybrids were performed at the Research Station for Fruit Growing Iași (RSFG Iași), in the N-E part of Romania, concluding with a series of promising hybrid selections. They have been grafted, planted and studied in trial fields. Therefore, during 1994-2016, RSFG Iași released 28 sweet cherry cultivars with high quality fruits and different maturation stages. In the studies performed during 2014-2017, there were analysed three new sweet cherry cultivars (‘Elaiaşi’, ‘Muşatini’ and ‘Croma’), obtained through controlled hybridization. The comparison of these new cultivars was performed against ‘Van’, used as control, this being a well-known cultivar in the orchards from the study area. Observations and measurements concerning the main phenological stages, natural fertility, physical features, organoleptic, quality and chemical traits of fruit were registered. The cultivars with the biggest dimensions of the fruits were ‘Elaiaşi’ and ‘Croma’ (with a weight between 8.9 and 9.4 g and a fruits’ diameter between 25.2 and 26.6 mm), having significantly larger dimensions in comparison with ‘Van’. In the new investigated cherry cultivars, the values of soluble solids content were comprised between 17.0 and 20.2 °Brix, titratable acidity was between 0.46 - 0.57 mg of malic acid 100 mL<sup>-1</sup> juice and the total polyphenols content was recorded between 382.87 - 780.25 mg GAE 100 mL<sup>-1</sup> of fresh juice.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Elena IUREA Margareta CORNEANU Mădălina MILITARU Sorina SÎRBU Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-21 2019-05-21 47 3 10.15835/nbha47311575 Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate https://www.notulaebotanicae.ro/index.php/nbha/article/view/11486 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Lilium<em> candidum</em> L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of <em>L. candidum</em> are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective <em>in vitro </em>plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 µM Thidiazuron + 1.08 µM Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 µM TDZ + 1.08 µM NAA were successfully rooted using 1/2 × MS medium containing 2.45 µM indole butyric acid. Regardless of rooting development, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Sheida DANESHVAR ROYANDAZAGH Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-20 2019-05-20 47 3 10.15835/nbha47311486 Optimization and Characterization of Extracellular Cellulase Produced by Native Egyptian Fungal Strain https://www.notulaebotanicae.ro/index.php/nbha/article/view/11500 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">Since accumulation of agricultural wastes represents a huge problem, it was important to explore the available methods to help eliminate agricultural wastes safely, and simultaneously produce functional enzyme like cellulase. Six native Egyptian fungal strains were isolated, morphologically identified and screened for cellulose biodegradation potential, which was determined as endoglucanase or as carboximethylcellulase (CMCase). The most promising isolate (<em>Aspergillus terreus</em>) was selected for molecular characterizations based on sequencing of internal transcribed spacer (ITS). Further optimization experiments were accomplished on the selected strain. The strain with cellulolytic activity, 2.26 IU mL<sup>-1</sup> was identified using ITS nucleotides (genes) sequences and the result confirmed that the strain is 99.8% homology with<em> A. terreus.</em> Then, it was submitted to GeneBank and given an accession number. Further optimization experiments revealed that 35ºC is the optimum temperature for cellulase production and raised the enzyme activity (EA) up to 3.19 IU mL<sup>-1</sup>. Out of two organic nitrogen sources; peptone at concentration 6 gL<sup>-1</sup> was found to be the optimum nitrogen source for cellulase production with the highest activity 4 IU mL<sup>-1</sup>. Whereas, the different four carbon sources: microcellulose, corn stalks, wheat straw and rice straw showed significant differences in EA with values 11.07, 9.68, 7.87 and 3.71 IU mL<sup>-1</sup>, respectively at&nbsp; pH 3. The maximum EA was recorded to be within 5-7 days of incubation, dependent on type of carbon sources. The optimization of different incubation conditions raised cellulolytic activity from 2.26 IU mL<sup>-1</sup> up to 11.18 IU mL<sup>-1</sup>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> Gamal El-BAROTY Faten ABOU-ELELLA Hassan MOAWAD Talaat N. El-SEBAI Fatma ABDULAZIZ A. A. KHATTAB Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-21 2019-05-21 47 3 10.15835/nbha47311500 Estimating Nitrogen and Chlorophyll Status of Romaine Lettuce Using SPAD and at LEAF Readings https://www.notulaebotanicae.ro/index.php/nbha/article/view/11525 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Nitrogen (N) is an essential nutrient for plant growth and development and is especially important in the production of high quality leafy green vegetables. In this experiment, leaf N concentration, chlorophyll concentration (Chl) and weight above fresh matter (AFM) of romaine lettuce (<em>Lactuca sativa</em> L. var. <em>longifolia</em>) were estimated by correlations between <em>in situ</em> SPAD and atLEAF readings. Lettuce was grown in high tunnels during 42 days and was irrigated at five nitrogen levels: 0, 4, 8, 12 and 16 mEq·L<sup>-1</sup> of NO<sub>3</sub><sup>-</sup>, based on the Steiner nutrient solution. The N concentration, Chl concentration and AFM were determined in the laboratory, while SPAD and atLEAF readings were measured <em>in situ</em> weekly. SPAD readings had high, positive and significant linear correlations with N (R<sup>2</sup> = 0.90), Chl (R<sup>2</sup> = 0.97) and AFM (R<sup>2</sup> = 0.98); atLEAF readings had a similar linear correlation with N (R<sup>2</sup> = 0.91), Chl (R<sup>2</sup> = 0.92) and AFM (R<sup>2</sup> = 0.97). Besides, SPAD and atLEAF readings had high, positive, and significant linear correlation (R<sup>2</sup> = 0.96). Thus, SPAD and atLEAF meters can be used to non-destructively and accurately estimate the N status of lettuce, in a reliable and quick manner during the crop production cycle. In addition, atLEAF is currently more affordable than SPAD.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Rodrigo Omar MENDOZA-TAFOLLA Porfirio JUAREZ-LOPEZ Ronald-Ernesto ONTIVEROS-CAPURATA Manuel SANDOVAL-VILLA Iran ALIA-TEJACAL Gelacio ALEJO-SANTIAGO Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-22 2019-05-22 47 3 10.15835/nbha47311525 Various Light Quality including QD-LED Affect Growth and Leaf Color of Red Romaine Baby Leaf Lettuce https://www.notulaebotanicae.ro/index.php/nbha/article/view/11580 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">Light quality has a substantial effect on crops in plant factories. Quantum dot (QD) refers to ultrafine semiconductor particles and is expressed in a variety of wavelength ranges and fine and precise colors and is attracting attention as a next-generation material. This study was conducted to investigate the effects of various light qualities, including a QD-LED light source on the growth and color development of red romaine lettuce in the LED chamber system for plant factories. The light source was red fluorescent (FL), blue (B), red (R), blue + red mixed light (BR), and blue + wide range red + red mixed light QD-LED. The growth index, chlorophyll content, and leaf color were examined. The results showed that the plant length was long without a significant difference in QD-LED and the red (R) wavelength and showed the fastest growth under QD-LED. The photosynthetic rate and chlorophyll content were the highest in QD-LED light with a broad spectrum of red light. The leaf colors a*, b*, the hue angle, and total anthocyanin content showed the highest in QD-LED light, while b* and the hue angle values ​​were the lowest. As the light wavelength range was changed, there was a significant difference in the growth response and leaf color, and it is considered that QD-LED light should be considered positively when applied to plant factories.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> Su Jung HAN In-Lee CHOI Ju Young KIM Lixia WANG Joo H. LEE Ki-Young CHOI Yongduk KIM Mohammad Z. ISLAM Young-Tack LEE Ho-Min KANG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-28 2019-05-28 47 3 10.15835/nbha47311580 Antioxidant Effects of Walnut Leaves and Sweet Cherry Stems on Color, Lipid Oxidation and Sensory Quality of Cooked Pork Patties https://www.notulaebotanicae.ro/index.php/nbha/article/view/11458 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">The effects of walnut leaf powder (WLP), walnut leaf extract (WLE) and cherry stem extract (CSE) on the oxidative and color stability of cooked pork patties were determined and compared with 0.01% butylated hydroxytoluene (BHT) and control (without antioxidant). Instrumental color, pH, total phenolic content, antioxidant activity, thiobarbituric acid reactive substances (TBARS) and sensory attributes were determined on manufactured patties initially (time 0) and after 5, 10 and 15 days of refrigerated storage. Addition of walnut leaf extract to pork patties was more effective than cherry stem extract in controlling lipid oxidation and color changes during cold storage, in agreement with results from the <em>in vitro</em> antioxidant activity. The natural additives significantly (p&lt;0.05) increased phenolic content and antioxidant activity in cooked pork patties as compared to control and BHT treated samples, throughout the storage period. After 15 days, the TBARS values were significantly (p&lt;0.05) reduced from 3.01 in control patties to 2.43, 1.58, 1.83 and 1.19 mg malondialdehyde per kg samples in patties with BHT, WLE, CSE and WLP, respectively. Addition of extracts showed positive effects on sensory attributes, the highest scores being achieved by WLP treated samples.&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> Andrei Iulian BORUZI Violeta NOUR Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-29 2019-05-29 47 3 10.15835/nbha47311458 Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba https://www.notulaebotanicae.ro/index.php/nbha/article/view/11428 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><em>Ginkgo biloba</em> is a unique tree in China with medicinally and phylogenetically important characteristics. Terpene trilactones (TTL) is a key active pharmaceutical ingredient in <em>Ginkgo</em>, so the content of TTL in <em>Ginkgo</em> has become one of the important indices for evaluating quality of the medicinal materials. By transcriptome sequencing on samples treated by chlormequat, ultraviolet (UV) and drought, totally 59820 contigs and 37564 unigenes were obtained. Furthermore, 18234 unigenes were annotated through COG, KEGG and GO analysis. There were 78 AP2/ERF transcription factors, 23 factors of up-regulation and 66 factors of down-regulation that were related with synthetic pathway of TTL in <em>Ginkgo</em>. Phylogenetic tree clustering analysis indicated that there were 42 AP2s could be clustered into ERF, DREB and RVA subfamilies. EMSA analysis demonstrated that GbERF13, GbERF25 and GbERF27 could bind with regulatory elements, such as E-box, in the upstream of GbMECPs promoter. Expression analysis showed that the expression level of GbERF25 was the highest in root, and GbERF25 and GbERF27 were expressed in relatively high transcription levels in leaf and other tissues. The results of qRT-PCR indicated that CCC treatment could significantly improve expression levels of ERF25 and ERF27, and UV and drought could induce transcription levels of ERF13 and ERF25, respectively. The results implied that ERF25 and ERF27 might involve in the induction and regulation of CCC treatment on synthesis of bilobalide in <em>G. biloba</em>. ERF13 might participate in the regulation of bilobalide synthesis induced by UV, and EFR25 might involve in the regulation of the synthesis induced by drought. During annual cycle of expression, the transcription levels of ERF13, ERF25 and ERF27 had significantly positive correlation with diterpene level with correlation coefficient 0.975. It implied that these transcription factors mainly acted on the MEP pathway that regulated synthesis of bilobalide. The aim of the research was to indicate the mechanism of environment or cultivation measure regulating target gene of TTL metabolic pathway by AP2/ERF, and establish metabolic network of AP2/ERF regulating TTL synthesis.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> Linling LI Honghui YUAN Sanxing ZHA Jie YU Xian XIAO Kun DENG Shuiyuan CHENG Hua CHENG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-29 2019-05-29 47 3 10.15835/nbha47311428 Nutritional Value of Garden Dill (Anethum graveolens L.), Depending on Genotype https://www.notulaebotanicae.ro/index.php/nbha/article/view/11395 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">The yield, chemical composition and antioxidant activity of eight genotypes (‘Krezus’, ‘Turkus’, ‘Kozak’, ‘Szmaragd’, ‘Lukullus’, ‘Herkules’, ‘Ambrozja’, ‘Moravan’) of garden dill (<em>Anethum graveolens</em> L.) biomass was estimated. Field experiments were conducted in 2011-2013 in Poland. The crop lasted 44 days and plants were collected at the 4-5 true leaf stage (a bunch harvest). The highest marketable yield was obtained for ‘Krezus’ and ‘Szmaragd’ cultivars (1.18 kg·m<sup>-2</sup>), while the lowest for ‘Herkules’ (0.53 kg·m<sup>-2</sup>). The dry matter of leaves ranged from 15.17% (‘Ambrozja’) to 19.27% (‘Krezus’). The study also proved that the genotype influenced the content of chlorophylls <em>a</em>+<em>b</em>, e.g. 1.10 g·kg<sup>-1</sup> (‘Moravan’) and 0.78 g·kg<sup>-1 (</sup>‘Lukullus’), respectively carotenoids: 21.43 mg·100 g<sup>-1</sup> (‘Moravan’) and 11.78 mg·100 g<sup>-1</sup> (‘Szmaragd’). The content of nitrates (371.48 mg·kg<sup>-1</sup> ‘Lukullus’ - 110 mg·kg<sup>-1</sup> ‘Szmaragd’), K (11.30% ‘Moravan’ - 5.37% ‘Kozak’), Ca (2.06% ‘Turkus’ - 1.77% ‘Kozak’), and oils (99.13% ‘Lukullus’ - 93.82% ‘Ambrozja’) was also varied. In most cases the content of Mg, P, sugars and ascorbic acid was on similar level. The antioxidant activity was significantly different between tested groups (12.22 µM·g<sup>-1</sup> ‘Turkus’ - 6.27 µM·g<sup>-1</sup> ‘Krezus’). The presented research proved that the genotype of garden dill affects yield and chemical composition of plants.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Anita BIESIADA Kamil KĘDRA Katarzyna GODLEWSKA Antoni SZUMNY Agnieszka NAWIRSKA-OLSZAŃSKA Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-31 2019-05-31 47 3 10.15835/nbha47311395 High-Density Kinetic Analysis of the Metabolomic and Transcriptomic Response of Ginkgo biloba Flavonoids Biosynthesis to Selenium Treatments https://www.notulaebotanicae.ro/index.php/nbha/article/view/11477 <p style="text-align: justify;">As one of the rare and precious wood species since the ancient times, <em>Gingko</em> is also known as “living fossil”, which is a special plant resource of China. <em>Gingko </em>leaves, containing rich flavonoids, are valued with great medicinal significances. This paper treated <em>Ginkgo</em> seedlings by exogenous Sodium selenite (SS) in two ways: Foliage dressing (FD) and Root application (RA). Then transcriptome sequencing and metabolome test are performed. Results show that external SS has significant influence on the related gene expression level of flavonoids synthesis ways of <em>Gingko</em>, the FD can significantly induce gene expressions as CHS, FLS, FOMT, PAL, MYB1 and MYB2, and RA can significantly induce gene expressions as FOMT, MYB1 and MYB2. Compared with the control group, FA selenium application can help to accumulation of flavonoids, flavonols, flavonoids-C and isoflavones, especially quercetin and kaempferol that had a remarkable increase. This proved that a proper concentration of inorganic SS could promote the synthesis and accumulation of flavonoids in <em>Gingko</em>. qRT-PCR analysis also depicts that leaves treatment of sodium selenite can remarkably enhance the gene expression of CHS, FLS, FOMT and PAL, and RA selenium application can induce the gene expression of FLS and FOMT, but restrain the gene expression of CHS and PAL. Through the ways of FD and RA selenium application, this paper basically studied the regulatory effect of SS on ginkgo flavonoids synthesis and has laid a theoretical basis to improve flavonoids content in <em>Ginkgo</em> leaves through cultivation control means.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;">*********</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;">*********</p> Linling LI Jie YU Honghui YUAN Sanxing ZHA Kun DENG Xian XIAO Yanyan LUO Shuiyuan CHENG Hua CHENG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-06-06 2019-06-06 47 3 10.15835/nbha47311477 Interactive Effects of Plant Growth-Promoting Rhizobacteria and Phosphates Sources on Growth and Phosphorus Nutrition of Soybean under Moderate Drought https://www.notulaebotanicae.ro/index.php/nbha/article/view/11371 <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">The growth of legume plants is usually improved by the rhizobacteria inoculation under low phosphorus (P) and alleviation of P nutrition plays important role in plant drought stress response. The aim of this study was to assess the comparative efficacy of two plant growth promoting rhizobacteria namely <em>Burkholderia cepacia </em>B36 and<em> Enterobacter radicincitans </em>D5/23T combined with two sources of phosphates in soybean (<em>Glycine max</em> L.) under low water supply. Plants were grown under P soluble versus insoluble P fertilization for comparing the effects of soybean inoculation on growth, uptake and use efficiency of phosphorus under moderate drought stress. At the beginning of flowering, half of plants was subjected to low water supply (35% water holding capacity, WHC) for 12 days while control plants were well watered - 70% WHC. The plants were harvested at the end of drought and physiological traits and P contents were analyzed. The inoculation treatments showed better plant growth and nutrient uptake when compared to uninoculated control. The application of the <em>Burkholderia cepacia</em> was more efficiently in terms plant growth than <em>E. radicincitans </em>especially under insoluble phosphates. Phosphorus concentrations of shoots and roots increased with both bacterial strains. The bacterial inoculation has much better stimulatory effect on nutrient uptake by soybean fertilized with insoluble phosphates. Study findings indicate that the combined application of PGPR (<em>Burkholderia cepacia </em>B36) and P amendments has the potential to improve P nutrition and growth of soybean cultivated on P-deficient soil under well-watered as well as moderate drought condition.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Vladimir Ion ROTARU Luxita RISNOVEANU Copyright (c) 2019-05-30 2019-05-30 47 3 10.15835/nbha47311371 Physicochemical Quality, Antioxidant Capacity and Nutritional Value in Tuberous Roots of Some Wild Dahlia Species https://www.notulaebotanicae.ro/index.php/nbha/article/view/11552 <p style="text-align: justify;">The aim of this research was to evaluate the physicochemical quality, antioxidant capacity and nutritional value in tuberous roots of some wild dahlia species. The experiment was carried out in the Department of Plant Science of the Autonomous University Chapingo, Mexico. Plants were established in a randomized complete block design with five replications. The total soluble solids (TSS), titratable acidity (TA), vitamin C (VC), total phenols (TP), antioxidant capacity (AC), inulin and its proximate composition were evaluated. Among the materials analyzed, the most outstanding wild species were <em>Dahlia campanulata</em>, <em>D. coccinea</em> and <em>D. brevis</em>, where <em>D</em>. <em>campanulata</em> stood out for its concentration of VC (0.05 mg 100 g<sup>-1</sup>), AC (1.88 mg VCEAC g<sup>-1</sup>), inulin, DM and TC (72.25, 24.38 and 88.37%, respectively), however, the inulin content was similar to <em>D</em>. <em>coccinea</em> (66.17%), which was also outstanding with respect to the content of TP (1.74 mg GA g<sup>-1</sup>). Likewise, <em>D</em>. <em>brevis</em> presented the highest values of RFi (5.49%) and A (78.42%). According to our results, the tuberous roots of wild dahlia species can be used as food, as well as being a source of selection of traits of nutraceutic interest for genetic improvement.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="text-align: justify;">&nbsp;</p> Esteban A. RIVERA-ESPEJEL Oscar CRUZ-ALVAREZ José M. MEJÍA-MUÑOZ María R. GARCÍA-MATEOS María T.B. COLINAS-LEÓN María Teresa MARTÍNEZ-DAMIÁN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-31 2019-05-31 47 3 10.15835/nbha47311552 Comparison of the Chemical Composition of Essential Oils Isolated from Two Thyme (Thymus vulgaris L.) Cultivars https://www.notulaebotanicae.ro/index.php/nbha/article/view/11451 <p style="text-align: justify;">In the studies conducted in 2016-2017, the chemical composition of the essential oils isolated from the herb of two thyme (<em>Thymus vulgaris </em>L.) cultivars was investigated by gas chromatography-mass spectrometry (GC-MS). The GC-MS analyses of the volatile oils of <em>T. vulgaris</em> L. cv<em>. </em>‘English Winter’ and cv<em>. </em>‘Summer Thyme de Provence’ led to the identification of 80 and 73 constituents, respectively. The main components found in the oil of ‘English Winter’ cultivar were thymol (44.97 and 38.06% in 2016 and 2017, respectively), p-cymene (7.61 and 10.34%), γ-terpinene (7.08 and 6.66%) and carvacrol (5.11 and 8.27%). Similarly, thymol (36.82 and 37.32%), p-cymene (15.73 and 11.20%), γ-terpinene (5.34 and 11.09%) and carvacrol (6.50 and 5.35%) dominated in the essential oil of ‘Summer Thyme de Provence’ cultivar. However, the higher content of thymol was noted for cultivar ‘English Winter’, while cultivar ‘Summer Thyme de Provence’ was richer in p-cymene.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Aneta WESOLOWSKA Dorota JADCZAK Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-31 2019-05-31 47 3 10.15835/nbha47311451 The Impact of Ammonium to Nitrate Ratio on the Growth and Nutritional Status of Kale https://www.notulaebotanicae.ro/index.php/nbha/article/view/11466 <p style="text-align: justify;">The effect of four NH<sub>4</sub>:NO<sub>3</sub> ratios in the nutrient solution (0:100; 25:75; 50:50 and 75:25) on growth and nutrient concentrations of four kale (<em>Brassica oleraceae</em> L. var. <em>acephala</em>) hybrids: ‘CN KAL 1029’, ‘Redbor’, ‘Winnetou’, ‘Reflex’ and&nbsp; one indigenous cultivar: ‘Ntopia Mytilinis’ was investigated. In the first four weeks of cultivation none of the NH<sub>4</sub>/NO<sub>3</sub> ratios applied induced adverse effects on most growth characteristics determined whereas plants grown with 75% NH<sub>4</sub>-N accumulated higher leaf N, P, K, Fe, Mn, Zn and Cu concentrations. After an eight week period, plants under 75% NH<sub>4</sub>-N showed significant reductions in many growth parameters suggesting a probable preference of kale plants grown for a prolonged period towards either a complete exclusion of NH<sub>4 </sub>from the nutrient solution or a 25:75 or a 50:50 NH<sub>4</sub>/NO<sub>3</sub> ratio. Among the genotypes tested, ‘Ntopia Mytilinis’ produced the greatest yield with the lowest leaf nitrate accumulation.&nbsp;</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Anna ASSIMAKOPOULOU Ioannis SALMAS Nikolaos KOUNAVIS Alexandros I. BASTAS Vasiliki MICHOPOULOU Evaggelos MICHAIL Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-31 2019-05-31 47 3 10.15835/nbha47311466 The Red Beech at the “Montagna di Torricchio”, Marche Region, Central Italy https://www.notulaebotanicae.ro/index.php/nbha/article/view/11583 <p style="text-align: justify;">For many centuries, red beech [(<em>Fagus sylvatica</em> L. var. <em>purpurea</em> (Ait.) Schneid.] was known as a botanical abnormality in botanical gardens and arboretums. Widespread artificially, red beech trees have been exploited for cultural, landscaping and botanical purposes, showing a wide horticultural, silvicultural and arboricultural interest. Over time, they may have escaped from cultivations or have been incorporated into forests by secondary successions, showing an important ecological and forestry interest. Red beeches represent a complex of phenotypes described under systematic critical taxa, showing an important floristic and taxonomic interest. Samples of red beech were found in nature, in central Adriatic Italy, in a fully protected area, namely the “Riserva Naturale Statale Montagna di Torricchio”. In order to analyse the origin of this odd shape beech variation, an interdisciplinary study was carried out integrating geography, ecology and forestry, analysing the bibliography, archival data, leaf traits, potential vegetation and the issues concerning the human-ecology relation. The results we obtained showed no evidence of artificial or accidental introduction for these specimens, suggesting treating them as a native and natural mutation in the beech forest secondary succession. The study of the natural beech forms variation could foster in some way research activities and land management actions on this issue; with some outlook on biodiversity studies, environmental policies, horticulture, forestry and ecological importance. The results allow us to propose a reflexion on the native/non-native status, and about the systematic position of beech forms; fostering reflections about the taxonomical position of the red beech and proposing the variety rank for the red beech specimens. The presence of these specimens in the study area, allows us to make some reflections in order to be more careful in negative considerations about exotic (or presumed) taxa, taking into account the repercussions on environment and biodiversity management. These red beeches can be used to strengthen the genetic pool of existing specimens that are actually used, and vegetatively propagated in nurseries.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;">*********</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;">*********</p> Kevin CIANFAGLIONE Franco PEDROTTI Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-06-05 2019-06-05 47 3 10.15835/nbha47311583 Validation of Gender Specific CAPS Marker in Turkish Fig (Ficus carica L.) Collection and F1 Progenies https://www.notulaebotanicae.ro/index.php/nbha/article/view/11499 <p style="text-align: justify;">In most dioecious plants, distinguishing male and female progenies is not possible until flowering or fruiting stage. The fig (<em>Ficus carica</em> L.) is such a plant where distinguishing male and female plants at the seedling stage can accelerate fig-breeding programs. An orthologue of <em>RAN1</em> loci was reported to be associated with sex determination in fig (Mori <em>et al</em>., 2017). The objective of this study is to validate this locus on Turkish fig germplasm collection and F1 population obtained from a cross between female genotypes ‘Bursa Siyahi’ and male genotype ‘Ak Ilek’. A total of 144 genotypes from germplasm collection and 115 F1 individuals were tested with CAPS (cleaved amplified polymorphic sequences) marker following the Mori <em>et al</em>. (2017). The loci produced a 315bp amplification product from all genotypes. <em>PciI </em>digestion of PCR products resulted in 100% concordance between phenotypes and molecular tests. On the other hand, <em>HpyCH4IV</em> enzyme digestion of 8 female genotypes resulted in false negatives among the tested materials. Therefore, despite overall results show that the locus is suitable for gender selection of plants at the seedling stage in the breeding programs, care should be taken when <em>HpyCH4IV </em>enzyme is to be employed for CAPS assay.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Hatice IKTEN Yeliz YILMAZ Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-18 2019-06-18 47 3 10.15835/nbha47311499 Growth, Photosynthetic Pigments, Phenolic, Glucosinolates Content and Antioxidant Capacity of Broccoli Sprouts in Response to Nanoselenium Particles Supply https://www.notulaebotanicae.ro/index.php/nbha/article/view/11490 <p style="text-align: justify;">Improving the nutritional quality of plants has emerged from the fact that macro- and micro- nutrients are limited in various agricultural areas. The aim of our study was the biofortification of broccoli sprouts with selenium nanoparticles (NSePs) and evaluation of growth parameters, assimilator pigments content, total phenols, glucosinolates content along with antioxidant capacity, in order to boost value added output, such as improved nutrition and food functionality. NSePs were prepared by reduction of NaHSeO<sub>3</sub> using glucose as reducing agent, and characterized from structural and morphological point of view. The growth of broccoli seedlings was dependent on NSePs concentration. The treatment with 10 and 50 ppm NSePs caused a slight increase in total biomass, by contrast with 100 ppm treatment. Chlorophyll content, total carotenoid and total phenols content was not affected by the treatment of broccoli sprouts with different concentrations of NSePs. Instead, the content of individual glucosinolates varied between the samples, depending on the levels of NSePs. The highest antioxidant capacity was obtained for 100 ppm NSePs concentration. The effective uptake of NSePs was further demonstrated by FTIR spectroscopy and Hyperspectral Microscopy. NSePs did not induce any toxicity on broccoli sprouts. Moreover, broccoli supply with NSePs may target higher nutritional impact and health benefits.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> Simona Ioana VICAS Simona CAVALU Vasile LASLO Mariana TOCAI Traian O. COSTEA Liviu MOLDOVAN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-18 2019-06-18 47 3 10.15835/nbha47311490 Effect of Maturation Degree on the Fixed Oil Chemical Composition, Phenolic Compounds, Mineral Nutrients and Antioxidant Properties of Pistacia lentiscus L. Fruits https://www.notulaebotanicae.ro/index.php/nbha/article/view/11534 <p style="text-align: justify;">This study aimed to evaluate the maturity stage effects on the bioactive metabolites content, the antioxidant activity and color variation of Algerian <em>Pistacia lentiscus</em> L. fruit. For this propose, both red and black fruits of the selected species representing two different maturation stages immature and mature, respectively are chosen. Our experimental study was initiated by a GC/MS analysis in order to determinate the fixed oils chemical composition. An AAS for macroelements and ICP-MS for microelements was carried out to evaluate the mineral composition. Furthermore, phenolic, anthocyanins and total sugars compounds level were determinate. Also, a chromameter was used to detect the color changes of fruits powders. At last, antioxidant activities were evaluated by DPPH and FRAP assays. As a result, the fixed oil qualitative evaluation, revealed new molecules were synthesized in the mature fruit oil which gives it a higher quality than one of the immature stage, we also noted that the accumulation of the oils increases with the fruits maturation while the sugar content decreases significantly. In addition, the results obtained showed that the bioactive molecules were found to be significantly high at the mature stage and its affects positively the <em>P. lentiscus</em> antioxidant activity. High levels of minerals (Ca, Mg, Mn, Cu and Zn) observed in mature fruits ensure a high nutritional quality compared to immature ones.&nbsp; To conclude, the phytocomponents of <em>P. lentiscus</em> fruit are accumulated during maturation, which provides it with an important qualitative and quantitative value in terms of both curative and/ or nutritional fields.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Kaissa BOUDIEB Sabrina AIT SLIMANE-AIT KAKI Hayet AMELLAL-CHIBANE Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-18 2019-06-18 47 3 10.15835/nbha47311534 Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots https://www.notulaebotanicae.ro/index.php/nbha/article/view/11453 <p style="text-align: justify;">This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (<em>Lepidium sativum</em> L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p &lt; 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> Ibrahim DEMIR Burcu B. KENANOGLU Eren ÖZDEN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-18 2019-06-18 47 3 10.15835/nbha47311453 Foliar N:P Stoichiometry in Aralia elata Distributed on Different Slope Degrees https://www.notulaebotanicae.ro/index.php/nbha/article/view/11390 <p style="text-align: justify;">Plant nitrogen (N) to phosphorus (P) stoichiometry is of essentially ecological meaning to non-wood forest production (NWFP) plant community in the temperate forest ecosystem. In this study, natural <em>Aralia elata</em> (Miq.) Seem. communities in montane areas of southern Heilongjiang Province, Northeast China were investigated for plant density, vegetative growth, and soil and leaf parameters on slopes in 5°, 9°, and 14° degrees. We found that individual height was greater in sites on 5°- (1.6 m) and 9°-degree slopes (1.9 m) than on the 14°-degree slope (0.8 m), but soil available P content was highest on the steepest slope (5.5, 4.0, and 16 mg kg<sup>-1</sup>, respectively). The foliar N:P ratio ranged 6–13. Nitrogen availability tended to promote community density and individual growth; while P availability tended to depress density but promote foliar biomass accumulation. By the diagnosis of plant nutritional monogram, the better growth and high foliar N:P ratio of about 13:1 in <em>A. elata</em> individuals on 9°-degree slope were generated by both deficiency-driving N uptake and excessive P depletion. We recommend the 9°-degree slope to develop <em>A. elata</em> community which can be fed by higher N availability if higher density was achieved.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Hongxu WEI Hengtian ZHAO Xin CHEN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-19 2019-06-19 47 3 10.15835/nbha47311390 Effect of Different DNA Demethylating Agents on In vitro Cultures of Peach Rootstock GF 677 https://www.notulaebotanicae.ro/index.php/nbha/article/view/11373 <p style="text-align: justify;">The appearance of somaclonal variability induced by <em>in vitro</em> cultivation is relatively frequent and, in some cases, provides a valuable source of new phenotypes suitable for crop improvement. Numerous studies have confirmed that these changes can be explained by alterations of DNA methylation. Interestingly, a group of chemical compounds termed ‘demethylating agents’ (DMT agents) enable artificial changes to be made in the DNA methylation state. Thus, these agents are theoretically able to induce new phenotypes or more favourable properties. The objective of the present study was to verify suitable conditions for the application of different DMT agents within <em>in vitro</em> protocols for micropropagation using the stone fruit rootstock GF 677 as an example. The impact of these agents on the properties of plant regenerants was evaluated, and their DNA methylation state was controlled by using an AFLP protocol based on a restriction endonuclease that differed in its sensitivity to methylated cytosines. Moreover, the effect of newly synthesised derivates was compared with that of conventional compounds with a well-documented DNA-demethylating impact. Based on the results, the suitable concentration for treatment by a DMT agent was established as approximately 50 µM. Promising results were generated using a combination of DMT agents with different mechanisms of action, such as azacytidine and dihydroxypropyladenine; under these conditions, probable synergy between methyltransferase interception by the cytosine analogue and interruption of methyl group donation by dihydroxypropyladenine significantly changed the DNA methylation state of treated plants. Regarding newly synthesised compounds, the 5,6-dihydro-5-azacytosine nucleoside showed the most promising results, which can likely be explained by its higher stability in the media used for <em>in vitro</em> cultivation.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> Miroslav BARÁNEK Miroslav OTMAR Marcela KREČMEROVÁ Aleš EICHMEIER Jana MOUDRÁ Zuzana MYNARZOVÁ Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-06-18 2019-06-18 47 3 10.15835/nbha47311373 Characterization and Tissue-specific Expression of bHLH Genes in Dimocarpus longan https://www.notulaebotanicae.ro/index.php/nbha/article/view/11531 <p style="text-align: justify;">In plants, the basic helix-loop-helix (<em>bHLH</em>) transcription factors (TFs) play pivotal roles in many biological processes including growth, stress response, and secondary metabolite synthesis. To date, many <em>bHLH</em> genes have been identified and characterized in diverse plant species. However, little is known regarding the <em>bHLH</em> genes in <em>Dimocarpus longan</em> Lour. (<em>D. longan</em>). Based on RNA-seq data, we identified 42 putative <em>bHLH</em> genes from <em>D. longan</em> and determined their putative functions using the NCBI Conserved Domain Search Tool and Pfam databases. The physicochemical properties, phylogenetic relationships, conserved motifs, gene ontology (GO) annotations, protein-protein interactions, and tissue-specific expression patterns of these <em>bHLH</em> genes were systematically explored. In total, ten motifs were found in DlbHLH proteins using MEME, among which two were highly conserved. Phylogenetic tree analysis found that DlbHLH proteins can be divided into nine groups, with group 2 being the largest. GO annotation results showed that the <em>DlHLH</em> genes were involved in various molecular functions. RNA-seq and qRT-PCR results revealed important differences in the expression patterns of 17 of the <em>DlbHLH</em> genes. In particular, <em>DlbHLH-9</em>, <em>DlbHLH-19, DlbHLH-25, DlbHLH-26,</em> and <em>DlbHLH-35 </em>were found to show significantly different expression patterns in root and leaf tissues. The results of this study will further enrich our knowledge regarding <em>bHLH</em> transcription factor genes and lay a foundation for enhancing the production of active secondary metabolites by genetic engineering in <em>D. longan</em>.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Wei ZHENG Xueming DONG Xuefei YU Qiuying ZHANG Ning CHEN Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-01 2019-07-01 47 3 10.15835/nbha47311531 Cytological Investigation in Two Mutants of the Hybrid Grapevine ‘Niagara’ https://www.notulaebotanicae.ro/index.php/nbha/article/view/11470 <p style="text-align: justify;">‘Niagara’ is a hybrid table grape (<em>V. vinifera</em> × <em>V. labrusca</em>). Several spontaneous somatic mutants from this grapevine have already been described. However, cytological information about these mutants is scarce. Therefore, studies in the microsporogenesis have been carried out in two of these mutants, a seedless and a large berry mutant (giant mutant). A low percentage of abnormalities such as precocious chromosome segregation, bivalent not aligned, laggard and stickiness chromosomes were seen in both mutants.&nbsp; A merging process of microspores was observed in several tetrads in the seedless mutant: the microspore came together by large cytoplasmic bridges giving rise to a large and amorphous single-celled tetrad, which eventually collapsed. The giant mutant showed 10.29% of tetrads with microcytes. However, these tetrad abnormalities did not hinder pollen viability. Significant differences were seen concerning stomata and pollen grain sizes, stomata frequency and anther number per flower between the mutants. The chromosome count at diakinesis (<em>n</em>=19) and in diploid tapetal cells (2<em>n</em>=38) clarified the fact that the seedless somatic mutation was not associated with any ploidy event. Conversely, the giant mutant showed <em>n</em>=38 at diakinesis and 2<em>n</em>=76 in diploid tapetal cells, pointing to a case of spontaneous chromosome duplication. Since these abnormalities did not disturb the percentage of pollen viability very much, in both mutants (higher than 86%), the seedless one may be used as a male parent in crossing, regarding the desirable seedlessness character, while the mutant giant may be employed as female parent for the obtainment of seedless triploid plants.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p style="text-align: justify;"><strong>*********</strong></p> Neiva Izabel PIEROZZI Mara Fernandes MOURA Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-03 2019-07-03 47 3 10.15835/nbha47311470 The Influence of Aqueous Extracts from Stellaria media L. on the Growth of Zea mays L. Cultivars https://www.notulaebotanicae.ro/index.php/nbha/article/view/11597 <p style="text-align: justify;">Plants introduce chemicals into the environment that can be toxic, both for themselves and for other species. Weed infestation of crops is a competition for environmental resources, but at the same time is a source of chemical substances released into the soil, often reducing yield potential. The paper attempts to investigate the allelopathic interaction of aquatic extracts from dry shoots of chickweed <em>Stellaria media</em> L. on germination and development of maize <em>Zea mays</em> L. seedlings of ‘San’, ‘Kidemos’ and ‘DKC 3441’ cultivars. Along with the increase in the concentration of allelopathic substances, a decrease in the germination activity of all tested maize cultivars was observed. Germination percentage as like percentage of control was the highest for seeds germinating on substrates with 1% chickweed extracts and the lowest for seeds watered with 5% extracts. Biometric measurements of seedlings showed that the most sensitive cultivar of maize was ‘DKC 3441’, and the resistant cultivar ‘San’. The relative water content was the highest for seedlings grown on the 5% extracts. Significant differences in the amount of fresh and dry masses of maize seedlings were demonstrated at the highest concentrations of extracts compared to the control. A significant increase in electrolyte leakage compared to the control sample was found in the ‘Kidemos’ cultivar. The lowest differences in the destabilization of the ionic transport through cell membranes for the ‘DKC 3441’ cultivar were demonstrated.</p> <p>&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"><span style="background: white;">*********</span></span></strong></p> <p style="margin: 15pt 0cm; line-height: 18.75pt; background: white none repeat scroll 0% 0%; box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; word-spacing: 0px; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;"><span style="background: white;">In press - Online First</span></strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"><span style="background: white;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;"><u>DOI link will become active after the article will be included in the complete issue.</u></span></span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"><span style="background: white;">*********</span></span></strong></p> Peiman ZANDI Katarzyna MOŻDŻEŃ Beata BARABASZ-KRASNY Joanna PUŁA Alina STACHURSKA-SWAKOŃ Yaosheng WANG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-06 2019-07-06 47 3 10.15835/nbha47311597 The eggplant yield and fruit composition as affected by genetic factor and biostimulant application https://www.notulaebotanicae.ro/index.php/nbha/article/view/11468 <p style="text-align: justify;">Consumer demands regarding eggplant fruits are focused on perfect shape, color, and taste but nutritional and bioactive quality has also become important with rising awareness of food related health. On the other hand, producers value modern cultivars’ earliness, high-yielding, stress-tolerance, and high overall fruit quality. We attempt to link these two standpoints through combining modern hybrids cultivated in the open field of temperate climatic zone with biostimulant application to increase the dynamics of yielding and the quality of fruits. The aim of our research was the determination of the genotypic-dependent response of eggplant (<em>Solanum melongena</em> L.) to <em>Ascophyllum nodosum</em> standardized extract (Göemar BM-86) application in field conditions of Poland, with respect to the most important characteristics linked to yield quality and plant stress status. The early and total yield of investigated hybrids was affected by biostimulant application, and this dependence was confirmed statistically for most of the treatments. The increase of ‘Epic’ F<sub>1</sub> and ‘Flavine’ F<sub>1</sub> yield potential through Göemar BM-86 application was linked to higher fruit number harvested from the plants, while ‘WA 6020’ F<sub>1</sub> led to greater fruit weight. Investigated hybrids responded differently to biostimulant treatment with respect to dietary and pro-health value of fruits. Generally, the content of soluble sugar, anthocyanins, and mineral elements as well as was antioxidant activity of fruits were positively affected by biostimulant application. This tendency was statistically confirmed for several experimental treatments. Presented results give a new perspective on seaweed-based biostimulants as elicitors of crop’s self defense mechanisms as well as modulators of fruit setting, productivity and bioactive compounds accumulation in eggplant.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> Alicja POHL Aneta GRABOWSKA Andrzej KALISZ Agnieszka SEKARA Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-06 2019-07-06 47 3 10.15835/nbha47311468 High frequency 2n pollen formation in black poplar (Populus nigra L.) induced by colchicine https://www.notulaebotanicae.ro/index.php/nbha/article/view/11521 <p style="text-align: justify;"><em>Populus nigra </em>L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent <em>Populus nigra </em>clones are identified as triploids potentially obtaining by hybridization of <em>2n</em> pollen and normal oogamete. This study revealed the cytological mechanism of <em>2n</em> pollen formation in <em>Populus nigra </em>L. for the first time and concluded the best treated combination of colchicine treatment, which obtained <em>2n</em> pollen with the highest rate reaching at 87.11% (even 100% <em>2n</em> pollen in some floral buds) which made the polyploid hybridization utilizing <em>2n</em> pollen in section<em> Aigeiros</em> possible.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue.</u></p> <p><strong>*********</strong></p> Wenting XU Yan ZHANG Liqin GUO Jian ZHAO Zunzheng WEI Jinfeng ZHANG Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-10 2019-07-10 47 3 10.15835/nbha47311521 Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions https://www.notulaebotanicae.ro/index.php/nbha/article/view/11488 <p style="text-align: justify;">The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term <em>in vitro</em> cultured fraser photinia microshoots, on seed and 7-day old seedling stages of <em>Arabidopsis thaliana</em>. Seeds and <em>in vitro</em>-germinated seedling<em>s</em> were inoculated with 10<sup>7</sup> CFU/mL and 10<sup>8</sup> CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll <em>a, b</em> and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of <em>virD1</em> gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for <em>A. thaliana</em> when optimum conditions and concentrations are used. Moreover, presence of <em>virD1</em> gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.</p> <p style="text-align: justify;">&nbsp;</p> <p><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First. </strong>Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<u>DOI link will become active after the article will be included in the complete issue</u>.</p> <p><strong>*********</strong></p> Maria BATOOL Özlem AKKAYA Mine GÜL ŞEKER Yelda ÖZDEN ÇİFTÇİ Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://creativecommons.org/licenses/by/4.0 2019-07-19 2019-07-19 47 3 10.15835/nbha47311488