https://www.notulaebotanicae.ro/index.php/nbha/issue/feed Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019-05-21T14:41:33+00:00 Radu E. SESTRAS rsestras@notulae.ro Open Journal Systems <p style="text-align: justify;"><strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> is a peer-reviewed&nbsp;<strong>quarterly (beginning in 2019)</strong> journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (<strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca</strong> or JCR abbrev. title <strong>Not Bot Horti Agrobo</strong>) are mentioned.</p> <p style="text-align: justify;"><strong>Editor-in-Chief</strong></p> <ul> <li class="show"><a href="https://www.sestras.ro/">Radu E. SESTRAS</a></li> </ul> <p><strong>Advisory Editors</strong></p> <ul> <li class="show">Cornel CATOI</li> <li class="show">Viorel MITRE</li> <li class="show">Doru C. PAMFIL</li> </ul> <p><a href="/index.php/nbha/about/editorialTeam" target="_blank" rel="noopener"><strong>Editorial Board</strong></a></p> <p><strong>Journal Secretary</strong></p> <ul> <li class="show">Roxana L. STOIAN</li> <li class="show">Silviu L. MIC</li> </ul> <p>&nbsp;</p> <p>Print <strong>ISSN 0255-965X; </strong>Electronic <strong>ISSN 1842-4309</strong></p> <p><strong>License: &nbsp;</strong><strong> <a title="CC BY 4.0" href="https://creativecommons.org/licenses/by/4.0/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/by1.png" width="80" height="28"></a></strong><strong>&nbsp; <a title="About The Licenses" href="https://creativecommons.org/licenses/" target="_blank" rel="noopener"><img src="/public/site/images/silviu_mic/cc.logo_.png" width="118" height="28"></a></strong></p> <p>&nbsp;</p> <p><strong>Open Access Journal:</strong> Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.</p> <p>&nbsp;</p> <ul> <li class="show">See information on <strong>‘</strong><strong>Policies’</strong> (<a href="/index.php/nbha/focus-scope">Focus and Scope</a>; <a href="/index.php/nbha/about/editorialPolicies#peerReviewProcess">Peer Review Process</a>; <a href="/index.php/nbha/about/editorialPolicies#publicationFrequency">Publication Frequency</a>; <a href="/index.php/nbha/about/editorialPolicies#openAccessPolicy">Open Access Policy</a>; <a href="/index.php/nbha/about/editorialPolicies#custom-0">Publication Ethics and Publication Malpractice Policy</a> etc.); <strong>‘</strong><strong>Submissions’</strong> (<a href="/index.php/nbha/about/submissions#onlineSubmissions">Online Submissions</a>; <a href="/index.php/nbha/about/submissions#authorGuidelines">Author Guidelines</a>; <a href="/index.php/nbha/about/submissions#copyrightNotice">Copyright Notice</a>; <a href="/index.php/nbha/about/submissions#privacyStatement">Privacy Statement</a>; <a href="/index.php/nbha/about/submissions#authorFees">Payment</a>&nbsp;etc.).</li> </ul> <p>&nbsp;</p> <ul> <li class="show"><strong>June 1, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in <strong><span style="color: #297439;"><a class="cta-secondary" style="color: #297439;" title="CiteScore 2018 value" href="https://journalmetrics.scopus.com/" target="_blank" rel="noopener noreferrer">CiteScore rank (Scopus – Elsevier)</a></span></strong><strong> <span style="color: #297439;"><a href="https://www.scopus.com/sourceid/17700156420?origin=sbrowse">#34/72 in Horticulture</a> </span></strong></li> <li class="show"><strong>June 26, 2018: </strong>Notulae Botanicae Horti Agrobotanici Cluj-Napoca in<strong> <span style="color: #297439;"><a href="https://clarivate.com/blog/science-research-connect/the-2018-jcr-release-is-here/"><span class="cta-secondary" style="color: #297439;">ISI Clarivate Analytics</span> 183/222 in Plant Sciences</a></span></strong><strong><span style="color: #297439;">&nbsp;</span></strong></li> </ul> <p align="center">&nbsp;</p> <p align="center"><strong>Published by AcademicPres on behalf of:</strong></p> <p align="center">University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca</p> <p align="center">Romanian Academy of Agricultural and Forestry Sciences</p> <p align="center">Horticulture and Forestry Society from Transylvania</p> <p align="center">&nbsp;</p> <p class="functie"><strong>Abstracted and/or indexed:</strong></p> <ul> <li class="show">Clarivate Analytics, Master Journal List - SCIE, JCR, <a href="http://mjl.clarivate.com/">http://mjl.clarivate.com/</a>&nbsp;(IF 2017=0.648)</li> <li class="show">Scopus - Elsevier, <a href="https://www.scopus.com/sourceid/17700156420">https://www.scopus.com/sourceid/17700156420</a> (CiteScore 2017, metrics 2018=0.78)</li> <li class="show">ProQuest,&nbsp;<a href="http://www.proquest.com/">http://www.proquest.com/</a></li> <li class="show">Centre for Agriculture and Biosciences International (CABI),&nbsp;<a href="https://www.cabdirect.org/">https://www.cabdirect.org/</a></li> <li class="show">Chemical Abstracts Service (CAS),&nbsp;<a href="https://www.cas.org/">https://www.cas.org/</a></li> <li class="show">Russian Scientific and Technical Information Institute (VINITI),&nbsp;<a href="http://www.viniti.ru/">http://www.viniti.ru</a></li> <li class="show">Bielefeld Academic Search Engine (BASE),&nbsp;<a href="http://www.base-search.net/">http://www.base-search.net</a></li> <li class="show">Google Scholar,&nbsp;<a href="http://scholar.google.com/">http://scholar.google.com</a></li> <li class="show">British Library Direct (BLD),&nbsp;<a href="http://explore.bl.uk/">http://explore.bl.uk/</a></li> <li class="show">Mendeley,&nbsp;<a href="https://www.mendeley.com/research-papers/?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">https://www.mendeley.com</a></li> <li class="show">The European Library,&nbsp;<a href="http://www.theeuropeanlibrary.org/tel4/search?query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca">http://www.theeuropeanlibrary.org</a></li> <li class="show">Harvard Library,&nbsp;<a href="http://hollis.harvard.edu/primo_library/libweb/action/display.do?tabs=detailsTab&amp;ct=display&amp;fn=search&amp;doc=HVD_ALEPH011313468&amp;indx=1&amp;recIds=HVD_ALEPH011313468&amp;recIdxs=0&amp;elementId=0&amp;renderMode=poppedOut&amp;displayMode=full&amp;frbrVersion=&amp;frbg=&amp;&amp;vl(51615747UI0)=title&amp;dscnt=0&amp;vl(1UIStartWith0)=contains&amp;scp.scps=scope%3A%28HVD_FGDC%29%2Cscope%3A%28HVD%29%2Cscope%3A%28HVD_VIA%29&amp;tb=t&amp;mode=Basic&amp;vid=HVD&amp;tab=books&amp;srt=rank&amp;vl(394521272UI1)=all_items&amp;vl(freeText0)=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;dum=true&amp;dstmp=1510572056817">http://hollis.harvard.edu</a></li> <li class="show">Tianjin University of Finance and Economics (TJUFE),&nbsp;<a href="http://en.tjufe.findplus.cn/?h=search_list&amp;query=Notulae%20Botanicae%20Horti%20Agrobotanici%20Cluj-Napoca&amp;action[addexpander][]=fulltext">http://en.tjufe.findplus.cn</a></li> <li class="show">Open Archives Initiative Protocol for Metadata Harvesting (OAIster),&nbsp;<a href="http://www.oaister.org/">http://www.oaister.org</a></li> <li class="show">China Educational Publications Import and Export Corporation (SOCOLAR),&nbsp;<a href="http://www.socolar.com/">http://www.socolar.com</a></li> <li class="show">Ulrich's Periodicals Directory,&nbsp;<a href="http://www.ulrichsweb.com/">http://www.ulrichsweb.com</a></li> <li class="show">Elton B Stephens COmpany (EBSCO),&nbsp;<a href="http://www.ebscohost.com/">http://www.ebscohost.com</a></li> <li class="show">Information Matrix for the Analysis of Journals (MIAR),&nbsp;<a href="http://miar.ub.edu/">http://miar.ub.edu/</a></li> </ul> <div>&nbsp;</div> <p align="center"><em>First published 1965</em></p> <p align="center"><em>Print ISSN 0255-965X, Electronic ISSN 1842-4309</em></p> <p class="subpunct" align="center"><strong><em><a href="/index.php/nbha">www.notulaebotanicae.ro</a></em></strong></p> https://www.notulaebotanicae.ro/index.php/nbha/article/view/11296 Ecology, Cultivation and Utilization of the Aromatic Greek Oregano (Origanum vulgare L.): A Review 2019-04-19T08:46:21+00:00 Elpiniki SKOUFOGIANNI eskoufog@uth.gr Alexandra D. SOLOMOU alexansolomou@gmail.com Nicholaos G. DANALATOS danal@uth.gr <p style="text-align: justify;">Medicinal and aromatic plants represent a stable part of the natural biodiversity legacy of many countries in the world. The present review focuses on oregano (<em>Origanum vulgare</em> L.; family Lamiaceae), an endemic herb in Greece that constitutes one of the best known aromatic and medicinal plants originating in the Mediterranean region. In particular, oregano is an evergreen, rich in natural compounds perennial plant that received increased attention in the last years for a wide range of uses. Oregano dry leaves and inflorescences in mixture are used as human and animal food that is extremely rich in antioxidative properties. Additionally, its essential oil is rich in carvacrol, thymol, c-terpinene and p-cymene, and is used for a number of medicinal purposes, e.g. for inhibiting microbial and fungal toxin production as well as for the well-known anti-inflammatory, analgesic, antiarthritic, antiallergic, anticarcinogenic, antidiabetic, cardioprotective, gastroprotective, hepatoprotective, and neuroprotective properties. Due to its perfect quality and high essential oil concentration, Greek oregano (<em>O. vulgare</em> ssp. <em>hirtum</em>) is regarded among the best in the world gaining in popularity in the global markets for food industry applications. Consequently, oregano might be considered as an important low-input, environmental friendly commodity for extensive cultivation in Greece. The present review summarizes on the origin, the morphology, the ecology and the utilization of this plant. Despite the extensive literature available on the use of oregano biomass and essential oil, only few reports exist concerning the cultivation of this plant. Therefore, the present review is additionally focused on the cultivation practices and the importance of cultivation and utilization of <em>Origanum vulgare</em> L. in Greece and generally in the Mediterranean region in the near future, as it constitutes a plant species with high medical, economic and environmental value.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> 2019-04-10T00:00:00+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11489 Some Biochemical Characteristics of Fruit Samples of Sambucus nigra Genotypes from Northeastern Turkey 2019-04-19T17:15:18+00:00 Tuncay KAN kantunca@gmail.com <p style="text-align: justify;">There are numerous wild edible fruit species in the world and they are important components of dietary utilization, local pharmacopoeia, cultural and religious practices of indigenous people. In this study, some important berry characteristics of seven seed propagated black elderberry (<em>Sambucus nigra)</em> genotypes sampled from Rize province, Turkey, were assessed. The genotypes exhibited a great diversity on most of the biochemical characteristics. Berries of genotypes showed moderate Vitamin C content ranged from 26 to 38 mg per 100 g fresh weight. Total phenolic and total anthocyanin content in fruits of the <em>S. nigra </em>genotypes has also high value ranged from 360 to 447 mg gallic acid equivalent (GAE) per 100 g fresh weight and 307 to 412 mg cyanidin-3-glucoside equivalent per 100 g fresh berry, respectively. Antioxidant activity was found between 5.28 and 7.11 mmol trolox equivalent per 100 g fresh weight basis. Citric acid and fructose determined as major organic acid and sugar for all genotypes. For its high vitamin C, total anthocyanin and total phenolic content and high antioxidant activity of fruits of wild grown <em>S. nigra</em> may have provide many health benefits and can be domesticated to establish orchards in Turkey.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> 2019-04-10T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11401 Influence of Selected Antibiotics on the Tomato Regeneration in In Vitro Cultures 2019-04-19T17:20:44+00:00 Aneta GERSZBERG angersz@biol.uni.lodz.pl Izabela GRZEGORCZYK-KAROLAK izabela.grzegorczyk@umed.lodz.pl <p style="text-align: justify;">Generally, antimicrobial agents are frequently used in micropropagation techniques to obtain free elite clones or after genetic transformation to select putative transformants. Their successful application minimizes bacterial contamination however; they may be phytotoxic and may diversely affect the regeneration ability in plant tissue cultures. The objective of the current study was to estimate the effects of four antibiotics i.e. ampicillin, carbenicillin, cefotaxime and kanamycin on morphogenesis of three Polish tomato cultivars, cultured on MS medium with phytohormones. In this experiment the ability of tomato cotyledon explants to regenerate entire plants via indirect organogenesis was tested. Among four antibiotics tested, kanamycin was most harmful for the explants. This antibiotic, even at low doses (10-20 mg/L), inhibited tomato morphogenesis. On the other hand, the current study revealed significant influence of different concentrations of ampicillin, carbenicillin, cefotaxime on the frequency of bud formation. While the addition of cefotaxime at low concentration (100-200 mg/L) stimulated the bud formation, its increasing concentration adversely affected the organogenesis of tomato. The results clearly pointed out that carbenicillin and ampicillin at low concentrations (100-400 mg/L) were not only non-toxic, but they promoted bud regeneration. The obtained results show the crucial role not only of use of efficient antibiotics, but also of their proper doses in obtaining successful transformation and regeneration of tomato.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> 2019-04-10T00:00:00+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11465 Phytochemical and Antioxidant Properties of Fresh Fruits and Some Traditional Products of Wild Grown Raspberry (Rubus idaeus L.) 2019-04-19T17:22:07+00:00 Bojana VELJKOVIĆ bojana.radulovic@yahoo.com Violeta JAKOVLJEVIĆ jakovljevicvioleta@gmail.com Milan STANKOVIĆ mstankovic@kg.ac.rs Zora DAJIĆ-STEVANOVIĆ dajic@agrif.bg.ac.rs <p style="text-align: justify;">The current study investigated and compared phytochemical and antioxidant activity of fresh fruit and some traditional products of <em>Rubus idaeus</em> grown in mountain region of Serbia. The total organic acid, total sugar content, total phenolics, flavonoids, tannins, anthocyanins and vitamin C were evaluated. The antioxidant activities were evaluated using two antioxidant systems 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The fresh fruit contained highest amount of vitamin C (46.62 mg AA g<sup>-1</sup>) and total organic acids (882.22 mg CA g<sup>-1</sup>). The sweet preserve had highest content of total phenolics (200.83 mg GA g<sup>-1</sup>), flavonoids (12.85 mg RU g<sup>-1</sup>) and tannins (39.11 mg g<sup>-1</sup>). The juice had the highest total anthocyanin content (107.22 µg mL<sup>-1</sup>) and total sugar content (25 °Brix). The best antioxidant activity in ABTS assay had juice (IC<sub>50 </sub>= 4.87 µmol TE g<sup>-1</sup>), followed by sweet preserve (IC<sub>50 </sub>= 5.14 µmol TE &nbsp;g<sup>-1</sup>), almost identical to standard gallic acid. In the DPPH free radical scavenging assay, sweet fruit preserve showed significant better antioxidant activity (IC<sub>50</sub> = 41.27 µg mL<sup>-1</sup>) compared to juice (IC<sub>50</sub> = 106.07 µg mL<sup>-1</sup>) and fresh fruit (IC<sub>50</sub> = 294.79 µg mL<sup>-1</sup>). Our results indicated promising perspectives for usage of <em>R. idaeus</em> fresh fruits and traditional products studied with considerable levels of vitamin C, bioactive compounds and antioxidant activity.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T00:00:00+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11487 Non-Destructive Leaf Area Estimation Model for Overall Growth Performances in Relation to Yield Attributes of Cassava (Manihot esculenta Cranz) under Water Deficit Conditions 2019-04-19T17:23:33+00:00 Piyanan PIPATSITEE piyanan.pip@biotec.or.th Apisit EIUMNOH apisit.eiu@biotec.or.th Patchara PRASEARTKUL patchara.pra@biotec.or.th Navavit PONGANAN navavit.pon@biotec.or.th Kanyarat TAOTA kanyarat.tao@ncr.nstda.or.th Sumaid KONGPUGDEE sumaid.kon@ncr.nstda.or.th Kampol SAKULLEERUNGROJ kampol.sak@ncr.nstda.or.th Suriyan CHA-UM suriyanc7@gmail.com <p style="text-align: justify;">Cassava is a tropical storage root crop, a source of carbohydrate and alternative energy. It has been classified as “drought tolerant plant” for the whole life cycle, except during the root initiation stage (120-150 DAP). Leaf area index (LAI) is one of the most parameters representing the overall growth and yield prediction in cassava. The aim of this investigation was to validate the physiological and growth performance of cassava in responses to water deficit stress in the field trial as well as to investigate the leaf area index as an important factor to cassava growth and storage root bulking. Leaf relative water content in cassava declined significantly upon a long period of water withholding, and regulated non-photochemical quenching (NPQ), leading to chlorophyll degradation, reduced number of leaves and limited leaf area index (LAI) and loss of storage root yield when compared with well-irrigated plants. Non-destructive leaf area estimation model under water deficit stress condition using spectral reflectance to determine the LAI and VIs was validated. The Ratio Vegetation Index (RVI) was suitable model with high coefficient of determination (R<sup>2</sup> = 0.89). However, the RVI as LAI at 150 DAP (120 d water withholding) could be considered as the critical point to indicate cassava growth and yield performance. Based on the results, cassava growth, biomass and yield in the different environments may further be investigated, taking into consideration the genotypic variation and using remote sensing technology for rapid monitoring and accurate and cost-effective data assessment.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-04-10T00:00:00+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11475 The Productive Capacity and Quality of Several Walnut Cultivars (Juglans regia L.) Grown in North Oltenia, Romania 2019-04-19T17:24:18+00:00 Mihai BOTU btmihai@yahoo.com Yazan F.J. ALABEDALLAT yazan.fja@gmail.com Felicia BUCURA felicia.bucura@icsi.ro Elisabeta Irina GEANA irina.geana@icsi.ro Marius VLADU mariusvladu@yahoo.fr <p style="text-align: justify;">Romania has tradition in walnut culture and favorable climatic conditions. In order to establish the suitable cultivars for growing into the Northern Oltenia area several walnut cultivars of different origin were evaluated for productivity and fruits quality. Twelve Romanian cultivars and two foreign ones with terminal bearing and 10 cultivars with lateral bearing from France and U.S.A. were studied during 18 years of yielding with no irrigation. The group of lateral bearing cultivars proved to be more productive in the environmental conditions of the study area, the highest average yields were recorded for ‘Vina’ (3.64 t ha<sup>-1</sup>), ‘Hartley’ (3.59 t ha<sup>-1</sup>), ‘Ferjean’ (3.52 t ha<sup>-1</sup>), ‘Payne’ (3.45 t ha<sup>-1</sup>), ‘Pedro’ (3.39 t ha<sup>-1</sup>), etc. The most productive terminal bearing cultivars were: ‘Jupânești’ (2.88 t ha<sup>-1</sup>), ‘Valcor’ (2.77 t ha<sup>-1</sup>), ‘Franquette’ (2.71 t ha<sup>-1</sup>), ‘Valcris’ (2.62 t ha<sup>-1</sup>), etc. Although the lateral bearing cultivars emphasized good yields, their extensive culture into the areas with continental type of climatic conditions should be further analysed. The walnut kernel and oil content show that the cultivars are very similar regarding chemical composition when growing under same conditions in Northern Oltenia.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********<br></strong></p> 2019-04-10T11:53:28+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11433 Priming with Vitamin U Enhances Cold Tolerance of Lettuce (Lactuca sativa L.) 2019-04-19T17:25:00+00:00 Laszlo FODORPATAKI lfodorp@gmail.com Katalin MOLNAR molnarkati02@gmail.com Bernat TOMPA tompa_bernat@yahoo.com Sebastian R. C. PLUGARU sebastian.plugaru@yahoo.com <p style="text-align: justify;">Priming may be an efficient pre-treatment of plants in order to enhance their ability to cope with unfavourable growth conditions, and to improve defensive metabolism through elevated levels of protective substances which may also act as health-promoting agents upon human consumption. The aim of this work was to evaluate the beneficial influence of priming with the naturally occurring, but scarcely known vitamin U (S-methylmethionine) on cold stress tolerance of lettuce (the frequently grown ‘May King’ cultivar). Effects on germination, photosynthetic efficiency, as well as on health-promoting carotenoid and vitamin C contents were investigated. Photosynthetic capacity, strongly related to productivity, was evaluated with parameters of induced chlorophyll fluorescence and of leaf gas exchange through stomata, using plants grown in hydroponic cultures. Priming with vitamin U significantly compensated for the delaying effect of low temperature (5 °C) on seed germination, as well as for inhibition of light-converting photochemical reactions and of carbon dioxide assimilation by cold stress. Use of vitamin U to prime lettuce plantlets for low temperature stress resulted in an elevated content of carotenoid pigments and of vitamin C in leaves, which improve the quality of consumed lettuce with respect to the health-promoting capacity. This beneficial influence of vitamin U was not proportional with its concentration (2 mM had no stronger effects than 0.25 mM), so small amounts of this substance were sufficient for a sustained efficiency in promoting hardening against chilling temperatures. This is the first report on priming of lettuce for cold tolerance by using S-methylmethionine (vitamin U), with a possible application in improvement of crop quality and productivity.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T12:17:48+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11382 Biochemical Characterisation and Sensory Evaluation of Differently Coloured and Shaped Tomato Cultivars 2019-04-19T17:25:40+00:00 Aylin Celile OLUK aylinoluk@yahoo.com Atilla ATA atilla.ata@tarimorman.gov.tr Mustafa ÜNLÜ mustafa.unlu@tarimorman.gov.tr Ebru YAZICI ebru.yazici@tarimorman.gov.tr Zafer KARAŞAHİN zafer.karasahin@tarim.orman.gov.tr Evren Çağlar EROĞLU evrencaglar.eroglu@tarimorman.gov.tr İhsan CANAN ihsancanan@ibu.edu.tr <p style="text-align: justify;">Antioxidant capacity, total phenolic content, colour, sugar, volatiles, ascorbic acid and carotenoid (β-carotene and lycopene) contents of differently coloured and shaped tomato cultivars (cvs) grown in the Eastern Mediterranean region, Turkey were determined, along with a sensory evaluation. Tomato cultivars of two different types (cherry and beefsteak) and four different colours (red, yellow, orange and brown) were analysed. All plants were simultaneously grown in the same field and subjected to identical horticultural practices to minimise the effects of environmental conditions and to maximise those related to genotype. The red cherry cultivar had the highest lycopene content, while the orange beefsteak cultivar had the highest β-carotene content. The highest antioxidant capacity, total phenolic content and hardness scores were found in cherry-type tomatoes, except the yellow one. The red cherry cultivar had the highest sugar content. Red and brown cherry cultivars were also significant in terms of their high carotenoid and sugar contents, along with a high antioxidant activity. The brown cherry cultivar had the highest total phenol content. The highest quantities of 2-hexenal, 3-hexen-1-ol, and 6-methyl-5-hepten-2-one were detected in red cultivars. The brown cherry cultivar had the highest sweetness, typical aroma and hardness scores, while the yellow beefsteak cultivars the lowest sweetness typical aroma scores. In terms of sensory parameters, red and brown cultivars scored higher than yellow and orange ones.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T12:34:31+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11434 Arbuscular Mycorrhiza Improves Leaf Food Quality of Tea Plants 2019-04-19T17:26:25+00:00 Ya-Dong SHAO 1092743294@qq.com De-Jian ZHANG zhangdejian0553@126.com Xian-Chun HU 360233834@qq.com Qiang-Sheng WU wuqiangsh@163.com Chang-Jun JIANG jiangcj@ahau.edu.cn Xiu-Bing GAO gxb527@163.com Kamil KUČA kamil.kuca@uhk.cz <p style="text-align: justify;">Tea (<em>Camellia sinensis</em>) plants inhabit arbuscular mycorrhizal fungi (AMF) in rhizosphere, whereas it is not clear whether AMF improves leaf food quality of tea plants. A potted study was conducted to determine effects of <em>Claroideoglomus etunicatum,</em> <em>Diversispora</em> <em>spurca</em>, <em>D</em>. <em>versiformis </em>and a mixture of the three AMF species on leaf sugar, amino acid, soluble protein, tea polyphenol, catechuic acid, and flavonoid contents of <em>Camellia sinensis</em> ‘Fuding Dabaicha’ seedlings. After 12 weeks of AMF inoculation, mycorrhizal plants recorded significantly higher shoot biomass and total leaf area, whilst the effect was ranked as <em>C</em>.<em> etunicatum</em> &gt; <em>D</em>. <em>spurca </em>&gt; mixed-AMF &gt; <em>D</em>. <em>versiformis</em> in the decreasing order. AMF treatments significantly increased leaf total amino acid concentrations, accompanied with up-regulation of amino acid synthetic enzymes genes glutamine synthetase (<em>CsGS</em>), glutamate synthase (<em>CsGOGAT</em>) and glutamate dehydrogenase (<em>CsGDH</em>). Leaf glucose, sucrose, total soluble protein, tea polyphenol, catechuic acid, and flavonoid contents were significantly higher in AMF- than in non-AMF-inoculated plants. In addition, mycorrhizal inoculation notably up-regulated the expression level of leaf 3-hydroxy-3-methylglutaryl coenzyme gene (<em>CsHMGR</em>)<em>, </em>ascorbate peroxidase gene (<em>CsAPX</em>), and tea caffeine synthase 1 gene (<em>CsTCS1</em>). These results implied that AMF inoculation had positive effects on leaf food quality partly by means of up-regulation of relevant gene expression in ‘Fuding Dabaicha’ seedlings.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T12:49:06+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11394 Investigation of Mineral and Vitamin C Contents in Different Parts of Beetroots (Beta vulgaris L.) 2019-04-19T17:27:07+00:00 Dóra SZÉKELY babinszky-szekely.dora@etk.szie.hu Diána FURULYÁS furulyas.diana@etk.szie.hu Mónika STÉGER-MÁTÉ stegerne.mate.monika@etk.szie.hu <p style="text-align: justify;">Due to the increasing popularity of today’s health-conscious culture, beetroot plays an increasingly important role in a healthy, varied diet. While it contains vitamins B1, B2, B6, P, as well as high levels of vitamin C, several studies have proven its role in the prevention of many cancers. The aim of this study was to compare morphological properties, dry matter, and vitamin C and minerals contents in the different parts (peel, end, middle and top part of root) of the three investigated beetroot cultivars grown in treated and untreated soil. Fertilizer was added to the treated soil before sowing of the beet seeds, while untreated soil was not treated. The vitamin C contents were measured using HPLC method, while determination of element contents was determined with IPC-OES. The study showed that the beetroots grow in the treated soil had improved morphological properties, but a significant difference was not found. For Vitamin C and element contents (Ca, Cu, Fe, K, Mg, Mn, Na, P, Zn), it was observed a high degree of diversity in the different beetroot parts and between the cultivation years. The aim of the study was to investigate the distribution of the examined parameters within the beetroot body in different beetroot cultivars observing the differences produced by the addition of fertilizer. Some elements were concentrated in the peel and top part such as Ca, Mn and Mg. The peel part of the beetroots contained relevantly higher Fe content than the top, middle and end part. Based on these results, significant differences were observed among the investigated beetroot cultivars regards of the body weight.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T12:56:03+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11400 Phytosociology, Distribution and Ecology of a Willow Community with False Tamarisk from the Lotru Valley (Romanian Carpathians) 2019-04-19T17:27:56+00:00 Ilie Silvestru NUȚĂ silvestru1969@yahoo.com Mariana NICULESCU mniculescum@yahoo.com <p style="text-align: justify;">The overall objective of this research was to contribute to a better knowledge of the phytosociology, ecology and distribution of the plant community formed by <em>Myricaria germanica</em> (false tamarisk) and the willow species <em>Salix purpurea.</em> This plant community has an important role in reducing the speed and strength of the water flow during floods. The description was based on cover‐abundance data for the faithful, dominant, characteristic and companion species of this plant community. A predominance of <em>Myricaria germanica</em> was observed when analysing the phytocoenoses, as the species finds in this area favourable ecological conditions for an abundant development.<em> Salix purpurea</em> presents a small abundance-dominance or in some phytocoenoses may even lack. The field research also revealed the excessive development of the species <em>Calamagrostis pseudophragmites</em> in some areas, related mostly with the effect of human activities. The anthropogenic disorders have induced modifications in the floristic physiognomy and compositions of the phytocoenoses, favouring also the invasion of opportunistic species such as <em>Ambrosia artemisiifolia</em>,<em> Impatiens glandulifera</em>,<em> Phytolacca amearicana</em>, <em>Amorpha fruticosa</em>,<em> Erigeron annuus</em>, which continuously extend in the studied territory. As <em>Myricaria germanica</em> and <em>Salix purpurea</em> phytocoenoses suffer a great decline throughout Europe, the habitat built by these species was declared of interest and was included in the Habitats Directive.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T13:01:41+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11419 Optimization of the Solid-Liquid Extraction Process of Phenolic Compounds from Mulberry Fruit 2019-04-19T17:28:55+00:00 Emilija KOSTIĆ emilija293@gmail.com Biljana ARSIĆ ba432@ymail.com Milan MITIĆ milanmitic83@yahoo.com Danica DIMITRIJEVIĆ danicadimitrijevic7@gmail.com Emilija PECEV MARINKOVIC epecev@yahoo.com <p style="text-align: justify;">The study was designed to examine the influence of solvent concentration (ethanol/water 20-80% by volume), extraction time (15-240 min) and extraction technique on the extraction yield of phenolic compounds, flavonoids and antioxidant activity from <em>Morus nigra</em> L., <em>Morus rubra</em> L. and <em>Morus alba</em> L. fruits. The optimum conditions for the extraction of total phenols, flavonoids and monomeric anthocyanins by the maceration and ultrasonic extraction processes were: 213.6 min with 80% ethanol, and 182.1 min and 71.2% ethanol, respectively (black mulberry), and 216.5 min with 78.3% ethanol and 198.7 min and 70.6% ethanol, respectively (red mulberry). In case of white mulberry for the extraction of total phenols and flavonoids optimum extraction conditions (maceration and ultrasonic) were 232.7 min and 80% ethanol, and 187.2 min and 68.7% ethanol, respectively (white mulberry). Obviously, ultrasonic extraction was less time consuming, and it requires for all performed extractions solvent with less percentage of ethanol.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T13:10:14+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11420 Antioxidant Properties and Anti-inflammatory Effects of the Hydroethanolic Extracts of Two Varieties of Bayberry fruit (Myrica rubra Sieb et Zucc.) Prepared by Stirring and Ultrasonic Methods 2019-04-19T17:29:41+00:00 Kuan-Hung LIN rlin52714@yahoo.com.tw Chun-Ping LU 089984@mail.fju.edu.tw Jia-Wei CHAO nsr.tigra@gmail.com Yi-Ping YU yyp@faculty.pccu.edu.tw <p style="text-align: justify;">Chinese bayberry (<em>Myrica rubra </em>Sieb. et Zucc.) is an economically important medicinal plant with multiple uses. Two varieties ‘Dongkui Oriental Pearl’ (Dongkui for short) and <em>acuminata</em> ‘Nakai’ (Nakai for short) were used to compare and evaluate the antioxidant activities of hydroethanolic extracts of the fruit using ultrasonic and stirring extraction methods. Dongkui bayberry fruit extract (BFE) prepared using the ultrasonic method exhibited a significantly higher value for the total phenolic content (TPC) and had lower 50% inhibitory concentration (IC<sub>50</sub>) values of scavenging activities against 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), as well as reducing power compared to the other treatment. The TPC of the BFE was significantly correlated with its DPPH, ABTS, and H<sub>2</sub>O<sub>2</sub> radical-scavenging and reducing power activities. Dongkui BFE at a concentration of 0.25 mg/mL exhibited significantly greater protection of RAW 264.7 mouse macrophages against H<sub>2</sub>O<sub>2</sub>-induced damage and lipopolysaccharide (LPS)-stimulated nitric oxide production by macrophages, and it displayed remarkable inhibitory effects compared to the other extracts using the ultrasonic extraction method. Furthermore, compared to the Nakai BFE, macrophages exposed to the Dongkui BFE by the ultrasonic extraction method significantly inhibited LPS-induced production of tumor necrosis factor-α at a concentration of the extract of 0.25 mg/mL. The antioxidant properties and anti-inflammatory and protective effects of BFE prepared by stirring and ultrasonic methods are discussed for the first time in this study.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T13:19:44+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11425 Tyrosinase Inhibitory and Antioxidant Activity of Wild Prunus spinosa L. Fruit Extracts as Natural Source of Bioactive Compounds 2019-04-19T17:30:51+00:00 Milica I. STANKOVIĆ milica.stankovic@medfak.ni.ac.rs Vesna Lj. SAVIĆ vesna.savic@medfak.ni.ac.rs Jelena V. ŽIVKOVIĆ jelenazi@medfak.ni.ac.rs Vanja M. TADIĆ vtadic@mocbilja.rs Ivana A. ARSIĆ ivana.arsic@medfak.ni.ac.rs <p style="text-align: justify;">In recent years, naturally derived tyrosinase inhibitors and antioxidants have become very important, since the synthetic ones are related with several side effects. As wild fruits are rich source of bioactive compounds, this study focused on the examination of <em>Prunus spinosa </em>L. (blackthorn) fruit extracts, widely distributed in our country. However, blackthorn extracts are not enough investigated, and there are only a few published works. Extraction efficiency of <em>P. spinosa</em> ultrasonic fruit extracts obtained by using different solvents (methanol, 70% (v/v) ethanol, 45% (w/w) propylene glycol and water) was evaluated, regarding polyphenolic profile, tyrosinase inhibitory and antioxidant activity. Results suggested that extracts with 45% propylene glycol and water were particularly rich in polyphenolic compounds (especially phenols, flavonoids and anthocyanins). The highest extraction yield was for 45% propylene glycol extract. This extract showed higher tyrosinase inhibitory and antioxidant activity compared to others, while the methanolic extracts had the highest chelating capacity. Therefore, <em>P. spinosa</em> fruit extracts, especially extracts obtained by ultrasonic extraction with 45% propylene glycol, have a great potential to be incorporated in some pharmaceutical formulations for obtaining tyrosinase inhibitory and antioxidant effects.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T13:26:52+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11440 Physiological Responses and Tolerance Evaluation of Five Poplar Varieties to Waterlogging 2019-04-25T12:00:17+00:00 Zhongcheng ZHOU 1806725680@qq.com Gang LI 15090754721@163.com Wei CHAO 1429175992@qq.com Feng XU xufeng198@126.com Xiaomei SUN 1628172947@qq.com Zexiong CHEN chenzexiong1979@163.com <p style="text-align: justify;">Waterlogging resistance of five poplar varieties, ‘Danhongyang’ (DHY), ‘Juba-261’ (JB-261), ‘Zongqiansanhao’ (ZQ-3), ‘Zhonglin-2025’ (ZL-2025), and ‘Nanlin-895’ (NL-895), was evaluated under the simulated waterlogging conditions. Data on changes in leaf color and morphology as well as in biochemical indices, such as chlorophyll, malonaldehyde, soluble protein, soluble sugar content, superoxide dismutase (SOD), peroxidases (POD), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione peroxidase (GSH-PX) activities, relevant to submergence stress, were analyzed. The principal component analysis of the data identified the waterlogging resistance coefficient of the indices, which showed that waterflooding brought about different degrees of damage in the five poplar varieties, with DHY having the lowest waterlogging index. The leaf pigment content of the poplar was remarkably decreased by waterlogging, whereas malondialdehyde (MDA) and proline contents were enhanced, but in different extents among the poplar varieties. Nearly all other poplar varieties showed a tendency of decline in JB-261, ZQ-3, ZL-2025, NL-895, except for SOD activity in DHY, which increased under submergence stress. Poplar varieties had varying degree of changes in POD activity, and APX activity tended to increase upon waterlogging. GR also displayed increasing tendency in JB-261, ZL-2025 and NL-895, except for in ZQ-3, which declined under waterlogging stress. GSH-PX except for ZQ-3 displayed no significant change, which showed a tendency of decline in DHY, JB-261, ZL-2025, and NL895. Principal component analysis allowed us to reduce16 indices to four independent indices. The subordinate function analysis identified that the DHY variety had the highest waterlogging tolerance, whereas the NL-895 variety had the lowest waterlogging tolerance among tested varieties.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong>*********</strong></p> <p style="text-align: justify;"><strong>In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong>Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="text-align: justify;"><strong>*********</strong></p> 2019-04-10T13:33:45+00:00 Copyright (c) https://www.notulaebotanicae.ro/index.php/nbha/article/view/11407 Biocontrol of Pear Postharvest Decay by Kombucha 2019-04-19T17:33:21+00:00 Junping TAN tanjunping0318@163.com Xian ZHOU chenxitianmu@126.com Shuiyuan CHENG s_y_cheng@sina.com Zexiong CHEN chenzexiong1979@163.com Yuanyuan GOU gouyuanyuan0307@163.com Jiabao YE yejiabao13@163.com Feng XU xufeng198@126.com <p style="text-align: justify;">Kombucha is sweetened, slightly alcoholic, and lightly effervescent tea drink. Its acidity inhibits the growth of harmful bacteria. Here, we studied effect of kombucha on post-harvest preservation of pear. The Hosui pears were soaked with the kombucha for 15 minutes, and in distilled water as the control, respectively. Superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), proline content, electric conductivity, weight loss rate and good fruit rate in pear were measured during storage at room temperature. The results showed that the kombucha could effectively prolong the storage time of pear fruit. The fruit quality of the treated group was 1.5 times higher than that of the control pear fruit when stored at room temperature for 18 days. The weight loss rate, MDA content and electric conductivity of the treatment group were lower than those of the control group during the whole storage period, indicating that kombucha can inhibit the transformation of polysaccharides such as starch and pectin, and delay the degradation of nutrients in the fruit, resulting in a decrease in weight loss rate, inhibition of membrane lipid peroxidation, reducing MDA content, electric conductivity, maintaining cell membrane stability, delaying pear fruit senescence. Kombucha can inhibit the content of H<sub>2</sub>O<sub>2</sub> and proline, increase the activity of POD and SOD increase the storage stability of fruits. The purpose of this study was to elucidate the physiological mechanism of post-harvest preservation of pear by kombucha. The use of kombucha can prolong the supply period of pear, increase economic benefits and expand the market of pear.</p> <p style="text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;"><strong>*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: justify; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;"><strong>*********</strong></p> 2019-04-17T10:23:13+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11388 Cross Compatibility Analysis to Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding 2019-04-19T17:37:30+00:00 Huali ZHANG lilytalk2002@163.com Haibo XIN zghhzpx@163.com Richen CONG hardhopeee@163.com Zijing LI lizijing8314@126.com Lina SONG songln_009@126.com Zunzheng WEI weizunzheng@163.com <p style="text-align: justify;">Seven interspecific crosses of <em>Tagetes erecta</em> × <em>T. patula</em> were conducted in this study<strong>. </strong>The cross compatibility index, seed setting rate, germination rate, phenotype, ploidy level, heterosis over male parent and field performance were tested. The results showed that different cross compatibility indices of <em>Tagetes</em> vary greatly, from 10.44~114.41, which is far less than that of the intraspecific hybridization S-121 × I-506 index. The seed setting rate of each cross ranged from 15.67% to 49.0%, and the cross S-121 × TP-512 scored the highest seed setting rate, which was higher than that of the intraspecific cross S-121 × I-506 (35.85%). Compared with the male parent, F<sub>1</sub> has higher plant height, wider crown width, larger flower diameter and more ray florets and earlier bloom with two exceptions. The S-121 of <em>T. erecta</em> was diploid. All of the male parents of <em>T. patula </em>were tetraploids, and the combinations were triploids. The traits of plant height, plant width, flower size and number of ray florets showed positive heterosis over the male parents, and the days leading to flowering showed both positive and negative ones. Finally, S-121 × TP-379 and S-121 × TP-512 were selected as the two best combinations. This study confirmed that the two species of marigold could be crossed for better F<sub>1</sub> varieties with improved performance.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> 2019-04-17T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11448 Physiological Effects of the Fungicide Azoxystrobin on Wheat Seedlings under Extreme Heat 2019-05-15T06:38:50+00:00 Chiu-Yueh LAN donutstream@gmail.com Kuan-Hung LIN rlin52714@yahoo.com.tw Wen-Dar HUANG wendar@ntu.edu.tw Chang-Chang CHEN chencc@nricm.edu.tw <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Azoxystrobin (AZ) is not only a fungicide used for disease control, but also a protective chemical for crops against specific stresses. The physiological mechanism of the fungicide AZ in protecting against heat (HT, 46 °C) stress in wheat (<em>Triticum aestivum</em> L.) seedlings was investigated. ‘Taichung SEL-2’ variety seedlings were pretreated with 0.4, 4, 40, 80, and 120 mg L<sup>-1</sup> of AZ for 4 d. Next AZ-pretreated and untreated seedlings were subjected to HT for 1 h followed by 1000 μmol m<sup>-2 </sup>s<sup>-1</sup> lighting for 20 min. HT induced oxidant stress which resulted in a decrease in the reducing power, an increase in malondialdehyde, and enhanced enzyme activities of ascorbate peroxidase (APX) and catalase (CAT) in leaves of untreated seedlings. However, AZ-pretreated seedlings under HT displayed reductions in chlorophyll fluorescence, APX and CAT activities, and the 1,1-diphenyl-2-picryl-hydrazyl scavenging capacity. Physiological damage caused by HT was aggravated by an increase in the AZ concentration. In addition, increased photosynthetic pigments were also observed in leaves of AZ-pretreated and HT-exposed seedlings. The results suggest that AZ does not provide a protective effect against HT stress.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-14T00:00:00+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11436 The Propagation of Rosa gallica ‘Tuscany Superb’ by Root Cuttings with the Use of IBA and Biostimulants 2019-05-20T07:47:44+00:00 Marta Joanna MONDER m.monder@obpan.pl Konrad WOLIŃSKI k.wolinski@obpan.pl Maciej NIEDZIELSKI m.niedzielski@obpan.pl <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">The research was aimed at studying the possibility of an alternative method of propagation the difficult-to-root <em>Rosa gallica</em> ‘Tuscany Superb’. The root cuttings of three thickness were rooted using IBA preparations (Chryzotop green, Rhizopon AA 020XX) and biostimulants (Tytanit, Root Juice<sup>TM</sup>). The shoots disappeared before the growth of the roots. At the first days of growth, the both shoots and roots elongated upright without polarity. The primordia of the first type of root/shoot buds were situated in cambium zone as the subtending traces of near the rays visible on the cross sections. Moreover, the second types of buds were located in pericycle tissues. The swellings suggested inherence buds were disappearing before rooting. The content of reducing and non-reducing carbohydrates was lower at the time of harvesting the rooted cuttings than before rooting. The thickness of the root cuttings had a significant importance for growth parameters and content of carbohydrates in rooted cuttings. The most useful root cuttings, those achieving the highest mean rooting percentage are thick (76.8%) and medium (67.9%) size. Preparations or biostimulants had a differential influence on rooting process and none of them unequivocally guaranteed an improvement in the rooting percentage of cuttings, parameters of growth or content of carbohydrates. The Rhizopon AA 020XX (90.0%) in thick and Chryzotop green (87.5%) in medium cuttings increased the rooting percentage significantly. Root Juice<sup>TM</sup> (0.1%, 0.2%) and Tytanit (0.04%) were also enough positive activity in medium cuttings and can replace IBA preparations.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-15T07:42:27+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11333 Low Level of Genetic Variation and Signs of Isolation in the Native Hungarian Sea Buckthorn Population Compared to Cultivated Specimens 2019-05-15T12:42:01+00:00 Mária HÖHN hohn.maria@kertk.szie.hu Magdolna SZELÉNYI szelenyi.magdolna@agrar.mta.hu Júlia HALÁSZ halasz.julia@kertk.szie.hu <p style="text-align: justify;">Sea Buckthorn population from Újpest Nature Reserve, the only one assumed to be native in Hungary sustains at the periphery of Budapest city on the sandy substrate deposited by the Danube river. The study aims to characterize the gene stock preserved on this small edaphic habitat by comparing with the genetic material of planted specimens of known origin used in horticultural and gardening practice. Fragment length variations of seven nuclear microsatellite markers were evaluated and a phylogenetic tree was constructed based on sequence variation of an intron from nuclear gene encoding the chalcone synthase enzyme. Gender rate was evaluated by female specific SCAR marker. Microsatellite analysis pointed out the extremely low variation of the natural sea buckthorn population from Újpest Nature Reserve compared to the cultivated specimens, while the phylogenetic tree revealed that the natural population belongs to the genetic lineage distributed mostly in Western and Middle-Europe, and in Romania, region, which has strong links to the Balkan and the Black Sea. The analysis clearly separated the genetic material of the native Újpest population from the planted individuals from the vicinity that proved to be of allochthonous origin. The HrX2 SCAR marker revealed the dominance of the males (1:1.42). The study provides support for the autochthonous origin of the Hungarian remnant population being in reproductive isolation that suggest for its long persistence and relict status. <em>Ex situ</em> conservation and nature protection measures are highly recommended to maintain one of the last native sea buckthorn populations of the Pannonian region.</p> <p style="text-align: justify;">&nbsp;</p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> <p style="text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; display: inline !important; float: none;">). The article is searchable and citable by Digital Object Identifier (DOI). <span style="text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="text-align: justify;"><strong><span style="color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">*********</span></strong></p> 2019-05-15T11:42:38+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11463 Growth, Physiological and Biochemical Responses of two Greek Cotton Cultivars to Salt Stress and their Impact as Selection Indices for Salt Tolerance 2019-05-15T16:55:11+00:00 Maria-Anna MOUSSOURAKI marianna.mous7@gmail.com Eleni TANI l_granny@yahoo.com Anna VELLIOU annavelliou2@gmail.com Maria GOUFA marog@aua.gr Maria PSYCHOGIOU mariapsy1@gmail.com Ioannis E. PAPADAKIS papadakis@aua.gr Eleni M. ABRAHAM eabraham@for.auth.gr <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">Soil salinity is a major constrain of crop productivity. Upland cotton (<em>Gossypium hirsutum</em> L.) is an important fiber crop worldwide and a major agricultural product in Greece. Two commercial cotton cultivars (‘Hersi’ and ‘ST 318’) were studied to compare their response under non-saline and saline conditions in a greenhouse experiment. Salt stress on plants was imposed by two different approaches: a gradual and an initial acclimatization to a non-lethal NaCl concentration (150 mM). To explore salt stress responses, growth (height of plants, roots, shoots and leaves dry weight, reproductive shoots, Salinity Sensitivity Index), gas exchange (Photosynthetic rate, Stomatal conductance, Transpiration rate and Water Use Efficiency) and biochemical parameters (proline, H<sub>2</sub>O<sub>2</sub> and MDA content), were examined as well as ion homeostasis. ‘Hersi’ had significantly higher dry weight of roots, shoots and leaves, lower salinity sensitivity index of roots compared to ‘ST 318’.&nbsp; In this regard, it appears that ‘Hersi’ cultivar performed better than ‘ST 318’ to increased salinity conditions, due to better control of gas exchange parameters and K<sup>+</sup>/Na<sup>+</sup> homeostasis as well as better membrane integrity. Furthermore, the gradual acclimatization to the 150 mM NaCl concentration had a milder effect on both cultivars compared to the initial acclimatization.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-15T13:08:26+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11437 Effects of Different Shading Rates on the Photosynthesis and Corm Weight of Konjac Plant 2019-05-19T08:54:26+00:00 Yaoguo QIN qinyaoguo@sina.com Zesheng YAN 863748012@qq.com Honghui GU 407959707@qq.com Zhengxiang WANG 1095594263@qq.com Xiong JIANG 2791998606@qq.com Zhizhou CHEN 1628579669@qq.com Fuqing YANG 913188595@qq.com Cuiqin YANG ycuiqin@126.com <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">To study the effects of shading level on the photosynthesis and corm weight of konjac plant, the chlorophyll fluorescence parameters, daily variation of relative electron transport rate (rETR), net photosynthetic rate (<em>P</em>n), and corm weight of konjac plants under different treatments were measured and comparatively analyzed through covered cultivation of biennial seed corms with shade nets at different shading rates (0%, 50%, 70%, and 90%). The results showed that with the increase in shading rate, the maximum photochemical efficiency, potential&nbsp;activity, and non-photochemical quenching of photosystem Ⅱ (PSⅡ) of konjac leaves constantly increased, whereas the actual photosynthetic efficiency, rETR, and photochemical quenching of PSⅡ initially increased and then decreased. This result indicated that moderate shading could enhance the photosynthetic efficiency of konjac leaves. The daily variation of rETR in konjac plants under unshaded treatment showed a bimodal curve, whereas that under shaded treatment displayed a unimodal curve. The rETR of plants with 50% treatment and 70% treatment was gradually higher than that under unshaded treatment around noon. The moderate shading could increase the <em>P</em>n of konjac leaves. The stomatal conductance and transpiration rate of the leaves under shaded treatment were significantly higher than those of the leaves under unshaded treatment. Shading could promote the growth of plants and increase corm weight. The comprehensive comparison shows that the konjac plants had strong photosynthetic capacity and high yield when the shading rate was 50%-70% for the area.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-19T08:52:33+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11450 Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis 2019-05-19T11:22:57+00:00 Xiao Dong CAI caixiao.dong@163.com Jin Jin CHENG Chen_JinJin0@163.com Yuan Shan ZHANG realzys1994@163.com <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"><em>Trichosanthes kirilowii</em> Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for <em>in vitro</em> production of high-quality plantlets of <em>T. kirilowii</em> cv. ‘Yuelou-2’ via organogenesis<em>. </em>Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L<sub>9 </sub>(3<sup>4</sup>) orthogonal array design. MS medium supplemented with 0.5 mg l<sup>-1</sup> 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance <em>in vitro</em> and <em>ex vitro</em> were comprehensively assessed, and results showed that the addition of 1.0 mg l<sup>-1 </sup>NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The <em>in vitro</em> propagation system described here has the potential for large-scale and rapid production of <em>in vitro</em> plantlets of seed variety of <em>T</em>. <em>kirilowii</em> in a sustainable manner.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-19T11:21:27+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11575 Assessment of New Sweet Cherry Cultivars Released at RSFG Iaşi, Romania 2019-05-21T09:10:05+00:00 Elena IUREA iurea_elena@yahoo.com Margareta CORNEANU margaretacorneanu@yahoo.com Mădălina MILITARU madamilitaru77@yahoo.com Sorina SÎRBU sorinas66@gmail.com <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">The research concerning the improvement of the cherry assortment, the cultivars’ quality and the avoiding of market crowding with fresh cherries only on a short term are extremely important objectives for cherries’ breeding. To accomplish some of these objectives, during 1981-2017, hybridization and selection of hybrids were performed at the Research Station for Fruit Growing Iași (RSFG Iași), in the N-E part of Romania, concluding with a series of promising hybrid selections. They have been grafted, planted and studied in trial fields. Therefore, during 1994-2016, RSFG Iași released 28 sweet cherry cultivars with high quality fruits and different maturation stages. In the studies performed during 2014-2017, there were analysed three new sweet cherry cultivars (‘Elaiaşi’, ‘Muşatini’ and ‘Croma’), obtained through controlled hybridization. The comparison of these new cultivars was performed against ‘Van’, used as control, this being a well-known cultivar in the orchards from the study area. Observations and measurements concerning the main phenological stages, natural fertility, physical features, organoleptic, quality and chemical traits of fruit were registered. The cultivars with the biggest dimensions of the fruits were ‘Elaiaşi’ and ‘Croma’ (with a weight between 8.9 and 9.4 g and a fruits’ diameter between 25.2 and 26.6 mm), having significantly larger dimensions in comparison with ‘Van’. In the new investigated cherry cultivars, the values of soluble solids content were comprised between 17.0 and 20.2 °Brix, titratable acidity was between 0.46 - 0.57 mg of malic acid 100 mL<sup>-1</sup> juice and the total polyphenols content was recorded between 382.87 - 780.25 mg GAE 100 mL<sup>-1</sup> of fresh juice.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-21T09:10:02+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11486 Efficient Approaches to in vitro Multiplication of Lilium candidum L. with Consistent and Safe Access throughout Year and Acclimatization of Plant under Hot-Summer Mediterranean (Csa Type) Climate 2019-05-21T08:47:39+00:00 Sheida DANESHVAR ROYANDAZAGH sdaneshvar@nku.edu.tr <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Lilium<em> candidum</em> L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of <em>L. candidum</em> are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective <em>in vitro </em>plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 µM Thidiazuron + 1.08 µM Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 µM TDZ + 1.08 µM NAA were successfully rooted using 1/2 × MS medium containing 2.45 µM indole butyric acid. Regardless of rooting development, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey.</span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">In press - Online First</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (</span><strong style="box-sizing: border-box; font-weight: bolder; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial;">Volume 47, Issue 3, 2019</strong><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; float: none; display: inline !important;">). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue.</span></span></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial; background-color: #ffffff; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;"><span style="box-sizing: border-box; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">*********</span></strong></p> 2019-05-20T17:46:13+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca https://www.notulaebotanicae.ro/index.php/nbha/article/view/11500 Optimization and Characterization of Extracellular Cellulase Produced by Native Egyptian Fungal Strain 2019-05-21T14:41:33+00:00 Gamal El-BAROTY elbarotys@hotmail.com Faten ABOU-ELELLA faten2010bio@yahoo.com Hassan MOAWAD hassanmoawad@hotmail.com Talaat N. El-SEBAI talaatelsebai@gmail.com Fatma ABDULAZIZ fatma.ali@agr.cu.edu.eg A. A. KHATTAB khattababdelnasser69@yahoo.com <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">Since accumulation of agricultural wastes represents a huge problem, it was important to explore the available methods to help eliminate agricultural wastes safely, and simultaneously produce functional enzyme like cellulase. Six native Egyptian fungal strains were isolated, morphologically identified and screened for cellulose biodegradation potential, which was determined as endoglucanase or as carboximethylcellulase (CMCase). The most promising isolate (<em>Aspergillus terreus</em>) was selected for molecular characterizations based on sequencing of internal transcribed spacer (ITS). Further optimization experiments were accomplished on the selected strain. The strain with cellulolytic activity, 2.26 IU mL<sup>-1</sup> was identified using ITS nucleotides (genes) sequences and the result confirmed that the strain is 99.8% homology with<em> A. terreus.</em> Then, it was submitted to GeneBank and given an accession number. Further optimization experiments revealed that 35ºC is the optimum temperature for cellulase production and raised the enzyme activity (EA) up to 3.19 IU mL<sup>-1</sup>. Out of two organic nitrogen sources; peptone at concentration 6 gL<sup>-1</sup> was found to be the optimum nitrogen source for cellulase production with the highest activity 4 IU mL<sup>-1</sup>. Whereas, the different four carbon sources: microcellulose, corn stalks, wheat straw and rice straw showed significant differences in EA with values 11.07, 9.68, 7.87 and 3.71 IU mL<sup>-1</sup>, respectively at&nbsp; pH 3. The maximum EA was recorded to be within 5-7 days of incubation, dependent on type of carbon sources. The optimization of different incubation conditions raised cellulolytic activity from 2.26 IU mL<sup>-1</sup> up to 11.18 IU mL<sup>-1</sup>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;">&nbsp;</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">In press - Online First</strong>. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (<strong style="box-sizing: border-box; font-weight: bolder;">Volume 47, Issue 3, 2019</strong>). The article is searchable and citable by Digital Object Identifier (DOI).&nbsp;<span style="box-sizing: border-box; text-decoration: underline;">DOI link will become active after the article will be included in the complete issue</span>.</p> <p style="box-sizing: border-box; line-height: 25px; margin: 20px 0px 0px; color: rgba(0, 0, 0, 0.87); font-family: 'Noto Serif', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen-Sans, Ubuntu, Cantarell, 'Helvetica Neue', sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; text-align: justify;"><strong style="box-sizing: border-box; font-weight: bolder;">*********</strong></p> 2019-05-21T14:41:31+00:00 Copyright (c) 2019 Notulae Botanicae Horti Agrobotanici Cluj-Napoca