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    In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. The article is to be paginated when the complete issue will be ready for publishing (Volume 49, Issue 1, 2021). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.




  • Changes in plant growth, leaf relative water content and physiological traits in response to salt stress in peanut (Arachis hypogaea L.) varieties (2021-01-05)


    Tekam L. MEGUEKAM
    University of Yaounde I, Higher Teacher Training College, Department of Biological Sciences, P.O. Box 47 Yaounde
    Dany P. MOUALEU
    Leibniz University, Institute of horticultural Production Systems, Department of Modeling of Vegetable Systems, 30419 Hannover
    Victor D. TAFFOUO
    University of Douala, Department of Botany, Faculty of Sciences, P.O. Box 24157 Douala
    Hartmut STÜTZEL
    Leibniz University, Institute of horticultural Production Systems, Department of Modeling of Vegetable Systems, 30419 Hannover

    DOI: 10.15835/nbha49112049 


    Salinity is the main environmental factor accountable for decreasing crop productivity worldwide. The effects of NaCl salinity on plant growth (leaf relative water content (RWC), leaf dry weight (LDW), shoot length (SL), number of leaves (NL), number of branches (NB) and total leaf area (TLA) and physiological characteristics (stomatal conductance (gs), transpiration rate (TR), net photosynthetic (Pn), yield of photosystem II (ΦPsII) and the inter-cellular CO2 concentration (CO2int) in peanut (Arachis hypogaea L.) varieties (‘Vanda’, ‘P244601’ and ‘Pl184948’, widely used in Cameroon, Tanzania and Ghana, respectively, were investigated under hydroponic condition. Plants were subjected to four levels of NaCl (0, 40, 80 and 120 mM) at early seedling growth stage of plant development. Application of NaCl treatment led to a significant decrease in LDW, SL, NL, TLA, Pn, gs, TR and CO2int concentration of ‘Vanda’ and ‘P244601’ compared to untreated plants while the plant growth inhibition was notably noted at 120 mM NaCl in ‘P1184948’ for LDW, SL and NB. The highest depressive effect was detected in gs of salt-sensitive ‘Vanda’ while the lowest were recorded in gs of salt-tolerant ‘P1184948’ at high salinity level. Enhanced NaCl concentrations led to a significant increase in ΦPSII of ‘P1184948’ compared to ‘Vanda’, ‘P244601’ and untreated plants. Leaf CHL content was significantly increased in moderately-tolerant ‘‘P244601’ and salt-tolerant ‘P1184948’ at 80 mM NaCl compared to salt sensitive ‘Vanda’ and untreated plants. The depressive effect of salt on RWC was recorded at 120 mM NaCl in peanut leaves of all varieties. Under salt stress ‘P1184948’ was observed to have relatively higher tolerance on average of all growth and physiological traits than ‘Vanda’ and P244601’ suggesting that it could be grown in salt-affected soils.





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    Influence of fertilizer and salicylic acid treatments on growth, physiological, and antioxidant characteristics in green and red Perilla frutescens varieties (2021-01-04)


    Yin-Jheng JHOU
    National Taiwan University, Department of Horticulture and Landscape Architecture, Taipei 106
    Kuan-Hung LIN
    Chinese Culture University, Department of Horticulture and Biotechnology, Taipei 111
    Chun-Wei WU
    National Taiwan University, Department of Horticulture and Landscape Architecture, Taipei 106
    Yu-Sen CHANG
    National Taiwan University, Department of Horticulture and Landscape Architecture, Taipei 106

    DOI: 10.15835/nbha49112064


    Perilla is herbaceous plant, functional food, and nutraceutical product with antioxidant properties. The objective of this study was to investigate the growth, reflectance indices, and antioxidant properties of P. frutescens species in response to fertilizer and salicylic acid (SA) applications. Two independent experiments were carried out in an environmentally controlled greenhouse: (1) pots of red-leaf and green-leaf cultivars divided into four groups treated with 10-30-20 (10N-13.1P-16.6K), 15-10-30 (15N-4.4P-24.9K), 20-20-20 (20N-8.7P-16.6K), and 30-10-10 (30N-4.4P-8.3K) fertilizers for periods of 10 weeks, and (2) pots of red and green Perilla cultivars divided into five groups treated with 0 (control), 125, 250, 500, and 1,000 µM of SA for periods of 7 weeks. Wide variations occurred in the agronomic performance, soil-plant analysis development (SPAD) value, adjusted normalized difference vegetation index (NDVI), maximal quantum yield of PSII photochemistry (Fv/Fm), and antioxidant activity of the two Perilla varieties. All the measured traits were higher in green than in red Perilla under identical fertilizing, and all agronomic traits in green and red Perilla plants subjected to 125 and 500 μM SA were better than in controls. The SPAD and NDVI values of all plants increased as N% increased, the lowest Fv/Fm values of all plants were observed under 15-10-30 fertilizer treatment, the lowest NDVI values were detected in controls, and the Fv/Fm values of all plants decreased under 1,000 μM SA treatment. These indices can be used as indicators to characterize the physiology of these plants and are suitable for evaluating their growth and development under specific fertilizer and SA treatments. Green Perilla leaf extract (PLE) contained higher rosmarinic acid (RA) concentration in each fertilizer treatment, and higher total phenolic (TP) and RA concentration in each SA treatment. However, red PLE contained higher caffeic acid (CA) concentration than green PLE in each fertilizer and SA treatment, implying that their two genotypes exhibited different abilities and specificities of photosynthetic metabolites, and that different varieties may prepare for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity by up-regulating TP, RA, and CA concentration differently. Thus, Perilla plants can be used as health foods due to high TP, RA, and CA concentration. To produce Perilla efficiently in industrial applications, we undertook to determine the optimum N-P-K fertilizer ratio and SA application for maximizing the growth and accumulation of TP, RA, and CA in Perilla plants.





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    Filamentous fungi isolated from Tunisian olive mill wastes: use of solid-state fermentation for enzyme production (2021-01-04)


    Hanen ZAIER
    Laboratory of Integrated Olive Production, Olive Tree Institute, Hedi Karray Street, P.O. Box 208, 2080, Ariana
    Sameh MAKTOUF
    Laboratory of Sustainability of Olive Growing in Semi-Arid and Arid Regions: Improvement of Olive Productivity and Product Quality, Olive Tree Institute, Sfax
    Sevastianos ROUSSOS
    Aix Marseille University, Avignon University, CNRS, IRD, IMBE, Marseille
    Ali RHOUMA
    Laboratory of Integrated Olive Production, Olive Tree Institute, Hedi Karray Street, P.O. Box 208, 2080, Ariana

    DOI: 10.15835/nbha49112125


    Olive mill wastewaters and olive cake are effluents generated during olive oil production process. They represent a major disposal and potentially severe pollution problem for the industry, also promising source of substances of high value. The aim of this study is the valorization of olive mill wastes (OMWW, olive cake, olive twigs and leaves) to produce enzymes with high industrial and biotechnological potential, by the solid-state fermentation technique (SSF), from isolated fungi present in olive mill wastewater and olive cake. A total of 47 strains were isolated and purified from these two residues. The metabolic potential of isolated strains was study by testing the hydrolytic enzymes activities of lipase, protease, amylase, cellulase, invertase, phytase and tannase on agar plate media containing different substrate. The monitoring of SSF has shown that the metabolic activity of these strains is extremely rapid using this technique. Our fungi collection contains a diversity of strains capable to producing a variety of enzymes of biotechnological interest.





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    Morphometric analysis and sequence related amplified polymorphism determine genetic diversity in Salvia species (2021-01-14)


    Abdul SHAKOOR
    Henan University, College of Environment and Planning, Kaifeng, 475004, Henan
    Fang ZHAO
    Henan University, College of Environment and Planning, Kaifeng, 475004, Henan
    Gul ZAIB
    Yangzhou University, Institute of Epigenetics and Epigenomics, Yangzhou
    Wuyang LI
    Henan University, College of Environment and Planning, Kaifeng, 475004, Henan
    Xincan LAN
    Henan University, College of Environment and Planning, Kaifeng, 475004, Henan
    Somayeh ESFANDANI-BOZCHALOYI
    Shahid Beheshti University, Faculty of Life Sciences and Biotechnology, Department of Plant Sciences, Tehran

    DOI: 10.15835/nbha49112153


    Salvia species is a member of the Lamiaceae family, and it also possesses medicinal and horticulture values. The genetic diversity was assessed through sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Salvia species were studied through a combination of morphological and molecular data. One hundred forty-five individuals related to 30 Salvia were collected in 18 provinces. A total of 157 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction (PCR) from 30 Salvia species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 10 to 20. The predicted unbiased heterozygosity (H) varied between 0.11 (Salvia urmiensis) and 0.31 (Salvia limbata). High Shannon’s information index was detected in Salvia limbata. The genetic similarities between 30 species are estimated from 0.46 to 0.91. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Salvia hydrangea and Salvia sharifii had the lowest similarity. Salvia bracteata and Salvia suffruticosa were genetically dissimilar to each other. This study also detected a significant signature of isolation by distance. Present results showed that sequence-related amplified polymorphism has the potential to decipher genetic affinity between Salvia species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.