TY - JOUR AU - SAMARFARD, Samira AU - KADIR, Mihdzar A. AU - KADZIMIN, Saleh B. AU - RAVANFAR, Seyedali AU - SAUD, Halimi M. PY - 2013/05/28 Y2 - 2024/03/29 TI - Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 41 IS - 1 SE - Research Articles DO - 10.15835/nbha4118315 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/8315 SP - 177-183 AB - <p><span style="font-size: x-small;">Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of </span><em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;">Phalaenopsis gigantea </span></span></em><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"><span style="font-family: Garamond Premr Pro,Garamond Premr Pro; font-size: x-small;"> </span></span><span style="font-size: x-small;">protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.</span></p> ER -