TY - JOUR AU - XU, Wenting AU - ZHANG, Miao AU - WANG, Chen AU - LOU, Xiongzhen AU - HAN, Xiao AU - ZHANG, Junhong AU - ZHANG, Yuting AU - TONG, Zaikang PY - 2020/06/30 Y2 - 2024/03/29 TI - Somatic embryo induction and Agrobacterium-mediated transformation of embryonic callus tissue in Phoebe bournei, an endangered woody species in Lauraceae JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 48 IS - 2 SE - Research Articles DO - 10.15835/nbha48211946 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/11946 SP - 572-587 AB - <p style="text-align: justify;"><em>Phoebe bournei</em>, a plant species endemic to China, is a precious timber tree and widely used in landscaping. This tree contains numerous secondary metabolites, underscoring its potential economic value. However, studies on this species, including molecular genetic research, remain limited. In this study, both a somatic embryogenesis (SE) technical system and <em>Agrobacterium</em>-mediated genetic transformation were successfully employed in<em> P. bournei</em> for the first time. The SE technical system was constructed using immature embryos as original material. The primary embryo and embryonic callus induction rates were 30.66% and 41.67%, respectively. The highest rate of embryonic callus proliferation was 3.84. The maximum maturity coefficient and germination rate were 53.44/g and 39%, respectively. <em>Agrobacterium</em>-mediated genetic transformation was performed using the SE technical system, and the highest transformation rate was 11.24%. The results presented here are the first to demonstrate an efficient approach to achieve numerous <em>P. bournei</em> plantlets, which serves as the basis for artificial cultivation and resource conservation. Furthermore, the genetic transformation platform constructed in this study will facilitate assessment of gene function and molecular regulation.</p> ER -