TY - JOUR AU - XU, Wenting AU - ZHANG, Yan AU - GUO, Liqin AU - ZHAO, Jian AU - WEI, Zunzheng AU - ZHANG, Jinfeng PY - 2019/07/10 Y2 - 2024/03/29 TI - High frequency 2n pollen formation in black poplar (Populus nigra L.) induced by colchicine JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 47 IS - 3 SE - Research Articles DO - 10.15835/nbha47311521 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/11521 SP - 939-946 AB - <p style="text-align: justify;"><em>Populus nigra </em>L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent <em>Populus nigra </em>clones are identified as triploids potentially obtaining by hybridization of <em>2n</em> pollen and normal oogamete. This study revealed the cytological mechanism of <em>2n</em> pollen formation in <em>Populus nigra </em>L. for the first time and concluded the best treated combination of colchicine treatment, which obtained <em>2n</em> pollen with the highest rate reaching at 87.11% (even 100% <em>2n</em> pollen in some floral buds) which made the polyploid hybridization utilizing <em>2n</em> pollen in section<em> Aigeiros</em> possible.</p> ER -