TY - JOUR AU - BATOOL, Maria AU - AKKAYA, Özlem AU - GÜL ŞEKER, Mine AU - ÖZDEN ÇİFTÇİ, Yelda PY - 2019/07/19 Y2 - 2024/03/29 TI - Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 47 IS - 3 SE - Research Articles DO - 10.15835/nbha47311488 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/11488 SP - 954-961 AB - <p style="text-align: justify;">The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term <em>in vitro</em> cultured fraser photinia microshoots, on seed and 7-day old seedling stages of <em>Arabidopsis thaliana</em>. Seeds and <em>in vitro</em>-germinated seedling<em>s</em> were inoculated with 10<sup>7</sup> CFU/mL and 10<sup>8</sup> CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll <em>a, b</em> and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of <em>virD1</em> gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for <em>A. thaliana</em> when optimum conditions and concentrations are used. Moreover, presence of <em>virD1</em> gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.</p> ER -