TY - JOUR AU - ZHU, Li AU - MA, Liang-Qiong AU - XU, Feng AU - YAN, Xiao-Juan AU - ZHANG, Wei-Wei PY - 2018/01/01 Y2 - 2024/03/28 TI - Cloning and Expression Analysis of a Squalene Epoxidase Gene from Ginkgo biloba JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 46 IS - 1 SE - Research Articles DO - 10.15835/nbha46110868 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/10868 SP - 39-44 AB - Squalene epoxidase is a key enzyme involved in triterpene saponins biosynthetic pathways in plants. In this study, the <em>SE</em> gene was isolated from <em>Ginkgo biloba</em> by RT-PCR, which designated as <em>GbSE</em>, GenBank accession number: KY751713. The length of <em>GbSE</em> gene is 1646 bp, contains an open reading frame of 1617 bp encoding 538 amino acids. The theoretical molecular weight and pI of GbSE protein was 58.3 kDa and 8.35, respectively. Sequence multiple alignment found that GbSE protein had high homology with SE proteins from other plants, including several highly conservative motifs and amino acids. Phylogenetic tree analysis showed that <em>Ginkgo biloba</em> SE belonged to the gymnospermous group, and was closely related to the SE protein of <em>Picea sitchensis</em>. A study of gene expression analysis indicated that the <em>GbSE</em> gene was highly expressed in stems, low expressed in fruits. The cDNA sequence of <em>GbSE </em>gene was cloned from <em>G. biloba</em> and the expression level of this gene was analyzed. This work can provide some theoretical basis for the research on the molecular mechanism of triterpenoid saponin biosynthesis. ER -