TY - JOUR AU - PAVLOVIC, Snežana AU - RISTIC, Danijela AU - VUCUROVIC, Ivan AU - STEVANOVIC, Miloš AU - STOJANOVIC, Saša AU - KUZMANOVIC, Slobodan AU - STAROVIC, Mira Sava PY - 2016/12/14 Y2 - 2024/03/29 TI - Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia JF - Notulae Botanicae Horti Agrobotanici Cluj-Napoca JA - Not Bot Horti Agrobo VL - 44 IS - 2 SE - Research Articles DO - 10.15835/nbha44210488 UR - https://www.notulaebotanicae.ro/index.php/nbha/article/view/10488 SP - 411-417 AB - <p>Anise (<em>Pimpinella anisum</em> L.) is an important medicinal spice plant that belongs to the family <em>Apiaceae</em>. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (MoÅ¡orin, Veliki Radinci and Ostojićevo) during 2012 and 2013, it was found out that <em>Fusarium</em> spp. were a commonly observed fungi. The presence of <em>Fusarium</em> fungion the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of <em>Fusarium</em> species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of <em>Fusarium. </em>Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: <em>F. tricinctum</em>, <em>F. proliferatum</em>, <em>F. equiseti</em>, <em>F. oxysporum</em>, <em>F. sporotrichoides</em>, <em>F</em>. <em>incarnatum</em> and <em>F. verticillioides. </em>According to our knowledge and research, this is the first report of <em>F. tricinctum</em> and <em>F. sporotrichoides </em>as pathogens on anise seeds in the world. All seven isolates of <em>Fusarium</em> species are pathogenic to the anise seedlings, while the most virulent species were<em> F. oxysporum</em>, <em>F. tricinctum</em> and F. <em>incarnatum</em>.</p> ER -