@article{CURTU_MOLDOVAN_ENESCU_CRACIUNESC_SOFLETEA_2011, title={Genetic Differentiation between Quercus frainetto Ten. and Q. pubescens Willd. in Romania}, volume={39}, url={https://www.notulaebotanicae.ro/index.php/nbha/article/view/5633}, DOI={10.15835/nbha3915633}, abstractNote={Little is known about genetic differences among Quercus frainetto and Q. pubescens, two species of section Dascia Kotschy (subgenus Lepidobalanus, white oaks) that reach in Romania the margins of their natural distribution range. A set of genomic SSRs (simple sequence repeats) and EST (expressed sequence tags)-SSRs was used to estimate the genetic differentiation among four natural populations of the two species. Q. pubescens had higher values of genetic diversity than Q. frainetto, although the differences were not significant. Two out of seven marker-loci, QrZAG112 and QpZAG110, displayed very high FST values. Averaged across loci, the genetic differentiation was high and significant (FST = 0.067). Genetic distances were much higher among species than among populations within species. A Bayesian analysis indicated that two is the most appropriate number of genetic clusters. Using a blind procedure (i.e. based on multilocus genotypes only) the vast majority of sampled individuals (90%) could be assigned to the cluster corresponding to their phenotypes. When information about sampling localities was introduced in the assignment test, all individual trees were correctly classified. The higher degree of admixture in Q. frainetto as compared to Q. pubescens may be explained by different rates of introgressive hybridization.}, number={1}, journal={Notulae Botanicae Horti Agrobotanici Cluj-Napoca}, author={CURTU, Alexandru Lucian and MOLDOVAN, Ioan Calin and ENESCU, Mihai Cristian and CRACIUNESC, Iacob and SOFLETEA, Nicolae}, year={2011}, month={May}, pages={275–282} }