@article{GOLUBOVIĆ_STOJANOVIĆ_KITIĆ_ZLATKOVIĆ_PAVLOVIĆ_JOVANOVIĆ_LAZAREVIĆ_2020, title={Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities}, volume={48}, url={https://www.notulaebotanicae.ro/index.php/nbha/article/view/11782}, DOI={10.15835/nbha48111782}, abstractNote={<p style="text-align: justify;">The ethanol extracts of selected <em>Acinos</em> Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of <em>A. majoranifolius, A. suaveolens </em>and<em> A. alpinus </em>were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the <em>A. arvensis</em> and <em>A. graveolens</em> extracts reached 28.97% and 30.75%. Also, <em>n</em>-alkanes were the major compounds found in<em> A. hungaricus </em>ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of <em>A. alpinus</em> which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: <em>Bacillus subtilis</em>,<em> Sarcina lutea</em>, <em>Staphylococcus aureus</em>, <em>Clostridium pyogenes</em>, <em>Enterococcus</em> sp<em>.</em>, <em>Micrococcus flavus</em>; Gram-negative: <em>Klebsiella pneumoniae</em>, <em>Pseudomonas aerugionsa</em>, <em>Salmonella enteritidis</em>, <em>Proteus vulgaris</em>, <em>Escherichia coli</em> and fungal organisms: <em>Aspergillus niger</em>, <em>Candida albicans</em> and <em>Saccharomyces cerevisiae</em> were used as test microorganisms. The results of preliminary bioassay demonstrated that the <em>A. alpinus</em> ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.</p>}, number={1}, journal={Notulae Botanicae Horti Agrobotanici Cluj-Napoca}, author={GOLUBOVIĆ, Tatjana D. and STOJANOVIĆ, Gordana S. and KITIĆ, Dušanka V. and ZLATKOVIĆ, Bojan K. and PAVLOVIĆ, Dragana R. and JOVANOVIĆ, Snežana Č. and LAZAREVIĆ, Jelena S.}, year={2020}, month={Mar.}, pages={53–65} }