Regeneration of Haploid Plantlet through Anther Culture of Chrysanthemum (Dendranthema grandiflorum)

Abstract

To observe the possibility of producing haploid plants of Chrysanthemum, anthers of three Korean cultivars 'Yes Morning', 'Hi-Maya', and pot cultivar 'Peace Pink' were cultured. Callus induction among cultivars differed little, but equally good results were obtained with the basal MS medium supplemented with 1 mg/L of 2,4-D, 2 mg/L of BA, 250 mg/L of casein hydrolysate, 45 g/L of sucrose; solidified by 2.75 g/L gelrite. A pretreatment of anthers in media at 4 °C for 48h enhanced the callus induction. Calli were allowed to differentiate on basal MS medium supplemented with 2 mg/L of BA, 0.1 mg/L of NAA, 30 g/L of sucrose; solidified by 2.75 g/L gelrite.  Shoot formation from calli in that media slightly differed among cultivars. Multiple shoots elongated from calli were shifted to basal MS medium supplemented with 0.1 mg/L of NAA, 30 g/L of sucrose; solidified by 3 g/L gelrite for rooting. The plantlets with sufficient roots thus obtained were acclimatized and transferred to the soil. Fifty regenerated plantlets from each cultivar were randomly selected for ploidy observation by chromosome counting and haploid plantlet was detected for the garden cultivar 'Yes morning'.