Promoter Analysis and Transcriptional Profiling of Ginkgo biloba 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (GbHMGR) gene in Abiotic Stress Responses

Authors

  • Yongling LIAO College of Forestry, Nanjing Forest University, Nanjing, 210037 (CN)
  • Feng XU College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 (CN)
  • Xiaohua HUANG Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000 (CN)
  • Weiwei ZHANG College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 (CN)
  • Hua CHENG Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000 (CN)
  • Linling LI Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000 (CN)
  • Shuiyuan CHENG School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023 (CN)
  • Yongbao SHEN College of Forestry, Nanjing Forest University, Nanjing, 210037 (CN)

DOI:

https://doi.org/10.15835/nbha4319416

Abstract

The terpene trilactones (TTLs) are believed to be important for the pharmacological properties of Ginkgo biloba leaves extract. 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is a critical enzyme involved in the biosynthetic pathway of TTLs. In this study, an 1.2-kb fragment of 5’ flanking region of the HMGR gene (GbHMGR), was isolated from G. biloba by genome walking. Extensive sequence analysis revealed the presence of evolutionarily conserved and over-represented putative cis-acting elements in light-regulated transcription,  hormone signaling (gibberellic acid, jasmonate and salicylic acid), elicitor and stress responses (cold/dehydration responses), and plant defense signaling (W-box/WRKY) that are common to the promoter region of GbHMGR. EMSA analysis suggested possible functionality of W-box in GbHMGR promoter region. The behavior of gene transcripts in ginkgo callus upon light, low temperature, MeJA and SA treatments further verified the regulatory function of GbHMGR promoter. A significant positive relationship between gene expression level and total TTL contents suggested that GbHMGR might be one of key genes involved in TTL biosynthesis in G. biloba.

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Published

2015-05-16

How to Cite

LIAO, Y., XU, F., HUANG, X., ZHANG, W., CHENG, H., LI, L., CHENG, S., & SHEN, Y. (2015). Promoter Analysis and Transcriptional Profiling of Ginkgo biloba 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (GbHMGR) gene in Abiotic Stress Responses. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1), 25–34. https://doi.org/10.15835/nbha4319416

Issue

Vol. 43 No. 1 (2015)

Section

Research Articles
CITATION
DOI: 10.15835/nbha4319416

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Copyright (c) 2015 Yongling LIAO, Feng XU, Xiaohua HUANG, Weiwei ZHANG, Hua CHENG, Linling LI, Shuiyuan CHENG, Yongbao SHEN

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).

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