Production of Flavonoids and Terpene Lactones from Optimized <i>Ginkgo biloba</i> Tissue Culture

  • Shuiyuan CHENG College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, 210037
  • Weiwei ZHANG College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, 210037
  • Nannan SUN College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei
  • Feng XU College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei
  • Linling LI Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, Hubei
  • Yongling LIAO College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China
  • Hua Cheng Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, Hubei

Abstract

This study investigated the effects of various culture conditions on the growth and the production of flavonoids and terpene lactones in the callus of Ginkgo biloba. Callus induced from embryos displayed distinct morphological and physiological responses. MS medium with different plant growth regulators showed a significant effect on the quality and growth of callus. The optimal medium for inducing embryo-derived callus was MS with 2.0 mg/L naphthalene acetic acid (NAA) and 2.0 mg/L 6-benzylaminopurine (6-BA), and the culture medium MS+NAA (2.0 mg/L)+6-BA (1.0 mg/L) was better for the subculture of callus than other culture media tested in this study. In addition, both plant growth regulators and subculturing cycle strongly influenced the production of flavonoids and terpene lactones in the callus. The best subculturing cycle and the optimum culture medium for production of flavonoids and terpene lactones was 30 d and MS+NAA (2.0 mg/L)+6-BA (1.0 mg/L), respectively. These findings provided an important technical support for obtaining the callus cell line from G. biloba embryo that is the richest in flavonoids and terpene lactones.

Author Biography

Feng XU, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei
College of Horticulture and Gardening
Published
2014-06-03
How to Cite
CHENG, S., ZHANG, W., SUN, N., XU, F., LI, L., LIAO, Y., & Cheng, H. (2014). Production of Flavonoids and Terpene Lactones from Optimized <i>Ginkgo biloba</i&gt; Tissue Culture. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(1), 88-93. https://doi.org/10.15835/nbha4219393
Section
Research Articles

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