Development of SCAR Marker Related to Summer Stress Tolerance in Tall Fescue (Festuca arundinacea)

Abstract

Summer stress tolerance (SST) is one of the most important breeding objectives in tall fescue (Festuca arundinacea), an important perennial cool-season grass. However, breeding for better SST is generally complicated by the many environmental factors involved during the growing season. Utilizing the bulked segregant analysis (BSA), we were able to identify one marker related to SST from 100 inter-simple sequence repeat (ISSR) markers and 800 random amplified polymorphic DNA (RAPD) markers, and successfully developed a dominant sequence characterized amplified region (SCAR) marker T_SC856 from the UBC856 sequence. Furthermore, the SCAR marker was tested in different clones of new populations, which were identified under complex summer stress (high temperature and humidity, Pythium blight, and brown patch), and it exhibited relatively high consistency (77%) with the phenotype. We believe that with more markers obtained in the future, better efficiency is likely to be achieved in breeding for improved SST in tall fescue and possibly other species as well. Further studies that analyze the factors relating to the SCAR marker are needed.