Evaluation of Six Methods for Extraction of Total RNA from Loquat

Authors

  • Zhang LING College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Zhang ZHIKE College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Lin SHUNQUAN College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Zheng TINGTING College of Horticulture, South China Agricultural University, Guangzhou 510642
  • Yang XIANGHUI College of Horticulture, South China Agricultural University, Guangzhou 510642 (CN)

DOI:

https://doi.org/10.15835/nbha4118233

Abstract

 

Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.

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Author Biographies

Zhang LING, College of Horticulture, South China Agricultural University, Guangzhou 510642

Zhang ZHIKE, College of Horticulture, South China Agricultural University, Guangzhou 510642

Lin SHUNQUAN, College of Horticulture, South China Agricultural University, Guangzhou 510642

Zheng TINGTING, College of Horticulture, South China Agricultural University, Guangzhou 510642

Yang XIANGHUI, College of Horticulture, South China Agricultural University, Guangzhou 510642

College of Horticulture, associate professor

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Published

2013-05-28

How to Cite

LING, Z., ZHIKE, Z., SHUNQUAN, L., TINGTING, Z., & XIANGHUI, Y. (2013). Evaluation of Six Methods for Extraction of Total RNA from Loquat. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1), 313–316. https://doi.org/10.15835/nbha4118233

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Section

Research Articles
CITATION
DOI: 10.15835/nbha4118233

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