Study of Cytostatic and Cytotoxic Activity of Several Polyphenolic Extracts Obtained from Vitis vinifera

  • Ancuta NECHITA University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3 Aleea Mihail Sadoveanu, 700490 Iasi
  • Valeriu V. COTEA University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3 Aleea Mihail Sadoveanu, 700490 Iasi
  • Constantin-Bogdan NECHITA University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3 Aleea Mihail Sadoveanu, 700490 Iasi
  • Rotinberg PINCU The Institute of Biological Researches, 47 Lascăr Catargiu Street, 700107 Iasi
  • Cosmin-Teodor MIHAI „Al.I.Cuza” University of Iasi, Faculty of Biology, 22 A Blv. Carol I, 700505 Iasi
  • Cintia Lucia COLIBABA University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3 Aleea Mihail Sadoveanu, 700490 Iasi

Abstract

The study of polyphenolic extracts from skins, seeds, grape pomace and lees is justified by the huge amount of information from specialized literature, drawing attention to the many pharmacological effects of these biomolecules. The concentrated vegetal extracts obtained from seeds, skins, grape pomace and lees (yeast deposit after fermentation) were characterized according to their content of total polyphenols, anthocyanins, dry matter, ash and pH. The biological material used in the in vitro testing experiments in order to study cytostatic and cytotoxic effects were stabilized cultures of HeLa cancer cells, uncontaminated with mycoplasma and derived from a human uterine cervix carcinoma. Another action of the cytostatic substances could be exerted upon the cell proliferation process. The cell division process of the HeLa cell cultures treated with seeds by-product showed remarkable quantitative changes. The study has also evidenced a great number of dead cells in the composition of the treated HeLa cell cultures, their existence pointing out that the bioactive agent induced a major decrease of the cells viability. The obtained results in the context of the complex evaluation of the in vitro antitumoral property of the extracts, obtained from seeds, skins, grape pomace and wine lees from the ‘Arcaş’ grape variety, on HeLa cells cultures, have demonstrated the significant cytostatic and cytotoxic potential of the seeds polyphenolic biopreparate. The results obtained show that polyphenolic extracts from Vitis vinifera seeds act as cytostatic and cytotoxic agents.

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Published
2012-05-14
How to Cite
NECHITA, A., COTEA, V. V., NECHITA, C.-B., PINCU, R., MIHAI, C.-T., & COLIBABA, C. L. (2012). Study of Cytostatic and Cytotoxic Activity of Several Polyphenolic Extracts Obtained from Vitis vinifera. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 216-221. https://doi.org/10.15835/nbha4017534
Section
Research Articles