Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant

Authors

  • Afsaneh SAMADI University of Urmia, Faculty of Science, Department of Biology, Urmia (IR)
  • Jirair CARAPETIAN University of Urmia, Faculty of Science, Department of Biology, Urmia (IR)
  • Reza HEIDARI University of Urmia, Faculty of Science, Department of Biology, Urmia (IR)
  • Morad JAFARI University of Urmia, Faculty of Agriculture, Department of Agronomy and Plant Breeding, Urmia (IR)
  • Abdollah HASSANZADEH GORTTAPEH Agricultural and Natural Resources, Research Center, West Azerbaijan (IR)

DOI:

https://doi.org/10.15835/nbha4017312

Abstract

Transgenic hairy root system is a promising source of secondary metabolites in medicinal plants with high pharmaceutical value.For the first time, hairy roots were established in different explants of Linum mucronatum, an anti-cancer agent producing plant, via amikimopine type strain of Agrobacterium rhizogenes, ‘A13’. The percentage of hairy root induction varied from 0 to 60% depended onthe explants and hypocotyl (including cotyledonary node) explants were found to be highly susceptible to A. rhizogenes infection withthe highest (60%) rate of hairy root induction. four different Murashige and Skoog (MS)-based liquid culture media were used for wellestablishment of hairy roots. Hairy root growth medium D (HRGM-D) containing hormone-free MS basal medium with an extra oneday pre-incubation period at 35°C was found to be more efficient for profuse growth (fresh weight; 8500 mg per 25 ml culture medium)of hairy roots. Hairy root system presented in this study may offer a suitable platform for optimization and production of satisfactorylevel of aryltetralin lignans like podophyllotoxin and its derivatives from L. mucronatum.

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Author Biographies

Afsaneh SAMADI, University of Urmia, Faculty of Science, Department of Biology, Urmia

Department of Biology, PhD student

Jirair CARAPETIAN, University of Urmia, Faculty of Science, Department of Biology, Urmia

Department of Biology, Professor

Reza HEIDARI, University of Urmia, Faculty of Science, Department of Biology, Urmia

Department of Biology, Professor

Morad JAFARI, University of Urmia, Faculty of Agriculture, Department of Agronomy and Plant Breeding, Urmia

Department of Agronomy and Plant Breeding, Assistant Professor

Abdollah HASSANZADEH GORTTAPEH, Agricultural and Natural Resources, Research Center, West Azerbaijan

Agricultural and Natural Resources, Research Center; Assistant Professor

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Published

2012-05-14

How to Cite

SAMADI, A., CARAPETIAN, J., HEIDARI, R., JAFARI, M., & HASSANZADEH GORTTAPEH, A. (2012). Hairy Root Induction in Linum mucronatum ssp. mucronatum, an Anti-Tumor Lignans Producing Plant. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 125–131. https://doi.org/10.15835/nbha4017312

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Section

Research Articles
CITATION
DOI: 10.15835/nbha4017312