Effect of Gamma Radiation on Protein Profile, Protein Fraction and Solubility’s of Three Oil Seeds: Soybean, Peanut and Sesame

Authors

  • Abd El-Moneim M.R. AFIFY Cairo University, Faculty of Agriculture, Department of Biochemistry, P. Box 12613, Gamma st, Giza, Cairo (EG)
  • Mohamed M. RASHED Cairo University, Faculty of Agriculture, Department of Biochemistry, P. Box 12613, Gamma st, Giza, Cairo (EG)
  • Ebtesam A. MAHMOUD Cairo University, Faculty of Agriculture, Department of Biochemistry, P. Box 12613, Gamma st, Giza, Cairo (EG)
  • Hossam Saad EL-BELTAGI National Research Centre, Pharmaceutical Sciences Div., Medicinal and Aromatic Plants Dept., Dokki,12311, Cairo (EG)

DOI:

https://doi.org/10.15835/nbha3926252

Abstract

The proteins properties of three oil seeds (soybean, peanut and sesame) were investigated following γ-irradiation (0.0, 0.5, 1.0, 2.0, 3.0, 5.0 and 7.5 KGy). The effect of γ-radiation on total protein solubility, albumin, globulin and SDS-ME fractions were studied using SDS-polyacrylamide gel electrophoresis. The results showed that solubility of total protein were decreased and reached to the maximum decrease using irradiation dose of 7.5 KGy compared to control. The interesting phenomena are that albumin and globulin fractions decreased in its solubility while the SDS-ME fraction increased. These phenomena may be due to the effect of gamma radiation on the protein, which may dissociate this fraction to small subunits, and rearrangement to form a complex protein even high or small molecular weight proteins solubilized only in SDS-ME fraction. The changes in protein profile were depended even on radiation dose and on the nature of oil seeds; soybean, peanut and sesame.

Author Biographies

Abd El-Moneim M.R. AFIFY, Cairo University, Faculty of Agriculture, Department of Biochemistry, P. Box 12613, Gamma st, Giza, Cairo

Cairo University, Faculty of Agriculture, Department of Biochemistry, P. Box 12613, Gamma st, Giza, Cairo

Hossam Saad EL-BELTAGI, National Research Centre, Pharmaceutical Sciences Div., Medicinal and Aromatic Plants Dept., Dokki,12311, Cairo

Department of Biochemistry, Faculty of Agriculture, Cairo University

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Published

2011-11-21

How to Cite

AFIFY, A. E.-M. M., RASHED, M. M., MAHMOUD, E. A., & EL-BELTAGI, H. S. (2011). Effect of Gamma Radiation on Protein Profile, Protein Fraction and Solubility’s of Three Oil Seeds: Soybean, Peanut and Sesame. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), 90–98. https://doi.org/10.15835/nbha3926252

Issue

Vol. 39 No. 2 (2011)

Section

Research Articles
CITATION
DOI: 10.15835/nbha3926252

License

Copyright (c) 2011 Abd El-Moneim M.R. AFIFY, Mohamed M. RASHED, Ebtesam A. MAHMOUD, Hossam Saad EL-BELTAGI

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).

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