A Simplified Analysis of Different Escherichia coli Strains by Using RAPD Technique
AbstractCharacterization of different strains of Escherichia coli were analyzed for which samples were collected from different areas of Bangalore and cultured in MacConkey agar. Strains were finally isolated and their biochemical characterization was done for their proper identification, extraction of the nuclear DNA and its purity was tested. Quality testing with the help of spectrophotometer has been carried out and finally the application of a random amplified polymorphic DNA (RAPD) analysis for molecular genetic typing of pathogenic Escherichia coli strains was performed. The RAPD technique was shown to be highly reproducible. Stable banding patterns with a high discriminatory capacity were obtained using two different primers. Overall, 06 E. coli strains were analyzed with the RAPD technique. The RAPD analysis showed that the E. coli strains isolated from different locality in Bangalore (India) could be grouped into different RAPD types by using these two different primer sets. Most of these different E. coli RAPD types were not geographically restricted. There was, as expected, a tendency of higher genetic relationship among E. coli strains isolated from the same locality, which was finally obtained, while some other strains differ from each other due to the environmental impact. In conclusion it may be suggested that the RAPD technique may provide a rapid, low cost, simple and powerful tool to study the clonal epidemiology of rapid E. coli infections. RAPD technique also helps in identifying phylogenic relationship among species.
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