In vitro Shoot Development from Three Different Nodes of Cotton (Gossypium hirsutum L.)

Abstract

In plant tissue culture studies, obtaining new plantlets from many different parts of a plant is a very important feature with direct or indirect ways and all nodes are considerable sources, although they show different regeneration capacities from one species to another. In this research, in vitro direct shoot developments from different nodes of cotton (Gossypium hirsutum L. var. Nazilli 84S) were comparatively studied. Cotyledonary nodes, first and second leaf nodes together with hypocotyl, shoot and epicotyl pieces from 35-day-old in vitro grown plants were cultured on MS media supplemented with 0.1 mg/l KIN (kinetin). Cultured seeds and explants were kept at growth chamber with a photoperiod for 16 hours light (7500 lx) and 8 hours dark, at 25 oC and 70 % humidity. A rapid in vitro shoot development was obtained from all three types of explants and no significant differences among young plants were observed. Shoot development was maximum 74.2 % in cotyledonary nodes, followed by 60.7 % and 41.6 %, respectively for first and second leaf nodes. All these shoots were capable of rooting in Woody Plant Medium (WPM), supplemented with 1 mg/l IBA (indole-3-butyric acid), and establishing in soil after 15-30 days.