Comparison of pepper accessions acting as rootstocks:  A case with low P inputs

Maria J. PEREZ-CORDOBA; Mónica DIEZ-DIAZ; Ana de LUIS; Vicente CASTELL-ZEISING; Adrian RODRIGUEZ-BURRUEZO; Ana  FITA

doi:10.15835/nbha52213812

Authors

Maria J. PEREZ-CORDOBA Universidad Católica de Valencia San Vicente Mártir, Escuela de Doctorado, C/Guillem de Castro 65, 46008 Valencia (ES)
Mónica DIEZ-DIAZ Universidad Católica de Valencia San Vicente Mártir, Facultad de Veterinaria y Ciencias Experimentales, Departamento de Biotecnología, C/Guillem de Castro 94, 46001 Valencia (ES)
Ana de LUIS Universidad Católica de Valencia San Vicente Mártir, Facultad de Veterinaria y Ciencias Experimentales, Departamento de Biotecnología, C/Guillem de Castro 94, 46001 Valencia (ES)
Vicente CASTELL-ZEISING Universitat Politècnica de València, Departamento de Producción vegetal, Camino de Vera s/n, 46022 València (ES)
Adrian RODRIGUEZ-BURRUEZO Universitat Politècnica de València, Instituto de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Camino de Vera s/n, 46022 València (ES)
Ana FITA Universitat Politècnica de València, Instituto de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Camino de Vera s/n, 46022 València (ES)

DOI:

https://doi.org/10.15835/nbha52213812

Keywords:

breeding, grafting, incompatibility, low input systems, shovelomics, root architecture

Abstract

Fertilization is essential for maintaining production in agriculture. Yet, in too high quantity it causes high impact in environment and in farmers economy. This is especially true in the case of phosphorus (P) fertilization. Finding genotypes adapted to low P conditions may help to reduce the problem. P efficiency depends to some extend on the ability of the roots to acquire this mineral, therefore using efficient rootstocks would provide a higher acquisition of P, maintaining the good characteristics of the scion varieties. In this study, twenty diverse pepper accessions (Capsicum annuum L.) have been evaluated as possible rootstocks to increase P acquisition and yield in pepper under no P fertilization. Plant production, biomass, P content and physiological phosphorous use efficiency were evaluated for ‘Lobo’ variety grafted onto different rootstocks. In addition, root traits, measured manually (shovelomics) and semi-automatically (Winrhizo) were studied. The results showed a great diversity in the root traits for the studied accessions. These root traits changed significantly when the accessions act as rootstock, indicating great rootstock/scion interactions. In general, all the rootstocks adapted their root size and shape to that displayed by ‘Lobo’ root system. Some accessions seemed to have some incompatibility whereas some others enhanced the scion performance. It was possible to identify some genotypes suitable to act as rootstocks for pepper with good performance under low P conditions. Root length, root weight, branching, and root angle were identified as key root traits for plant growth and P acquisition under low P conditions.

References

Alaguero-Cordovilla A, Gran-Gómez FJ, Tormos-Moltó S, Pérez-Pérez JM (2018). Morphological characterization of root system architecture in diverse tomato genotypes during early growth. International Journal of Molecular Sciences 19(12):3888. https://doi.org/10.3390/ijms19123888

Arifuzzaman M, Oladzadabbasabadi A, McClean P, Rahman M (2019). Shovelomics for phenotyping root architectural traits of rapeseed/canola (Brassica napus L.) and genome-wide association mapping. Molecular Genetics and Genomics 294:985-1000. https://doi.org/10.1007/s00438-019-01563-x

Arsenault JL, Poulcur S, Messier C, Guay R (1995). WinRHlZO™, a root-measuring system with a unique overlap correction method. HortScience 30(4):906D-906. https://doi.org/10.21273/HORTSCI.30.4.906D

Batjes NH (2011). Global distribution of soil phosphorus retention potential (No. 2011/06). ISRIC-World Soil Information.

Bindraban PS, Dimkpa C, Nagarajan L, Roy A, Rabbinge R (2015). Revisiting fertilisers and fertilisation strategies for improved nutrient uptake by plants. Biology and Fertility of Soils 51(8):897-911. https://doi.org/10.1007/s00374-015-1039-7

Brownlie WJ, Sutton MA, Heal KV, Reay DS, Spears B (2022). Our phosphorus future: towards global phosphorus sustainability. Published by UK Centre for Ecology & Hydrology (UKCEH), on behalf of the Our Phosphorus Future Network.

Camalle MD, Sikron N, Zurgil U, Khadka J, Pivonia S, Pěnčík A, ... Tel-Zur N (2021). Does scion–rootstock compatibility modulate photoassimilate and hormone trafficking through the graft junction in melon–pumpkin graft combinations? Plant Science 306:110852. https://doi.org/10.1016/j.plantsci.2021.110852

Chen Z, Wang L, Cardoso JA, Zhu S, Liu G, Rao IM, Lin Y (2023). Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes. Frontiers in Plant Science 14:1094157. https://doi.org/10.3389/fpls.2023.1094157

Fafu W, Jianxiong W, Jiangtao L, Guoping Z, Peng X, Peng H, Wenshuai X (2021). Distribution, geology and development status of phosphate resources. Geology in China 48(1):82-101. https://dx.doi.org/10.12029/gc20210106

Fallik E, Ziv C (2020). How rootstock/scion combinations affect watermelon fruit quality after harvest? Journal of the Science of Food and Agriculture 100(8):3275-3282. https://doi.org/10.1002/jsfa.10325

FAOSTAT (2023). Retrieved 2024 March 1st from: https://www.fao.org/faostat/es/#home.

Fita A, Nuez F, Picó B (2011). Diversity in root architecture and response to P deficiency in seedlings of Cucumis melo L. Euphytica 181:323-339. https://doi.org/10.1007/s10681-011-0432-z

Fita A, Picó B, Dias RCS, Nuez F (2008). Effects of root architecture on response to melon vine decline. The Journal of Horticultural Science and Biotechnology 83(5):616-623. https://doi.org/10.1080/14620316.2008.11512432

Fita A, Pico B, Roig C, Nuez F (2007). Performance of Cucumis melo ssp. agrestis as a rootstock for melon. The Journal of Horticultural Science and Biotechnology 82(2):184-190. http://dx.doi.org/10.1080/14620316.2007.11512218

Gao ZY, Hu JA, Zhang BB, Gong B (2023). Screening and comprehensive evaluation of tomato rootstocks with high efficiency of phosphorus utilization. Scientia Agricultura Sinica 56:2761-2775. https://doi.org/10.3864/j.issn.0578-1752.2023.14.011

Gautier AT, Cochetel N, Merlin I, Hevin C, Lauvergeat V, Vivin P, ... Cookson SJ (2020). Scion genotypes exert long distance control over rootstock transcriptome responses to low phosphate in grafted grapevine. BMC Plant Biology 20:1-15. https://doi.org/10.1186/s12870-020-02578-y

Ge X, Chen X, Liu M, Wang C, Zhang Y, Wang Y, … Zhang T (2023). Toward a better understanding of phosphorus nonpoint source pollution from soil to water and the application of amendment materials: Research trends. Water 15:1531. https://doi.org/10.3390/w15081531

Howden SM, Soussana JF, Tubiello FN, Chhetri N, Dunlop M, Meinke H (2007). Adapting agriculture to climate change. Proceedings of the National Academy of Sciences 104(50):19691-19696. http://dx.doi.org/10.1073/pnas.0701890104

Kirchgesser J, Hazarika M, Bachmann-Pfabe S (2023). Phenotypic variation of root-system architecture under high P and low P conditions in potato (Solanum tuberosum L.). BMC Plant Biology 23:68. https://doi.org/10.1186/s12870-023-04070-9

Lambers H, Shane MW, Cramer MD, Pearse SJ, Veneklaas EJ (2006). Root structure and functioning for efficient acquisition of phosphorus: Matching morphological and physiological traits. Annals of Botany 98(4):693-713. https://doi.org/10.1093/aob/mcl114

Leal-Fernández C, Godoy-Hernández H, Núñez-Colín CA, Anaya-López JL, Villalobos-Reyes S, Castellanos JZ (2013). Morphological response and fruit yield of sweet pepper (Capsicum annuum L.) grafted onto different commercial rootstocks. Biological Agriculture & Horticulture 29(1):1-11. https://doi.org/10.1080/01448765.2012.746063

Louws FJ, Rivard CL, Kubota C (2010). Grafting fruiting vegetables to manage soilborne-pathogens, foliar pathogens, arthropods and weeds. Scientia Horticulturae 127:127-146. https://doi.org/10.1016/j.scienta.2010.09.023

Lynch J (2011) Root phenes for enhanced soil exploration and phosphorus acquisition: tools for future crops. Plant Physiology 156(3):1041-1049. https://doi.org/10.1104/pp.111.175414

Lynch J (2022). Harnessing root architecture to address global challenges. The Plant Journal 109(2):415-431. https://doi.org/10.1111/tpj.15560

Lynch JP (2019) Root phenotypes for improved nutrient capture: an underexploited opportunity for global agriculture. New Phytologist 223:548-564. https://doi.org/10.1111/nph.15738

Martínez-Andújar C, Ruiz-Lozano JM, Dodd IC, Albacete A, Pérez-Alfocea F (2017). Hormonal and nutritional features in contrasting rootstock-mediated tomato growth under low-phosphorus nutrition. Frontiers in Plant Science 8:533. https://doi.org/10.3389%2Ffpls.2017.00533

Möller K (2018). Soil fertility status and nutrient input–output flows of specialised organic cropping systems: A review. Nutrient Cycling in Agroecosystems 112(2):147-164. https://link.springer.com/article/10.1007/s10705-018-9946-2

Morales-Manzo II, Ribes-Moya AM, Pallotti C, Jimenez-Belenguer A, Moro CP, Raigón MD, ... Fita A (2023). Root–soil interactions for pepper accessions grown under organic and conventional farming. Plants 12(9):1873. https://doi.org/10.3390/plants12091873

Nakhforoosh A, Grausgruber H, Kaul HP, Bodner G (2014). Wheat root diversity and root functional characterization. Plant and Soil 380:211-229. https://doi.org/10.1007/s11104-014-2082-0

Penuelas J, Coello F, Sardans J (2023). A better use of fertilizers is needed for global food security and environmental sustainability. Agriculture & Food Security 12(1):1-9. https://doi.org/10.1186/s40066-023-00409-5

Pereira-Dias L, Gil-Villar D, Castell-Zeising V, Quiñones A, Calatayud Á, Rodríguez-Burruezo A, Fita A (2020). Main root adaptations in pepper germplasm (Capsicum spp.) to phosphorus low-input conditions. Agronomy 10(5):637. https://doi.org/10.3390/agronomy10050637

Perez-Cordoba MJ, Rodriguez Burruezo A, Diez Diaz M, De Luis A, Fita A (2018). Crosstalk scion-rootstock modifies root architecture in pepper rootstocks. Journal of Biotechnology 280:S86. http://dx.doi.org/10.1016/j.jbiotec.2018.06.284

Ribeiro CAG, de Sousa Tinoco SM, de Souza VF, Negri BF, Gault CM, Pastina MM, ... Guimaraes CT (2023). Genome-wide association study for root morphology and phosphorus acquisition efficiency in diverse maize panels. International Journal of Molecular Sciences 24(7):6233. https://doi.org/10.3390/ijms24076233

Ropokis A, Ntatsi G, Kittas C, Katsoulas N, Savvas D (2019). Effects of temperature and grafting on yield, nutrient uptake, and water use efficiency of a hydroponic sweet pepper crop. Agronomy 9:110. https://doi.org/10.3390/agronomy9020110

Rouphael Y, Venema JH, Edelstein M, Savvas D, Colla G, Ntatsi G, Ben-Hur M, Kumar P, Schwarz D (2017). Grafting as a tool for tolerance of abiotic stress. In: Colla G, Pérez-Alfocea F, Schwarz D (Eds). Vegetable Grafting: Principles and Practices. CABI: Oxfordshire, UK.

Statista (2024). US Geological Survey. (January 29, 2024). Reserves of phosphate rock worldwide in 2023, by country (in million metric tons) [Graph]. In: Statista. Retrieved 2024 April 3 from: https://www.statista.com/statistics/681747/phosphate-rock-reserves-by-country/

Trachsel S, Kaeppler SM, Brown KM, Lynch JP (2011). Shovelomics: high throughput phenotyping of maize (Zea mays L.) root architecture in the field. Plant and Soil 341:75-87. http://dx.doi.org/10.1007/s11104-010-0623-8

Tsaballa A, Xanthopoulou A, Madesis P, Tsaftaris A, Nianiou-Obeidat I (2021). Vegetable grafting from a molecular point of view: the involvement of epigenetics in rootstock-scion interactions. Frontiers in Plant Science 11:621999. https://doi.org/10.3389/fpls.2020.621999

van de Wiel CC, van der Linden CG, Scholten OE (2016). Improving phosphorus use efficiency in agriculture: opportunities for breeding. Euphytica 207:1-22. https://doi.org/10.1007/s10681-015-1572-3

Wen Z, Li H, Shen Q, Tang X, Xiong C, Li H, … Shen J (2019). Tradeoffs among root morphology, exudation and mycorrhizal symbioses for phosphorus-acquisition strategies of 16 crop species. New Phytologist 223(2):882-895. https://doi.org/10.1111/nph.15833.

Williamson LC, Ribrioux SPCP, Fitter AH, Leyser HMO (2001). Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiology 126:875-882. https://doi.org/10.1104/pp.126.2.875

Ye H, Roorkiwal M, Valliyodan B, Zhou L, Chen P, Varshney RK, Nguyen HT (2018). Genetic diversity of root system architecture in response to drought stress in grain legumes. Journal of Experimental Botany 69(13):3267-3277. https://doi.org/10.1093/jxb/ery082.

Zhang C, Zhang S, Li C, Li Q, Zhang J, Wang J, ... Guo L (2022). Long-term fertilization altered microbial community structure in an aeolian sandy soil in northeast China. Frontiers in Microbiology 13:979759. https://doi.org/10.3389/fmicb.2022.979759.