ACC-Deaminase producing Pseudomonas putida RT12 inoculation: A promising strategy for improving Brassica juncea tolerance to salinity stress


  • Wardah A. ALHOQAIL Majmaah University, College of Science in Zulfi, Department of Biology, Al-Majmaah 11952 (SA)



Acc-deaminase, antioxidants, Brassica juncea, Pseudomonas putida RT12, PGPR, sality stress


An important abiotic stressor that hinders plant growth, nutrient uptake, and global agricultural productivity is soil salinity. Among the different strategies to overcome the issue of salinity in agriculture sector, plant growth-promoting rhizobacteria (PGPR) have gained recognition as promising beneficial microbes that can improve plants’ response to biotic and abiotic stressors. The salinity tolerance and traits that promote plant growth of eight PGPR strains (RT1, RT2, RT3, RT4, RT5, RT7, and RT12) were evaluated in this study. During screening, one strain, RT12, had the highest plant growth-promoting activity and salt tolerance in the group. The strain when subjected to NaCl stress showed quantitative ACC-deaminase activity, in the presence of NaCl at various concentrations, demonstrating extraordinary tolerance to salt stress by withstanding doses of up to 3M NaCl. In order to further investigate the effects of salt stress on Brassica juncea (mustard), RT12, which was identified as Pseudomonas putida using 16s RNA sequencing, was inoculated. Two salt treatments (100 and 150 mM) were applied to the mustard variety ‘Mingora’ in a greenhouse. The results revealed that through ACC utilization, PGPR directly induced plant growth in salt-stressed mustard plants by lowering excess ethylene production. All plant parameters were negatively impacted by an increase in NaCl concentration in uninoculated plants. However, P. putida RT12 inoculation enhanced all growth parameters, antioxidant production, total soluble sugar (TSS), total protein (TP), proline, relative water content (RWC), chlorophyll contents, and nutrient uptake in salt-treated plants. The inoculation with P. putida also caused a marked decline in Na+ uptake and an increase in K+ uptake in the shoot. By maintaining a greater K+/Na+ ratio in the tissues of RT12-inoculated plants compared to controls, this change in ion uptake helped to maintain nutritional balance of the plants. The findings suggest that inoculating plants with ACC deaminase-producing PGPR, such as P. putida RT12, may boost growth and stress resistance.


Aazami MA, Rasouli F, Ebrahimzadeh A (2021). Oxidative damage, antioxidant mechanism and gene expression in tomato responding to salinity stress under in vitro conditions and application of iron and zinc oxide nanoparticles on callus induction and plant regeneration. BMC Plant Biology 21(1):597.

Ahmad I, Akhtar MJ, Mehmood S, Akhter K, Tahir M, Saeed MF, ... Hussain S (2018). Combined application of compost and Bacillus sp. CIK-512 ameliorated the lead toxicity in radish by regulating the homeostasis of antioxidants and lead. Ecotoxicology and Environmental Safety 148:805-812.

Akbar A, Han B, Khan AH, Feng C, Ullah A, Khan AS, ... Yang X (2022). A transcriptomic study reveals salt stress alleviation in cotton plants upon salt tolerant PGPR inoculation. Environmental and Experimental Botany 200:104928.

Alemneh AA, Zhou Y, Ryder MH, Denton MD (2021). Large-scale screening of rhizobacteria to enhance the chickpea-Mesorhizobium symbiosis using a plant-based strategy. Rhizosphere 18:100361.

Ali F, Kainat Khan W, Khan A, Jan AU, Amin M, Musarella CM (2023). Effect of different concentrations of pyridoxine on physiological indices of Brassica rapa varieties. Vegetos 1-10.

Alinia M, Kazemeini SA, Dadkhodaie A, Sepehri M, Mahjenabadi VAJ, Amjad SF, ... Abdelhafez AA (2022). Co-application of ACC deaminase-producing rhizobial bacteria and melatonin improves salt tolerance in common bean (Phaseolus vulgaris L.) through ion homeostasis. Scientific Reports 12(1):22105.

Arif I, Batool M, Schenk PM (2020). Plant microbiome engineering: expected benefits for improved crop growth and resilience. Trends in Biotechnology 38(12):1385-1396.

Ayuso-Calles M, Flores-Félix JD, Rivas R (2021). Overview of the role of rhizobacteria in plant salt stress tolerance. Agronomy 11(9):1759.

Azeem MA, Shah FH, Ullah A, Ali K, Jones DA, Khan MEH, Ashraf A (2022). Biochemical characterization of halotolerant Bacillus safensis PM22 and its potential to enhance growth of maize under salinity stress. Plants 11(13):1721.

Bhadrecha P, Singh S, Dwibedi V (2023). A plant’s major strength in rhizosphere: the plant growth promoting rhizobacteria. Archives of Microbiology 205(5):165.

Borah P, Gogoi N, Asad SA, Rabha AJ, Farooq M (2023). An insight into plant growth-promoting rhizobacteria-mediated mitigation of stresses in plant. Journal of Plant Growth Regulation 42(5):3229-3256.

Breś W, Kleiber T, Markiewicz B, Mieloszyk E, Mieloch M (2022). The effect of NaCl stress on the response of lettuce (Lactuca sativa L.). Agronomy 12(2):244.

Choudhary M, Chandra P, Dixit B, Nehra V, Choudhary U, Choudhary S (2022). Plant growth-promoting microbes: Role and prospective in amelioration of salt stress. Communications in Soil Science and Plant Analysis 53(13):1692-1711.

Desoky E-SM, Saad AM, El-Saadony MT, Merwad A-RM, Rady MM (2020). Plant growth-promoting rhizobacteria: Potential improvement in antioxidant defense system and suppression of oxidative stress for alleviating salinity stress in Triticum aestivum (L.) plants. Biocatalysis and Agricultural Biotechnology 30:101878.

Dinesh R, Anandaraj M, Kumar A, Bini YK, Subila KP, Aravind R (2015). Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. Microbiological Research 173:34-43.

Du K, Huang J, Wang W, Zeng Y, Li X, Zhao F (2024). Monitoring low-temperature stress in winter wheat using TROPOMI solar-induced chlorophyll fluorescence. IEEE Transactions on Geoscience and Remote Sensing.

Dugasa MT, Cao F, Ibrahim W, Wu F (2019). Differences in physiological and biochemical characteristics in response to single and combined drought and salinity stresses between wheat genotypes differing in salt tolerance. Physiologia Plantarum 165(2):134-143.

Egamberdieva D, Wirth S, Bellingrath-Kimura SD, Mishra J, Arora NK (2019). Salt-tolerant plant growth promoting rhizobacteria for enhancing crop productivity of saline soils. Frontiers in Microbiology 10:2791.

Etesami H, Glick BR (2020). Halotolerant plant growth–promoting bacteria: Prospects for alleviating salinity stress in plants. Environmental and Experimental Botany 178:104124.

Etesami H, Noori F (2019). Soil salinity as a challenge for sustainable agriculture and bacterial-mediated alleviation of salinity stress in crop plants. Saline soil-based agriculture by halotolerant microorganisms 1-22.

Fadiji AE, Santoyo G, Yadav AN, Babalola OO (2022). Efforts towards overcoming drought stress in crops: Revisiting the mechanisms employed by plant growth-promoting bacteria. Frontiers in Microbiology 13:962427.

Falcinelli B, Sileoni V, Marconi O, Perretti G, Quinet M, Lutts S, Benincasa P (2017). Germination under moderate salinity increases phenolic content and antioxidant activity in rapeseed (Brassica napus var oleifera Del.) sprouts. Molecules 22(8):1377.

Farahat MG, Mahmoud MK, Youseif SH, Saleh SA, Kamel Z (2020). Alleviation of salinity stress in wheat by ACC deaminase-producing Bacillus aryabhattai EWR29 with multifarious plant growth-promoting attributes. Plant Archives 20(1):417-429.

Fatima T, Mishra I, Verma R, Arora NK (2020). Mechanisms of halotolerant plant growth promoting Alcaligenes sp. involved in salt tolerance and enhancement of the growth of rice under salinity stress. 3 Biotech 10:1-12.

Franchi E, Fusini D (2021). Plant growth‐promoting rhizobacteria (PGPR) assisted phytoremediation of inorganic and organic contaminants including amelioration of perturbed marginal soils. Handbook of Assisted and Amendment: Enhanced Sustainable Remediation Technology 477-500.

Gontia-Mishra I, Sapre S, Tiwari S (2017). Zinc solubilizing bacteria from the rhizosphere of rice as prospective modulator of zinc biofortification in rice. Rhizosphere 3:185-190.

Gupta S, Pandey S (2020). Enhanced salinity tolerance in the common bean (Phaseolus vulgaris) plants using twin ACC deaminase producing rhizobacterial inoculation. Rhizosphere 16:100241.

Haroon U, Khizar M, Liaquat F, Ali M, Akbar M, Tahir K, ... Munis MFH (2021). Halotolerant plant growth-promoting rhizobacteria induce salinity tolerance in wheat by enhancing the expression of SOS genes. Journal of Plant Growth Regulation 1-14.

Hasanuzzaman M, Bhuyan MB, Zulfiqar F, Raza A, Mohsin SM, Mahmud JA, ... Fotopoulos V (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants 9(8):681.

Hasimuna OJ, Maulu S, Nawanzi K, Lundu B, Mphande J, Phiri CJ, ... Chibesa M (2023). Integrated agriculture-aquaculture as an alternative to improving small-scale fish production in Zambia. Frontiers in Sustainable Food Systems 7:1161121.

Hayat K, Menhas S, Bundschuh J, Zhou P, Niazi NK, Amna AH, ... Wang J (2020). Plant growth promotion and enhanced uptake of Cd by combinatorial application of Bacillus pumilus and EDTA on Zea mays L. International Journal of Phytoremediation 22(13):1372-1384.

Hjort K, Presti I, Elväng A, Marinelli F, Sjöling S (2014). Bacterial chitinase with phytopathogen control capacity from suppressive soil revealed by functional metagenomics. Applied Microbiology and Biotechnology 98:2819-2828.

Hossain MA, Nakano Y, Asada K (1984). Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant and Cell Physiology 25(3):385-395.

Hsu S-H, Shen M-W, Chen J-C, Lur H-S, Liu C-T (2021). The photosynthetic bacterium Rhodopseudomonas palustris strain PS3 exerts plant growth-promoting effects by stimulating nitrogen uptake and elevating auxin levels in expanding leaves. Frontiers in Plant Science 12:573634.

Javed H, Aneela R, Qureshi A, Javed K, Mujeeb F, Fraza I, . . . Aftab M (2020). Isolation, characterization and screening of PGPR capable of providing relief in salinity stress. Eurasian Journal of Soil Science 9(2):85-91.

Johnson R, Vishwakarma K, Hossen MS, Kumar V, Shackira A, Puthur JT, ... Hasanuzzaman M (2022). Potassium in plants: Growth regulation, signaling, and environmental stress tolerance. Plant Physiology and Biochemistry 172:56-69.

Kerchev PI, Van Breusegem F (2022). Improving oxidative stress resilience in plants. The Plant Journal 109(2):359-372.

Khan AA, AlHaithloul HAS, Alghanem SM, Senousy HH, Alhoqail WA, Shi F, ... Soliman MH (2023). Petrosolinum crispum under stressful conditions medicinal plant responses to stressful conditions. CRC Press, pp 299-310.

Khan AA, Ali F, Ihsan M, Hayat K, Nabi G (2015). Ethnobotanical study of the medicinal plants of Tehsil Charbagh, district Swat, Khyber Pakhtunkhwa, Pakistan. American Eurasian Journal of Agriculture and Environmental Sciences 15:1464-1474.

Khan AA, Wang T, Hussain T, Amna Ali F, Shi F, ... Mehmood S (2021). Halotolerant-Koccuria rhizophila (14asp)-induced amendment of salt stress in pea plants by limiting Na+ uptake and elevating production of antioxidants. Agronomy 11(10):1907.

Khan AA, Wang T, Nisa ZU, Alnusairi GS, Shi F (2022). Insights into cadmium-induced morphophysiological disorders in Althea rosea Cavan and its phytoremediation through the exogeneous citric acid. Agronomy 12(11):2776.

Khan A, Zhao XQ, Javed MT, Khan KS, Bano A, Shen RF, Masood S (2016). Bacillus pumilus enhances tolerance in rice (Oryza sativa L.) to combined stresses of NaCl and high boron due to limited uptake of Na+. Environmental and Experimental Botany 124:120-129.

Khan MA, Hamayun M, Asaf S, Khan M, Yun B-W, Kang S-M, Lee I-J (2021). Rhizospheric Bacillus spp. rescues plant growth under salinity stress via regulating gene expression, endogenous hormones, and antioxidant system of Oryza sativa L. Frontiers in Plant Science 12:665590.

Khan MA, Sahile AA, Jan R, Asaf S, Hamayun M, Imran M, ... Lee I-J (2021). Halotolerant bacteria mitigate the effects of salinity stress on soybean growth by regulating secondary metabolites and molecular responses. BMC Plant Biology 21(1):1-15.

Khan N, Bano A (2016). Role of plant growth promoting rhizobacteria and Ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation. International Journal of Phytoremediatio 18(3):211-221.

Khan N, Bano A, Rahman MA, Guo J, Kang Z, Babar MA (2019). Comparative physiological and metabolic analysis reveals a complex mechanism involved in drought tolerance in chickpea (Cicer arietinum L.) induced by PGPR and PGRs. Scientific Reports 9(1):2097.

Khan V, Umar S, Iqbal N (2023). Palliating salt stress in mustard through plant-Growth-Promoting rhizobacteria: Regulation of secondary metabolites, osmolytes, antioxidative enzymes and stress ethylene. Plants 12(4):705.

Khan W, El-Shehawi AM, Ali F, Ali M, Alqurashi M, Althaqafi MM, Alharthi SB (2023). A genome-wide identification and expression pattern of LMCO gene family from turnip (Brassica rapa L.) under various abiotic stresses. Plants 12(9):1904.

Khedher SB, Mejdoub-Trabelsi B, Tounsi S (2021). Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control 152:104444.

Kul R (2022). Integrated application of plant growth promoting rhizobacteria and biochar improves salt tolerance in eggplant seedlings. Turkish Journal of Agriculture and Forestry 46(5):677-702.

Kumar A, Singh S, Gaurav AK, Srivastava S, Verma JP (2020). Plant growth-promoting bacteria: biological tools for the mitigation of salinity stress in plants. Frontiers in Microbiology 11:1216.

Lalay G, Ullah S, Ahmed I (2022). Physiological and biochemical responses of Brassica napus L. to drought‐induced stress by the application of biochar and Plant Growth Promoting Rhizobacteria. Microscopy Research and Technique 85(4):1267-1281.

Li Y, Mo X, Xiong J, Huang K, Zheng M, Jiang Q, ... Jiang C (2023). Deciphering the probiotic properties and safety assessment of a novel multi-stress-tolerant aromatic yeast Pichia kudriavzevii HJ2 from marine mangroves. Food Bioscience 56:103248.

Lim H-S, Yoo E-J, Choi M-R (2000). Changes of physiological activity of mustard leaf during its fermentation period. Journal of Microbiology and Biotechnology 10(1):43-47.

Mandal K, Hati K, Misra A, Bandyopadhyay K (2010). Root biomass, crop response and water-yield relationship of mustard (Brassica juncea L.) grown under combinations of irrigation and nutrient application. Irrigation Science 28:271-280.

Mandal S, Raju R, Kumar A, Kumar P, Sharma P (2018). Current status of research, technology response and policy need of salt-affected soils in India–a review Journal of the Indian Society of Coastal Agricultural Research 36:40-53.

Maqbool S, Amna A, Mehmood S, Suhaib M, Sultan T, Munis MFH (2021). Interaction of acc deaminase and antioxidant enzymes to induce drought tolerance in Enterobacter cloacae 2wc2 inoculated maize genotypes. Pakistan Journal of Botany 53(3).

Masmoudi F, Tounsi S, Dunlap CA, Trigui M (2021). Halotolerant Bacillus spizizenii FMH45 promoting growth, physiological, and antioxidant parameters of tomato plants exposed to salt stress. Plant Cell Reports 40(7):1199-1213.

Mehmood S, Khan AA, Shi F, Tahir M, Sultan T, Munis MFH, ... Chaudhary HJ (2021). Alleviation of salt stress in wheat seedlings via multifunctional Bacillus aryabhattai PM34: An in-vitro study. Sustainability 13(14):8030.

Mo X, Zhou M, Li Y, Yu L, Bai H, Shen P, ... Bu R (2023). Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis. Food Science and Human Wellness.

Mulani R, Mehta K, Saraf M, Goswami D (2021). Decoding the mojo of plant-growth-promoting microbiomes. Physiological and Molecular Plant Pathology 115:101687.

Nadeem SM, Ahmad M, Naveed M, Imran M, Zahir ZA, Crowley DE (2016). Relationship between in vitro characterization and comparative efficacy of plant growth-promoting rhizobacteria for improving cucumber salt tolerance. Archives of Microbiology 198:379-387.

Naing AH, Maung TT, Kim CK (2021). The ACC deaminase‐producing plant growth‐promoting bacteria: influences of bacterial strains and ACC deaminase activities in plant tolerance to abiotic stress. Physiologia Plantarum 173(4):1992-2012.

Neshat M, Abbasi A, Hosseinzadeh A, Sarikhani MR, Dadashi Chavan D, Rasoulnia A (2022). Plant growth promoting bacteria (PGPR) induce antioxidant tolerance against salinity stress through biochemical and physiological mechanisms. Physiology and Molecular Biology of Plants 28(2):347-361.

Nisha Batra N, Sharma V, Sharma R, Sharma A (2023). Abiotic stress responses and strategies of microbes mediated mitigation for sustainable agriculture. Vegetos 36(1):20-27.

Noreen S, Ashraf M (2008). Alleviation of adverse effects of salt stress on sunflower (Helianthus annuus L.) by exogenous application of salicylic acid: growth and photosynthesis. Pakistan Journal of Botany 40(4):1657-1663.

Panagos P, Borrelli P, Robinson D (2020). FAO calls for actions to reduce global soil erosion. Mitigation and Adaptation Strategies for Global Change 25:789-790.

Parmar P, Sindhu S (2013). Potassium solubilization by rhizosphere bacteria: influence of nutritional and environmental conditions. Journal of Microbiology Research 3(1):25-31.

Penrose DM, Glick BR (2003). Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria. Physiologia Plantarum 118(1):10-15.

Phour M, Sindhu SS (2022). Mitigating abiotic stress: microbiome engineering for improving agricultural production and environmental sustainability. Planta 256(5):85.

Ravelo-Ortega G, Raya-González J, López-Bucio J (2023). Compounds from rhizosphere microbes that promote plant growth. Current Opinion in Plant Biology 73:102336.

Shah F, Wu W (2019). Soil and crop management strategies to ensure higher crop productivity within sustainable environments. Sustainability 11(5):1485.

Shahid M, Ameen F, Maheshwari HS, Ahmed B, AlNadhari S, Khan MS (2021). Colonization of Vigna radiata by a halotolerant bacterium Kosakonia sacchari improves the ionic balance, stressor metabolites, antioxidant status and yield under NaCl stress. Applied Soil Ecology 158:103809.

Shahid M, Zeyad MT, Syed A, Singh UB, Mohamed A, Bahkali AH, ... Pichtel J (2022). Stress-tolerant endophytic isolate Priestia aryabhattai BPR-9 modulates physio-biochemical mechanisms in wheat (Triticum aestivum L.) for enhanced salt tolerance. International Journal of Environmental Research and Public Health 19(17):10883.

Singh RP, Jha PN (2016). The multifarious PGPR Serratia marcescens CDP-13 augments induced systemic resistance and enhanced salinity tolerance of wheat (Triticum aestivum L.). PLos One 11(6):e0155026.

Singh RP, Pandey DM, Jha PN, Ma Y (2022). ACC deaminase producing rhizobacterium Enterobacter cloacae ZNP-4 enhance abiotic stress tolerance in wheat plant. PloS One 17(5):e0267127.

Singh S, Chanotiya CS, Singh A, Vajpayee P, Kalra A (2023). Role of ACC-deaminase synthesizing Trichoderma harzianum and plant growth-promoting bacteria in reducing salt-stress in Ocimum sanctum. Physiology and Molecular Biology of Plants 1-14.

Singh VK, Singh BD, Kumar A, Maurya S, Krishnan SG, Vinod KK, ... Singh AK (2018). Marker-assisted introgression of Saltol QTL enhances seedling stage salt tolerance in the rice variety “Pusa Basmati 1”. International Journal of Genomics.

Stegelmeier AA, Rose DM, Joris BR, Glick BR (2022). The use of PGPB to promote plant hydroponic growth. Plants 11(20):2783.

Sunita K, Mishra I, Mishra J, Prakash J, Arora NK (2020). Secondary metabolites from halotolerant plant growth promoting rhizobacteria for ameliorating salinity stress in plants. Frontiers in Microbiology 11:567768.

Tahir M, Khalid U, Khan MB, Shahid M, Ahmad I, Akram M, ... Naeem MA (2019). Auxin and 1-Aminocyclopropane-1-carboxylate deaminase activity exhibiting rhizobacteria improved maize quality and productivity under drought conditions. International Journal of Agricultural Biology 21:943-954.

Tiwar, S, Prasad V, Lata C (2019). Bacillus: Plant growth promoting bacteria for sustainable agriculture and environment New and future developments in microbial biotechnology and bioengineering (pp. 43-55): Elsevier.

Ullah A, Bano A (2021). Modulation of secondary metabolites: a halotolerance strategy of plant growth promoting rhizobacteria against sodium chloride stress. Current Microbiology 78:4050-4059.

Wang G, Zhang L, Zhang S, Li B, Li J, Wang X, ... Ji J (2023). The combined use of a plant growth promoting Bacillus sp. strain and GABA promotes the growth of rice under salt stress by regulating antioxidant enzyme system, enhancing photosynthesis and improving soil enzyme activities. Microbiological Research 266:127225.

Wang X, Cai D, Ji M, Chen Z, Yao L, Han H (2022). Isolation of heavy metal-immobilizing and plant growth-promoting bacteria and their potential in reducing Cd and Pb uptake in water spinach. Science of the Total Environment 819:153242.

Wang X, Tian Z, Xi Y, Guo Y (2022). Identification of endophytic fungi with ACC deaminase-producing isolated from halophyte Kosteletzkya virginica. Plant Signaling & Behavior 17(1):2152224.

Wani AS, Ahmad A, Hayat S, Tahir I (2019). Epibrassinolide and proline alleviate the photosynthetic and yield inhibition under salt stress by acting on antioxidant system in mustard. Plant Physiology and Biochemistry 135:385-394.

Wei B, Yu J, Cao Z, Meng M, Yang L, Chen Q (2020). The availability and accumulation of heavy metals in greenhouse soils associated with intensive fertilizer application. International Journal of Environmental Research and Public Health 17(15):5359.

Zainab N, Amna Khan AA, Azeem MA, Ali B, Wang T, ... Hashem M (2021). PGPR-mediated plant growth attributes and metal extraction ability of Sesbania sesban L. in industrially contaminated soils. Agronomy 11(9):1820.

Zaman M, Shahid SA, Heng L (2018). Guideline for salinity assessment, mitigation and adaptation using nuclear and related techniques: Springer Nature.

Zeeshan M, Lu M, Sehar S, Holford P, Wu F (2020). Comparison of biochemical, anatomical, morphological, and physiological responses to salinity stress in wheat and barley genotypes deferring in salinity tolerance. Agronomy 10(1):127.

Zeng Q, Ding X, Wang J, Han X, Iqbal HM, Bilal M (2022). Insight into soil nitrogen and phosphorus availability and agricultural sustainability by plant growth-promoting rhizobacteria. Environmental Science and Pollution Research 29(30):45089-45106.




How to Cite

ALHOQAIL, W. A. (2024). ACC-Deaminase producing Pseudomonas putida RT12 inoculation: A promising strategy for improving Brassica juncea tolerance to salinity stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13550.



Research Articles
DOI: 10.15835/nbha52113550