Phytochemical profile, antioxidant capacity and wound healing potential of Viscum album L. growing on Robinia pseudoacacia L.


  • Simona I. VICAS University of Oradea, Faculty of Environmental Protection, 26 Gen. Magheru st., Oradea 410048; University of Oradea, Doctoral School of Biomedical Science, 1 Universitatii st., Oradea, 410087 (RO)
  • Adriana R. MEMETE University of Oradea, Faculty of Environmental Protection, 26 Gen. Magheru st., Oradea 410048 (RO)
  • Eva KLESZKEN University of Oradea, Doctoral School of Biomedical Science, 1 Universitatii st., Oradea, 410087 (RO)
  • Florina MIERE (GROZA) University of Oradea, Faculty of Medicine and Pharmacy, 10 Piaţa 1 Decembrie st., Oradea, 410068 (RO)
  • Mariana GANEA University of Oradea, Faculty of Medicine and Pharmacy, 10 Piaţa 1 Decembrie st., Oradea, 410068 (RO)
  • Simona CAVALU University of Oradea, Faculty of Medicine and Pharmacy, 10 Piaţa 1 Decembrie st., Oradea, 410068 (RO)
  • Angela ANTONESCU University of Oradea, Faculty of Medicine and Pharmacy, 10 Piaţa 1 Decembrie st., Oradea, 410068 (RO)
  • Tudor E. FERTIG Victor Babeș National Institute of Pathology, Ultrastructural Pathology and Bioimaging Lab., Bucharest; “Carol Davila” University of Medicine and Pharmacy, 8 Bulevardul Eroii Sanitari, Bucharest, 050474 (RO)
  • Daciana S. MARTA Victor Babeș National Institute of Pathology, Ultrastructural Pathology and Bioimaging Lab., Bucharest; “Titu Maiorescu” University, Faculty of Medicine, 67/A Gheorghe Petraşcu st., sect. 3, Bucharest (RO)



antioxidant capacity, normal dermal fibroblast, phenols, selenium nanoparticles, scratch assay, Viscum album, wound healing


Mistletoe (Viscum album L.), a semi-parasitic medicinal plant, continues to be of interest, due to its phytochemical composition. The leaves of mistletoe contain phenols, which have a variety of biological effects. The main goal was to characterize the mistletoe that parasitized Robinia pseudoacacia L., (called VAR) in terms of phenolic compounds and to assess the wound healing potential in vitro using the scratch method. Furthermore, the antioxidant capacity was evaluated both by spectrophotometric techniques (DPPH, FRAP, TEAC), as well as by the ability of the mistletoe extract to synthesize green selenium nanoparticles. Among the phenolic acids, dihydroxybenzoic acid is in high level (2.86±0.03 mg/g dw), whereas isorhamnetin-glucuronides dominate the flavonol class (0.593±0.03 mg/g dw). The presence of phenolic compounds in the VAR leaves provides antioxidant capacity. The reducing capacity of VAR extract was demonstrated for the first time by the biosynthesis of nanoselenium particles (NSePs) with a regular, spherical shape and a diameter of around 130 nm. The VAR concentrations of 25-200 µg/mL showed no toxic effect on normal human dermal fibroblasts (NHDF), and the concentration of 100 µg/mL exhibited the best percentage of wound surface closure in vitro (94.08%). The results show that mistletoe is a promising plant because of its phytochemical composition and antioxidant capacity, which can modulate the wound repair process in vitro.


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How to Cite

VICAS, S. I., MEMETE, A. R., KLESZKEN, E., MIERE (GROZA), F., GANEA, M., CAVALU, S., ANTONESCU, A., FERTIG, T. E., & MARTA, D. S. (2024). Phytochemical profile, antioxidant capacity and wound healing potential of Viscum album L. growing on Robinia pseudoacacia L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13537.



Research Articles
DOI: 10.15835/nbha52113537