Enhancing crop resilience through thiamine: implications for sustainable agriculture in drought-stressed radish

Authors

  • Rubab IQBAL University of Agriculture Faisalabad, Department of Botany, 3800, Punjab (PK)
  • Muhammad SHAHBAZ University of Agriculture Faisalabad, Department of Botany, 3800, Punjab (PK)
  • Muhammad Z. MANSHA University of Layyah, College of Agriculture, Department of Plant Pathology, Layyah, 31200, Punjab (PK)
  • Kamran IKRAM Khawaja Fareed University of Engineering and Information Technology, Department of Agricultural Engineering, Rahim Yar Khan, 64200 (PK)
  • Imran KHALID The Islamia University of Bahawalpur, Faculty of Agriculture and Environment, Department of Agriculture Extension Education, 63100 (PK)
  • Usman TARIQ Govt. College University Faisalabad, Department of Botany, Layyah Campus, Layyah, 31200, Punjab (PK)
  • Manzer. H. SIDDIQUI King Saud University, College of Science, Department of Botany and Microbiology, Riyadh-11451 (SA)
  • Abdel-Rhman Z. GAAFAR King Saud University, College of Science, Department of Botany and Microbiology, Riyadh-11451 (SA)
  • Mohamed S. HODHOD October University for Modern Sciences & Arts, Faculty of Biotechnology, 6th October City, 12566 (EG)
  • Kamran ASHRAF East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai 200237 (CN)
  • Qamar uz ZAMAN The University of Lahore, Department of Environmental Sciences, Lahore, 54590 (PK)

DOI:

https://doi.org/10.15835/nbha52113472

Keywords:

Antioxidants potential, drought stress, foliar, growth, osmoprotectants, radish

Abstract

During 21st century, abiotic stress has adversely affected the agriculture crop production around the globe. Keeping in view the food requirement under water shortage condition, a study was planned to investigate the effect of thiamine application on radish crop under drought stress conditions on plant. For study purpose, two varieties of locally available radish (‘Early-Milo’ and ‘Laal-Pari’) were grown with normal water application as well as thiamine (100 mg L-1) application while maintaining a stress condition (60% field capacity). Increasing water deficit stress linearly reduced plant growth, yield and biomass in both varieties by reducing water use efficiency, while significantly enhanced these attributes with thiamine application. Thiamine application under drought stress exerted significant impacts on physiological attributes in both varieties, including enhanced osmolytic attribute in drought conditions and improvements in superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), H2O2, and malondialdehyde (MDA) activities in plant leaves. Antioxidant and osmoprotectant upregulation positively linked to radish crop's drought tolerance. Moreover, PCA and heatmap analysis revealed a significant interdependence among various traits and interconnected in determining the crop's capacity to sustain growth under conditions of drought stress. In crux, thiamine application conclusively enhances radish growth, yield, biomass, physio-chemical and osmolytic attributes, ionic composition and enzymatic antioxidant potential. Therefore, it is recommended to consider the application of thiamine in commercial agriculture practices to mitigate the negative effects of drought stress on radish crop production.

References

Abrar MM, Saqib M, Abbas G, Atiq-ur-Rahman M, Mustafa A, Shah SAA, Xu M (2020). Evaluating the contribution of growth, physiological, and ionic components towards salinity and drought stress tolerance in Jatropha curcas. Plants 9(11):1574. https://doi.org/10.3390/plants9111574

Aebi H (1984). Catalase in vitro. Methods in Enzymology 105:121-126. https://doi.org/10.1016/S0076-6879(84)05016-3

Ahmad A, Aslam Z, Hussain S, Javed T, Hussain S, Bashir S, … Hessini K (2022). Soil application of wheat straw vermicompost enhances morpho-physiological attributes and antioxidant defense in wheat under drought stress. Frontiers in Environmental Science 10:894517. https://doi.org/10.3389/fenvs.2022.894517

Ahn IP, Kim S, Lee YH (2005). Vitamin B1 functions as an activator of plant disease resistance. Plant Physiology 138(3):1505-1515. https://doi.org/10.1104/pp.104.058693

Amjad SF, Mansoora N, Yaseen S, Kamal A, Butt B, Matloob H, … Shahbaz M (2021). Combined use of endophytic bacteria and pre-sowing treatment of thiamine mitigates the adverse effects of drought stress in wheat (Triticum aestivum L.) cultivars. Sustainability 13(12):6582. https://doi.org/10.3390/su13126582

Ansari AW, Atri N, Pandey M, Kumar SA, Singh B, Pandey S (2019). Influence of drought stress on morphological, physiological, and biochemical attributes of plants: a review. Biosciences, Biotechnology Research Asia 16(04):697-709. https://doi.org/10.13005/bbra/2785

Atif M, Perveen S (2023). Maize grain nutritional quality amelioration with seed-applied thiamine and indole-3-acetic acid under arsenic toxicity. Environment, Development and Sustainability. https://doi.org/10.1007/s10668-023-03037-y

Ault TR (2020). On the essentials of drought in a changing climate. Science 368(6488):256-260. https://doi.org/10.1126/science.aaz5492

Ayyub CM, Rashid SM, Raza S, Sarwar YM, Khan QRW, Azam M, … Akhtar N (2016). Evaluation of different radish genotypes under different saline regimes. American Journal of Plant Sciences 07(06):894-898. https://doi.org/10.4236/ajps.2016.76084

Baba RM (2019). The effect of foliar application of salicylic acid and thiamine on some physiological and biochemical traits of tuberose (Polianthes tuberosa L.) in soil and soilless cultivation systems. Journal of Science and Technology of Greenhouse Culture 9(4):53-69. https://doi.org/10.29252/ejgcst.9.4.53

Beauchamp C, Fridovich I (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1):276-287. https://doi.org/10.1016/0003-2697(71)90370-8

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2):248-254. https://doi.org/10.1016/0003-2697(76)90527-3

El-Beltagi HS, Maraei RW, Shalaby TA, Aly AA (2022). Metabolites, nutritional quality and antioxidant activity of red radish roots affected by gamma rays. Agronomy 12(8):1916. https://doi.org/10.3390/agronomy12081916

El-Shazoly RM, Metwally AA, Hamada AM (2019). Salicylic acid or thiamin increases tolerance to boron toxicity stress in wheat. Journal of Plant Nutrition 42(7):702-722. https://doi.org/10.1080/01904167.2018.1549670

Gamba M, Asllanaj E, Raguindin PF, Glisic M, Franco OH, Minder B, … Muka T (2021). Nutritional and phytochemical characterization of radish (Raphanus sativus): A systematic review. Trends in Food Science & Technology 113:205-218. https://doi.org/10.1016/j.tifs.2021.04.045

Ghafar MA, Akram NA, Ashraf M, Ashraf MY, Sadiq M (2019). Thiamin-induced variations in oxidative defense processes in white clover (Trifolium repens L.) under water deficit stress. Turkish Journal of Botany 43(1):58-66. https://doi.org/10.3906/bot-1710-34

Giannakoula A, Moustakas M, Mylona P, Papadakis I, Yupsanis T (2008). Aluminum tolerance in maize is correlated with increased levels of mineral nutrients, carbohydrates and proline, and decreased levels of lipid peroxidation and Al accumulation. Journal of Plant Physiology 165(4):385-396. https://doi.org/10.1016/j.jplph.2007.01.014

Goyer A (2010). Thiamine in plants: aspects of its metabolism and functions. Phytochemistry 71(14-15):1615-1624. https://doi.org/10.1016/j.phytochem.2010.06.022

Hamada AM, Fatehi J, Jonsson LMV (2018). Seed treatments with thiamine reduce the performance of generalist and specialist aphids on crop plants. Bulletin of Entomological Research 108(1):84-92. https://doi.org/10.1017/S0007485317000529

Henschel JM, Dantas EFO, Soares VDA, Santos SKD, Santos LWOD, Dias TJ, … Batista DS (2022). Salicylic acid mitigates the effects of mild drought stress on radish. In: Allakhverdiev S (Ed). Functional Plant Biology 49(9):822-831. https://doi.org/10.1071/FP22040

Jabeen M, Akram NA, Ashraf M, Alyemeni MN, Ahmad P (2021). Thiamin stimulates growth and secondary metabolites in turnip (Brassica rapa L.) leaf and root under drought stress. Physiologia Plantarum 172(2):1399-1411. https://doi.org/10.1111/ppl.13215

Jabeen M, Akram NA, Ashraf M, Tyagi A, El-Sheikh MA, Ahmad P (2022). Thiamin stimulates growth, yield quality and key biochemical processes of cauliflower (Brassica oleracea L. var. botrytis) under arid conditions. Plos One 17(5):e0266372. https://doi.org/10.1371/journal.pone.0266372

Jambunathan N (2010). Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants. In: Sunkar R (Ed). Plant Stress Tolerance 639:291-297. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-702-0_18

Kausar A, Zahra N, Zahra H, Hafeez MB, Zafer S, Shahzadi A, … Prasad PV (2023). Alleviation of drought stress through foliar application of thiamine in two varieties of pea (Pisum sativum L.). Plant Signaling & Behavior 18(1):2186045. https://doi.org/10.1080/15592324.2023.2186045

Kaya C, Ashraf M, Sonmez O, Tuna AL, Polat T, Aydemir S (2015). Exogenous application of thiamin promotes growth and antioxidative defense system at initial phases of development in salt-stressed plants of two maize cultivars differing in salinity tolerance. Acta Physiologiae Plantarum 37(1):1741. https://doi.org/10.1007/s11738-014-1741-3

Kaya C, Aslan M, Uğurlar F, Ashraf M (2020). Thiamine-induced nitric oxide improves tolerance to boron toxicity in pepper plants by enhancing antioxidants. Turkish Journal of Agriculture and Forestry 44(4):379-390. https://doi.org/10.3906/tar-1909-40

Lacerda VR, Acevedo AFG, Marques ICDS, Dellabiglia WJ, Ferraz AKL, Basílio LSP, … Broetto F (2022). Silicon as a mitigator of water deficit stress in radish crop. Scientia Horticulturae 291:110600. https://doi.org/10.1016/j.scienta.2021.110600

Lv J, Zong X, Shakeel AA, Wu X, Wu C, Li Y, Wang S (2020). Alteration in morpho-physiological attributes of Leymus chinensis (Trin.) tzvelev by exogenous application of brassinolide under varying levels of drought stress. Chilean Journal of Agricultural Research 80(1):61-71. https://doi.org/10.4067/S0718-58392020000100061

Maehly AC (2006). The assay of catalases and peroxidases. In Glick D (Ed). Methods of Biochemical Analysis. Hoboken, NJ, USA, John Wiley & Sons, Inc. pp 357-424. https://doi.org/10.1002/9780470110171.ch14

Malik MA, Wani AH, Mir SH, Rehman IU, Tahir I, Ahmad P, … Rashid I (2021). Elucidating the role of silicon in drought stress tolerance in plants. Plant Physiology and Biochemistry 165:187-195. https://doi.org/10.1016/j.plaphy.2021.04.021

Manzoor A, Bashir MA, Naveed MS, Cheema KL, Cardarelli M (2021). Role of different abiotic factors in inducing pre-harvest physiological disorders in radish (Raphanus sativus). Plants 10(10):2003. https://doi.org/10.3390/plants10102003

Marcelis LFM, Van HJ (1999). Effect of salinity on growth, water use and nutrient use in radish (Raphanus sativus L.). Plant and Soil 215(1):57-64. https://doi.org/10.1023/A:1004742713538

Martinis J, Gas-Pascual E, Szydlowski N, Crèvecoeur M, Gisler A, Bürkle L, … Fitzpatrick TB (2016). Long-distance transport of thiamine (vitamin B1) is concomitant with that of polyamines. Plant Physiology 171(1):542-553. https://doi.org/10.1104/pp.16.00009

Matamoros V, Casas ME, Mansilla S, Tadić Đ, Cañameras N, Carazo N, … Bayona JM (2022). Occurrence of antibiotics in lettuce (Lactuca sativa L.) and radish (Raphanus sativus L.) following organic soil fertilisation under plot-scale conditions: crop and human health implications. Journal of Hazardous Materials 436:129044. https://doi.org/10.1016/j.jhazmat.2022.129044

Mehrabad PS, Fabriki-Ourang S, Mehrabi AA (2019). Expression of dehydrin and antioxidant genes and enzymatic antioxidant defense under drought stress in wild relatives of wheat. Biotechnology & Biotechnological Equipment 33(1):1063-1073. https://doi.org/10.1080/13102818.2019.1638300

Morais MBD, Camara TR, Ulisses C, Carvalho Filho JLS, Willadino L (2018). Multiple stresses on the oxidative metabolism of sugarcane varieties. Ciência Rural 48(4). https://doi.org/10.1590/0103-8478cr20141487

Mozafar A (1994). Enrichment of some B-vitamins in plants with application of organic fertilizers. Plant and Soil 167(2):305-311. https://doi.org/10.1007/BF00007957

Nagae M, Parniske M, Kawaguchi M, Takeda N (2016). The relationship between thiamine and two symbioses: root nodule symbiosis and arbuscular mycorrhiza. Plant Signaling & Behavior 11(12):e1265723. https://doi.org/10.1080/15592324.2016.1265723

Naheed R, Zahid M, Aqeel M, Maqsood MF, Kanwal H, Khalid N, … Noman A (2022). Mediation of growth and metabolism of pisum sativum in salt stress potentially be credited to thiamine. Journal of Soil Science and Plant Nutrition 22(3):2897-2910. https://doi.org/10.1007/s42729-022-00854-4

Nishio T (2017). Economic and academic importance of radish. In: Nishio T, Kitashiba H (Eds). The Radish Genome. Cham, Springer International Publishing. p 1-10. (Compendium of Plant Genomes). https://doi.org/10.1007/978-3-319-59253-4_1

Omar A, Zayed B, Salam AA, Hafez YM, Abdelaal KA (2020). Folic acid as foliar application can improve growth and yield characters of rice plants under irrigation with drainage water. Fresenius Environmental Bulletin 29(10):9420-9428. https://www.prt-parlar.de/download_feb_2020/

Ors S, Suarez DL (2017). Spinach biomass yield and physiological response to interactive salinity and water stress. Agricultural Water Management 190:31-41. https://doi.org/10.1016/j.agwat.2017.05.003

Pearcy RW, Schulze ED, Zimmermann R (2000). Measurement of transpiration and leaf conductance. In: Pearcy RW, Ehleringer JR, Mooney HA, Rundel PW (Eds). Plant Physiological Ecology. Dordrecht, Springer Netherlands. pp 137-160. https://doi.org/10.1007/978-94-010-9013-1_8

Rady MM, Boriek SHK, Abd ETA, Seif EMA, Ali EF, Hassan FAS, … Abdelkhalik A (2021). Exogenous gibberellic acid or dilute bee honey boosts drought stress tolerance in vicia faba by rebalancing osmoprotectants, antioxidants, nutrients, and phytohormones. Plants 10(4):748. https://doi.org/10.3390/plants10040748

Ramos EB, Ramos SB, Ramos SB, De Figueiredo PAM, Da-Silva VR, Vendruscolo EP, … De LSF (2023). Does exogenous vitamins improve the morphophysiological condition of sugarcane subjected to water deficit?. Sugar Technology 25(1):262–267. https://doi.org/10.1007/s12355-022-01177-5

Rodrigues DQA, Hines C, Brown J, Sahay S, Vijayan J, Stone JM, … Roston RL (2023). The effects of exogenously applied antioxidants on plant growth and resilience. Phytochemistry Reviews 22(2):407-447. https://doi.org/10.1007/s11101-023-09862-3

Rodriguez CM, Avalos J, Bonet ML, Boronat A, Gomez-Gomez L, Hornero-Mendez D, … Zhu C (2018). A global perspective on carotenoids: metabolism, biotechnology, and benefits for nutrition and health. Progress in Lipid Research 70:62-93. https://doi.org/10.1016/j.plipres.2018.04.004

Sadak M, El-Bassiouny H, Mahfouz S, El-Enany M, Elewa T (2022). Use of thiamine, pyridoxine and biostimulant for better yield of wheat plants under water stress: growth, osmoregulations, antioxidantive defense and protein pattern. Egyptian Journal of Chemistry 66(4):407-424. https://doi.org/10.21608/ejchem.2022.160140.6898

Sahay S, Khan E, Gupta M (2019). Nitric oxide and abscisic acid protects against PEG-induced drought stress differentially in brassica genotypes by combining the role of stress modulators, markers and antioxidants. Nitric Oxide 89:81-92. https://doi.org/10.1016/j.niox.2019.05.005

Seifi A, Poustini K, Ahmadi A (2023). The effect of thiamine and pyridoxine on yield and some physiological wheat traits under drought stress conditions. Australian Journal of Crop Science 54(1):11-25. http://dx.doi.org/10.22059/ijfcs.2022.328186.654884

Seleiman MF, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, … Battaglia ML (2021). Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants 10(2):259. https://doi.org/10.3390/plants10020259

Sergiev L, Alexieve V, Karanov E (1997). Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes rendus de l’Academie bulgare des Sciences 5:121-124.

Srivastav AL, Dhyani R, Ranjan M, Madhav S, Sillanpää M (2021). Climate-resilient strategies for sustainable management of water resources and agriculture. Environmental Science and Pollution Research 28 (31):41576–41595. https://doi.org/10.1007/s11356-021-14332-4

Stagnari F, Galieni A, D'Egidio S, Pagnani G, Pisante M (2018). Responses of radish (Raphanus sativus L. ) to drought stress: Radish adapted to drought through avoidance mechanisms. Annals of Applied Biology 172(2):170-186. https://doi.org/10.1111/aab.12409

Steel RGD, Torrie JH, Dicky DA (1997) Principles and procedures of statistics: A biometric approach. Mc Graw Hill Inc, New York.

Sultan K, Perveen S, Parveen A, Atif M, Zafar S (2023). Benzyl amino purine (BAP), moringa leaf extract and ascorbic acid induced drought stress tolerance in pea (Pisum sativum L.). Gesunde Pflanzen. https://doi.org/10.1007/s10343-023-00890-9

Tan ZH, Wu ZX, Hughes AC, Schaefer D, Zeng J, Lan GY, … Yang LY (2017). On the ratio of intercellular to ambient CO2 (c i/c a) derived from ecosystem flux. International Journal of Biometeorology 61(12):2059–2071. https://doi.org/10.1007/s00484-017-1403-4

Tiepo AN, Constantino LV, Madeira TB, Gonçalves LSA, Pimenta JA, Bianchini E, … Stolf-Moreira R (2020). Plant growth-promoting bacteria improve leaf antioxidant metabolism of drought-stressed neotropical trees. Planta 251(4):83. https://doi.org/10.1007/s00425-020-03373-7

Tufail B, Ashraf K, Abbasi A, Ali HM, Sultan K, Munir T, … Uz Zaman Q (2023). Effect of selenium on growth, physio-biochemical and yield traits of lettuce under limited water regimes. Sustainability 15(8):6804. https://doi.org/10.3390/su15086804

Turner NC, Kramer PJ (1980). Adaptation of plants to water and high temperature stress. New York, Wiley. Pp 482.

Tuver GY, Ekinci M, Yildirim E (2022). Morphological, physiological and biochemical responses to combined cadmium and drought stress in radish (Raphanus sativus L.). rendiconti lincei. Scienze Fisiche e Naturali 33(2):419-429. https://doi.org/10.1007/s12210-022-01062-z

Velikova V, Yordanov I, Edreva A (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Science 151(1):59-66. https://doi.org/10.1016/S0168-9452(99)00197-1

Vendruscolo EP, Dantas AV, Rodrigues SG, Pacheco DAP, Bortolheiro F, Battistuzzi MM, … Dantas T (2022). Do exogenous application of thiamine mitigates low soil base saturation effects on bell pepper plants?. Revista De Agricultura Neotropical 9(3):e6803. https://doi.org/10.32404/rean.v9i3.6803

Xiong D, Nadal M (2020). Linking water relations and hydraulics with photosynthesis. The Plant Journal 101(4):800-815. https://doi.org/10.1111/tpj.14595

Zhang W, Wang SC, Li Y (2023). Molecular mechanism of thiamine in mitigating drought stress in Chinese wingnut (Pterocarya stenoptera): Insights from transcriptomics. Ecotoxicology and Environmental Safety 263:115307. https://doi.org/10.1016/j.ecoenv.2023.115307

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Published

2024-02-22

How to Cite

IQBAL, R., SHAHBAZ, M., MANSHA, M. Z., IKRAM, K., KHALID, I., TARIQ, U., SIDDIQUI, M. H., GAAFAR, A.-R. Z., HODHOD, M. S., ASHRAF, K., & uz ZAMAN , Q. (2024). Enhancing crop resilience through thiamine: implications for sustainable agriculture in drought-stressed radish. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13472. https://doi.org/10.15835/nbha52113472

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DOI: 10.15835/nbha52113472

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