A novel approach for reducing water stress on sunflower plants by using medicinal plant extracts rather than artificial growth regulators

Authors

  • Mohamed A. AL ABBOUD Jazan University, College of Science, Department of Biology, Jazan 82817; Jazan University, Center for Environmental Research and Studies (SA)
  • Mohamed A. AMIN Al-Azhar University, Faculty of Science, Department of Botany and Microbiology, Cairo 11884 (EG)
  • Khatib S. ISMAIL Jazan University, College of Science, Department of Biology, Jazan 82817 (SA)
  • Syed K. ALI Jazan University, College of Science, Department of Chemistry, Jazan, PO Box 114 (SA)
  • M. REMESH Jazan University, College of Science, Department of Biology, Jazan 82817 (SA)
  • Abdelatti I. NOWWAR Al-Azhar University, Faculty of Science, Department of Botany and Microbiology, Cairo 11884 (EG)

DOI:

https://doi.org/10.15835/nbha52113464

Keywords:

biostimulants, drought stress, Helianthus annuus, plant extract

Abstract

    One of the most significant physiological stressors that negatively impact plants in numerous vital areas of their growth and metabolism is water stress. This study estimates the effects of natural bio-stimulants (Origanum majorana, Nigella sativa and Curcurma longa) extracts for the first time, on water stress of sunflower plant in comparison with synthetic growth regulators (glycein betaine, proline, indole acetic acid, benzyl adenine and ascorbic acid). Water stress appeared to decrease of lengths, fresh and dry weights of shoot and root. Also caused a significant drop in chlorophylls and carotenoids. Foliar application of these synthetic and natural growth regulators significantly decreased the negative effects of drought stress on all studied morphological parameters and pigment contents of sunflower. HPLC analysis of bio-stimulants appeared six phenolic acids, one phenol and two flavonoids were found in all natural extracts. Gallic, rosmarinic, caffeic, syringic acids and kaempferol are the major phenolic compounds (more than 1000 µg/g) detected in Origanum majorana. While gallic acid, hesperetin and ferulic acid are the major phenolic compounds (more than 1000 µg/g) present in Curcurma longa. Chlorogenic acid, gallic acid, catechin, pyro catechol, coffeic acid and rutin are the dominant phenolic compounds present in Nigella sativa. The Nigella sativa treatment showed the highest decrease in proline levels. This may be due to the high concentration of caffeic acid (2406.97 g/g). The Origanum majorana treatment had the highest levels of chl. a and carotenoid contents, which rose by about 32% and 72%, respectively, compared to the stress-treated plant. The highly decrease in catalase activity, which is considered as most important indicator to water stress, appeared in Origanum majorana treatment. We suggest the use of these natural extracts as an alternative way, which appeared a significant increase in growth and biochemical near to synthetic regulators in the treatment of water stress due to the fact that these extracts contain many important phenolic compounds that have a role in the treatment of water stress.

References

Adeleke BS, Babalola OO (2020). Oilseed crop sunflower (Helianthus annuus) as a source of food: Nutritional and health benefits. Food Science & Nutrition 8(9):4666-4684. https://doi.org/10.1002/fsn3.1783

Aebi H (1984). Catalase in vitro. Academic Press, pp 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3

Anisimov MM, Skriptsova AV, Chaikina EL, Klykov AG (2013). Effect of water extracts of seaweeds on the growth of seedling roots of buckwheat (Fagopyrum esculentum Moench). International Journal of Research and Reviews in Applied Sciences 16(2):282-287.

Annunziata MG, Ciarmiello LF, Woodrow P, Dell’Aversana E, Carillo P (2019). Spatial and temporal profile of glycine betaine accumulation in plants under abiotic stresses. Frontiers in Plant Science 10:230. https://doi.org/10.3389/fpls.2019.00230

Ashraf M, Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59(2):206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006

Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1):205-207. https://doi.org/10.1007/BF00018060

Bonea D, Urechean V (2018). Effect of sweet marjoram (Origanum majorana L.) cogermination and aqueous extracts on maize (Zea mays L.). “Agriculture for Life, Life for Agriculture” Conference Proceedings 1(1):11-16. https://doi.org/10.2478/alife-2018-0002

Bower AM, Real Hernandez LM, Berhow MA, De Mejia EG (2014). Bioactive compounds from culinary herbs inhibit a molecular target for type 2 diabetes management, dipeptidyl peptidase IV. Journal of Agricultural and Food Chemistry 62(26):6147-6158. https://doi.org/10.1021/jf500639f

Castillo FJ, Penel C, Greppin H (1984). Peroxidase release induced by ozone in sedum album leaves. Plant Physiology 74(4):846-851. https://doi.org/10.1104/pp.74.4.846

Çelik SE, Tufan AN, Bekdeşer B, Özyürek M, Güçlü K, Apak R (2017). Identification and determination of phenolics in Lamiaceae species by UPLC-DAD-ESI-MS/MS. Journal of Chromatographic Science 55(3):291-300. https://doi.org/10.1093/chromsci/bmw184

Chun SC, Paramasivan M, Chandrasekaran M (2018). Proline accumulation influenced by osmotic stress in arbuscular mycorrhizal symbiotic plants. Frontiers in Microbiology 9:2525. https://doi.org/10.3389/fmicb.2018.02525

Daniel HD, George C (1972). Peach seed dormancy in relation to endogenous inhibitors and applied growth substances. Journal of the American Society for Horticultural Science 97(5):651-654. https://doi.org/10.21273/JASHS.97.5.651

Duke SO (2010). Allelopathy: Current status of research and future of the discipline: A commentary. Allelo Journal 25:17-30.

Ebrahimi M, Khajehpour M R, Naderi A, Nassiri B M (2014). Physiological responses of sunflower to water stress under different levels of zinc fertilizer. International Journal of Plant Production 8(4).

El Mantawy R, El-Hag D (2018). Effect of skipping irrigation at different growth stages and zinc foliar application on yield and water productivity of sunflower. Journal of Plant Production 9(3):273-279.‏ https://doi.org/10.21608/jpp.2018.35488

Elansary HO, Mahmoud EA (2015). Egyptian herbal tea infusions’ antioxidants and their antiproliferative and cytotoxic activities against cancer cells. Natural Product Research 29(5):474-479. https://doi.org/10.1080/14786419.2014.951354

El-Din G (2015). Alleviation of drought effect on sunflower (Helianthus annus L.) cv Sakha-53 cultivar by foliar spraying with antioxidant. Middle East Journal 4(4):794-801.

Elena B (2014). Elemente de bioinginerie agricolă. Editura Sitech, Craiova, pp 12.

Gebaly S, Ahmed F, Namich A (2013). effect of spraying some organic, amino acids and potassium citrate on alleviation of drought stress in cotton plant. Journal of Plant Production 4(9):1369-1381. https://doi.org/10.21608/jpp.2013.74149

Haghighi M, Saadat S, Abbey L (2020). Effect of exogenous amino acids application on growth and nutritional value of cabbage under drought stress. Scientia Horticulturae 272:109561. https://doi.org/10.1016/j.scienta.2020.109561

Horoszkiewicz J, Jajor E, Danielewicz J, Korbas M, Schimmelpfennig L, Mikos-Szymańska M, Klimczyk M, Bocianowski, J (2023). The assessment of an effect of natural origin products on the initial growth and development of maize under drought stress and the occurrence of selected pathogens. Agriculture 13(4):815.‏ https://doi.org/10.3390/agriculture13040815

Hussain M, Farooq S, Hasan W, Ul-Allah S, Tanveer M, Farooq M, Nawaz A (2018). Drought stress in sunflower: Physiological effects and its management through breeding and agronomic alternatives. Agricultural Water Management 201(September 2017):152-166. https://doi.org/10.1016/j.agwat.2018.01.028

Ikechukwu OA (2014). Crude Extracts of Senna alata (L.) Roxb. mimics plant growth hormones in promotion of vegetative and reproductive growth in Celosia argentea L. American Journal of Plant Sciences 5:1918-1925. https://doi.org/10.4236/ajps.2014.513205

Im Kim J, Baek D, Park HC, Chun HJ, Oh DH, Lee MK, Chung WS (2013). Overexpression of Arabidopsis YUCCA6 in potato results in high-auxin developmental phenotypes and enhanced resistance to water deficit. Molecular Plant 6(2):337-349.

Kapoor D, Bhardwaj S, Landi M, Sharma A, Ramakrishnan M, Sharma A (2020). The impact of drought in plant metabolism: How to exploit tolerance mechanisms to increase crop production. Applied Sciences 10(16):5692.‏ https://doi.org/10.3390/app10165692

Khazaei Z, Estaji A (2020). Effect of foliar application of ascorbic acid on sweet pepper (Capsicum annuum) plants under drought stress. Acta Physiologiae Plantarum 42:1-12. https://doi.org/10.1007/s11738-020-03106-z

Khazaei Z, Esmaielpour B, Estaji A (2020). Ameliorative effects of ascorbic acid on tolerance to drought stress on pepper (Capsicum annuum L) plants. Physiology and Molecular Biology of Plants 26:1649-1662. https://doi.org/10.1007/s12298-020-00846-7

Kong CH, Xuan TD, Khanh TD, Tran HD, Trung NT (2019). Allelochemicals and signaling chemicals in plants. Molecules 24(15):2737. https://doi.org/10.3390/molecules24152737

Lalarukh I, Al-Dhumri SA, Al-Ani L KT, Hussain R, Al Mutairi KA, Mansoora N, ... Galal TM (2022). A combined use of rhizobacteria and moringa leaf extract mitigates the adverse effects of drought stress in wheat (Triticum aestivum L.). Frontiers in Microbiology 13:813415.‏ https://doi.org/10.3389/fmicb.2022.813415

Lichtentahler (1989). The Science of Photobiology. In: Smith KC (Ed). The Science of Photobiology. Boston, MA: Springer US. https://doi.org/10.1007/978-1-4615-8061-4

Lowry OH, Rosebrough, NJ, Farr AL, Randall RJ (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265-275. https://doi.org/10.1016/S0021-9258(19)52451-6

Maharshi A, Rashid MM, Teli B, Yadav SK, Singh DP, Sarma BK (2021). Salt stress alters pathogenic behaviour of Fusarium oxysporum f. sp. ciceris and contributes to severity in chickpea wilt incidence. Physiological and Molecular Plant Pathology 113:101602. https://doi.org/10.1016/j.pmpp.2021.101602

Mangena P (2022). Evolving role of synthetic cytokinin 6-benzyl adenine for drought stress tolerance in soybean (Glycine max L. Merr.). Frontiers in Sustainable Food Systems 6:992581. https://doi.org/10.3389/fsufs.2022.992581

Marklund S, Marklund G (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47(3):469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Matta A, Diamoned C (1963). Symptoms of Fusarium wilt in relation to quantity of fungus and enzyme activity in tomato stems. Phytopathology 53(5):574-587.

Mechraoui O, Ladjel S, Nedjimi MS, Belfar ML, Moussaoui Y (2018). Determination of polyphenols content, antioxidant and antibacterial activity of Nigella sativa L. seed phenolic extracts. Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry 19(4):411.

Mehmood H, Abbasi GH, Jamil M, Malik Z, Ali M, Iqbal R (2021). Assessing the potential of exogenous caffeic acid application in boosting wheat (Triticum aestivum L.) crop productivity under salt stress. Plos One 16(11):e0259222. https://doi.org/10.1371/journal.pone.0259222

Mohammadi A, Habibi D, Rohami M, Mafakheri S (2011). Effect of drought stress on antioxidant enzymes activity of some chickpea cultivars. American-Eurasian Journal of Agriculture and Environmental Sciences 11(6):782-785.

Muhammad N, Hakim UMQ, Quraishi UM, Chaudhary HJ, Munis MFH (2016). Indole-3-acetic acid induces biochemical and physiological changes in wheat under drought stress conditions. Philippine Agriculture Science 99(1):19-24.‏

Mukherjee SP, Choudhuri MA (1983). Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia Plantarum 58(2):166-170. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x

Nawaz M, Wang Z (2020). Abscisic acid and glycine betaine mediated tolerance mechanisms under drought stress and recovery in Axonopus compressus: A new insight. Scientific Reports 10(1):1-10. https://doi.org/10.1038/s41598-020-63447-0

Osakabe Y, Osakabe K, Shinozaki K, Tran LSP (2014). Response of plants to water stress. Frontiers in Plant Science 5:86. https://doi.org/10.3389/fpls.2014.00086

Rasheed R, Yasmeen H, Hussain I, Iqbal M, Ashraf M A, Parveen A (2020). Exogenously applied 5-aminolevulinic acid modulates growth, secondary metabolism and oxidative defense in sunflower under water deficit stress. Physiology and Molecular Biology of Plants 26:489-499. https://doi.org/10.1007/s12298-019-00756-3

SeleimanM F, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, Battaglia ML (2021). Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants 10(2):1-25. https://doi.org/10.3390/plants10020259

Shi H, Chen L, Ye T, Liu X, Ding K, Chan Z (2014). Modulation of auxin content in Arabidopsis confers improved drought stress resistance. Plant Physiology and Biochemistry 82:209-217. https://doi.org/10.1016/j.plaphy.2014.06.008

Skowron E, Magdalena T (2021). Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley. Biologia 76(2):453-468. https://doi.org/10.2478/s11756-020-00644-2

Snedecor GW, Cochran WG (1980). Statistical Methods. 7" ed. lowa State Univ. Press, lowa, USA, pp 325-330. https://doi.org/10.1038/clpt.1985.194

Todorova D, Katerova Z, Shopova E, Brankova L, Sergiev I, Jankauskienė J, Jurkonienė S (2022). The physiological responses of wheat and maize seedlings grown under water deficit are modulated by pre-application of auxin-type plant growth regulators. Plants 11(23). https://doi.org/10.3390/plants11233251

Umbreit WW, Burris RH, Stauffer JF (1957). Manometric techniques. A manual describing methods applicable to the study of tissue metabolism. Manometric techniques. A manual describing methods applicable to the study of tissue metabolism. Burgess Publishing Co.

Vernon LP, Seely GR (2014). The chlorophylls. Academic press.

Yadav B, Jogawa, A, Gnanasekaran P, Kumari P, Lakra N, Lal SK, Narayan OP (2021). An overview of recent advancement in phytohormones-mediated stress management and drought tolerance in crop plants. Plant Gene 25:100264. https://doi.org/10.1016/j.plgene.2020.100264

Zhang Y, Li Y, Hassan MJ, Li Z, Peng Y (2020). Indole-3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes. BMC Plant Biology 20(1):1-12. https://doi.org/10.1186/s12870-020-02354-y

Downloads

Published

2024-03-12

How to Cite

AL ABBOUD, M. A., AMIN, M. A., ISMAIL, K. S., ALI, S. K., REMESH, M., & NOWWAR, A. I. (2024). A novel approach for reducing water stress on sunflower plants by using medicinal plant extracts rather than artificial growth regulators. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13464. https://doi.org/10.15835/nbha52113464

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha52113464