Understanding the influence of applying plant extracts and microorganism culture filtrates against barley leaf rust disease

Authors

  • B.R. MOHDLY Barley Dis. Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza (EG)
  • Fatmah AHMED SAFHI Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, P.O. Box 84428, Riyadh 11671 (SA)
  • M.A. ABOU-ZEID Wheat Dis. Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza (EG)
  • A.A. ABDEL-FATTAH Barley Dis. Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza (EG)
  • Ameina S. ALMOSHADAK King Abdulaziz University, Faculty of Science, Department of Biological Sciences, Jeddah 21589 (SA)
  • Enas A. ALMANZALAWI King Abdulaziz University, Faculty of Science, Department of Biological Sciences, Jeddah 21589 (SA)
  • Tahani M. ALQAHTANI King Abdulaziz University, Faculty of Science, Department of Biological Sciences, Jeddah 21589 (SA)
  • Diaa ABD EL MONEIM Arish University, Faculty of Environmental Agricultural Sciences, Department of Plant Production (Genetic Branch), El-Arish 45511 (EG)
  • Rana A.M. ELESSAWY Barley Dis. Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza (EG)

DOI:

https://doi.org/10.15835/nbha52113450

Keywords:

Hordeum vulgare, leaf rust, Puccinia hordei, induced resistance, plant extracts, SCoT, polyphenol oxidase (PPO), peroxidase (POX), total phenolics

Abstract

Rust fungi are devastating plant pathogens, and several Puccinia species have exerting substantial financial impacts on global barley cultivation. Fungicides are used on a large scale as an effective method for combating phytopathogenic fungi. The negative environmental impacts of fungicides are steadily escalating on a daily basis. Consequently, researchers are currently exploring alternative approaches to mitigate the use of fungicides, such as the utilization of plant extracts. This method has proven effective due to its incorporation of natural antifungal substances. Among the nine natural elicitors that were tested, the application of plant extracts on barley seedlings resulted in an increase in the incubation and latent periods of Puccinia hordei. These periods are integral components of partial and induced resistance, effectively mitigating the incidence of barley leaf rust disease by over 70% on mature plants. Similarly, the biochemical analyses demonstrated a notable augmentation in all the tested treatments’ overall phenolics and oxidative enzyme activities (peroxidase and polyphenol oxidase). Random amplified polymorphic DNA (SCoT) test serves as a viable approach for assessing the impact of plant extracts and microorganisms on barley plants. The results obtained from this study indicate that the detection of DNA polymorphism through SCoT analysis holds a significant potential powerful tool to evaluate genetic changes compared with untreated plants although some of them tested displayed high similarities at the morphological reaction.

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2024-02-22

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MOHDLY, B., AHMED SAFHI, F., ABOU-ZEID, M., ABDEL-FATTAH, A., ALMOSHADAK, A. S., ALMANZALAWI, E. A., ALQAHTANI, T. M., ABD EL MONEIM, D., & ELESSAWY, R. A. (2024). Understanding the influence of applying plant extracts and microorganism culture filtrates against barley leaf rust disease. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13450. https://doi.org/10.15835/nbha52113450

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DOI: 10.15835/nbha52113450

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