Biochemical, physiological, and molecular responses of diverse olive cultivars to different irrigation regimes


  • Bassam F. ALOWAIESH Jouf University, College of Science, Biology Department, Sakaka, Aljouf 72341 (SA)
  • Ahmed B. EL-MANSY Arish University, Faculty of Environmental and Agricultural Sciences, Department of Plant Production, Vegetables Branch, El-Arish 45511 (EG)
  • Dina A. EL-ALAKMY Arish University, Faculty of Environmental Agricultural Sciences, Department of Plant Production, Fruit Branch, El-Arish 45511 (EG)
  • Esraa A. REHEMA Arish University, Faculty of Environmental and Agricultural Sciences, Department of Soil and Water, El-Arish 45511 (EG)
  • Diaa ABD EL-MONEIM Arish University, Faculty of Environmental Agricultural Sciences, Department of Plant Production, Genetic Branch, El-Arish 45511 (EG)



catalase, chemical constituents, DHN, DREB, drought tolerance, olive (Olea europaea L.), qPCR, vegetative characteristics


Olive is an essential industrial crop in the Mediterranean region with valuable economic and agricultural concerns. Despite its drought resistance, its productivity is restricted by extreme drought stress. Olive cultivars display considerable variation in response mechanisms to drought stress. Accordingly, the impact of mild and extreme water deprivation over two seasons compared to full irrigation requirements on growth and physiological characteristics of three diverse olive cultivars. Three olive cultivars, ‘Manzanillo’, ‘Eggizi-Shami’, and ‘Tofaahi’, were evaluated under three irrigation regimes 100% ETc, 75% ETc, and 50% ETc. Characteristics of shoot and root, as well as physio-chemical constituents, were determined. Besides, the gene expression of dehydration-responsive element binding (DREB), dehydrin (DHN), and catalase (CAT) genes in olive cultivars were explored under different irrigation regimes. The results indicated a substantial impact of irrigation level on all studied parameters. The mild and extreme drought stress treatments caused a gradual reduction in nitrogen, phosphorus, and potassium content, relative water content, root and shoot length, root and leaf numbers, branch count, and leaf area across both seasons. Conversely, proline content was considerably increased under drought treatments compared to well-watered conditions. Similarly, the assessed cultivars exhibited significant variation in all studied parameters, with ‘Eggizi-Shami’ demonstrating superiority. Under mild and extreme drought stress conditions, the cultivar ‘Eggizi-Shami’ displayed the highest proline content and most growth characteristics. Besides, the real-time quantitative PCR (RT-qPCR) analysis displayed significant alterations in gene expression of the tested three genes related to drought response (DHN, DREB, and CAT). The RT-qPCR analysis revealed that under drought stress conditions (75% and 50% ETc), ‘Eggizi-Shami’ exhibited higher expression compared to the other two cultivars (‘Tofaahi’ and ‘Manzanillo’). Combining the results of morphological and physiological parameters with gene expression analysis of drought-related genes can offer highly validated information about drought-tolerant olive cultivars. This integrated approach serves as an innovative methodology to identify and confirm genes involved in abiotic stress.


Abaza GSM, Awaad HA, Attia ZM, Abdel-lateif KS, Gomaa MA, Abaza SMSM, Mansour E (2020). Inducing potential mutants in bread wheat using different doses of certain physical and chemical mutagens. Plant Breeding and Biotechnology 8(3):252-264.

Abd El-Moneim DA, Mohamed IN; Belal AH, Atta ME (2008) Screening bread wheat genotypes for drought tolerance 2- genetic parameters, associations and path coefficient analysis. Arab Universities Journal of Agricultural Sciences 16(2):351-358.

Abd El Moneim DA, Mohamed IN, Belal AH, Atta ME (2010). Screening bread wheat genotypes for drought tolerance: Germination, radical growth and mean performance of yield and its components. In: Economics of Drought and Drought Preparedness in A Climate Change Context. Zaragoza: CIHEAM / FAO / ICARDA /GDAR/CEIGRAM/ MARM (Options Méditerranéennes: Série A. Séminaires Méditerranéens; n. 95) pp 301-305

Abd El-Moneim D (2020) Characterization of ISSR and SCoT markers and TaWRKY gene expression in some Egyptian wheat genotypes under drought stress. Journal of Plant Production Sciences 8:31-46.

Abd El-Moneim D, Alqahtani MM, Abdein MA, Germoush MO (2020). Drought and salinity stress response in wheat: physiological and TaNAC gene expression analysis in contrasting Egyptian wheat genotypes. Journal of Plant Biotechnology 47:1-14.

Abd El-Moneim D, ELsarag EIS, Aloufi S, El-Azraq AM, ALshamrani SM, Safhi FAA, Ibrahim AA (2021). Quinoa (Chenopodium quinoa Willd.): genetic diversity according to ISSR and SCoT markers, relative gene expression, and morpho-physiological variation under salinity stress. Plants 10:2802.

Agarwal PK, Agarwal P, Reddy MK, Sopory SK (2006). Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Reports 25:1263-1274.

Agüero Alcaras LM, Rousseaux MC, Searles PS (2021). Yield and water productivity responses of olive trees (cv. Manzanilla) to post-harvest deficit irrigation in a non-Mediterranean climate. Agricultural Water Management 245:106562.

Ali MMAEH, Mansour E, Awaad HA (2021). Drought tolerance in some field crops: State of the art review. In: Awaad H, Abu-hashim M, Negm A (Eds). Mitigating Environmental Stresses for Agricultural Sustainability in Egypt. Springer Water. Springer, Cham pp 17-62.

Alowaiesh FB (2007) Flowering, pollination and fruiting of some introduced olive cultivars in Al-Jouf region. Master Thesis.

Allan R, Pereira L, Smith M (1998). Crop evapotranspiration-Guidelines for computing crop water requirements-FAO Irrigation and drainage paper 56. FAO Rome 300(9):D05109.

Al Rashed M, Sefelnasr A, Sherif M, Murad A, Alshamsi D, Aliewi A, Ebraheem AA (2023). Novel concept for water scarcity quantification considering nonconventional and virtual water resources in arid countries: Application in Gulf Cooperation Council countries. Science of the Total Environment 882:163473.

Bacelar EA, Correia CM, Moutinho-Pereira JM, Gonçalves BC, Lopes JI, Torres-Pereira JM (2004). Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions. Tree Physiology 24:233-239.

Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207.

Blake G, Hartge KH (1986). Bulk density. Methods of soil analysis: Part 1 Physical and mineralogical methods. Wiley Online Library, pp 363-375.

Bosabalidis AM, Kofidis G (2002). Comparative effects of drought stress on leaf anatomy of two olive cultivars. Plant Science 163:375-379.

Boudiar R, Alshallash KS, Alharbi K, Okasha SA, Fenni M, Mekhlouf A, Fortas B, Hamsi K, Nadjem K, Belagrouz A (2022). Influence of tillage and cropping systems on soil properties and crop performance under semi-arid conditions. Sustainability 14(18):11651.

Boussadia O, Mariem FB, Mechri B, Boussetta W, Braham M, Hadj SBE (2008). Response to drought of two olive tree cultivars (cv Koroneki and Meski). Scientia Horticulturae 116:388-393.

Brito C, Dinis LT, Moutinho-Pereira J, Correia CM (2019). Drought stress effects and olive tree acclimation under a changing climate. Plants 8(7):232.

Brown J, Lilleland O (1946). Rapid determination of potassium and sodium in plant materials and soil extracts by flame photometry. American Society for Horticultural Science 701:341-346.

Calvo-Polanco M, Ruiz-Lozano JM, Azcón R, Molina S, Beuzon CR, García JL, Cantos M, Aroca R (2019). Phenotypic and molecular traits determine the tolerance of olive trees to drought stress. Plant Physiology and Biochemistry 139:521-527.

Cherbiy-Hoffmann SU, Searles PS, Hall AJ, Rousseaux MC (2012). Influence of light environment on yield determinants and components in large olive hedgerows following mechanical pruning in the subtropics of the Southern Hemisphere. Scientia Horticulturae 137:36-42.

Desoky ESM, Alharbi K, Rady MM, Elnahal AS, Selem E, Arnaout SM, Mansour E (2023). Physiological, biochemical, anatomical, and agronomic responses of sesame to exogenously applied polyamines under different irrigation regimes. Agronomy 13(3):875.

ElSayed AI, Mohamed AH, Rafudeen MS, Omar AA, Awad MF, Mansour E (2022). Polyamines mitigate the destructive impacts of salinity stress by enhancing photosynthetic capacity, antioxidant defense system and upregulation of calvin cycle-related genes in rapeseed (Brassica napus L.). Saudi Journal of Biological Sciences 29(5):3675-3686.

Ennajeh M, Tounekti T, Vadel AM, Khemira H, Cochard H (2008). Water relations and drought-induced embolism in olive (Olea europaea) varieties ‘Meski’ and ‘Chemlali’ during severe drought. Tree Physiology 28:971-976.

Ennajeh M, Vadel A, Khemira H, Ben Mimoun M, Hellali R (2006). Defense mechanisms against water deficit in two olive (Olea europaea L.) cultivars ‘Meski’and ‘Chemlali’. The Journal of Horticultural Science and Biotechnology 81:99-104.

Ennajeh M, Vadel AM, Cochard H and Khemira H (2010). Comparative impacts of water stress on the leaf anatomy of a drought-resistant and drought-sensitive olive cultivar. The Journal of Horticultural Science and Biotechnology 85:289-294.

FAOSTAT (2023). Food and Agriculture Organization of the United Nations. Statistical Database. Retrieved August 10th 2023 from:

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: Effects, mechanisms and management. In: Lichtfouse E, Navarrete M, Debaeke P, Véronique S, Alberola C (Eds). Sustainable Agriculture. Springer, Dordrecht pp 153-188.

Ghazy MI, Hamad HS, Gewaily EE, Bleih EM, Arafat EF, El-Kallawy WH, ... Abd El Moneim D (2023) Impacts of kinetin implementation on leaves, floral and root-related traits during seed production in hybrid rice under water deficiency. BMC Plant Biology 23:398.

Gracia M, Mansour E, Casas A, Lasa J, Medina B, Cano JLM, Moralejo M, López A, Fuster PL, Escribano J (2012). Progress in the Spanish national barley breeding program. Spanish Journal of Agricultural Research 10(3):741-751.

Guerfel M, Baccouri O, Boujnah D, Chaïbi W, Zarrouk M (2009). Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Scientia Horticulturae 119:257-263.

Gowayed SM, Abd El-Moneim D (2021). Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress. PLoS ONE 16(3):e0248890.

Hanin M, Brini F, Ebel C, Toda Y, Takeda S, Masmoudi K (2011). Plant dehydrins and stress tolerance: versatile proteins for complex mechanisms. Plant Signaling and Behavior 6:1503-1509.

Hosseini S, Soleimani A, Taheri M, Tavakoli A (2013). Drought tolerance indices in some olive cultivars (Olea europaea L.). Seed and Plant Improvement Journal 29:211-226.

Kamara MM, Ibrahim KM, Mansour E, Kheir AM, Germoush MO, Abd El-Moneim D, Motawei MI, Alhusays AY, Farid MA, Rehan M (2021). Combining ability and gene action controlling grain yield and its related traits in bread wheat under heat stress and normal conditions. Agronomy 11(8):1450.

Karan R, DeLeon T, Biradar H, Subudhi PK (2012). Salt Stress-induced variation in DNA methylation pattern and its influence on gene expression in contrasting rice genotypes. PLoS One 7:e40203.

Karimi S, Hojati S, Eshghi S, Nazary MR, Jandoust S (2012). Magnetic exposure improves tolerance of fig ‘Sabz’ explants to drought stress induced in vitro. Scientia Horticulturae 137:95-99.

Karimi S, Rahemi M, Rostami AA, Sedaghat S (2018). Drought effects on growth, water content and osmoprotectants in four olive cultivars with different drought tolerance. International Journal of Fruit Science 18:254-267.

Khan MT, Ahmed S, Sardar R, Shareef M, Abbasi A, Mohiuddin M, Ercisli S, Fiaz S, Marc RA, Attia K (2022). Impression of foliar-applied folic acid on coriander (Coriandrum sativum L.) to regulate aerial growth, biochemical activity, and essential oil profiling under drought stress. Frontiers in Plant Science 13:1005710.

Kidokoro S, Watanabe K, Ohori T, Moriwaki T, Maruyama K, Mizoi J, Myint Phyu Sin Htwe N, Fujita Y, Sekita S, Shinozaki K (2015). Soybean DREB 1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression. The Plant Journal 81(3):505-518.

Kim HB, Kwon M, Ryu H, Fujioka S, Takatsuto S, Yoshida S, An CS, Lee I, Hwang I, Choe S (2006). The regulation of DWARF4 Expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in Arabidopsis. Plant Physiology 140:548-557.

Klute A (1986). Methods of Soil Analysis: Part 1. Physical and Mineralogical Methods. Soil Science Society of America, American Society of Agronomy, Madison, WI, USA.

Liu C, Liu Y, Guo K, Fan D, Li G, Zheng Y, Yu L, Yang R (2011). Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. Environmental and Experimental Botany 71:174-183.

Livak KJ, Schmittgen TD (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods 25:402-408.

Mahmud S, Kamruzzaman M, Bhattacharyya S, Alharbi K, Abd El Moneim D, Mostofa MG (2023). Acetic acid positively modulates proline metabolism for mitigating PEG-mediated drought stress in maize and Arabidopsis. Frontiers in Plant Science 14:1167238.

Mansoor S, Ali A, Kour N, Bornhorst J, AlHarbi K, Rinklebe J, ... Chung YS (2023). Heavy metal induced oxidative stress mitigation and ROS scavenging in plants. Plants 12(16):3003.

Mansour E, El-Sobky ESE, Abdul-Hamid MI, Abdallah E, Zedan AM, Serag AM, Silvar C, El-Hendawy S, Desoky EM (2023). Enhancing Drought tolerance and water productivity of diverse maize hybrids (Zea mays) using exogenously applied biostimulants under varying irrigation levels. Agronomy 13(5):1320.

Mansour E, Moustafa ES, El-Naggar NZ, Abdelsalam A, Igartua E (2018). Grain yield stability of high-yielding barley genotypes under Egyptian conditions for enhancing resilience to climate change. Crop and Pasture Science 69(7):681-690.

Mardinata Z, Edy Sabli T, Ulpah S (2021). Biochemical responses and leaf gas exchange of fig (Ficus carica L.) to water stress, short-term elevated CO2 levels and brassinolide application. Horticulturae 7(4):73.

Mesfer ALshamrani S, Safhi FA, Alshaya DS, Ibrahim AA, Mansour H and Abd El Moneim D (2022) Genetic diversity using biochemical, physiological, karyological and molecular markers of Sesamum indicum L. Frontiers in Genetics 13:1035977.

Mirouze M, Paszkowski J (2011). Epigenetic contribution to stress adaptation in plants. Current Opinion in Plant Biology 14:267-274.

Mohammed BK, Noori IM (2008). Effect of Irrigation levels on the growth and yield of olive trees (Olea europaea L. cv. Ashrasie). Journal of Kirkuk University – Scientific Studies 3:169-184.

Morsi NA, Hashem OS, El-Hady MAA, Abd-Elkrem YM, El-temsah ME, Galal EG, Gad KI, Boudiar R, Silvar C, El-Hendawy S (2023). Assessing drought tolerance of newly developed tissue-cultured canola genotypes under varying irrigation regimes. Agronomy 13(3):836.

Nonis A, Vezzaro A, Ruperti B (2012). Evaluation of RNA extraction methods and identification of putative reference genes for real-time quantitative polymerase chain reaction expression studies on olive (Olea europaea L.) fruits. Journal of Agricultural and Food Chemistry 60:6855-6865.

Novamsky I, Eck RV, Schouwenburg CV, Walinga I (1974). Total nitrogen determination in plant material by means of the indophenol-blue method. Netherlands Journal of Agricultural Science 22:3-5.

Piper C (1950). Soil and plant analysis. Interscience Pub. Inc., New York, pp 212.

Ponce-Molina LJ, María Casas A, Pilar Gracia M, Silvar C, Mansour E, Thomas WB, Schweizer G, Herz M, Igartua E (2012). Quantitative trait loci and candidate loci for heading date in a large population of a wide barley cross. Crop Science 52(6):2469-2480.

Rahemi M, Karimi S, Sedaghat S, Ali Rostami A (2017). Physiological responses of olive cultivars to salinity stress. Advances in Horticultural Science 31:53-59.

Ray DL, Johnson JC (2014). Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real-time RT-PCR. BMC Research Notes 7:304.

Rehman A, Weng J, Li P, Yu J, Rahman Su, Khalid M, Shah IH, Gulzar S, Chang L, Niu Q (2023). Differential response of two contrasting melon (Cucumis melo L.) genotypes to drought stress. Journal of Plant Biology 1-16.

Richards LA (1954). Diagnosis and improvement of saline and alkali soils. Soil Science 78:154.

Riyazuddin R, Nisha N, Singh K, Verma R, Gupta R (2022). Involvement of dehydrin proteins in mitigating the negative effects of drought stress in plants. Plant Cell Reports 41(3):519-533.

Safhi FA, ALshamrani SM, Jalal AS, El-Moneim DA, Alyamani AA, Ibrahim AA (2022). Genetic characterization of some Saudi Arabia’s accessions from commiphora gileadensis using physio-biochemical parameters, molecular markers, DNA barcoding analysis and relative gene expression. Genes 13(11):2099.

Sala F, Arsene GG, Iordănescu O, Boldea M (2015). Leaf area constant model in optimizing foliar area measurement in plants: A case study in apple tree. Scientia Horticulturae 193:218-224.

Selote DS, Khanna-Chopra R (2004). Drought-induced spikelet sterility is associated with an inefficient antioxidant defence in rice panicles. Physiologia Plantarum 121:462-471.

Shaheen MA, Hegazi AA, Hmmam IS (2011). Effect of water stress on vegetative characteristics and leaves chemical constituents of some transplants olive cultivars. American-Eurasian Journal of Agricultural and Environmental Sciences 11:663-670.

Sonmez S, Buyuktas D, Okturen Asri F, Citak S (2008). Assessment of different soil to water ratios (1:1, 1:2.5, 1:5) in soil salinity studies. Geoderma 144:361-369.

Temminghoff EJM, Houba VJG (2004). Plant Analysis Procedures. Springer Netherlands, Dordrecht.

Tian C, Jiang Q, Wang F, Wang G-L, Xu ZS, Xiong AS (2015). Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves. PLoS ONE 10(2):e0117569.

Tiwari P, Indoliya Y, Singh PK, Singh PC, Chauhan PS, Pande V, Chakrabarty D (2019). Role of dehydrin-FK506-binding protein complex in enhancing drought tolerance through the ABA-mediated signaling pathway. Environmental and Experimental Botany 158:136-149.

Trabelsi L, Gargouri K, Hassena AB, Mbadra C, Ghrab M, Ncube B, Van Staden J, Gargouri R (2019). Impact of drought and salinity on olive water status and physiological performance in an arid climate. Agricultural Water Management 213:749-759.

Vitagliano C, L Sebastiani (2002). Physiological and biochemical remarks on environmental stress in olive (Olea europaea L.). Acta Horticulturae 586:435-440.

Zhou M, Hassan MJ, Peng Y, Liu L, Liu W, Zhang Y, Li Z (2021). γ-aminobutyric acid (GABA) priming improves seed germination and seedling stress tolerance associated with enhanced antioxidant metabolism, DREB expression, and dehydrin accumulation in white clover under water stress. Frontiers in Plant Science 12:776939.



How to Cite

ALOWAIESH, B. F., EL-MANSY, A. B., EL-ALAKMY, D. A., REHEMA, E. A., & ABD EL-MONEIM, D. (2023). Biochemical, physiological, and molecular responses of diverse olive cultivars to different irrigation regimes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13395.



Research Articles
DOI: 10.15835/nbha51413395

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