Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

Authors

  • Budi WINARTO Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java (ID)
  • Suskandari KARTIKANINGRUM Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java (ID)
  • Fitri RACHMAWATI Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java (ID)
  • Dewi PRAMANIK Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java (ID)
  • Herni SHINTIAVIRA Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16915, West Java (ID)
  • Mega WEGADARA Ornamental Plant Instrument Standard Assessment Institute, Agriculture Instrument Standard Agency, Agriculture Ministry. Jl. Raya Ciherang, Pacet, Cianjur 43253, West Java (ID)

DOI:

https://doi.org/10.15835/nbha52113324

Keywords:

adventive shoots, filament, in vitro, Lilium sp, ovule

Abstract

Lilies are important cut and pot flowers locally and globally; however, qualified planting materials from in vitro culture are still limited. The culture generally uses scales and leaves, while filaments and ovules are rarely utilized. Filaments and ovules of EXO CF/E, RDF CG/E, and DZ CG/E genotypes of Lilium sp. were explored for their potential to establish in vitro propagation protocol using different Chu N6 medium and their modifications. The 9.9 shoots/explant from 1.5 regenerative explants/replication of RDF CG/E filaments was proven on Chu N6 medium containing 6.79 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.27 µM thidiazuron (TDZ), and 90 g L-1 sucrose; 3.3 regenerative explants/replication and 8.4 shoots/explant of EXO CF/E ovules were determined on Chu N6 medium with 2.26 µM 2,4-D, 6.81 µM TDZ and 90 g L-1 sucrose; and slightly improved on Chu N6 macro elements, full vitamins of MS basal medium with 3.39 µM 2,4-D, 3.41 µM TDZ, 150 ml L-1 coconut water (CW) and 60 g L-1 sucrose. The shoots were proliferated on the established media. The shoots of EXO CF/E, RDF CG/E and DZ CG/E genotypes produced varied leaves/shoot, leaf length, leaf width, roots/shoot, root length and bulb diameter on Chu N6 medium hormone free with 1.5% AC, followed by hardening them in a glass house for one month. The hardened-plantlets were acclimatized in plastic pots containing charcoal husk and bamboo compost (1:1) with 39.8-63.6% survivability. The protocol has the potential to be applied to other genotypes or lilies by specific improvements at the acclimatization stage.

References

Askari N, Visser RGF, Jan De Klerk G (2018). Growth of lily bublets in vitro, a review. International Journal of Horticultural Science and Technology 5(2):133-143. https://doi.org/10.22059/ijhst.2018.268870.263

Bakhshaie M, Babalar M, Mirmasoumi M, Khalighi A (2010). Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species. Plant Cell, Tissue and Organ Culture 102:229-235. https://doi.org/10.1007/s11240-010-9726-4

Bakhshaie M, Khosravi S, Azadi P, Bagheri H, van Tuyl JM (2016). Biotechnological advances in Lilium. Plant Cell Reports 35:1799-1826. https://doi.org/10.1007/s00299-016-2017-8

Deswiniyanti NW, Lestari NKD (2020). In vitro propagation of Lilium longiflorum bulbs using NAA and BAP plant growth regulator treatment. KnE Life Sciences 5(2):32-45. https://doi.org/10.18502/kls.v5i2.6437

Deswiniyanti NW, Lestari NKD, Astarini IA, Hardini Y (2020). In vitro propagation of lily (Lilium longiflorum Thunb.) with growth regulators BAP and NAA. Journal Hortikultura Indonesia 11(2):131-139. https://doi.org/10.29244/jhi.11.2.131-139

El Sawy AM, Aly MM, Mohamed AAA (2021). Profitability of Lilium flowers production as influenced by light and ascorbic acid in green house. African Journal of Agriculture and Food Science 4(1):22-35.

El-Naggar H, Osman A, Sewedan E (2012). In vitro propagation and organogenesis of Lilium ‘Prato’. African Journal of Biotechnology 11(82):14771-14776. https://doi.org/10.5897/AJB12.1512

Fauziah RH, Kusmiyati F, Anwar S (2019). Lilium longiflorum plant growth with a combination of naphthylacetic acid (NAA) and 6-Benzylamino purine (BAP) in vitro. Journal Tropical Crop Science and Technology 1(2):78-92.

Hoesen DSH (2009). Pembentukan tunas Lilium sp. secara ex vıtro dan ın vıtro (Ex vitro and in vitro shoot formation on Lilium sp.) Jurnal Teknik Lingkungan 10(2):183-193 (Indonesian).

Joshi SK, Dhar U (2009). In vitro propagation from axenic explants of Lilium oxypetalum (D. Don) Baker, an endemic bulbous plant of high-altitude Himalaya. Acta Physiologia Plantarum 31:833-838. https://doi.org/10.1007/s11738-009-0299-y

Kanchanapooma K, Ponpiboona T, Wirakiatb W, Kanchanapoom K (2011). Regeneration of lily (Lilium longiflorum ‘Easter lily’) by callus derived from leaf explants cultured in vitro. Science Asia 37:373-376. https://doi.org/10.2306/scienceasia1513-1874.2011.37.373

Kumar S, Kumar N (2021). Lilium cut flower production under polyhouse. Krishi Udyan Darpan 1(2):66-70.

Kurniati R, Purwito A, Wattimena GA, Marwoto B (2011). Induksi kalus tiga kultivar lili (Lilium sp) dari petal bunga pada beberapa media (Callus induction of three cultivars Lilium sp from petals on several medium). Proceedings Seminar Nasional PERHORTI 2011. Lembang 23-24-November 2011. Pp 1244-1250. (Indonesian)

Lapiz-Culqui YK, Meléndez-Mori JB, Mállap-Detquizán G, Tejada-Alvarado JJ, Vilca-Valqui NC, Hauman-Haman E, Oliva M, Goňas M (2022). In vitro bulbification of five lily varieties: an effective method to produce quality seeds and flowers. International Journal of Agronomy 8775989:1-8. https://doi.org/10.1155/2022/8775989

Lestari NKD, Deswiniyanti NW, Astarini IA, Arpiwi LM (2020). Morphogenesis in vitro flower pedicel of Lilium longiflorum with NAA and BAP. KnE Life Sciences 5(2):18-31. https://doi.org/10.18502/kls.v5i2.6436

Mattjik AA, Sumertajaya IS (2006). Experimental design with SAS and minitab application. IPB Press. Bogor. Pp 276.

Mir JI, Ahmed N, Itoo H, Sheikh MA, Rashid R, Wani SH (2012). In vitro propagation of Lilium (Lilium longiflorum). Indian Journal of Agricultural Sciences 82(5):455-458.

Naeem M, Naveed I, Naqvi SMS, Mahmood T (2013). Standardization of tissue culture conditions and estimation of free scavenging activity in Viola odorata L. Pakistan Journal of Botany 45(1):197-202.

Pramanik D, Rachmawati F (2010). Pengaruh jenis media kultur in vitro dan jenis eksplan terhadap morfogenesis lili oriental (The effect of in vitro culture media and explants on morphogenesis of Oriental lily). Jurnal Hortikultura 20(2):111-119. (Indonesian)

Putri RU, Widiarsih S, Wulandari RA, Sanjaya L (2021). Keragaman Morfologi Tiga Klon Lili (Lilium spp.) pasca Iradiasi Sinar Gama co-60 dalam Kultur in vitro (Morphological variation of the three lilies (Lilium spp.) after gamma ray co-60 irradiation in in vitro condition). Jurnal Ilmiah Aplikasi Isotop dan Radiasi 17(2):105-112. (Indonesian).

Rafiq S, Rather ZA, Bhat RA, Naski IT, Al-Harbi MS, Banday N, Farooq I, Samra BN, Khan MH, Ahmed AF, Andrabi N (2021). Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid cv. 'Ravenna'. Saudi Journal Biological Sciences 28(12):7581-7587. https://doi.org/10.1016/j.sjbs.2021.09.064.

Royandazagh SD (2019). Efficient approaches to in vitro multiplication of Lilium candidum L. with consistent and safe access throughout year and acclimatization of plant under hot-summer Mediterranean (Csa Type) climate. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(3):734-742. https://doi.org/10.15835/nbha7311486

Ruffoni B, Mascarello C, Savona M (2011). Strategies for Lilium propagation: tradition vs. biotech. Acta Horticulturae 900:347-355. https://doi.org/10.17660/ActaHortic.2011.900.44

Saetiew K, Umamanit T (2015). Micropropagation of Lilium formolongo via leaf explants. Journal of Agricultural Technology 11(4):855-862.

Shabur A (2022). Update Harga Bunga Lili/Lily (Bibit Siap Tanam, Potong dan Umbi) [Update Lilium Flower Prices (Seedlings ready Planted, Cut Flower and Bulb)]. Retrieved 2022 July 15 from: https://harga.web.id/harga-bunga-lili-tanaman-bibit.info.

Sharma G, Sharma P (2022). Protected cultivation of Lilium cut flower in the non-traditional regions. Acta Scientific Agriculture 6(6):6-12. https://doi.org/10.31080/ASAG.2022.06.1140

Singh MP (2017). Production and marketing of floriculture in Himachal Pradesh: A paradigm shift. International Journal for Innovative Research in Multidisciplinary Field 3(6):179-187.

Singh N, Singh AV, Singh SP. (2019). Cultivation and market economics of Lilium flowers grown in Kumaun Hills of Uttarakhand. International Journal of Agricultural Sciences 15(1):163-166. https://doi.org/10.15740/HAS/IJAS/15.1/163-166

Tang YP, Liu XQ, Gituru RW, Chen LQ (2010). Callus induction and plant regeneration from in vitro cultured leaves, petioles and scales of Lilium leucanthum (Baker) Baker. Biotechnology and Biotechnological Equipment 24(4):2071-2076. https://doi.org/10.2478/v10133-010-0077-4

Tokgoz HB, Altan F (2020). Callus induction and micropropagation of Lilium candidum L. using stem bulbils and confirmation of genetic stability via SSR-PCR. International Journal of Secondary Metabolite 7(4):286-296. https://doi.org/10.21448/ijsm.753053

Tokotanamanhias (2012). Sentra Produksi Bunga Potong Segar Di Indonesia (Indonesian Production Center of Fresh Cut Flowers). Retrieved 2012 June 19 from: https://tokobungahias.com/sentra-produksi-bunga-potong-segar-di-indonesia/ (Indonesian)

Yan R, Wang C, Wang J, Nie R, Sun H (2020). High-efficiency somatic embryogenesis techniques for different hybrids of cut lilies. Plant Cell, Tissue and Organ Culture 143:145-157. https://doi.org/10.1007/s11240-020-01904-4

Youssef NM, Shaaban SA, Ghareeb ZF, Taha LS (2019). In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. ‘Starfighter’ plants. Bulletin of the National Research Centre 43:211. https://doi.org/10.1186/s42269-019-0246-z

Downloads

Published

2024-02-12

How to Cite

WINARTO, B., KARTIKANINGRUM, S., RACHMAWATI, F., PRAMANIK, D., SHINTIAVIRA, H., & WEGADARA, M. (2024). Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13324. https://doi.org/10.15835/nbha52113324

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha52113324

Most read articles by the same author(s)