Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions

Mehvish RAMZAN; Wang LIHONG; Huma TARIQ; Syed F.A. GILLANI; Saima SHAFIQUE; Adnan RASHEED; Mahmoud F. SELEIMAN

doi:10.15835/nbha51313313

Authors

Mehvish RAMZAN University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
Wang LIHONG Baicheng Normal University, College of Tourism and Geographic Science, Baicheng 137000 (BO)
Huma TARIQ University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
Syed F.A. GILLANI Gansu Agriculture University, Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, 730070 (CN)
Saima SHAFIQUE University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
Adnan RASHEED Hunan Agricultural University, College of Agronomy, 410128, Changsha (CN)
Mahmoud F. SELEIMAN King Saud University, College of Food and Agriculture Sciences, Plant Production Department, Riyadh 11451 (SA)

DOI:

https://doi.org/10.15835/nbha51313313

Keywords:

antioxidant, diversity, flavonoid, phenolic, SDS PAGE

Abstract

Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.

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