Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions


  • Mehvish RAMZAN University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
  • Wang LIHONG Baicheng Normal University, College of Tourism and Geographic Science, Baicheng 137000 (BO)
  • Huma TARIQ University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
  • Syed F.A. GILLANI Gansu Agriculture University, Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, 730070 (CN)
  • Saima SHAFIQUE University of Poonch, Faculty of Agriculture, Department of Plant Breeding and Molecular Genetics, AJK (PK)
  • Adnan RASHEED Hunan Agricultural University, College of Agronomy, 410128, Changsha (CN)
  • Mahmoud F. SELEIMAN King Saud University, College of Food and Agriculture Sciences, Plant Production Department, Riyadh 11451 (SA)



antioxidant, diversity, flavonoid, phenolic, SDS PAGE


Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.


Autran JC, Pogna NE, Kudryavtsev AM, Navilov NI (1995). Use of genetic variation in the improvement of quality in durum wheat. In: Di Fonzo N, Kaan F, Nachit M (Edd). Durum wheat quality in the Mediterranean region. Zaragoza: CIHEAM (Mediterranean Options: Series A. Mediterranean Seminars; n. 22). Seminar on Durum Wheat Quality in the Mediterranean Region, 17-19 Nov 1993, Zaragoza (Spain), pp 173-180.

Ayed S, Karmous C, Slim A, Amara HS (2010). Genetic variation of durum wheat landraces and cultivars using morphological and protein markers. African Journal of Biotechnology 9:8277-8282.

Bartosova ML, Kosik T, Kobida L (2013). Free flavonoid content and antioxidant activity of winter wheat in Sustainable Farming System Journal of Microbiology, Biotechnology and Food Sciences 2:2099-2107.

Belcredi NB, Ehrenbergerova J, Fiedlerova V, Belakova S, Vaculova K (2010). Antioxidant vitamins in barley green biomass. Journal of Agriculture and Food Chemistry 58:11755-11761.

Benmoussa M, Vezina LP, Page M, Yelle S, Laberge S (2000). Genetic polymorphism in low-molecular-weight gluten in genes from Triticum aestivum, variety Chinese Spring. Theoretical and Applied Genetics 100(5):789-793.

Bietz JA, Wall JS (1972). Wheat gluten subunits: Molecular weights determined by sodium sulfate-polyacrylamide gel electrophoresis. Cereal Chemistry 49:416-430.

Chang CC, Yang MH, Wen HM, Chern JC (2002). Estimation of total flavonoids contents in propils by two complementary colorimetric methods. Analytical Journal of Food Drug 10:178-182.

Ehrenbergerova J, Belcredi BN, Kopacek J, Melisova L, Hrstkova P, Macuchov S, Vaculova K, Paulickova I (2009). Antioxidant enzymes in barley green biomass. Plant Foods for Human Nutrition 64:122128.

Feillet P (2000). The grain of wheat; composition and utilization. Edition INRA, France. Pp 308.

Ghafoor K and Choi YH, Jeon JY, Jeo IH (2009). Optimization of ultrasound assisted extraction of phenolic compounds and antioxidants from grape seeds. Journal of Agriculture and Chemistry 57(11):4988-4994.

Gianibelli MC, Gupta RB, Lafiandra D, Margiotta B, MacRitchie F (2001). Polymorphism of high Mr. glutenin subunits in Triticumtauschii: characterization by chromatography and electrophoretic methods. Journal of Cereal Science 33:39-52.

Hammer O, Harper DAT, Ryan PD (2001). PAST: Paleantological statistics software package for education and data analysis. Palaeontologia Electronica 4(1):9.

Hatano T, Kagawa H, Yasuhara T, Okuda T (1988). Two new flavonoids and other constituents in licorice root; their relative astringency and radical scavenging effects. Chemical and Pharmaceutical Bulletin 36:2090-2097.

Koleva II, Beek TAV, Linseen JPH, Groot AD, Evstatieva LN (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13: 8-17.

Liu RH (2007). Whole grain phytochemical and health. Journal of Cereal Science 46:207-219.

Nakamura H (2001). Genetic diversity of high-molecular-weight gluten in subunit compositions in landraces of hexaploid wheat from Japan. Euphotic 120:227-234.

Payne PI, Holt LM, Jackson EA, Law CN (1984). Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Philosophical Transactions of the Royal Society London 304:1120.

Picard P, Greneche DMB, Zivy M (2005). Potential of two-dimensional electrophoresis in routine identification of closely related durum wheat lines. Electrophoresis 18:174-181.

Porto CD, Calligaris S, Celloti E, Nicoli MC (2000). Antiradical properties of commercial cognacs assessed by the DPPH test. Journal of Agriculture and Food Chemistry 48:4241-4245.

Ruiz M, Quijano MR, Metakovsky EV, Vazquez F, Carrillo JM (2002). Polymorphism variation and genetic identity of Spanish common wheat germplasm based on the gliadin alleles. Field Crops Research 185-196.

Sneath PHA, Sokal RR (1973). The principles and practices of numerical classification. Numerical Taxonomy: W. F and Co. Freeman, San Francisco.

Starovicova M, Galova Z, Knoblochova H (2003). Identification of glutenin markers in cultivars of three wheat species. Czech Journal of Plant Breeding 39:51-57.



How to Cite

RAMZAN, M., LIHONG, W., TARIQ, H., GILLANI, S. F., SHAFIQUE, S., RASHEED, A., & SELEIMAN, M. F. (2023). Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(3), 13313.



Research Articles
DOI: 10.15835/nbha51313313

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