Pre-harvest application of proline, methionine, and melatonin improves shelf-life and maintains nutritional quality of Brassica oleracea florets during cold storage


  • Hossam S. EL-BELTAGI King Faisal University, College of Agriculture and Food Sciences, Agricultural Biotechnology Department, Al-Ahsa 31982; Cairo University, Faculty of Agriculture, Biochemistry Department, Giza 12613 (SA)
  • Emad A. ABDELDAYM Cairo University, Faculty of Agriculture, Vegetable Crops Department, Giza, 12613 (EG)
  • Hoda A.S. FARAG King Faisal University, Food and Nutrition Department, College of Agriculture and Food Sciences, Al-Ahsa 31982 (SA)
  • Samar M.A. DOKLEGA Mansoura University, Faculty of Agriculture, Vegetables and Floriculture Department, Mansoura 35516 (EG)
  • Mahmoud A.M. ABD EL-HADY Damietta University, Faculty of Agriculture, Horticulture Department, Damietta 34517 (EG)
  • Suzy M. ABDELAZIZ Horticulture Research Institute, Cross Pollinated Vegetable Research Department, 12611, ARC (EG)
  • Mohamed M. EL-MOGY Cairo University, Faculty of Agriculture, Vegetable Crops Department, Giza, 12613 (EG)
  • Ayesha MARYAM University of Agriculture, Institute of Horticultural Sciences, Postharvest Research and Training Centre, Faisalabad (PK)
  • Hemat A. EL-BAUOME Mansoura University, Faculty of Agriculture, Vegetables and Floriculture Department, Mansoura 35516 (EG)



antioxidants, browning index, cauliflower plants, postharvest quality, storability


The reduction in shelf-life and nutritional value of cauliflower florets are the most vital problems during cold storage. This research was performed to explore the impact of the pre-harvest foliar implementation of proline (PR), methionine (MT), and melatonin (ML), at a rate of 25 mg.L−1, on shelf-life and active composites of cauliflower florets (cv. ‘Arasya’) stored at 10 °C for 14 d. The obtained results exhibited that florets from treated plants with PR, MT, and ML were lower in water loss, electrolyte leakage, browning index, and titratable acidity (TA) compared with untreated plants, at the end of storage. Furthermore, these pre-harvest treatments significantly slow down the loss of total sugar content, reducing sugar, total soluble solid (TSS), glucosinolates concentration (Gly), total phenols (TP), vitamin C (VC), and antioxidant activity (DPPH) than control samples. After 14 days of storage, the application of PR, MT, and ML significantly improved the activity levels of phenylalanine ammonia-lyase (PAL), and catalase (CAT) as well as declined the polyphenol oxidase (PPO), peroxidase (POD) activates in the florets during the cold storage. Therefore, the pre-harvest application of PR, MT, and ML might be promising substances to keep the nutritional quality of cauliflower forests and reduce the browning index during cold storage.

Author Biography

Hossam S. EL-BELTAGI, King Faisal University, College of Agriculture and Food Sciences, Agricultural Biotechnology Department, Al-Ahsa 31982; Cairo University, Faculty of Agriculture, Biochemistry Department, Giza 12613

Department of Biochemistry, Faculty of Agriculture, Cairo University


Aebi H (1984). Catalase in vitro. Methods in Enzymology 105:121-126.

Afify AE-MMR, El-Beltagi HS, Aly AA, El-Ansary AE (2012a). Antioxidant enzyme activities and lipid peroxidation as biomarker for potato tuber stored by two essential oils from caraway and clove and its main component carvone and eugenol. Asian Pacific Journal of Tropical Biomedicine 2:S772-S780.

Afify AE-MMR, El-Beltagi HS, Aly AA, El-Ansary AE (2012b). The impact of γ-irradiation, essential oils and iodine on biochemical components and metabolism of potato tubers during storage. Notulae Botanicae Horti Agrobotanici Cluj Napoca 40(2):129-139.

Afify AE-MMR, El-Beltagi HS, Aly AA, El-Ansary AE. (2012c). Antioxidant enzyme activities and lipid peroxidation as biomarker compounds for potato tuber stored by gamma radiation. Asian Pacific Journal of Tropical Biomedicine 2(3):S1548-S1555.

Abdel-Hakim SG, Shehata ASA, Moghannem SA, Qadri M, El-Ghany, MFA, Abdeldaym EA, Darwish OS (2023). Nanoparticulate fertilizers increase nutrient absorption efficiency and agro-physiological properties of lettuce plant. Agronomy 13:691.

Aghdam MS, Luo ZS, Li L, Jannatizadeh A, Fard JR, Pirzad F (2020). Melatonin treatment maintains nutraceutical properties of pomegranate fruits during cold storage. Food Chemistry 303:125385.

Al-Dair M, Pathare PB, Al-Yahyai R (2021). Chemical and nutritional quality changes of tomato during postharvest transportation and storage. Journal of the Saudi Society of Agricultural Sciences 20(6):401-408.

Ali S, Khan AS, Malik AU, Shaheen T, Shahid M (2018). Pre-storage methionine treatment inhibits postharvest enzymatic browning of cold stored ‘Gola’litchi fruit. Postharvest Biology and Technology 1:100-106.

Ao C, Li A, Elzaawely AA, Xuan TD, Tawata S (2008). Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract. Food Control 19:940-948.

Aly AA, El-Desouky W, Abou El-Leel O F (2022). Micropropagation, phytochemical content and antioxidant activity of gamma irradiated blackberry (Rubus fruticosus L.) plantlets. In Vitro Cellular & Developmental Biology-Plant 58:457-46.

Bahmani R, Razavi F, Mortazavi SN, Gohari G, Juárez-Maldonado A (2022). Evaluation of proline-coated chitosan nanoparticles on decay control and quality preservation of strawberry fruit (cv. Camarosa) during cold storage. Horticulturae 8:648.

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-254.

Cliffe-Byrnes V, O’Beirne D (2008). Effects of washing treatment on microbial and sensory quality of modified atmosphere (MA) packaged fresh sliced mushroom (Agaricus bisporus). Postharvest Biology and Technology 48(2):283-294.

Darwish OS, Ali MR, Khojah E, Samra BN, Ramadan KMA, El-Mogy MM (2021). Pre-harvest application of salicylic acid, abscisic acid, and methyl jasmonate conserve bioactive compounds of strawberry fruits during refrigerated storage. Horticulturae 7:568.

EL-Bauome HA, Abdeldaym EA, Abd El-Hady MAM, Darwish DBE, Alsubeie MS, El-Mogy MM, … Doklega SMA (2022). Exogenous proline, methionine, and melatonin stimulate growth, quality, and drought tolerance in cauliflower plants. Agriculture 12:1301.

El-Beltagi HS, Al-Otaibi HH, Ali MR (2023). A new approach for extending shelf-life of pomegranate arils with combined application of salicylic acid and methyl jasmonate. Horticulturae 9:225.

El-Beltagi HS, El-Yazied AA, El-Gawad HGA, Kandeel M, Shalaby TA, Mansour AT, … Ibrahim MFM (2023) Synergistic impact of melatonin and putrescine interaction in mitigating salinity stress in snap bean seedlings: reduction of oxidative damage and inhibition of polyamine catabolism. Horticulturae 9:285.

El-Beltagi HS, Mohamed HI, Abdelazeem AS, Youssef R, Safwat G (2018). GC-MS analysis, antioxidant, antimicrobial and anticancer activities of extracts from Ficus sycomorus fruits and leaves. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(2):493-505.

Elsayed N, Hassan AA, Abdelaziz SM, Abdeldaym EA, Darwish OS (2020). Effect of whey protein edible coating incorporated with mango peel extract on postharvest quality, bioactive compounds and shelf life of broccoli. Horticulturae 8:770.

Fatima F, Basit A, Younas M, Shah ST, Sajid M, Aziz I, Mohamed, HI (2023). Trends in potassium permanganate (ethylene absorbent) management strategies: towards mitigating postharvest losses and quality of mango (Mangifera indica L) fruit. Food and Bioprocess Technology 1-12.

Galani JHY, Mankad PM, Shah AK, Patel NJ, Acharya RR, Talati JG (2017). Effect of storage temperature on vitamin C, total phenolics, UPLC phenolic acid profile and antioxidant capacity of eleven potatoes (Solanum tuberosum) varieties. Horticultural Plant Journal 3(2):73-89.

Gao H, Cheng N, Zhou J, Wang B, Deng J, Cao W (2011). Antioxidant activities and phenolic compounds of date plum persimmon (Diospyros lotus L.) fruits. Journal of Food Science and Technology 51:950.

Gohari G, Molaei S, Kheiry A, Ghafouri M, Razavi F, Lorenzo JM, Juárez-Maldonado A (2021). Exogenous application of proline and l-cysteine alleviates internal browning and maintains eating quality of cold stored flat ‘Maleki’ peach fruits. Horticulturae 7:469.

Han X, Wang X, Shen C, Mo Y, Tian R, Mao L, Luo Z, Yang H (2022). Exogenous ABA promotes aroma biosynthesis of postharvest kiwifruit after low-temperature storage. Planta 554:82.

Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012). Role of proline under changing environments: a review. Plant Signal Behaviour 7(11):1456-1466.

Hernández-Ruiz J, Ruiz-Cano D, Giraldo-Acosta M, Cano A, Arnao MB (2022). Melatonin in Brassicaceae: Role in postharvest and interesting phytochemicals. Molecules 27:1523.

Hossain MA, Kumar V, Burritt DJ, Fujita M, Mäkelä P (2019). Osmoprotectant-mediated abiotic stress tolerance in plants. In: Proline Metabolism and Its Functions in Development and Stress Tolerance. Springer Nature: Cham, Switzerland, pp 41-72.

Huyskens-Keil S, Hassenberg K, Herppich WB (2012). Impact of postharvest UV-C and ozone treatment on textural properties of white asparagus (Asparagus officinalis L.). Journal of Applied Botany and Food Quality 84:229.

Ismail HA, Richard I, Ramaiya SD, Zakaria MH, Lee SY (2023). Browning in relation to enzymatic activities and phytochemical content in terap peel (Artocarpus odoratissimus Blanco) during postharvest ripening. Horticulturae 9:57.

Jannatizadeh A (2019). Exogenous melatonin applying confers chilling tolerance in pomegranate fruit during cold storage. Scientia Horticulturae 246:544-549.

Jiang B, Fang X, Fu D, Wu W, Han Y, Chen H, Liu R, Gao H (2022). Exogenous salicylic acid regulates organic acids metabolism in postharvest blueberry fruit. Frontiers in Plant Science 13.

Kapusta-Duch J, Szelag-Sikora A, Sikora J, Niemiec M, Grodek-Szostak Z, Kubon M, Leszczynska T, Borczak B (2019). Health promoting properties of fresh and processed purple cauliflower. Sustainability 11:4008.

Khan S, Yu H, Li Q, Gao Y, Sallam BN, Wang H, Liu P, Jiang W (2019). Exogenous application of amino acids improves the growth and yield of lettuce by enhancing photosynthetic assimilation and nutrient availability. Agronomy 9:266.

Lai T, Wang Y, Li B, Qin G, Tian S (2011). Defense responses of tomato fruit to exogenous nitric oxide during postharvest storage. Postharvest Biology and Technology 62(2):127-132.

Liu P, Xu N, Liu R, Liu J, Peng Y, Wang Q (2022). Exogenous proline treatment inhibiting enzymatic browning of fresh-cut potatoes during cold storage. Postharvest Biology and Technology 184:111754.

Meng Z, Dong T, Malik AU, Zhang S, Wang Q (2021). Harvest maturity affects the browning of fresh-cut potatoes by influencing contents of amino acids. Postharvest Biology and Technology 173:111404.

Meščić Macan A, Gazivoda Kraljević T, Raić-Malić S (2019). Therapeutic perspective of vitamin c and its derivatives. Antioxidants 8:247.

Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3):426-8.

Miranda S, Vilches P, Suazo M, Pavez L, García K, Méndez MA, … Del Pozo T (2020) Melatonin triggers metabolic and gene expression changes leading to improved quality traits of two sweet cherry cultivars during cold storage. Food Chemistry 319:126360.

Mohamed MK, Abouel-Yazeed AM, Abdalla AE, Maraei RW, Aly AA (2023). Quality characteristic of white button mushrooms (Agaricus bisporus) affected by gamma irradiation and volatile oils during storage. Radiochimica Acta.

Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880.

Nandi A, Yan LJ, Jana CK, Das N (2019). Role of catalase in oxidative stress- and age-associated degenerative diseases. Oxidative Medicine and Cellular Longevity 11:9613090.

Nasrin TAA, Yasmin L, Arfin MS, Rahman MA, Molla MM, Sabuz AA, Afroz M (2022). Preservation of postharvest quality of fresh cut cauliflower through simple and easy packaging techniques. Applied Food Research 2:100125.

Njie A, Zhang W, Dong X, Lu C, Pan X, Liu Q (2022). Effect of melatonin on fruit quality via decay inhibition and enhancement of antioxidative enzyme activities and genes expression of two mango cultivars during cold storage. Foods 11:3209.

Onik JC, Wai SC, Li A, Lin Q, Sun Q, Wang Z, Duan Y (2021). Melatonin treatment reduces ethylene production and maintains fruit quality in apple during postharvest storage. Food Chemistry 337:127753.

Owolabi IO, Songsamoe S, Khunjan K, Matan N (2021). Effect of tapioca starch coated-rubberwood box incorporated with essential oils on the postharvest ripening and quality control of mangosteen during transportation. Food Control 126:108007.

Perdones Á, Escriche I, Chiralt A, Vargas M (2016) Effect of chitosan-lemon essential oil coatings on volatile profile of strawberries during storage. Food Chemistry 197: 979-986.

Picchi V, Fibiani M, Scalzo RL (2020). Cauliflower in nutritional composition and antioxidant properties of fruits and vegetables. Academic Press: Cambrige, MA, USA 1:19-32.

Qi X, Zhao J, Jia Z, Cao Z, Liu C, Li J, Su Y, Pan Y, He C, Xu Y, Qin G (2022). Potential metabolic pathways and related processes involved in pericarp browning for postharvest pomegranate fruits. Horticulturae 8(10):924.

Sati H, Khandelwal A, Pareek S (2023). Effect of exogenous melatonin in fruit postharvest, crosstalk with hormones, and defense mechanism for oxidative stress management. Food Frontiers.

Shehata SA, Abdeldaym EA, Ali MR, Mohamed RM, Bob RI, Abdelgawad KF (2020). Effect of some citrus essential oils on post-harvest shelf life and physicochemical quality of strawberries during cold storage. Agronomy 10:1466.

Slominski BA, Campbell LD (1987). Gas Chromatographie determination of indole glucosinolates—A re-examination. Journal of the Science of Food and Agriculture 40:131-143.

Sohail M, Wills, RB, Bowyer MC, Pristijono P (2021). Impact of exogenous arginine, cysteine and methionine on the postharvest senescence of six green leafy vegetables. Journal of Horticulture and Postharvest Research 4(3):253-262.

Southerton SG, Deverall BJ (1990). Changes in phenylalanine ammonia lyase and peroxidase activities in wheat cultivars expressing resistance to the leaf- rust fungus. Plant Pathology 39:223-230.

Wang SY, Shi XC, Liu FQ, Laborda P (2021.) Effects of exogenous methyl jasmonate on quality and preservation of postharvest fruits: A review. Food Chemistry 353:129482.

Xu T, Chen Y, Kang H (2019). Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Frontiers in Plant Science 10:1388.

Yang X, Chen J, Ma Y, Huang M, Qiu T, Bian H, Han N, Wang J (2022). Function, mechanism, and application of plant melatonin: An update with a focus on the cereal crop, barley (Hordeum vulgare L.). Antioxidants 11(4):634.

You J, Zhang Y, Liu A, Li D, Wang X, Dossa K, Zhou R, Yu J, Zhang Y, Wang L (2019). Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress. BMC Plant Biology 19:267.

Zhan L, Hu J, Pang L, Li Y, Shao J (2014). Light exposure reduced browning enzyme activity and accumulated total phenols in cauliflower heads during cool storage. Postharvest Biology and Technology 88:17-20.

Zhang Y, Huber DJ, Hu M, Jiang G, Gao Z, Xu X, Jiang Y, Zhang Z (2018). Delay of postharvest browning in litchi fruit by melatonin via the enhancing of antioxidative processes and oxidation repair. Journal of Agricultural and Food Chemistry 66(28):7475-7484.



How to Cite

EL-BELTAGI, H. S., ABDELDAYM, E. A., FARAG, H. A., DOKLEGA, S. M., ABD EL-HADY, M. A., ABDELAZIZ, S. M., EL-MOGY, M. M., MARYAM, A., & EL-BAUOME, H. A. (2023). Pre-harvest application of proline, methionine, and melatonin improves shelf-life and maintains nutritional quality of Brassica oleracea florets during cold storage. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(2), 13218.



Research Articles
DOI: 10.15835/nbha51213218

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