Methodological contribution on seed germination and seedling initial growth tests in wild plants

Authors

  • Božena ŠERÁ Comenius University Bratislava, Faculty of Natural Sciences, Department of Environmental Ecology and Landscape Management, Mlynská dolina, Ilkovičova 6, Bratislava (SK) https://orcid.org/0000-0001-5275-1015

DOI:

https://doi.org/10.15835/nbha51213164

Keywords:

germination rate, germination index, mean germination time, R/S ratio, seed data analysis, seedling vitality index

Abstract

The text is intended for biologists and ecologists working with wild plant species. The article draws attention to possible methodological inaccuracies in determining seed germination and in various attempts to manipulate the seeds of wild plant species. The difference between seed germination in the laboratory and in the wild (in the field), basic principles of seed collection or selection, selection of test sample, basic parameters related to seed germination (seed germination, germination rate, mean germination time, germination index), and initial growth seedlings (seedling vitality index, R/S ratio) are described. The text aims to methodically clarify experiments related to seed germination and initial seedling growth, so different wild plant species can be better compared with each other.

References

Abdul-Baki AA, Anderson JD (1972). Physiological and biochemical deterioration of seeds. In: Kozlowski TT (Ed). Seed Biology. New York, Academic Press, pp 283-315.

Agusti-Brisach C, Perez-Sierra A, Armengol J, Garcia-Jimenez J, Berbegal M (2012). Efficacy of hot water treatment to reduce the incidence of Fusarium circinatum on Pinus radiata seeds. Forestry 85:629-635. https://doi.org/10.1093/forestry/cps074

Allen T, Enebak S, Carey W (2004). Evaluation of fungicides for control of species of Fusarium on longleaf pine seed. Crop Protection 23:979-982. https://doi.org/10.1016/j.cropro.2004.02.010

Aniszewski T, Haikonen J, Helwig B, Konert G, Oleksinska Z, Stenman A, Ylinampa T (2012). Vigor, vitality and seed dormancy of Avena sativa cultivars in a long-term experiment. Journal of Applied Botany and Food Quality 85:150-158.

Baalbaki R, Elias S, Marcos-Filho J, McDonald MB (2009). Seed vigour testing handbook. Association of Official Seed Analysts, Ithaca, NY, USA.

Baskin CC, Baskin JM (2014). Seeds: ecology, biogeography and evolution of dormancy and germination. 2nd ed., Aca-demic Press, New York, NY, USA.

Basra AS (1995). Seed Quality: Basic Mechanisms and Agricultural Implications. Haworth Press, N.Y.

Belgacem AO, Neffati M, Papanastasis VP, Chaieb M (2006). Effects of seed age and seeding depth on growth of Stipa lagascae R. & Sch seedlings. Journal of Arid Environment 65:682-687. https://doi.org/10.1016/j.jaridenv.2005.10.001

Bewley JD, Bradford K, Hilhorst H, Nonogaki H (2013). Physiology of development, germination and dormancy. 3rd Ed., Springer.

Borg SJ (2005). Dormancy and germination of six Rhinanthus species in relation to climate. Folia Geobotanica 40:243-260. https://doi.org/10.1007/BF02803238

Christofoletti CA, Escher JP, Correia JE, Marinho JFU, Fontanetti CS (2013). Sugarcane vinasse: Environmental im-plications of its use. Waste Management 33:2752-2761. https://doi.org/10.1016/j.wasman.2013.09.005

Copeland LO, McDonald MB (1995). Principles of Seed Science and Technology. Seed Enhancements. Chapman and Hall, New York.

Deno NC (1993). Seed germination theory and practice. 2nd ed., Norman C. Deno, State College, USA.

de Vitis M, Hay FR, Dickie JB, Trivedi C, Choi J, Fiegener R (2020). Seed storage: Maintaining seed viability and vigor for restoration use. Restoration Ecology 28:S249-S255. https://doi.org/10.1111/rec.13174

El-Keblawy A, Shaltout KH, Lovett-Doust J, Ramadan A (1997). Population dynamics of an Egyptian desert shrub, Thyme-laea hirsute. Canadian Journal of Botany 75:2027-2037.

Ellis RH, Roberts EH (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9:373-409.

Fenner M, Thompson K (2005). The ecology of seeds. Cambridge University Press, Cambridge.

Frischie S, Miller AL, Pedrini S, Kildisheva OA (2020). Ensuring seed quality in ecological restoration: native seed cleaning and testing. Restauration Ecology 28:239-248. https://doi.org/10.1111/rec.13217

Gilbert GS, Diaz A, Bregoff HA (2023). Seed disinfestation practices to control seed-borne fungi and bacteria in home production of sprouts. Foods 12:747. https://doi.org/10.3390/foods12040747

Gresta F, Avola G, Abbate V (2007). Germination ecology of Scorpiurus subvillosus L. seeds: the role of temperature and storage time. Plant Ecology 190:123-130. https://doi.org/10.1007/s11258-006-9195-3

Hafez M, Popov AI, Rashad M (2021): Integrated use of bio-organic fertilizers for enhancing soil fertility–439 plant nutri-tion, germination status and initial growth of corn (Zea mays L.). Environmental Technology and Innovation 21:101329. https://doi.org/10.1016/j.eti.2020.101329

Hampton J (1993). The ISTA perspective of seed vigor testing. Journal of Seed Technology 17:105-109.

Hampton JG, Coolbear P (1990). Potential versus actual seed performance - can vigour testing provide an answer? Seed ScienceTechnology 18:215-228.

Hampton JH, Tekrony DM (1995). Handbook of Vigor Test Methods. 3rd ed. International Seed Testing Association, Zurich.

Hendry GAF, Grime JP (1993). Methods in Comparative Plant Ecology. A laboratory manual. Chapman and Hall, Sheffield.

Justice OL, Bass LN (1978). Principles and Practices of Seed Storage. U.S. Government Printing Office, Washington, D.C.

Kader MA (2005). A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Journal and Proceedings of the Royal Society NSW 138:65-75.

Khan AZ, Shah P, Mohd F, Khan H, Amanullah PS, Nigar S, Khalil SK, Zubair M (2010). Vigor tests used to rank seed lot quality and predict field emergence in beat. Pakistan Journal of Botany 42:3147-3155.

Kim SH, Choe ZR, Kang JH, Copeland LO, Elias SG (1994). Multiple seed vigour indices to predict field emergence and performance of barley. Seed Science and Technology 22:29-38.

Knevel IC, Bekker RM, Kunzmann D, Stadler M, Thompson K (2005). The LEDA Traitbase. Collecting and Measuring Standards of Life-history Trans of the Northwest European Flora. Scholma Druk B.V., Bedum, Netherlands.

Kozlowski TT (1999). Soil compaction and growth of woody plants. Scandinavian Journal of Forest Research 14:596-619. https://doi.org/10.1080/02827589908540825

Martin-Garcia J, Zas R, Solla A, Woodward S, Hantula J, Vainio EJ, ... Diez JJ (2019). Environmentally friendly methods for controlling pine pitch canker. Plant Pathology 68:843-860. https://doi.org/10.1111/ppa.13009

Meloun M, Militký J (2006). Kompendium statistického zpracování dat. Metody a řešené úkoly. Academia, Praha.

Milton JS (1992). Statistical Methods in the Biological and Health Science. 2nd ed., Mc Graw Hill, New York.

Orchard T (1977). Estimating the parameters of plant seedling emergence. Seed Science and Technology 5:61-69.

Powell AA, Haigh R, Phillips G, Tonkin HGB, Wheaton OE (1984). Assessment of repeatability of the controlled dete-rioration vigour test both within and between laboratories. Seed Science and Technology 12:421-427.

Ranal MA, de Santana DG (2006). How and why to measure the germination process? Brazilian Journal of Botany 29:1-11. https://doi.org/10.1590/S0100-84042006000100002

Schutte BJ, Regnier EE, Harrison SK (2008). The association between seed size and seed longevity among maternal families in Ambrosia trifida L. populations. Seed Science Research 18(4):201-211. https://doi.org/10.1017/S0960258508082974

Šerá B, Kraus K, Hnilička F, Medvecká V, Zahoranová A, Šerý M (2021a). Effect of atmospheric non-thermal plasma treatment by DCSBD apparatus on sugar beet seeds. Romanian Reports in Physics 73:602.

Šerá B, Šerý M, Straňák V, Špatenka P, Tichý M (2009). Does cold plasma affect breaking dormancy and seed germination? A study on seeds of Lamb’s Quarters (Chenopodium album agg.). Plasma Science and Technology 11:750-754.

Šerá B, Šerý M, Zahoranová A, Tomeková J (2021b). Germination improvement of three pine species (Pinus) after diffuse coplanar surface barrier discharge plasma treatment. Plasma Chemistry and Plasma Processing 41:211-226. https://doi.org/10.1007/s11090-020-10128-5

Šerý M, Zaharonová A, Kerdík A, Šerá B (2020). Seed germination of black pine (Pinus nigra Arnold) after diffuse coplanar surface barrier discharge plasma treatment. IEEE Transactions on Plasma Science 48:939-945. https://doi.org/10.1109/TPS.2020.2981600

Soares PG, Rodrigues RR (2008). Direct seeding of forest leguminous trees: rhizobia inoculation effect on plantlet emergence and early growth in field conditions. Scientific Forestry 36:115-121. https://doi.org/10.21750/REFOR.4.07.46

Uddin S, Ullah S, Nafees M (2021). Effect of seed priming on growth and performance of Vigna radiata L. under induced drought stress. Journal of Agriculture and Food Research 4:100140. https://doi.org/10.1016/j.jafr.2021.100140

Vieira BGTL, Barbosa GF, Barbosa RM, Vieira RD (2013). Structural changes in soybean seed coat due to harvest time and storage. Journal of Food, Agriculture and Environment 11:625-628.

Verma SS, Verma U, Tomer RPS (2003). Studies on seed quality parameters in deteriorating seeds in Brassica (Brassica campestris). Seed Science and Technology 31:389-396. https://doi.org/10.15258/sst.2003.31.2.15

Published

2023-06-20

How to Cite

ŠERÁ, B. (2023). Methodological contribution on seed germination and seedling initial growth tests in wild plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(2), 13164. https://doi.org/10.15835/nbha51213164

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DOI: 10.15835/nbha51213164