Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+


  • Hana BOKRI University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis (TN)
  • Samiha KAHLAOUI University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis (TN)
  • Kheiria HCINI University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis (TN)
  • Manel DHOUEIBI University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis (TN)
  • Fethia HARZALLAH-SKHIRI University of Monastir, High Institute of Biotechnology of Monastir, Laboratory of Bioresources: Integrative Biology and Valorization (LR14-ES06), 5000 Monastir (TN)
  • Sondes STAMBOULI-ESSASSI University of Tunis El Manar, Faculty of Sciences of Tunis, Laboratory of Biodiversity, Biotechnology and Climate Change (LR11-ES09), 1060 Tunis (TN)



callogenesis, Limbarda crithmoïdes L, organogenesis, potassium, sodium


The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites.


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How to Cite

BOKRI, H., KAHLAOUI, S., HCINI, K., DHOUEIBI, M., HARZALLAH-SKHIRI, F., & STAMBOULI-ESSASSI, S. (2023). Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(2), 13147.



Research Articles
DOI: 10.15835/nbha51213147