Transcriptomic analysis reveals transcription factors involved in vascular bundle development and tissue maturation in ginger rhizomes (Zingiber officinale Roscoe)

Authors

  • Zhengyan CAO Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei (CN)
  • Ning TANG Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing; Chongqing Key Laboratory of Economic Plant Biotechnology, Chongqing 400000 (CN)
  • Zexiong CHEN Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing; Chongqing Key Laboratory of Economic Plant Biotechnology, Chongqing 400000 (CN)
  • Peiyin WU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei (CN)
  • Jiarui ZHENG Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei (CN)
  • Jiabao YE Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei (CN)
  • Yanni LIU Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing (CN)
  • Yang HU Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing (CN)
  • Li ZHANG Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing (CN)
  • Xiaofan SUN Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing (CN)
  • Zhenqi LIU Chongqing University of Arts and Sciences, College of Landscape Architecture and Life Science, Yongchuan 402160, Chongqing (CN)
  • Feng XU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei (CN)

DOI:

https://doi.org/10.15835/nbha51213131

Keywords:

ginger, rhizome maturation, transcription factor, transcriptomic analysis, vascular bundle development

Abstract

Ginger (Zingiber officinale Roscoe) is an important vegetable with medicinal value. Rhizome development determines ginger yield and quality. However, little information is available about the molecular features underlying rhizome expansion and maturation. In this study, we investigated anatomy characteristics, lignin accumulation and transcriptome profiles during rhizome development. In young rhizomes, the vascular bundle (VB) was generated with only vessels in it, whereas in matured rhizomes, three to five layers of fibre bundle in the xylem were formed, resulting in VB enlargement. It indicates VB development favouring rhizome swelling. With rhizome matured, the lignin content was remarkably elevated, thus facilitating tissue lignification. To explore the regulators for rhizome development, nine libraries including ginger young rhizomes (GYR), growing rhizomes (GGR), and matured rhizomes (GMR) were established for RNA-Seq, a total of 1264 transcription factors (TFs) were identified. Among them, 35, 116, and 14 differentially expressed TFs were obtained between GYR and GGR, GYR and GMR, and GGR and GMR, respectively. These TFs were further divided into three categories. Among them, three ZobHLHs (homologs of Arabidopsis LHW and AtbHLH096) as well as one DIVARICATA homolog in ginger might play crucial roles in controlling VB development. Four ZoWRKYs and two ZoNACs might be potential regulators associated with rhizome maturation. Three ZoAP2/ERFs and one ZoARF might participate in rhizome development via hormone signalling. This result provides a molecular basis for rhizome expansion and maturation in ginger.

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Published

2023-06-07

How to Cite

CAO, Z., TANG, N., CHEN, Z., WU, P., ZHENG, J., YE, J., LIU, Y., HU, Y., ZHANG, L., SUN, X., LIU, Z., & XU, F. (2023). Transcriptomic analysis reveals transcription factors involved in vascular bundle development and tissue maturation in ginger rhizomes (Zingiber officinale Roscoe). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(2), 13131. https://doi.org/10.15835/nbha51213131

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Vol. 51 No. 2 (2023)

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Research Articles
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DOI: 10.15835/nbha51213131

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Copyright (c) 2023 Zhengyan CAO, Ning TANG, Zexiong CHEN, Peiyin WU, Jiarui ZHENG, Jiabao YE, Yanni LIU, Yang HU, Li ZHANG, Xiaofan SUN, Zhenqi LIU, Feng XU

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