Incompatible pollen tubes in the quince style and their impact on fertilization success
DOI:
https://doi.org/10.15835/nbha51213083Keywords:
Cydonia oblonga, fluorescence microscopy, open pollination, self-(in) compatibility, self-pollinationAbstract
Self-incompatibility presents one of the challenges in modern fruit production. It can be correlated with a lower yield of self-incompatible cultivars which also demand the planting of the pollinisers. The aim of this work was to investigate the phenomenon of incompatibility and its influence on fertilization success in quince (Cydonia oblonga Mill.), using the cultivars ‘Leskovacka’, ‘Vranjska’, ‘Morava’, ‘Pazardzijska’, ‘Hemus’, ‘Asenica’, ‘Portugal’ and ‘Triumph’. Incompatible pollen tubes were determined by using fluorescence microscopy. In two types of pollination (self-pollination and open pollination) pollen tubes showed signs of incompatibility, mostly in the upper third of the style. The most common sign of incompatibility is the formation of swelling at the tip of a pollen tube. Also, sometimes twisted, bifurcated, and short and thickened pollen tubes along their entire length are formed. The incompatibility was significantly more pronounced in the self-pollination than in the open pollination variant in all tested cultivars. The highest number of incompatible pollen tubes in both pollination types was present in the cultivar ‘Pazardzijska’, while the lowest number was present in the cultivars ‘Leskovacka’ and ‘Vranjska’. The appearance of incompatibility affected the degree of fertilization in quince. The results showed that only ‘Leskovacka’ and ‘Vranjska’ are self-compatible, while other studied cultivars (‘Morava’, ‘Pazardzijska’, ‘Hemus’, ‘Asenica’, ‘Portugal’ and ‘Triumph’) are self-incompatible. The results provide a good background for the future research of reproductive biology and also for adequate management of the quince orchards.
References
Adachi Y, Komori S, Hoshikawa Y, Tanaka N, Abe K, Bessho H, Watanabe M, Suzuki A (2009). Characteristics of fruiting and pollen tube growth of apple autotetraploid cultivars showing self-compatibility. Journal of the Japanese Society for Horticultural Science 78(4):402-409.
Akbari H, Qorbani E (2011). Investigation of self-incompatibility and determination of the best pollinizer for Isfahan Commercial Quinceý.Seed and Plant Improvement Institute.
Alonso JM, Sociasi Company R (2005а). Differential pollen tube growth in inbred self-compatible almond genotypes. Euphytica 144:207-213. https://doi.org/10.1007/s10681-005-5813-8
Alonso JM, Sociasi Company RS (2005b). Self-incompatibility expression in self-compatible almond genotypes may be due to inbreeding. Journal of the American Society for Horticultural Science 130(6):865-869.https://doi.org/10.21273/JASHS.130.6.865
Andrés MV, Durán JM (1998). Self-incompatibility in Spanish clones of apricot (Prunus armeniaca L.) tree. Euphytica 101:349-355.https://doi.org/10.1023/A:1018325517208
Benedek P, Szabó T, Nyéki J (2000). The effect of the limitation of insect pollination period on the fruit set and yield of quince cultivars (Cydonia oblonga Mill.). International Journal of Horticultural Science 6(3):103-108. https://doi.org/10.31421/IJHS/6/1/76
Booth A, Klimenko S (2001). Capability for the self-pollination of the quince’s sorts of the selection of the National Botanical Garden in Kyiv. Proceedings of 9th International Conference of Horticulture, Lednice, Czech Republic, September 3-6, pp 31-36.
Certal AC, Almeida RB, Bošković R, Oliveira MM, Feijó JA (2002). Structural and molecular analysis of self-incompatibility in almond (Prunus dulcis). Sexual Plant Reproduction 15:13-20. https://doi.org/10.1007/s00497-002-0138-4
Čolić S, Zec G, Fotirić M, Rahović D, Janković Z (2010). Evaluation of self-(in)compatibility in the almond (Prunus amygdalus Batsch) genotype population from the Slankamen Hill, Serbia. Archives of Biological Sciences 62(4):973-979. https://doi.org/10.2298/ABS1004973C
Dicenta F, Ortega E, Cánovas JA, Egea J (2002). Self-pollination vs. cross pollination in almond, pollen tube growth, fruit set and fruit characteristics. Plant Breeding 121:163-167. https://doi.org/10.1046/j.1439-0523.2002.00689.x
Đordević M, Cerović R, Radičević S, Nikolić D (2014). Incompatible pollen tubes in the plum style and their impact on fertilisation success. Genetika 46(2):411-418. https://doi.org/10.2298/GENSR1402411D
Evans NA, Hoyne PA (1982). A fluorochrome from aniline blue, structure, synthesis and fluorescence properties. Australian Journal of Chemistry 35(12):2571-2575. https://doi.org/10.1071/CH9822571
Frankling-Tong N, Franklin FCH (2003). Gametophytic self-incompatibility inhibits pollen tube growth using different mechanisms. Trends in Plant Science 8(12):598-605. https://doi.org/10.1016/j.tplants.2003.10.008
Halász J, Hegedûs A (2006). A critical evaluation of methods used for S-genotyping, from trees to DNA level. International Journal of Horticultural Science 12(2):19-29. https://doi.org/10.31421/IJHS/12/2/631
Jacquemart AL, Michotte-Van Der AАA, Raspé O (2006). Compatibility and pollinator efficiency tests on Pyrus communis L. cv. ‘Conference’. Journal of Horticultural Science and Biotechnology 81(5):827-830. https://doi.org/10.1080/14620316.2006.11512145
Jia HJ, He FJ, Xiong CZ, Zhu FR, Okamoto G (2008). Influences of cross pollination on pollen tube growth and fruit set in Zuili plums (Prunus salicina). Journal of Integrative Plant Biology 50(2):203-209. https://doi.org/10.1111/j.1744-7909.2007.00382.x
Kaufmane E, Rumpunen K (2002). Pollination, pollen tube growth and fertilization in Chaenomeles japonica (Japanese quince). Scientia Horticulturae 94:257-271. https://doi.org/10.1016/S0304-4238(01)00371-5
Kho O, Baër J (1971). Fluorescence microscopy in botanical research. Zeiss Information 76:54-57.
Lansari A, Iezzoni A (1990). A preliminary analysis of self-incompatibility in sour cherry. HortScience 25(12):1636-1638. https://doi.org/10.21273/HORTSCI.25.12.1636
Lopez M, Alonso JM, Martinez-Gomez P, Sociasi Company R, Gradziel TM, BatlleI (2001). Biological and molecular assessment of self-incompatibility in almond. Nucis 10:8-11.
Milatović D, Nikolić D (2007). Analysis of self-(in)compatibility in apricot cultivars using fluorescence microscopy. Journal of Horticultural Science and Biotechnology 82(2):170-174. https://doi.org/10.1080/14620316.2007.11512215
Milatović D, Nikolić D, Fotirić-Akšić M, Radović A (2013a). Testing of self-(in)compatibility in apricot cultivars using fluorescence microscopy. Acta Scientiarum Polonorum, Hortorum Cultus 12(6):103-113.
Milatović D, Nikolić D, Krška B (2013b). Testing of self (in)compatibility in apricot cultivars from European breeding programmes. Horticultural Science 40(2):65-71. https://doi.org/10.17221/219/2012-HORTSCI
Milatović D, Nikolić D, Rakonjac V, Fotirić-Akšić M (2010). Cross-(in)compatibility in apricot cultivars. Journal of Horticultural Science and Biotechnology 85:394-398. https://doi.org/10.1080/14620316.2010.11512686
Nettancourt D de (2001). Incompatibility and incongruity in wild and cultivated plants. 2nd Edition. Springer-Verlag, Berlin Heidelberg New York.
Newbigin E, Anderson MA, Clarke AE (1993). Gametophytic self-incompatibility systems. The Plant Cell 5:1315-1324. https://doi.org/10.1105/tpc.5.10.1315
Nikolić D, Milatović D (2010). Examining self-compatibility in plum (Prunus domestica L.) by fluorescence microscopy. Genetika 42(2):387-396. https://doi.org/10.2298/GENSR1002387N
Ontivero M, Radice S, Giordani E, Bellini E (2006). Effects of different pollination treatments in genotypes of Prunus salicina Lindl. International Journal of Horticultural Science 12(2):141-146. https://doi.org/10.31421/IJHS/12/2/647
Radović A, Cerović R, Milatović D, Nikolić D (2020). Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.). Spanish Journal of Agricultural Research 18(2):e0702. https://doi.org/10.5424/sjar/2020182-15551
Rae AL, Harris PJ, Bacic A, Clarke AE (1985). Composition of the cell walls of Nicotiana alata Link et Otto pollen tubes. Planta 166(1):128-133. https://doi.org/10.1007/BF00397395
Sanzol J, Herrero М (2002). Identification of self-incompatibility alleles in pear cultivars (Pyrus communis L.). Euphytica 128:325-331. https://doi.org/10.1023/A:1021213905461
Sanzol J, Herrero М (2007). Self-incompatibility and self-fruitfulness in pear cv. Agua de Aranjuez. Journal of the American Society for Horticultural Science 132(2):166-171. https://doi.org/10.21273/JASHS.132.2.166
Szabó T, Nyéki J, Soltész M, Szabó Z, Tóth T (1999). Time of flowering and fertilisation of quince varieties. International Journal of Horticultural Science 5(1-2):9-15. https://doi.org/10.31421/IJHS/5/1-2/13
Tobutt KR, Bošković R, Cerović R, Sonneveld T, Ružić Đ (2004). Identification of incompatibility alleles in the tetraploid species sour cherry. Theoretical and Applied Genetics 108:775-785. https://doi.org/10.1007/s00122-003-1511-x
Vuletin Selak G, Perica S, Goreta Ban S, Radunic M, Poljak M (2011). Reproductive success after self-pollination and cross-pollination of olive cultivars in Croatia. HortScience 46(2):186-191. https://doi.org/10.21273/HORTSCI.46.2.186
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Copyright (c) 2023 Aleksandar RADOVIĆ, Dragan NIKOLIĆ, Dragan MILATOVIĆ, Ivana RADOVIĆ, Dejan ZEJAK, Velibor SPALEVIC, Branislav DUDIĆ
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