Pollen morphology and anatomy of Rubus L. (Rosaceae) in Thailand

Authors

  • Kasan HANCHANA Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150 (TH)
  • Surapon SAENSOUK Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Application Research Center, Mahasarakham 44150 (TH)
  • Piyaporn SAENSOUK Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Application Research Center, Mahasarakham 44150 (TH)

DOI:

https://doi.org/10.15835/nbha51113070

Keywords:

anatomy, palynology, Rosaceae, Rubus, Thailand

Abstract

Pollen morphology and anatomy were investigated in 19 species of the genus Rubus L. from Thailand by observation and analysis under a light microscope and a scanning electron microscope. The pollen grains are monads, with radial symmetry, circular shaped in polar view and isopolar. The sizes of the pollen grains are small and medium. Considering the species studied, the majority have medium sized pollen grains. There were different shapes detected viz., oblate, suboblate, prolate spheroidal, oblate spheroidal and prolate. The pollen grains have various apertures: tricolporate, tricolporate-tetracolporate, tricolpate and tricolpate-tetracolpate. There were five forms of exine sculpturing as follows: rugulate-perforate, striate-perforate, microverrucate-perforate, microverrucate and perforate. In the genus Rubus it is not possible to use the morphology of the pollen for the identification to species level. Eleven species had the morphology of the pollen studied for the first time. The anatomy of the leaf, petiole and stem were observed by transverse section. The anatomy can be used for the identification to species level. The results indicate that the anatomical characteristics used for the identification key are shape of leaf margin, shape of adaxial surface of midrib, pericyclic fibers, idioblast cell, shape of petiole, starch grain, number of vascular bundles, shape of stem and types of trichomes. In this study, the anatomy of 19 species was reported for the first time. The numerical analysis based on the pollen morphology and anatomy data did not support classification of the genus Rubus L.

References

Alice LA, Campbell CS (1999). Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany 86(1):81-97. https://doi.org/10.2307/2656957

Chwil M, Kostryco M (2020). Histochemical assays of secretory trichomes and the structure and content of mineral nutrients in Rubus idaeus L. leaves. Protoplasma 257(1):119-139. https://link.springer.com/article/10.1007/s00709-019-01426-7

Chwil M, Kostryco M (2020). Histochemical assays of secretory trichomes and the structure and content of mineral nutrients in Rubus idaeus L. leaves. Protoplasma 257(1):119-139. https://doi.org/10.1007/s00709-019-01426-7

Erdtman G (1966). Pollen morphology and plant taxonomy: Angiosperms. Noble Offset Printers, New York.

Fell KR, Rowson JM (1956). Anatomical studies in the genus Rubus. Part I. The anatomy of the leaf of Rubus idoeus L. Journal of Pharmacy and Pharmacology 8(1):334-345. https://doi.org/10.1111/j.2042-7158.1956.tb12165.x

Fell KR, Rowson JM (1957). Anatomical studies in the genus Rubus. Part II. The anatomy of the leaf of Rubus fruticosus L. Journal of Pharmacy and Pharmacology 9(1):293-311. https://doi.org/10.1111/j.2042-7158.1957.tb12282.x

Fell KR, Rowson JM (1960). Anatomical studies in the genus Rubus. Part III. The anatomy of the leaf of Rubus loganobaccus. Journal of Pharmacy and Pharmacology 12(1):473-487. https://doi.org/10.1111/j.2042-7158.1960.tb12695.x

Fell KR, Rowson JM (1961). Anatomical studies in the genus Rubus. Part IV. the anatomical variations in the leaves of cultivated varieties of R. idaeus L. and R. loganobaccus L.H. Bailey, and of certain species of bramble. Journal of Pharmacy and Pharmacology 13(1):83-92. https://doi.org/10.1111/j.2042-7158.1961.tb11792.x

Focke WO (1910). Ruborum monographiae generis Rubi prodromus. Bibliotheca Botanica 17(72):1-120. http://dx.doi.org/10.5962/bhl.title.15533

Focke WO (1911). Ruborum monographiae generis Rubi prodromus. Bibliotheca Botanica 17(72):121-223. http://dx.doi.org/10.5962/bhl.title.15533

Focke WO (1914). Ruborum monographiae generis Rubi prodromus. Bibliotheca Botanica 19(83):1-274. http://dx.doi.org/10.5962/bhl.title.15533

Ghosh A, Saha I (2017). Pollen morphological study of some selected Indian taxa of Rosaceae. Indian Journal of Applied and Pure Biology 32(2):121-130. http://biology-journal.org/journal/volume32/issue64/ijapb32-2-3.html

Hutchinson J (1964). The genera of flowering plants. Vol 1. Dicotyledons. Clarendon Press, Oxford.

Kalkman C (1993). Rosaceae. Flora Malesiana-Series 1. Spermatophyta 11(2):227-351. https://repository.naturalis.nl/pub/532506

Kasalkheh R, Jorjani E, Sabouri H, Habibi M, Sattarian A (2019). Leaflet epidermal studies and taxonomic significance of trichomes in the Rubus subgenus Rubus (Rosaceae) in Iran. Phytologia Balcanica International Journal of Balkan Flora and Vegetation 25(1):53-61. http://www.bio.bas.bg/~phytolbalcan/PDF/25_1/contents.html

Kubitzki K (2004). The families and genera of vascular plants. Vol. 6. Flowering plants. Dicotyledons: Celastrales, Oxalidales, Rosales, Cornales and Ericales. Springer, Berlin.

Kurz S (1877). Forest Flora of British Burma Vol. 1. superintendent of government printing, Calcutta. https://www.biodiversitylibrary.org/item/112239#page/1/mode/1up

Lechowicz K, Bocianowski J, Wrońska-Pilarek D (2021). Pollen morphology and variability of species from the genus Rubus L. (Rosaceae) alien and invasive in Poland. Webbia 76(1):109-121. https://doi.org/10.36253/jopt-10355

Lechowicz K, Wrońska-Pilarek D, Bocianowski J, Maliński T (2020). Pollen morphology of polish species from the genus Rubus L. (Rosaceae) and its systematic importance. Plos One 15(5):1-24. https://doi.org/10.1371/journal.pone.0221607

Linnaeus C (1753). Species plantarum tome I. Holmiae, Stockholm, 491-502.

https://www.biodiversitylibrary.org/item/13829#page/1/mode/1up

Lu LD, Boufford DE, Alexander C, Bartholomew B, Brach AR, Endress PK, … Zhang Z (2003). Flora of China 9. Science Press, Beijing. http://www.efloras.org/volume_page.aspx?volume_id=2009&flora_id=2

Metcalfe CR, Chalck L (1950). Anatomy of the dicotyledons Vol 1. Clarendon Press, Oxford.

Morgan DR, Soltis DE, Robertson KR (1994). Systematic and evolutionary implications of rbcL sequence variation in Rosaceae. American Journal of Botany 81(7):890-903. https://doi.org/10.1002/j.1537-2197.1994.tb15570.x

Potter D, Eriksson T, Evans RC, Oh S, Smedmark JEE, Morgan DR, … Campbell CS (2007). Phylogeny and classification of Rosaceae. Plant Systematics and Evolution 226(1):5-43. https://doi.org/10.1007/s00606-007-0539-9

Punt W, Hoen PP, Blackmore S, Nilsson S, Le Thomas A (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143:1-81. https://doi.org/10.1016/j.revpalbo.2006.06.008

Schulze-Menz GK (1964). Engler’s Syllabus der Pflanzenfamilien II. Gebruder Boerntraeger, Berlin.

Takhtajan A (1997). Diversity and classification of flowering plants. Columbia University Press, New York.

Thammathaworn A (1995). Handbook for permanent slides of plant tissue by paraffin method. Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.

Thuan NV (1970). Rosaceae. In: Smitinand TK Larsen (Ed). Flora of Thailand. Vol. 2, Part 1. ASRCT Press, Bangkok, pp 31-74.

Thuan, NV (1968). Rosaceae. In: Aubréville A (Ed). Flora du Cambodge du Laos et du Vietnam. Vol. 7 (Rubus). Museum national D’Histoire Naturelle, Paris, pp 1-78.

Tomaszewski D, Zieliński J, Gawlak M (2014). Foliar indumentum in central-European Rubus species (Rosaceae) and its contribution to the systematics of the group. Nordic Journal of Botany 32(1):1-10. https://doi.org/10.1111/j.1756-1051.2013.00116.x

Tomlik-Wyremblewska A (1995). Pollen morphology of the genus Rubus L. I. Introductory studies on the European representatives of the subgenus Rubus L. Acta Societatis Botanicorum Poloniae 64(2):187-203. https://doi.org/10.5586/asbp.1995.027

Tomlik-Wyremblewska A, Van Der Ham RWJM, Kosiński P (2004). Pollen morphology of genus Rubus L. Part III. Studies on the Malesian species of subgenera Chamaebatus L. and Idaeobatus L. Acta Societatis Botanicorum Poloniae 73(3):207-227. https://doi.org/10.5586/asbp.2004.028

Tomlik-Wyremblewska A, Zieliński J, Guzicka M (2010). Morphology and anatomy of blackberry pyrenes (Rubus L., Rosaceae) elementary studies of the european representatives of the genus Rubus L. Flora-Morphology, Distribution, Functional Ecology of Plants 205(6):370-375. https://doi.org/10.1016/j.flora.2009.12.006

Upadhyaya MK, Furness NH (1998). Primocane morphology and leaf surface characteristics of greenhouse-grown red raspberry cultivars. Horticultural Science 33(2):330-332.

Vidal JE (1968). Rosaceae. In: Aubréville A (Ed). Flora du Cambodge du Laos et du Vietnam. Vol. 6. Museum national D’Histoire Naturelle, Paris, pp 1-178.

Wada S, Reed BM (2008). Morphological analysis of Rubus seed. Acta Horticulturae 782:67-74. https://doi.org/10.17660/ActaHortic.2008.782.5

Wang YF, Chen SH (2001). Pollen flora of yuenyang lake nature preserve, Taiwan (II). Taiwania 46(2):167-191. https://doi.org/10.6165/tai.2001.46(2).167

Wrońska-Pilarek D, Jagodziński AM, Maliński T (2012). Morphological studies of pollen grains of the polish endemic species of the genus Rubus (Rosaceae). Biologia 67(1):87-96. https://doi.org/10.2478/s11756-011-0141-z

Xiong XH, Zhou XM, Li M, Xu B, Deng HN, Yu Q, Gao XF (2019). Pollen morphology in Rubus (Rosaceae) and its taxonomic implications. Plant Systematics and Evolution 305(8):705-716. https://doi.org/10.1007/s00606-019-01600-7

Published

2023-02-20

How to Cite

HANCHANA, K., SAENSOUK, S., & SAENSOUK, P. (2023). Pollen morphology and anatomy of Rubus L. (Rosaceae) in Thailand. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), 13070. https://doi.org/10.15835/nbha51113070

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha51113070

Most read articles by the same author(s)