Phytoremediation potential of native plants: Biomonitoring approach in contaminated soils
DOI:
https://doi.org/10.15835/nbha51213063Keywords:
environmental polluants, phytoextraction, phytostabilisation, soil contaminationAbstract
Phytoremediation has been used to remediate contaminated site. This study aims to investigate the potential of the selected native plants to accumulate and tolerate chemical pollutants. Two sites were selected in Southern Tunisia lands (Gabès), i) Gannouch located near the chemical industrial complex and ii) Zerkineis around 35 kilometres far from the industrial activities. Soil and plants were sampled and analysed for cadmium (Cd), lead (Pb), copper (Cu), zinc (Zn) and fluorine (F) concentrations. The native plants were Bassia indica and Chenopodium album, with no obvious morphological toxic symptoms. Results indicated that the upper soil layers were alkaline and salt affected with low organic matter content. The highest levels of fluoride and heavy metal were found in Ghannouch. The contents of Cd, Cu, Pb, Zn and F in the roots, stem and leaves of the native plants exceed the normal ranges and the phytotoxic level. Bioconcentration factor (BCF), translocation factor (TF), and biological accumulation factor (BAF) were determined for each element. Based on these factors the selected plants were classified as excluders, indicators, and accumulators for each element. The native plants, studied herein, seems to have significant potential for phytoremediation, thus they may be used as biomonitors in contaminated soils. Bassia indica identified as Cd, Cu and Pb accumulator, could be an excellent candidate for phytoextraction and phyto stabilization because of its F excluder performance.
References
Abdal N, Abbas G, Asad SA, Ghfar AA, Shah GM, Rizwan M, ... Shahbaz M (2023). Salinity mitigates cadmium-induced phytotoxicity in quinoa (Chenopodium quinoa Willd.) by limiting the Cd uptake and improved responses to oxidative stress: Implications for phytoremediation. Environmental Geochemistry and Health 45(1):171-185. https://doi.org/10.1007/s10653-021-01082-y
Agoubi B, Gzam M (2016). Adverse effects of phosphate industry on the environment and groundwater geochemistry in the Ghannouch field, Southeastern Tunisia. American Journal of Geophysics, Geochemistry and Geosystems 2(5):51-63.
Ahmad I, Gul I, Irum S, Manzoor M, Arshad M (2022). Accumulation of heavy metals in wild plants collected from the industrial sites—Potential for phytoremediation. International Journal of Environmental Science and Technology 1-12. https://doi.org/10.1007/s13762-022-04340-3
Alaya MB, Saidi S, Zemni T, Zargouni F (2014). Suitability assessment of deep groundwater for drinking and irrigation use in the Djeffara aquifers (Northern Gabes, south-eastern Tunisia). Environmental Earth Sciences 71(8):3387-3421. https://doi.org/10.1007/s12665-013-2729-9
Awa SH, Hadibarata T (2020). Removal of heavy metals in contaminated soil by phytoremediation mechanism: A Review. Water Air & Soil Pollution 231(2):47. https://doi.org/10.1007/s11270-020-4426-0
Aydi S, Sassi Aydi S, Rahmani R, Bouaziz F, Souchard JP, Merah O, Abdelly C (2023). Date-palm compost as soilless substrate improves plant growth, photosynthesis, yield and phytochemical quality of greenhouse melon (Cucumis melo L.). Agronomy 13:212. https://doi.org/10.3390/agronomy13010212.
Aziz I, Mujeeb A (2022). Halophytes for phytoremediation of hazardous metal (loid)s: A terse review on metal tolerance, bio-indication and hyperaccumulation. Journal of Hazardous Materials 424:127309. https://doi.org/10.1016/j.jhazmat.2021.127309
Bashir S, Hussain Q, Jun ZHU, Qingling FU, Houben D, Hongqing HU (2020). Efficiency of KOH-modified rice straw-derived biochar for reducing cadmium mobility, bioaccessibility and bioavailability risk index in red soil. Pedosphere 30(6):874-882. https://doi.org/10.1016/S1002-0160(20)60043-1
Ben Amor A, Bagues M, Elloumi N, Chaira N, Rahmani R, Nagaz K (2021). Suitability of four main Mediterranean tree crops for their growth in peri-urban agriculture and restoration (Gabes, Tunisia). Environmental Science and Pollution Research 28(15):19034-19045. https://doi.org/10.1007/s11356-020-12102-2
Ben Amor A, Rahmani R, Bennani L, Ben Yahia L, Ben Atia Zrouga K, Chaira N, Nagaz K (2023). Investigation of phenolic compounds potential to reduce dust pollution of pomegranate trees. International Journal of Phytoremediation 25(4):430-440. https://doi.org/10.1080/15226514.2022.2089090
Ben Chabchoubi I, Bouguerra S, Ksibi M, Hentati O (2021). Health risk assessment of heavy metals exposure via consumption of crops grown in phosphogypsum-contaminated soils. Environmental Geochemistry and Health 43(5):1953-1981. https://doi.org/10.1007/s10653-020-00777-y
Borjigin S, Ashimura Y, Yoshioka T, Mizoguchi T (2009). Determination of fluoride using ion-selective electrodes in the presence of aluminum. Analytical Sciences 25(12):1437-1443. https://doi.org/10.2116/analsci.25.1437
Boukhris A, Laffont-Schwob I, Folzer H, Rabier J, Mezghani I, Salducci M-D, Tatoni T, Chaieb M (2018). Tolerance strategies of two Mediterranean native xerophytes under fluoride pollution in Tunisia. Environmental Science and Pollution Research 25(34):34753-34764. https://doi.org/10.1007/s11356-018-3431-y
Buszewski B, Jastrzębska A, Kowalkowski T, Górna-Binkul A (2000). Monitoring of selected heavy metals uptake by plants and soils in the area of Toruń, Poland. Poland Journal of Environmental Studies 9(6):511-515.
Chandra P, Dhuli P, Verma P, Singh A, Choudhary M, Prajapat K, Rai AK, Yadav RK (2020). Culturable microbial diversity in the rhizosphere of different biotypes under variable salinity. Tropical Ecology 61(3):291-300. https://doi.org/10.1007/s42965-020-00089-3
Chen X, Kumari D, Cao CJ, Plaza G, Achal V (2019). A review on remediation technologies for nickel-contaminated soil. Human and Ecological Risk Assessment: An International Journal 26(3):571-585. https://doi.org/10.1080/10807039.2018.1539639
Choudhary S, Rani M, Devika OS, Patra A, Singh RK, Prasad SK (2019). Impact of fluoride on agriculture: A review on it’s sources, toxicity in plants and mitigation strategies. International Journal of Chemical Studies 7(2):1675-1680.
Claus J, Bohmann A, Chavarría-Krauser A (2013). Zinc uptake and radial transport in roots of Arabidopsis thaliana: a modelling approach to understand accumulation. Annals of Botany 112(2):369-380. https://doi.org/10.1093/aob/mcs263.
Cronin SJ, Manoharan V, Hedley MJ, Loganathan P (2000). Fluoride: A review of its fate, bioavailability, and risks of fluorosis in grazed-pasture systems in New Zealand. New Zealand Journal of Agricultural Research 43(3):295-321. https://doi.org/10.1080/00288233.2000.9513430
Dahri N, Atoui A, Ellouze M, Abida H (2018). Assessment of streambed sediment contamination by heavy metals: The case of the Gabes Catchment, South-eastern Tunisia. Journal of African Earth Sciences 140:29-41. https://doi.org/10.1016/j.jafrearsci.2017.12.033
Egendorf SP, Groffman P, Moore G, Cheng Z (2020). The limits of lead (Pb) phytoextraction and possibilities of phytostabilization in contaminated soil: A critical review. International Journal of Phytoremediation 22(9):916-930. https://doi.org/10.1080/15226514.2020.1774501
Eid EM, Shaltout KH (2016). Bioaccumulation and translocation of heavy metals by nine native plant species grown at a sewage sludge dump site. International Journal of Phytoremediation 18(11):1075-1085. https://doi.org/10.1080/15226514.2016.1183578
Elloumi N, Belhaj D, Mseddi S, Zouari M, Ben Abdallah F, Woodward S, Kallel M (2017). Response of Nerium oleander to phosphogypsum amendment and its potential use for phytoremediation. Ecological engineering 99:164-171. https://doi.org/10.1016/j.ecoleng.2016.11.053
Enya O, Heaney N, Iniama G, Lin C (2020). Effects of heavy metals on organic matter decomposition in inundated soils: Microcosm experiment and field examination. Science of the total environment 724:138223. https://doi:10.1016/j.scitotenv.2020.138223
Fong SS, Kanakaraju D, Ling SC (2006). Evaluation of the acid digestion method with different solvent combination for the determination of Iron, Zinc and Lead in canned sardines. Malaysian Journal of Chemistry 8 (1):10-15.
García MG, Borgnino L (2015). Fluoride in the context of the environment. Fluorine: Chemistry, Analysis, Function and Effects 3-21. https://doi.org/10.1039/9781782628507-00003
Hashem A, Abd_Allah EF, Alqarawi AA, Malik JA, Wirth S, Egamberdieva D (2019). Role of calcium in AMF-mediated alleviation of the adverse impacts of cadmium stress in Bassia indica. Saudi Journal of Biological Sciences 26(4):828-838. https://doi.org/10.1016/j.sjbs.2016.11.003
He Z, Shentu J, Yang X, Baligar VC, Zhang T, Stoffella PJ (2015). Heavy metal contamination of soils: sources, indicators and assessment. Journal of Environmental Indicators 9:17-18.
Hechmi S, Hamdi H, Mokni-Tlili S, Ghorbel M, Khelil MN, Zoghlami IR, Benzarti S, Jellali S, Hassen A, Jedidi N (2020). Impact of urban sewage sludge on soil physico-chemical properties and phytotoxicity as influenced by soil texture and reuse conditions. Journal of Environmental Quality 49(4):973-986. https://doi.org/10.1002/jeq2.20093.
Henchi B (2019). Pollution and the deteriorating quality of life in Tunisia. The North African Environment at Risk 207-216.
Hong B-D, Joo R-N, Lee K-S, Lee D-S, Rhie J-H, Min S, Song S-G, Chung D-Y (2016). Fluoride in soil and plant. Korean Journal of Agricultural Science 43(4):522-536. http://dx.doi.org/10.7744/kjoas.20160054
Hossain MB, Masum, Z, Rahman, MS, Yu J, Noman MA, Jolly YN, Begum BA, Paray BA, Arai T (2022). Heavy metal accumulation and phytoremediation potentiality of some selected mangrove species from the world’s largest mangrove forest. Biology 11(8):1144. https://doi.org/10.3390/biology11081144
Jha SK, Mishra VK, Sharma DK, Damodaran T (2011). Fluoride in the environment and its metabolism in humans. Reviews of Environmental Contamination and Toxicology 211:121-142. https://doi.org/10.1007/978-1-4419-8011-3_4
Jiang Z, Nie K, Yu L, Arinzechi C, Zhao F, Liao Q, Yang W (2023). Synchronous stabilization of As, Cd, and Pb in soil by sustained-release of iron-phosphate. Science of The Total Environment 161369. https://doi.org/10.1016/j.scitotenv.2022.161369
Kabata-Pendias A, Szteke B (2015). Trace elements in abiotic and biotic environments. Taylor & Francis. https://library.oapen.org/handle/20.500.12657/41690.
Kenawy IMM, Hafez MAH, Akl MA, Lashein RR (2000). Determination by AAS of some trace heavy metal ions in some natural and biological samples after their preconcentration using newly chemically modified chloromethylated polystyrene-PAN ion-exchanger. Analytical Sciences 16(5):493-500. https://doi.org/10.2116/analsci.16.493
Ketata M, Hamzaoui F, Gueddari M, Bouhlila R, Ribeiro L (2011). Hydrochemical and statistical study of groundwaters in Gabes-south deep aquifer (south-eastern Tunisia). Physics and Chemistry of the Earth, Parts A/B/C 36(5-6):187-196. https://doi.org/10.1016/j.pce.2010.02.006
Krämer U (2010). Metal hyperaccumulation in plants. Annual Review of Plant Biology 61(1):517-534. https://doi.org/10.1146/annurev-arplant-042809-112156
Kumar S, Prasad S, Yadav KK, Shrivastava M, Gupta N, Nagar S, & Malav LC (2019). Hazardous heavy metals contamination of vegetables and food chain: Role of sustainable remediation approaches-A review. Environmental Research 179:108792. https://doi.org/10.1016/j.envres.2019.108792
Lamas F, Irigaray C, Oteo C, Chacon J (2005). Selection of the most appropriate method to determine the carbonate content for engineering purposes with particular regard to marls. Engineering Geology 81(1):32-41. https://doi.org/10.1016/j.enggeo.2005.07.005
Lam EJ, Keith BF, Bech J, Alvarez FA, Zetola V, Pereira LH, Montofré ÍL (2022). Characteristic curve modeling of plant species behavior in soils with heavy metals. Environmental Geochemistry and Health 1-14. https://doi.org/10.1007/s10653-022-01342-5.
Li C, Zhou K, Qin W, Tian C, Qi M, Yan X, Han W (2019). A review on heavy metals contamination in soil: Effects, sources, and remediation techniques. Soil and Sediment Contamination: An International Journal 28(4):380-394. https://doi.org/10.1080/15320383.2019.1592108
Lutts S, Lefèvre I (2015). How can we take advantage of halophyte properties to cope with heavy metal toxicity in salt-affected areas? Annals of Botany 115(3):509-528. https://doi.org/10.1093/aob/mcu264
Luo W, Gao X, Zhang X (2018). Geochemical processes controlling the groundwater chemistry and fluoride contamination in the Yuncheng Basin, China—An area with complex hydrogeochemical conditions. PloS One 13(7):e0199082. https://doi.org/10.1371/journal.pone.0199082
Madhav S, Ahamad A, Singh AK, Kushawaha J, Chauhan JS, Sharma S, Singh P (2020). Water pollutants: Sources and impact on the environment and human health. Sensors in Water Pollutants Monitoring: Role of Material 43-62. https://doi.org/10.1007/978-981-15-0671-0_4
Mahamood M, Khan FR, Zahir F, Javed M. Alhewairini SS (2023). Bagarius bagarius, and Eichhornia crassipes are suitable bioindicators of heavy metal pollution, toxicity, and risk assessment. Scientific Reports 13(1):1824. https://doi.org/10.1038/s41598-023-28313-9
Malik RN, Husain SZ, Nazir I (2010). Heavy metal contamination and accumulation in soil and wild plant species from industrial area of Islamabad. Pakistan. Pakistan Journal of Botany 42(1):291-301.
Maurya J, Seema PSN, Ghosh AK (2020). Evaluation of ground water quality and health risk assessment due to nitrate and fluoride in the Middle Indo-Gangetic plains of India. Human and Ecological Risk Assessment: An International Journal 27(5):1349-1365. https://doi.org/10.1080/10807039.2020.1844559
McGrath SP, Shen ZG, Zhao FJ (1997). Heavy metal uptake and chemical changes in the rhizosphere of Thlaspicaerulescens and Thlaspiochroleucum grown in contaminated soils. Plant and Soil 188(1):153-159. https://doi.org/10.1023/A:1004248123948
Mezghani I, Elloumi N, Ben Abdallah F, Chaieb M, Boukhris M (2005). Fluoride accumulation by vegetation in the vicinity of a phosphate fertilizer plant in Tunisia. Fluoride 38(1):69-75.
Midhat L, OuazzaniN, Hejjaj A, Ouhammou A, Mandi L (2019). Accumulation of heavy metals in metallophytes from three mining sites (Southern Centre Morocco) and evaluation of their phytoremediation potential. Ecotoxicology and Environmental Safety 169:150-160. https://doi.org/10.1016/j.ecoenv.2018.11.009
Nasfi Z, Busch H, Kehraus S, Linares-Otoya L, König GM, Schäberle TF, Bachoual R (2018). Soil bacteria isolated from Tunisian arid areas show promising antimicrobial activities against gram-negatives. Frontiers in microbiology 9:2742. https://doi.org/10.3389/fmicb.2018.02742.
Nazir A, Malik RN, Ajaib M, Khan N, Siddiqui MF (2011). Hyperaccumulators of heavy metals of industrial areas of Islamabad and Rawalpindi. Pakistan Journal of Botany 43(4):1925-1933.
Nouri J, Khorasani N, Lorestani B, Karami M, Hassani AH, Yousefi N (2009). Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environmental Earth Sciences 59(2):315-323. https://doi.org/10.1007/s12665-009-0028-2
Parvez M (2022). Heavy metals levels and associated health risk assessment of Euphorbia granulata Forssk. Environmental Science and Pollution Research 29(2):1998-2008. https://doi.org/10.1007/s11356-021-15569-9
Peng C, Tong H, Shen C, Sun L, Yuan P, He M, Shi J (2020). Bioavailability and translocation of metal oxide nanoparticles in the soil-rice plant system. Science of the Total Environment, 713: 136662. https://doi.org/10.1016/j.scitotenv.2020.136662
Pouresmaieli M, Ataei M, Forouzandeh P, Azizollahi P, Mahmoudifard M (2022). Recent progress on sustainable phytoremediation of heavy metals from soil. Journal of Environmental Chemical Engineering 10(5):108482. https://doi.org/10.1016/j.jece.2022.108482
Printz B, Lutts S, Hausman JF, Sergeant K (2016). Copper trafficking in plants and its implication on cell wall dynamics. Frontiers in Plant Science 7:601. https://doi.org/10.3389/fpls.2016.00601
Raj A, Mandal J, Kumari PB (2020). Organo-metallic complex and its implications. Journal of Pharmacognosy and Phytochemistry 9(2):491-500. https://doi.org/10.22271/phyto.2020.v9.i2i.10904
Reeves RD (2006). Hyperaccumulation of trace elements by plants. In Phytoremediation of metal-contaminated soils 25-52. https://doi.org/10.1007/1-4020-4688-X2
Saha P, Shinde O, Sarkar S (2017). Phytoremediation of industrial mines wastewater using water hyacinth. International Journal of Phytoremediation 19(1):87-96. https://doi.org/10.1080/15226514.2016.1216078
Sharma I (2020). Bioremediation techniques for polluted environment: Concept, advantages, limitations, and prospects. In: Trace Metals in the Environment-New Approaches and Recent Advances. IntechOpen. https://doi.org/10.5772/intechopen.90453
Sharma JK, Kumar N, Singh NP, Santal AR (2023). Phytoremediation technologies and its mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment. Frontiers in Plant Science 14:78. https://doi.org/10.3389/fpls.2023.1076876
Sheoran V, Sheoran AS, Poonia P (2009). Phytomining: A review. Minerals Engineering 22(12):1007-1019. https://doi.org/10.1016/j.mineng.2009.04.001
Silva GJ, Ferraz-Almeida R (2023). Scrubber filter in the phosphate fertilizer factory reduces fluorine emission and accumulation in corn. Air 1 (1):69-79. https://doi.org/10.3390/air1010005
Siyar R, Doulati Ardejani F, Norouzi P, Maghsoudy S, Yavarzadeh M, Taherdangkoo R, Butscher C (2022). Phytoremediation potential of native hyperaccumulator plants growing on heavy metal-contaminated soil of Khatunabad copper smelter and refinery, Iran. Water 14 (22):3597. https://doi.org/10.3390/w14223597
Skuza L, Szućko-Kociuba I, Filip E, Bożek I (2022). Natural molecular mechanisms of plant hyperaccumulation and hypertolerance towards heavy metals. International Journal of Molecular Sciences 23(16):9335. https://doi.org/10.3390/ijms23169335
Smolders E (2001). Cadmium uptake by plants. International Journal of Occupational Medicine and Environmental Health 14(2):177-183.
Svensson DN, Messing I, Barron J (2022). An investigation in laser diffraction soil particle size distribution analysis to obtain compatible results with sieve and pipette method. Soil and Tillage Research 223:105450. https://doi.org/10.1016/j.still.2022.105450
Tuffour HO, Iby MB, Adjei-Gyapong T, Khalid AA, Abubakar A, Melenya C, Kpotor P (2014). Soil organic carbon: Relating the walkley-black wet oxidation method to loss on ignition and clay content. International Journal of Scientific Research in Knowledge 2:249-256. http://dx.doi.org/10.12983/ijsrk-2014-p0249-0256
Ugulu I, Khan ZI, Rehman S, Ahmad K, Nawaz K, Munir M, Bashir H (2022). Heavy metal accumulation in goosefoot (Chenopodium album L.) irrigated with wastewater. Pakistan Journal of Analytical & Environmental Chemistry 23(2):225-236. http://dx.doi.org/10.21743/pjaec/2022.12.05
Vardhan KH, Kumar PS, Panda RC (2019). A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives. Journal of Molecular Liquids 290:111197. https://doi.org/10.1016/j.molliq.2019.111197
Venkateswaran P, Vellaichamy S, Palanivelu K (2007). Speciation of heavy metals in electroplating industry sludge and wastewater residue using inductively coupled plasma. International Journal of Environmental Science & Technology 4:497-504. https://doi.org/10.1007/BF03325986
Wong JWC, Li KL, Zhou LX, Selvam A (2007). The sorption of Cd and Zn by different soils in the presence of dissolved organic matter from sludge. Geoderma 137(3-4):310-317. https://doi.org/10.1016/j.geoderma.2006.08.026
World Health Organization News and activities (1996). Bulletin of the World Health Organization 74(1):109-115.
Xu Y, Seshadri B, Bolan N, Sarkar B, Ok YS, Zhang W, Rumpel C, Sparks D, Farrell M, Hall T (2019). Microbial functional diversity and carbon use feedback in soils as affected by heavy metals. Environment International 125:478-488. https://doi.org/10.1016/j.envint.2019.01.071
Yadav N, Rani K, Yadav SS, Yadav DK, Yadav VK, Yadav N (2018). Soil and water pollution with fluoride, geochemistry, food safety issues and reclamation—A review. International Journal of Current Microbiology and Applied Sciences 7:1147-1162. https://doi.org/10.20546/ijcmas.2018.705.140
Yan A, Wang Y, Tan SN, Mohd Yusof ML, Ghosh S, Chen Z (2020). Phytoremediation: A promising approach for revegetation of heavy metal-polluted land. Frontiers in Plant Science 11:359. https://doi.org/10.3389/fpls.2020.00359
Yan W, Hamid N, Deng S, Jia P-P, Pei D-S (2020). Individual and combined toxicogenetic effects of microplastics and heavy metals (Cd, Pb, and Zn) perturb gut microbiota homeostasis and gonadal development in marine medaka (Oryzias melastigma). Journal of hazardous materials 397:122795. https://doi.org/10.1016/j.jhazmat.2020.122795.
Yang Z, Yang F, Liu JL, Wu HT, Yang H, Shi Y, ... Chen KM (2022). Heavy metal transporters: Functional mechanisms, regulation, and application in phytoremediation. Science of The Total Environment 809:151099. https://doi.org/10.1016/j.scitotenv.2021.151099
Zhao Y-L, Zhu L-L, Sun Y, Zhou D-Q (2015). Determination of fluoride in Antarctic krill (Euphausia superba) using ion chromatography and its pretreatments selection. Czech Journal of Food Sciences 33(1):77-82. https://doi.org/10.17221/498/2013-CJFS
Zhu G, Xiao H, Guo Q, Song B, Zheng G, Zhang Z, Zhao J, Okoli CP (2018). Heavy metal contents and enrichment characteristics of dominant plants in wasteland of the downstream of a lead-zinc mining area in Guangxi, Southwest China. Ecotoxicology and Environmental Safety 151:266-271. https://doi.org/10.1016/j.ecoenv.2018.01.011
Zulfiqar U, Ayub A, Hussain S, Waraich EA, El-Esawi MA, Ishfaq M, Ahmad M, Ali N, Maqsood MF (2021). Cadmium toxicity in plants: Recent progress on morpho-physiological effects and remediation strategies. Journal of Soil Science and Plant Nutrition 1-58. https://doi.org/10.1007/s42729-021-00645-3.
Zwolak A, Sarzyńska M, Szpyrka E, Stawarczyk K (2019). Sources of soil pollution by heavy metals and their accumulation in vegetables: A review. Water, Air, & Soil Pollution 230:1-9. https://doi.org/10.1007/s11270-019-4221-y
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Copyright (c) 2023 Samir AYDI, Sameh SASSI AYDI, Ghada BEN SALAH, Amani DEKHIL, Rami RAHMANI, Abdelhakim BOUAJILA, Niveen A. MOHAMED, Chedly ABDELLY
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