In vitro regeneration from adult node explants of Juniperus oxycedrus
DOI:
https://doi.org/10.15835/nbha51113062Keywords:
Cupressaceae, cytokinins, explant decontamination, olive medium, 2iPAbstract
In the present study the in vitro propagation from adult native plants of Juniperus oxycedrus was investigated. The effect of seasonal explant collection (spring, summer, winter), in varying media and cytokinin type were examined. The decontamination process of explants taken in the summer was more efficient than that of the other seasons (57.3% of the explants survived). In the spring a low performance in shoot formation took place both on hormone free Murashige and Skoog (MS) media and Rugini Olive Medium (OM) or each containing 6-benzyladenine (BA) at 1.0 mg L-1 by which shoots did not elongate more than 0.5 cm. In the summer OM was used, and a higher in vitro performance was observed on medium containing 0.1 mg L-1 6-(γ,γ-Dimethylallylamino)purine (2iP); the shoot formation percentage was 72.0%, and respectively 3.1 shoots, 1.4 cm in length, were formed. In the winter, the shoot formation percentage was 92.0-100.0%, and respectively 3.9 shoots of 0.4 cm length were formed on medium containing 1.0 mg L-1 2iP. The multiplication phase on cytokinin-containing media led to a satisfactory proliferation rate in terms of shoot formation percentage and shoot number. The higher shoot number (3.3) was recorded on media containing 2iP at 1.0 mg L-1, while the shoot length was longer (2.0) cm, on hormone-free media. Initiation of roots was not observed during the rooting phase on hormone-free half-strength OM, or supplemented with 1.0 and 2.0 mg L-1 IBA. Spontaneous rooting at a low percentage (10.0%) was observed on OM media supplemented with 0.1 mg L-1 2iP and successful acclimatisation took place on a 1:1 (v/v) ratio of peat to perlite substrate.
References
Adams RP (2004). Junipers of the World: the genus Juniperus. Trafford Publishing Co., Bloomington, Indiana.
Ahani H, Jalilvand H, Hosseini Nasr SM, Soltani Kouhbanani H, Ghazi MR, Mohammadzadeh, H (2013). Reproduction of juniper (Juniperus polycarpos) in Khorasan Razavi, Iran. Forest Science and Practice 15:231-237. https://doi.org/10.1007/s11632-013-0307-6
Al-Ramamneh EAD, Daradkeh N, Rababah T, Pacurar D., Al-Qudah M (2017). Effects of explant, media and growth regulators on 'in vitro' regeneration and antioxidant activity of Juniperus phoenicea. Australian Journal of Crop Science 11(7):828-837. https://doi.org/10.21475/ajcs.17.11.07.pne479
Arista M, Ortiz PL, Talavera S (2001). Reproductive cycles of two allopatric subspecies of Juniperus oxycedrus (Cupressaceae). Flora 196(2):114-120. https://doi.org/10.1016/S0367-2530(17)30026-9
Baravardi H, Ranjbar GA, Kamali S, Abadi F (2014). Investigation of the effects of growth regulators on callus induction in Juniperus excelsa L. Bulletin of Environment, Pharmacology and Life Sciences 4(1):80-84.
Berhe D, Negash L (1998). Asexual propagation of Juniperus procera from Ethiopia: a contribution to the conservation of African pencil cedar. Forest Ecology and Management 112(1-2):179-190. https://doi.org/10.1016/S0378-1127(98)00327-2
Bertsouklis K, Paraskevopoulou AT, Zarkadoula N (2020). In vitro propagation of Juniperus phoenicea L. Acta Horticulturae 1298:331-334. https://doi.org/10.17660/ActaHortic.2020.1298.45
Brito, G (2000). Micropropagação de duas espécies autóctones da Ilha de Porto Santo (Olea europaea L. ssp. maderensis Lowe e Juniperus phoenicea L.) e estudo da resposta de rebentos in vitro a stress osmótico. M.Sc. thesis (Aveiro: University of Aveiro)
Ceballos L, Ruiz J (1971). Arboles y arbustos de la España peninsular. Instituto Forestal de Investigaciones Agrarias y Escuela Técnica Superior de Ingenieros de Montes, Madrid, Spain, pp 512.
Chambers RM, Meyerson LA, Saltonstall K (1999). Expansion of Phragmites australis into tidal wetlands of North America. Aquatic Botany 64(3-4):261-273. https://doi.org/10.1016/S0304-3770(99)00055-8
de Foresta H, Somarriba E, Temu A, Boulanger D, Feuilly H, Gauthier M (2013). Towards the assessment of trees outside forests. Forest Resources Assessment Working Paper 183. FAO, pp 335
Farjon A (2010). A Handbook of the world's conifers. Koninklijke Brill, Leiden
Farjon A (2013). Juniperus oxycedrus. The IUCN red list of threatened species 2013: e.T42243A2965838. Retrieved 2022 December 24 from https://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T42243A2965838.en
Ferradous A, Alifriqui M, Hafidi M, Duponnois R (2013). Essais de régénération artificielle du Genévrier thurifere (Juniperus thurifera L.). Ecologia Mediterranea 39(1):115-120
Garcia D (2001). Effects of seed dispersal on Juniperus communis recruitment on a Mediterranean mountain. Journal of Vegetation Science 12:839-848. https://doi.org/10.2307/3236872
Gomez MP, Segura J (1994). Factors controlling adventitious bud induction and plant regeneration in mature Juniperus oxycedrus leaves cultured in vitro. In vitro Cellular and Developmental Biology-Plant 30:210-218. https://doi.org/10.1007/BF02823034
Gómez MP, Segura J (1995). Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree physiology 15(9):625-628. https://doi.org/10.1093/treephys/15.9.625
Hazubska-Przybył T (2019). Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review. Forests 10(11):1028. https://doi.org/10.3390/f10111028
Ilashi I (1986). Juniper (Juniperus polycarpos C. Koch) In: Bajaj YPS (Ed). Biotechnology in Agriculture and Forestry. Springer, Berlin, Heidelberg, Germany pp 321-325.
Kartsonas E, Papafotiou M (2007). Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tissue Organ Culture 90:111-11. https://doi.org/10.1007/s11240-007-9232-5
Khater N, Benbouza H (2019). Preservation of Juniperus thurifera L.: a rare endangered species in Algeria through in vitro regeneration. Journal of Forestry Research 30:77-86. https://doi.org/10.1007/s11676-018-0628-3
Kocer ZA, Gozen AG, Onde S, Kaya Z (2011). Indirect organogenesis from bud explants of Juniperus communis L: effects of genotype, gender, sampling time and growth regulator. Dendrobiology 66:33-40
Korakis G (2015). Forest Botany [Undergraduate textbook]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/742
Kumar R, Sharma K, Agrawal V (2005). In vitro clonal propagation of Holarrhena antidysenterica (L.) Wall. through nodal explants from mature trees. In vitro Cellular and Developmental Biology-Plant 41:137-144. https://doi.org/10.1079/IVP2004624
Loureiro J, Capelo A, Brito G, Rodriguez, E, Silva S, Pinto G, Santos C (2007). Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry. Biologia Plantarum 51(1):7-14 https://doi.org/10.1007/s10535-007-0003-2
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Negussie A (1997). In vitro induction of multiple buds in tissue culture of Juniperus excelsa. Forest Ecology and Management 98:115-123. https://doi.org/10.1016/S0378-1127(97)00034-0
Oliveira P, Barriga J, Cavaleiro C, Peixe A, Potes AZ (2003). Sustained in vitro root development obtained in Pinuspinea L. inoculated with ectomycorrhizal fungi. Forestry 76:579-587. https://doi.org/10.1093/forestry/76.5.579
Ortiz PL, Arista M, Talavera S (1998). Low reproductive success in two subspecies of Juniperus oxycedrus L. International Journal of Plant Sciences 159(5):843-847. https://doi.org/10.1086/297605
Rugini E (1984). In vitro propagation of some olive (Olea europaea L. var. sativa) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae (Amsterdam) 24 (2):123-134 https://doi.org/10.1016/0304-4238(84)90143-2
Schenk RV and Hildebrandt AC (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany 50:199-204. https://doi.org/10.1139/b72-026
Torchik V (2010). Evaluation of an assortment of ornamental forms of conifers in Central Botanical Garden of Belarus National Academy of Science. Acta Horticulturae 885:375-381. https://doi.org/10.17660/ActaHortic.2010.885.53
Unlu M, Vardar-Unlu G, Vural N, Dönmez E, Cakmak O (2008). Composition and antimicrobial activity of Juniperus excelsa essential oil. Chemistry of Natural Compounds, 44: 129-131. https://doi.org/10.1007/s10600-008-0040-x
Wang Y, Yao R (2017). Plantlet regeneration of adult Pinus massoniana Lamb. trees using explants collected in March and thidiazuron in culture medium. Journal of Forestry Research 28:1169-1175. https://doi.org/10.1007/s11676-017-0412-9

Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Konstantinos BERTSOUKLIS, Angeliki T. PARASKEVOPOULOU, Evdoxia PETRAKI

This work is licensed under a Creative Commons Attribution 4.0 International License.
License:
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.