Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens
DOI:
https://doi.org/10.15835/nbha51213031Keywords:
antagonistic, Bacillus, fungal phytopathogen, fruit Juice, plant based media, proliferationAbstract
Plant-based media have recently been of interest as potential natural media for microbial culture. This study was conducted to apply inexpensive and available fruits to culture Bacillus antagonizing three fungal phytopathogens including Phytophthora capsici GTC 2.6.1, Rhizoctonia solani GTC 2.7.1, and Sclerotium rolfsii GTC 2.9.1. The results showed that the juice media of dragon fruit, Cavendish banana, watermelon, pineapple, and MT1 seedless guava could all be used to culture two screened antagonistic Bacillus strains including B08 and B18. Surveys on the media from watermelon juice indicated that juice concentration (based on mass of fruit pulp), initial pH, concentration of traditional medium added to the juice medium had different effects on the growth of two strains of Bacillus sp. B08 and B18. These preliminary results demonstrated the potential application of the juice in biomass production of antagonistic Bacillus strains. This approach is certainly safe for the environment and has the potential to improve fruit consumption and reduce the cost of microbial fertilizer production, so it needs more research attention.
References
Adeleke O, Oguntuga OA (2003). Nutrient analyses and investigation into the potential of watermelon flesh-juice for wine production. Bioscience Research Communications 15(2):169-175.
Alwakeel SS, Al-Humaidi EAH (2008). Microbial growth and chemical analysis of mineral contents in bottled fruit juices and drinks in Riyadh, Saudi Arabia. Research Journal of Microbiology 3:319-325. https://doi.org/10.3923/jm.2008.319.325
Andrade CP, Menezes KS, Da Silva TF, Martins LA, Cartonilho M De M, Kirsch L (2020). Cultivation of Pleurotus eryngii in tropical tubers and roots found in the amazon region. Brazilian Journal of Development 6(6):34131-34146. https://doi.org/10.34117/bjdv6n6-094
Aneja KR, Dhiman R, Aggarwal NK, Kumar V, Kaur M (2014). Microbes associated with freshly prepared juices of citrus and carrots. International Journal of Food Science Volume 2014:408085. http://dx.doi.org/10.1155/2014/408085
Anjhana VR, Sasikala SL (2017). Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens. International Journal of Advanced Research in Biological Sciences 4(12):15-26. http://dx.doi.org/10.22192/ijarbs.2017.04.12.003
Anjum MZ, Adnan M, Ali SM, Bilal HM, Javaid H (2019). Antifungal potential of biocontrol agents against Phytophthora capsici causing chili fruit rot. Agricultural Research & Technology 22(4):00156-00159. https://doi.org/10.19080/ARTOAJ.2019.22.556209
Beauregarda PB, Chaib Y, Vlamakisa H, Losickb R, Kolter R (2013). Bacillus subtilis biofilm induction by plant polysaccharides. The Proceedings of the National Academy of Sciences (PNAS) 110(17):E1621-E1630. https://doi.org/10.1073/pnas.1218984110
Cahya VA, Hanim C, Yusiati LM, Bachruddin Z, Erwanto Y (2019). Growth optimization of Bacillus subtilis 11A isolated from Indonesian native chicken (Gallus domesticus) for bacteriocin production. IOP Conference Series: Earth and Environmental Science 387(2019):012013. https://doi.org/10.1088/1755-1315/387/1/012013
Cichońska P, Ziarno M (2022). Legumes and legume-based beverages fermented with lactic acid bacteria as a potential carrier of probiotics and prebiotics. Microorganisms 10(1):91. https://doi.org/10.3390/microorganisms10010091
Cruz CH da S, Santos JB dos, Santos FP dos, Silva GMM, Cruz EFN da, Ramos GL de PA, Nascimento J dos S (2020). Texturized soy protein as an alternative low-cost media for bacteria cultivation. Bacterial Empire 3(4):74-76. http://dx.doi.org/10.36547/be.2020.3.4.74-76
Devidas PC, Pandit BH, Vitthalrao PS (2014). Evaluation of different culture media for improvement in bioinsecticides production by indigenous Bacillus thuringiensis and their application against larvae of Aedes aegypti. The Scientific World Journal 2014:273030. http://dx.doi.org/10.1155/2014/273030
Elsawey H, Patz S, Nemr RA, Sarhan MS, Hamza MA, Youssef HH, … Hegazi NA (2020). Plant broth- (not bovine-) based culture media provide the most compatible vegan nutrition for in vitro culturing and in situ probing of plant microbiota. Diversity 12:418. https://doi.org/10.3390/d12110418
Evelyn, Utami SP, Chairul (2022). Effect of temperature and soluble solid on Bacillus subtilis and Bacillus licheniformis spore inactivation and quality degradation of pineapple juice. Food Science and Technology International 28(4):285-296. https://doi.org/10.1177/10820132211019143
Fish WW, Bruton BD, Russo VM (2009). Watermelon juice: A promising feedstock supplement, diluent, and nitrogen supplement for ethanol biofuel production. Biotechnology Biofuels 2:18. https://doi.org/10.1186/1754-6834-2-18
García-Sánchez M, Robledo-Ortiz J, Jiménez-Palomar I, González-Reynoso O, González-García Y (2019). Production of bacterial cellulose by Komagataeibacter xylinus using mango waste as alternative culture medium. Revista Mexicana De Ingeniería Química 19(2):851-865. https://doi.org/10.24275/rmiq/Bio743
Ghasemi S, Ahmadzadeh M (2013). Optimisation of a cost-effective culture medium for the large-scale production of Bacillus subtilis UTB96. Archives of Phytopathology and Plant Protection 46(13):1552-1563. https://doi.org/10.1080/03235408.2013.771469
Harper AR, Dobson RCJ, Morris VK, Moggré GJ (2022). Fermentation of plant-based dairy alternatives by lactic acid bacteria. Microbial Biotechnology 15(5):1404-1421. https://doi.org/10.1111/1751-7915.14008
Hassan LE, Sirat HM, Yagi SM, Koko WS, Abdelwahab SI (2011). In vitro antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides (wild melon). Journal of Medicinal Plants Research 5:1338-1344.
Heenan CN, Adams MC, Hosken RW, Fleet GH (2002). Growth medium for culturing probiotic bacteria for applications in vegetarian food products. Lwt - Food Science and Technology 35:171-176. https://doi.org/10.1006/fstl.2001.0833
Ibrahim SE, Mohamed HF, Shehata HS, Salah El Din RA (2020a). Perineal plant extract as a culture medium for production of plant growth regulators by molecularly identified rhizospheric microorganisms. Global Advanced Research Journal of Agricultural Science 9(2):027-039.
Ibrahim SE, Shehata HS, Mohamed HF, Salah El Din, RA (2020b). Overproduction of some plant growth promoters by rhizospheric microorganisms on natural medium. Global Journal of Science Frontier Research 20(1):6-21.
Jadhav P, Sonne M, Kadam A, Patil S, Dahigaonkar K, Oberoi JK (2018). Formulation of cost effective alternative bacterial culture media using fruit and vegetables waste. International Journal of Current Research and Review 10(2):6-15. https://doi.org/10.7324/IJCRR.2018.1022
Kavuthodi B, Malavika NJ, Antony A, Sebastian D (2015). Biotechnological prospective of watermelon rind for economical production of exo-pectinase using Bacillus subtilis BKDS1 by submerged fermentation. In: Bijesh K, Malavika NJ, Antony A, Sebastian D (Eds). Proceedings of International Conference on Biodiversity & Evaluation: Perspectives and Paradigm shifts. Sree Sankara College, Kalady, India pp 223-226.
Kumari P, Bishnoi SK, Chandra S (2021). Assessment of antibiosis potential of Bacillus sp. against the soil-borne fungal pathogen Sclerotium rolfsii Sacc. (Athelia rolfsii (Curzi) Tu & Kimbrough). Egyptian Journal of Biological Pest Control 31:17. https://doi.org/10.1186/s41938-020-00350-w
Kusumaningrum KS, Andayani S, Yuniarti A (2020). Effect of different C:N ratio media on the spore production of Bacillus firmus. International Journal of Scientific & Technology Research 9(02):1640-1643.
Lani MN, Mohdmangsor NH, Sharifudin SA, Abdullah WZW, Mohdisa NS, Jamzuri MNS, Mohdmaidin N (2022). Chemical and microbiological analysis of fermented probiotic watermelon juice. Food Research 6(3):324-334. https://doi.org/10.26656/fr.2017.6(3).402
Magarelli RA, Trupo M, Ambrico A, Larocca V, Martino M, Palazzo S, … Bevivino A (2022). Designing a waste-based culture medium for the production of plant growth promoting microorganisms based on cladodes juice from Opuntia ficus-indica. Pruning. Fermentation 8:225. https://doi.org/10.3390/fermentation8050225
Margani R, Hadiwiyono, Widadi S (2018). Utilizing Bacillus to inhibit the growth and infection by sheath blight pathogen, Rhizoctonia solani in rice. IOP Conference Series: Earth and Environmental Science 142(2018):012070. https://doi.org/10.1088/1755-1315/142/1/012070
Maryati Y, Susilowati A (2018). Fermentation of pitaya (Hylocereus polyrhizus) juice by LactobaciLlus acidophilus in metabolism of sugars for cholesterol removal. AIP Conference Proceedings 2026. Indonedia. https://doi.org/10.1063/1.5065024
Mohammed BB, Shatti ZO, Jasim EI, Dari WA, Alfraji MDN (2020). Local culture medium from the legumes mixture as a novel media for the growth and stimulation of prodigiosin pigment which production from Serratia marcescens that isolated environmentally. Plant Archives 20(1):991-1000.
Mojica-Marín V, Luna-Olvera HA, Sandoval-Coronado CF, Pereyra-Alférez B, Morales-Ramos LH, Hernández-Luna CE, Alvarado-Gomez OG (2008). Antagonistic activity of selected strains of Bacillus thuringiensis against Rhizoctonia solani of chili pepper. African Journal of Biotechnology 7(9):1271-1276.
Moon JH, Won SJ, Maung CEH, Choi JH, Choi SI, Ajuna HB, Ahn YS (2021). Bacillus velezensis CE 100 inhibits root rot diseases (Phytophthora spp.) and promotes growth of Japanese cypress (Chamaecyparis obtusa Endlicher) seedlings. Microorganisms 9(4):821. https://doi.org/10.3390/microorganisms9040821
Mukisa IM, Birungi SW (2018). Proximate composition, acceptability and stability of probiotic dairy yoghurt containing cooking banana/matooke puree and Lactobacillus rhamnosus yoba. Journal of Microbiology, Biotechnology and Food Science 7(4):343–347. https://doi.org/10.15414/jmbfs.2018.7.4.343-347
Naga Sivudu S, Umamahesh K, Reddy OVS (2014). A comparative study on probiotication of mixed watermelon and tomato juice by using probiotic strains of Lactobacilli. International Journal of Current Microbiology and Applied Sciences 3(11):977-984.
Nawangsih EN, Kusnawan PH, Djamaludin M (2021). The growth curve and total colonies number of Lactobacillus spp on the pink guava (Psidium guajava Linnaeus) juice medium. Proceedings of the 12th Annual Scientific Meeting. Medical Faculty, Universitas Jenderal Achmad Yani, Indonesia, Atlantis Press. https://doi.org/10.2991/ahsr.k.210723.019
Nguyen BT, Bujna E, Fekete N, Tran ATM, Rezessy-Szabo JM, Prasad R, Nguyen QD (2019). Probiotic beverage from pineapple juice fermented with Lactobacillus and Bifidobacterium strains. Frontiers in Nutrition 6:54. https://doi.org/10.3389/fnut.2019.00054
Nurainy F, Rizal S, Suharyono S, Susanti E (2022). Characteristic of red guava juice probiotic beverages with the addition of cinnamon and sappan wood extract. Jurnal Penelitian Pertanian Terapan 22(2):195-204. https://doi.org/10.25181/jppt.v22i2.2149
Ozcelik B, Yavuz M (2016). Watermelon Juice. In: Shahidi F, Alasalvar C (Eds). Handbook of Functional Beverages and Human Health. CRC Press, Taylor & Francis Group, London pp 553-562.
Pathak M, Martirosyan D (2012). Optimization of an effective growth medium for culturing probiotic bacteria for applications in strict vegetarian food products. Functional Foods in Health and Disease 2(10):369-378. https://doi.org/10.31989/ffhd.v2i10.75
Rajkumar K, Naik MK, Amaresh YS, Chennappa G (2018). In vitro screening of Bacillus subtilis isolates against Sclerotium rolfsii cause for collar rot of chilli. International Journal of Current Microbiology and Applied Sciences 7(7):2687-2692. https://doi.org/10.20546/ijcmas.2018.707.315
Reddy VV, Vemulapati BM, Srinivasulu R, Golamari SR (2017). Development of watermelon agar medium and muskmelon agar medium. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(1):209-213.
Rios-Velasco C, Caro-Cisneros JN, Berlanga-Reyes DI, Ruiz-Cisneros MF, Ornelas‐Paz JD, Salas-Marina MÁ, Villalobos-Pérez EM, Guerrero-Prieto VM (2016). Identification and antagonistic activity in vitro of Bacillus spp. and Trichoderma spp. isolates againsts common phytopathogenic fungi. Mexican Journal of Phytopathology 34(1):84-99. https://doi.org/10.18781/R.MEX.FIT.1507-1
Santos FPD, Magalhães DCMMD, Nascimento JDS, Ramos GLDPA (2022). Use of products of vegetable origin and waste from hortofruticulture for alternative culture media. Food Science and Technology 42:e00621. https://doi.org/10.1590/fst.00621
Sarhan MS, Mourad EF, Nemr RA, Abdelfadeel MR, Daanaa HSA, Youssef HH, … Hegazi NA (2020). An inoculum-dependent culturing strategy (IDC) for the cultivation of environmental microbiomes and the isolation of novel endophytic Actinobacteria. The Journal of Antibiotics 73:66-71. https://doi.org/10.1038/s41429-019-0226-4
Satapute PP, Olekar HS, Shetti AA, Kulkarni AG, Hiremath GB, Patagundi BI, … Kaliwal BB (2012). Isolation and characterization of nitrogen fixing Bacillus subtilis strain as-4 from agricultural soil. International Journal of Recent Scientific Research 3(9):762-765.
Saxena AK, Kumar M, Chakdar H, Anuroopa N, Bagyaraj DJ (2020). Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology 128(6):1583-1594. https://doi.org/10.1111/jam.14506
Shareef AS (2019). Formulation of alternative culture media from natural plant protein sources for cultivation of different bacteria and fungi. Zanco Journal of Pure and Applied Sciences 31(4):61-69. https://doi.org/10.21271/zjpas.31.4.7
Shojaaddini M, Moharramipour S, Khodabandeh M, Talebi AA (2010). Development of a cost effective medium for production of Bacillus thuringiensis bioinsecticide using food barley. Journal of Plant Protection Research 50(1):9-14. https://doi.org/10.2478/v10045-010-0002-8
Sidorova TM, Asaturova AM, Homyak AI, Zhevnova NA, Shternshis MV, Shternshis MV, Omashevich NS (2020), Optimization of laboratory cultivation conditions for the synthesis of antifungal metabolites by Bacillus subtilis strains. Saudi Journal of Biological Sciences 27:1879-1885. https://doi.org/10.1016/j.sjbs.2020.05.002
Supono, Hutabarat J, Prayitno SB, Darmanto YS (2013). The effect of different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc inoculated with bacterium Bacillus cereus. Journal of Coastal Development 16(2):114-120.
Suryadi BF, Masyari M, Tresnani G (2019), Utilization of lablab bean (Lablab purpureus) and palm sugar (arengapinnata) as natural medium to grow Mataram indigenous isolate of entomopathogenic Bacillus thuringiensis for controlling Aedes aegypti larvae. IOP Conference Series: Materials Science and Engineering 546:022031. https://doi.org/10.1088/1757-899X/546/2/022031
Tekiner N, Kotan R, Tozlu E, Dadaşoğlu F (2019). Determination of some biological control agents against Alternaria fruit rot in quince. Alinteri Journal of Agriculture Sciences 34(1):25-31.
Uppala SS, Zhou XG, Liu BH, Wu ML (2019). Plant-based culture media for improved growth and sporulation of Cercospora janseana. Plant Disease 103(3):504-508. https://doi.org/10.1094/PDIS-05-18-0814-RE
Willey JM, Sandman KM, Wood DH (2023). Prescott’s microbiology. McGraw Hill, New York.
Youssef HH, Hamza MA, Fayez M, Mourad EF, Saleh MY, Sarhan MS, … Hegazi NA (2016). Plant-based culture media: Efficiently support culturing rhizobacteria and correctly mirror their in situ diversity. Journal of Advanced Research 7(2):305-316. https://doi.org/10.1016/j.jare.2015.07.005
Yuniarti A, Arifin NB, Fakhri M, Hariati AM (2019). Effect of C:N ratio on the spore production of Bacillus sp. indigenous shrimp pond. The 1st International Conference on Fisheries and Marine Science. IOP Conference Series: Earth and Environmental Science 236:012029. https://doi.org/10.1088/1755-1315/236/1/012029
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