In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub

Konstantinos BERTSOUKLIS; Kostandin NAKSI; Paraskevi-Evangelia ARETAKI

doi:10.15835/nbha51112992

Authors

Konstantinos BERTSOUKLIS Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
Kostandin NAKSI Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
Paraskevi-Evangelia ARETAKI Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)

DOI:

https://doi.org/10.15835/nbha51112992

Keywords:

climatisation, in vitro rooting, micropropagation, new ornamental, tissue culture

Abstract

Τhis research was carried out at the prospect of introducing new ornamental species in the floriculture industry concerning the use of valuable plants. A leguminous species native to Australia was the object of the present study. Seeds of Senna artemisioides established in a private botanical garden were chosen as the starting material for both germination and in vitro studies. Hot water treatment at 90 °C, for 10 min was applied to interrupt the seed-coat dormancy. Incubation was conducted in vitro in Petri dishes, at seven different temperatures ranging from of 5.0 to 35.0 °C, involving three separate treatments of incubation at 5.0 °C, for 0, 30 and 60 days. The seeds germinated at 25 °C (56.0%). Node explants derived from produced seedlings were cultured in vitro onto Murashige and Skoog media (MS), hormone free (Hf) or containing 6-Benzyladenine (BA). The establishment of initial cultures was successful being higher on MS supplemented with BA at the range 0.1-1.0 mg L^-1media. The multiplication phase followed on MS media containing the same range of a total of four cytokinins widely used in in vitro experimentation, i.e BA, Kinetin (KIN), N6-Isopentenyladenine (2IP) and Zeatin (ZEA). 1-Naphthaleneacetic acid (NAA) was tested in conjunction with the higher concentration of the four cytokinins. MS media supplemented with BA at 0.5 mg L^-1led to a satisfactory proliferation rate; 2.4 shoots/explant were formed. Rooting phase had moderate efficiency, being higher for media with 4.0 mg L^-1 IBA. Acclimatisation was successful for all rooted plantlets, promising an effective method for exploitation of this new ornamental species of high value.

References

Agrawal V, Sardar PR (2006). In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli. Biologia Plantarum 50(1):118-122. https://doi.org/10.1007/s10535-005-0084-8

Ahmad A, Ahmad N, Anis M, Alatar AA, Abdel-Salam EM, Qahtan AA, Faisal M (2021). Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings. Plant Cell, Tissue and Organ Culture (PCTOC) 144(2):449-462. https://doi.org/10.1007/s11240-020-01969-1

Ahmed MR, Ahmad N, Anis M, Aref, IM (2013). Efficient plantlet regeneration from cotyledonary node cultures of Cassia alata L. (Ringworm Bush): stimulatory effect of thidiazuron. Journal of Horticultural Science and Biotechnology 88:141-146.

Al-Helal AA, Al-Farraj, M M, El-Desoki RA, Al-Habashi I (1989). Germination response of Cassia senna L. seeds to sodium salts and temperature. Journal of the University of Kuwait(science). Kuwait 16(2):281-288.

Allan SM, Adkins SW, Preston CA, Bellairs SM (2004). Improved germination of the Australian natives: Hibbertia commutata, Hibbertia amplexicaulis (Dilleniaceae), Chameascilla corymbosa (Liliaceae) and Leucopogon nutans (Epacridaceae). Australian Journal of Botany 52:345-351. https://doi.org/10.1071/BT03097

Amoo SO, Finnie JF, Van Staden J (2011). The role of meta-topolins in alleviating micropropagation problems. Plant Growth Regulation 63:197-206. https://doi.org/10.1007/s10725-010-9504-7

Auld TD, O’Connell MA (1991). Predicting patterns of post-fire germination in 35 eastern Australian Fabaceae. Australian Journal of Ecology 16:53-70. https://doi.org/10.1111/j.1442-9993.1991.tb01481.x

Baskin CC, Baskin JM (1998). Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego, CA, pp 666.

Baskin CC, Baskin JM (2003). Classification, biogeograhpy, and phylogenetic relationships of seed dormancy. In: Smith R, Dickie J, Linington S, Pritchard H, Probert R (Eds). Seed Conservation: Turning Science into Practice. The Royal Botanic Gardens, Kew: London, pp 518-544.

Baskin J, Nan X, Baskin C (1998). A comparative study of seed dormancy and germination in an annual and a perennial species of Senna (Fabaceae). Seed Science Research 8(4):501-512. https://doi.org/10.1017/S0960258500004475

Baskin JM, Baskin CC (2004) A classification system for seed dormancy. Seed Science Research 14:1-16. https://doi.org/101079/SSR2003150

Baskin JM, Baskin CC, Li X (2000) Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biology 15:139-152. https://doi.org/10.1046/j.1442-1984.2000.00034.x

Baskin JM, Baskin CC (1973). Plant population differences in dormancy and germination characteristics of seeds: heredity or environment? The American Midland Naturalist 90(2):493-498. https://doi.org/10.2307/2424478

Bertsouklis K, Panagaki K-P (2022). In vitro germination and propagation of Dyckia brevifolia, an ornamental and endangered Bromeliad. Horticulturae 8(5):390. https://doi.org/10.3390/horticulturae8050390

Bunn E, Turner SR, Dixon KW (2011). Biotechnology for saving rare and threatened flora in a biodiversity hotspot. In vitro Cellular and Developmental Biology-Plant 47:188-200. https://doi.org/10.1007/s11627-011-9340-0

Cabahug RAM, Nam SY, Lim KB, Jeon JK, Hwang YJ (2018). Propagation techniques for ornamental succulents. Korean Flower Association 26:90-101.

Carra A, Catalano C, Badalamenti O, Carimi F, Pasta S, Motisi A, (...) Garfì G (2019). Overcoming sexual sterility in conservation of endangered species: the prominent role of biotechnology in the multiplication of Zelkova sicula (Ulmaceae), a relict tree at the brink of extinction. Plant Cell, Tissue and Organ Culture (PCTOC) 137(1):139-148. https://doi.org/10.1007/s11240-019-01558-x

Carruggio F, Onofri A, Impelluso C, Giusso del Galdo G, Scopece G, Cristaudo A (2020). Seed dormancy breaking and germination in Bituminaria basaltica and B. bituminosa (Fabaceae). Plants 9(9):1110. https://doi.org/10.3390/plants9091110

Curir P, Damiano C, Cosmi T (1986). In vitro propagation of Genista monosperma. HortScience 21:859.

De Klerk GJ (2002). Rooting of microcuttings: theory and practice. In Vitro Cellular and Developmental Biolology-Plant 38:415-422. https://doi.org/10.1079/IVP20 02335

Donohue K (2009). Completing the cycle: Maternal effects as the missing link in plant life histories. Philosophical Transactions of the Royal Society B Biological Sciences 364:1059-1074. https://doi.org/10.1098/rstb.2008.0291

Donohue K, De Casas RR, Burghardt LT, Kovach K, Willis CG (2010). Germination, post germination adaptation, and species Ecological Ranges. Annual Review of Ecology, Evolution and Systematics 41:293-319. https://www.annualreviews.org/doi/10.1146/annurev-ecolsys-102209-144715

Ellis RH, Roberts EH (1981). The quantification of aging and survival in orthodox seeds. Seed Science and Technology 9(2):373-409.

Feng HH, Al-Khayri JM, Gbur EE (1994). Micropropagation of Acacia mearnsii. In Vitro Cellular and Developmental Biology-Plant 30:70-74.

Fett-Neto AG, Fett JP, Aquila MEA, Ferreira AG (2000). In vitro propagation of Senna alata. Planta Medica 66(02):195-196.

Finch-savage WE, Bassel GW (2016). Seed vigour and crop establishment: Extending performance beyond adaptation. Journal of Experimental Botany 67:567-591. https://doi.org/10.1093/jxb/erv490

Gbadamosi AE, Hassan KO (2013). In vitro propagation of Senna alata (linn) on WPM and MS media with varying PGR. International Journal of Development and Sustainability 2:1997-2007.

Generoso AL, Carvalho VS, Walter R, Campbell G, Araújo LS, Santana JGS, Cunha M (2019). Mature-embryo culture in the cryopreservation of passion fruit (Passifora edulis Sims) seeds. Scientia Horticulturae 256:108638. https://doi.org/10.1016/j.scienta.2019.108638

Holman JE, Playford J (2000). Molecular and morphological variation in the Senna artemisioides complex. Australian Journal of Botany 48(5):569-579. https://doi.org/10.1071/BT98032

International Seed Testing Association (1999). International rules for seed testing. Seed Science and Technology 27(suppl):333.

IPNI (2022). International plant names index. The Royal Botanic Gardens, Kew, Harvard University Herbaria & Libraries and Australian National Botanic Gardens. Retrieved 25 October 2022 from Published on the Internet http://www.ipni.org

Kwok AB, Eldridge DJ (2016). The influence of shrub species and fine-scale plant density on arthropods in a semiarid shrubland. The Rangeland Journal 38(4):381-389. https://doi.org/10.1071/RJ15019

Luckow M (1996). The cultivated species of Cassia, Senna, and Chamaecrista (Leguminosae). Baileya 23:195-241.

Maguire JD (1962). Speed of germination-Aid in selection and evaluation for seedling emergence and vigor. Crop Science 2:176-177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x

Marazzi B, Sanderson MJ (2010). Large-scale patterns of diversification in the widespread legume genus Senna and the evolutionary role of extrafloral nectaries. Evolution 12:3570-3592. https://doi.org/10.1111/j.1558-5646.2010.01086.x.

McCown BH, Lloyd G (1981). Woody plant medium (WPM)-a revised mineral formulation for micro-culture of woody plant species. HortScience 16:453.

Msanga HP (1998). Seed germination of indigenous trees in Tanzania: Including Notes on Seed Processing, Storage and Plant Uses, Canadian Forest Service, Northern Forestry Centre (OCoLC) 41504804, Edmonton, Canada.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Norsworthy JK, Oliveira MJ (2005). Coffee senna (Cassia occidentalis) germination and emergence is affected by environmental factors and seeding depth. Weed Science 53(5):657-662.

Papafotiou M, Bertsouklis KF, Trigka M (2013). Micropropagation of Arbutus unedo, A. andrachne, and their natural hybrid, A. × andrachnoides from seedling explants. Journal of Horticultural Science and Biotechnology 6:768-775. https://doi.org/10.1080/14620316.2013.11513037

Papafotiou M, Martini AN, Bertsouklis KF, Vlachou G, Kanellou E, Stylias EG, Kalantzis A, … Dariotis E (2022). A first approach for evaluation, breeding and promotion of native to Greece sage species for use as landscape plants. Acta Horticulturae 1345:359-366. https://doi.org/10.17660/ActaHortic.2022.1345.48

Parveen S, Shahzad A (2014). Factors affecting in vitro plant regeneration from cotyledonary node explant of Senna sophera (L.) Roxb.-A highly medicinal legume. African Journal of Biotechnology 13(3). https://doi.org/10.5897/AJB2013.13126

Penfield S, MacGregor DR (2017). Effects of environmental variation during seed production on seed dormancy and germination. Journal of Experimental Botany 68(4):819-825. https://doi.org/10.1093/jxb/erw436

Pipinis E, Hatzilazarou S, Kostas S, Bourgou S, Megdiche-Ksouri W, Ghrabi-Gammar Z, … Krigas, N (2022). Facilitating conservation and bridging gaps for the sustainable exploitation of the Tunisian local endemic plant Marrubium aschersonii (Lamiaceae). Sustainability 14:1637. https://doi.org/10.3390/su14031637

Pound LM, Ainsley PJ, Facelli M (2014). Dormancy-breaking and germination requirements for seeds of Acacia papyrocarpa, Acacia oswaldii and Senna artemisioides ssp. coriacea, three Australian arid-zone Fabaceae species. Australian Journal of Botany 62(7):546-557. http://dx.doi.org/10.1071/BT14077

Randell BR (1989). Revision of the Cassiinae in Australia. 2. Senna miller sect. Psilorhegma (J. Vogel) Irwin & Barneby. Journal of the Adelaide Botanic Garden 12:165-272.

Randell BR, Barlow BA (1998). Senna. In: George AS (ex ed) Flora of Australia, vol 12. Australian Government Publishing Service, Canberra. pp 89-138.

RHS Plant Selector (2021). Senna artemisioides. Retrieved 2022 October 01 from https://www.rhs.org.uk/plants/83033/senna-artemisioides/details

Rolston MP (1978). Water impermeable seed dormancy. Botanical Review 44:365-396. https://doi.org/10.1007/BF02957854

Sarasan V, Kite GC, Sileshi GW, Stevenson PC (2011). Applications of phytochemical and in vitro techniques for reducing over-harvesting of medicinal and pesticidal plants and generating income for the rural poor. Plant Cell Reports 30:1163-1172. https://doi.org/10.1007/s00299-011-1047-5

Siddique I, Bukhari NAW, Perveen K, Siddiqui I (2015). Influence of plant growth regulators on in vitro shoot multiplication and plantlet formation in Cassia angustifolia Vahl. Brazilian Archives of Biology and Technology 58:686-691.

Silva SSS, Souza EH, Souza FVD, Max DAS, Rossi ML, Costa MAPC (2021). Post-seminal development and cryopreservation of endemic or endangered bromeliads. Anais da Academia Brasileira Ciências 93(1):20191133. https://doi.org/10.1590/0001-3765202120191133

Soltani A, Galeshi S, Zeinali E, Latifi N (2001). Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea coasts of Iran. Seed Science and Technology 29:653662.

Tsoktouridis G, Krigas N, Sarropoulou V, Kampouropoulou S, Papanastasi K, Grigoriadou K, ... Maloupa E (2019). Micropropagation and molecular characterization of Thymus sibthorpii Benth (Lamiaceae), an aromatic-medicinal thyme with ornamental value and conservation concern. In Vitro Cellular & Developmental Biology-Plant 55(6):647-658. https://doi.org/10.1007/s11627-019-10000-y

Wiszniewska A, Nowak B, Kołton A, Sitek E, Grabski K, Dziurka M … Tukaj Z (2016). Rooting response of Prunus domestica L. micro-shoots in the presence of phytoactive medium supplements. Plant Cell Tissue Organ Culture (PCTOC) 125:163-76. https://doi.org/10.1007/s1124 0-015-0937-6

Zembele ER, Ngulube ES (2022). Effect of seed pretreatment methods on germination and early seedling growth of Senna spectabilis. International Journal of Forestry Research. Article ID 6731479. https://doi.org/10.1155/2022/6731479

Ziv M (1991). Vitrification: morphological and physiological disorders of in vitro plants. In: Micropropagation. Springer Netherlands, pp 45-69.