Assessment of ethnopharmacological potential of Cyperus difformis L. in terms of its’ phytochemistry, antibacterial, antioxidant and anticancer attributes
Keywords:antibacterial, anticancer, antioxidant, Cyperus difformis L., phytochemicals
The present research was carried out on Cyperus difformis L., commonly found sedge weed in rice field to evaluate its’ ethnopharmacological potential in terms of phytochemical constituents, antibacterial, antioxidant and anticancer activities. FTIR spectroscopy of powdered material of plant parts, rhizome (with roots), leaves and flowers showed the presence of three main chemical groups, i.e. -OH (alcohol), -C=O (carbonyl) and -CO-O-CO (anhydride). The phytochemical composition of n-Hexane, chloroform and ethanol crude extracts of same parts analysed by GC-MS and n-Hexane extract of various parts indicated 14 compounds with highest (10) in flowers extract followed by six compounds in chloroform and five in ethanol extracts of plant parts. The antibacterial activity was assessed against Salmonella enterica, E. coli and Staphylococcus aureus by using well diffusion method and similar resistance was shown by n-Hexane and ethanol extracts while noticeable inhibition by chloroform extract, especially by leaves showing zone of inhibition, 16.17±0.52mm, comparable with Gentamycin 18±0.11mm. The antioxidant activity in terms of DPPH Scavenging activity was found higher in n-Hexane and chloroform extracts, especially leaves i.e., 54.6±0.43 & 43.45±0.53 as compared to that of ethanol extracts. The percentage activity was increased with an increase of concentration of extracts. Antiproliferative activity checked by SRB proliferative assay and the crude extracts of plant parts of C. difformis showed good activity against A2780 and HCT116 cancer cells in three days. C. difformis having therapeutic action as well as its’ ethnopharmacological history, being used as fodder, it may be recommended good herbal fodder for the dairy animals.
Adeniyi TA, Adeonipekun PA, Omotayo EA (2014). Investigating the phytochemicals and antimicrobial properties of three sedge (Cyperaceae) Species. Notulae Scientia Biologicae 6(3):276-281. http://dx.doi.org/10.15835/nsb.6.3.9356
Adnan M, Rasul A, Hussain G, Shah MA, Sarfraz I, Nageer B, … Sarkar SD (2021). Physcion and Physcion 8-O-B-D-glycopyranoside, natural anthraquinones with potential anti-cancerous activities. Current Drug Targets 22(5):448-504. https://doi.org/10.2174/1389450121999201013154542
Ahmed F, Urooj A (2010). Glucose lowering, hepatoprotective and hypolipidemic activities of stem bark of Ficus racemosa in streptozotocin induced diabetic rats. Journal of Young Pharmacists 2:160-164. http://dx.doi.org/10.4103/0975-1483.55749
Akalin G, Selamoglu Z (2019). Nutrition and foods for skin health. Journal of Pharmaceutical Care 7(1-2):31-33. https://doi.org/10.18502/jpc.v7i1-2.1620
Aneja K, Joshi R (2009). Antimicrobial activity of Amomum subulatum and Elettaria cardamomum against dental caries-causing microorganisms. Ethnobotanical Leaflets 7:3.
Babu RH, Savithramma DN (2014). Screening of secondary metabolites of underutilized species of Cyperaceae. International Journal of Pharmaceutical Sciences Review and Research 24(2):182-187.
Balch JF, Balch PA (2000). Prescription for Nutritional Healing. Penguin Putnam Inc., New York pp 267-270.
Barr R, Pan RS, Crane FL, Brightman AO, Morre´ DJ (1992). Destruction of vitamin K1 of cultured carrot cells by ultraviolet radiation and its effect on plasma membrane electron transport reactions. Biochemistry International 27(3)449-456.
Boeing JS, Barizao EO, e Silva BC, Montanher PF, de Cinque Almeida V, Visentainer JV (2014). Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: Application of principal component analysis. Chemistry Central Journal 8:48. https://doi.org/10.1186/s13065-014-0048-1
Cambell MM, Sederoff RR (1996). Variation in lignin content and composition (mechanisms of control and implications for the genetic improvement of plants). Plant Physiology 110(1):3-13. https://doi.org/10.1104pp.110.1.3
Dahanukar S, Kulkarni R, Rege N (2000). Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 32(4):81-118.
Dang G, Parekar R, Kamat S, Scindia A, Rege N (2011). Anti-inflammatory activity of Phyllanthus emblica, Plumbago zeylanica and Cyperus rotundus in acute models of inflammation. Phytotherapy Research 25(6):904-908. https://doi.org/10.1002/ptr.3345
Dar RA, Shahnawaz M, Qazi PH (2017). Natural product medicines: A literature update. The Journal of Phytopharmacology 6(6):349-351.
Dharmananda S (2003). Gallnuts and the Uses of Tannins in Chinese Medicine. Institute for Traditional Medicine, Portland, Oregon.
Erdemli ME, Augal H, Ege B, Aksungur Z, Bagand HG, Selamoglu Z (2017). The effect of grape seed extract and low-level laser therapy administration on the liver in experimentally fractured mandible. Journal of Turgut Ozal Medical Center 24(2):127-133. http://dx.doi.org/10.5455/jtomc.2016.12.131
Erdemli ME, Salmas RE, Durdagi S, Akgul H, Demirkol M, Aksungur Z, Selamoglu Z (2018). Biochemical changes induced by grape seed extract and low-level laser therapy administration during the intraoral wound healing in rat liver: an experimental and in silico study. Journal of Biomolecular Structure and Dynamics 36(4):993-1008. https://doi.org/10.1080/07391102.2017.1305297
Fato R, Estornell E, Di Bernardo S, Pallotti F, Castelli GP, Lenaz G (1996). Steady-state kinetics of the reduction of coenzyme Q analogs by complex I (NADH:ubiquinone oxidoreductase) in bovine heart mitochondria and submitochondrial particles. Biochemistry 35:2705-2715. https://doi.org/10.1021/bi9516034
Fransworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z (1985). Medicinal plants in therapy. Bulletin of the World Health Organization 63(6):965-981.
Ganesan S (2004). Ethnomedicinal survey of lower Palni hills of Tamil Nadu. Indian Journal of Traditional Knowledge 3(3):299-304.
Goyal AK, Basistha BC, Sen A, Middha SK (2011). Antioxidant profiling of Hippophaesalicifolia growing in sacred forests of Sikkim, India. Functional Plant Biology 38:697-701. https://doi.org/10.1071/fp11016
Harborne JB (1993). Phytochemistry. Academic Press, London pp 89-131.
Hu C, Kitts DD (2000). Studies on the antioxidant activity of Echinacea root extract. Journal of Agricultural and Food Chemistry 48:1466-1472. https://doi.org/10.1021/jf990677
Iranshahi M, Askari M, Sahebkar A, Adjipavlou-Litina D (2009). Evaluation of antioxidant, anti-inflammatory and lipoxygenase inhibitory activities of the prenylated coumarin umbelliprenin. DARU Journal of Pharmaceutical Sciences 17:99-103.
Jiang D, Rasul A, Batool R, Sarfraz I, Hussain G, Tahir MM, … Li X (2019). Potential anticancer properties and mechanisms of action of formononetin. Biomed Research International. 5854315. http://doi.org/10.1155/2019/5854315
Kakarla L, Othayoth R, Botlagunta M (2016). Comparative biochemical studies on Indian sedges Cyperus scar¬iosus R.Br and Cyperus rotundus L. Pharmacognosy Journal 8(6):598-609. http://dx.doi.org/10.5530/pj.2016.6.14
Khan UM, Sevindik M, Zarrabi A, Nami M, Ozdemir B, Kaplan DN, … Sharifi-Rad J (2021). Lycopene: food sources, biological activities, and human health benefits. Oxidative Medicine and Cellular Longevity 2021:2713511. https://doi.org/10.1155.2021.2713511
Kilani-Jaziri S, Neffati A, Limem I, Boubaker J, Skandrani I, Sghair MB (2009). Relationship correlation of antioxidant and antiproliferative capacity of Cyperus rotundus products towards K562 erythroleukemia cells. Chemico-Biological Interactions 181:85-94. https://doi.org/10.1016/j.cbi.2009.04.014
Koes RE, Quattrocchio R, Mol JNM (1994). The flavonoid biosynthetic pathway in plants: Function and evolution. BioEssays 16:123-32. https://doi.org/10.1002/bies.950160209
Koleva II, Van Beek TA, Linseen JPH, de Groot A, Evstatieva LN (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13:8-17. https://doi.org/10.1002/pca.611
Kukkenon I (2001). Flora of Pakistan (Cyperaceae). No. 206. Pakistan Agriculture Research Council, Islamabad.
Kumar S, Paul S, Walia YK, Kumar A, Singhal P (2015). Therapeutic potential of medicinal plants: a review. Journal of Biological and Chemical Chronicles 1(1):46-54.
Larridon I, Reynders M, Huygh W, Bauters K, Putte KVD, Muasya AM, … Goetghebeur P (2011). Affinities in C3 Cyperus lineages (cyperaceae) revealed using molecular phylogenetic data and carbon isotope analysis. Botanical Journal of the Linnean Society 167:19-46. https://doi.org/10.1111/j.1095-8339.2011.01160.x
Manzoor S, Bilal A, Khan S, Rahimullah, Iftikhar S, Emwas A, … Faisal A (2018). Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Scientific Reports 8(1):3305. https://doi.org/10.1038/s41598-018-21642-0
Middha SK, Usha T, Babu D, Misra AK, Lokesh P, Goyal AK (2016). Evaluation of antioxidative, analgesic and anti-inflammatory activities of methanolic extract of Myrica nagi leaves – an animal model approach. Symbiosis 70:179-84. http://dx.doi.org/10.1007/s13199-016-0422-y
Middha SK, Usha T, Pande V (2013). HPLC evaluation of phenolic profile, nutritive content and antioxidant capacity of extracts obtained from Punica granatum fruit peel. Advances in Pharmacological Sciences 1:296236. https://doi.org/10.1155/2013/296236
Murugan R, Prabu J, Chandran R, Sajeesh T, Iniyavan M, Parimelazhagan T (2016). Nutritional composition, in vitro antioxidant and anti-diabetic potentials of Breynia retusa (Dennst.) Alston. Food Science and Human Wellness 5:30-8. https://doi.org/10.1016/j.fshw.2015.12.001
Naczk M, Shahidi F (2006). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis 41:1523-42. https://doi.org/10.1016/j.jpba.2006.04.002
Nageen B, Sarfraz I, Rasul A, Hussain G, Rukhsar F, Irshad S, … Ali M (2018). Eupatilin: a natural pharmacologically active flavone compound with its wide range applications. Journal of Asian Natural Products Research 22(1):1-16. https://doi.org/10.1080/10286020.2018.1492565
Nawaz J, Rasul A, Shah MA, Hussain G, Riaz A, Sarfraz I, Zafar S, Adnan M, khan AH, Selamoglu Z (2020). Cardamonin: A new player to fight cancer via multiple cancer signaling pathways. Life sciences 250: 117591. https://doi.org/10.1016/j.lfs.2020.117591
Ozdemir B, Gulhan MF, Sahna E, Selamoglu Z (2021). The investigation of antioxidant and anti-inflammatory potentials of apitherapeutic agents on heart tissues in nitric oxide synthase inhibited rats via Na-nitro-L-arginine methyl ester. Clinical and Experimental Hypertension 243(1):69-76. https://doi.org/10.1080/10641963.2020.1806294
Paliwal SK, Sati B, Samriti F, Sharma S (2017). Antioxidant and antibacterial activities of various extracts of Inula cuspidate C.B. Clarke stem. Journal of Basic and Applied Science 6(2):97-105. http://dx.doi.org/10.1016/j.bjbas.2016.10.003
Peerzada AM (2017). Biology, agricultural impact, and management of Cyperus rotundus L.: the world’s most tenacious weed. Acta Physiologiae Plantarum 39(12). https://doi.org/10.1007/s11738-017-2574-7
Peerzada AM, Ali HH, Naeem M, Latif M, Bukhari AH, Tanveer A (2015). Cyperus rotundus L.: Traditional uses, phytochemistry, and pharmacological activities. Journal of Ethnopharmacology 174:540-560. https://doi.org/10.1016/j.jep.2015.08.012
Pharmawati M, Wrasiati LP (2020). Phytochemical screening and FTIR spectroscopy on crude extract from Enhalus acoroides leaves. Malaysian Journal of Analytical Sciences 24(1):70-77.
Pierpoint WS (2000). Why do plants make medicines? Biochemist 22:37-40.
Russell A, Abo-Altemen, Al-Shammari AM, Shawkat MS (2019). GC-MS analysis and chemical composition identification of Cyperus rotandus L. from Iraq. Energy Procedia 157:1462-1474. https://doi.org/10.1016/j.egypro.2018.11.311
Salehi B, Selamoglu Z, Sener B, Kilic M, Jugran AK, De Tommasi N, … Morais-Braga MFB (2019). Berberis plants-drifting from farm to food applications, phytotherapy, and phytopharmacology. Foods 8(10):522-549. https://doi.org/10.3390/foods8100522
Selamoglu Z (2017). Biotechnological approaches on anticancer activity of flavonoids-mini review. Modern Approaches in Drugs Designing 1(2):510. http://dx.doi.org/10.31031/MADD.2017.01.000510
Selamoglu Z (2017). Polyphenolic compound in human health with pharmacology properties. Journal of Traditional Medicine Clinical Naturopathy 6:4. https://doi.org/10.4172/2573-4555.1000e138
Selamoglu Z (2018). The natural products and healthy life. Journal of Traditional Medicine Clinical Naturopathy 7:2. https://doi.org/10.4172/2573-4555.1000e146
Selamoglu Z, Dusgan C, Akgul H, Gulhan MF (2017). In-vitro antioxidant activities of the ethanolic extracts of some contained-allantoin plants. Iranian Journal of Pharmaceutical Research 16:92-98.
Shetly BS, Udapa SL, Udapa AL, Somayaji SN (2006). Effect of Centella asiatica L. (Umbelliferae) on normal and dexamethasone-supressed wound healing in Wistar albino rats. The International Journal of Lower Extremity Wounds 5(3):137-143. https://doi.org/10.1177/1534734606291313
Siddhuraju P, Becker K (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera lam.) leaves. Journal of Agricultural and Food Chemistry 51(8):2144-55. https://doi.org/10.1021/jf020444
Singh N, Pandey BR, Verma P, Bhalla M, Gilca M (2012). Phyto-pharmacotherapeutics of Cyperus rotundus Linn. (Motha): an overview. Indian Journal of Natural Products and Resources 3:467-76.
Srivastava R, Singh A, Shukla S (2013). Chemical investigation and pharmaceutical action of Cyperus rotundus - a review. Journal of Biologically Active Products from Nature 3(3):166-172. https://doi.org/10.1080/22311866.2013.833381
Suresh PK, Sucheta S, Sudarshana VD, Selvamani P, Latha S (2008). Antioxidant activity in some selected Indian medicinal plants. African Journal of Biotechnology 7:1826-1828. https://doi.org/10.5897/AJB2008.000-5030
Taheri Y, Herrera-Bravo J, Huala L, Salazar LA, Sharifi-Rad J, Akram M, … Cho WC (2021). Cyperus spp.: A review on phytochemical composition, biological activity, and health- promoting effects. Oxidative Medicine and Cellular Longevity 2021:4014867. https://doi.org/10.1155/2021/4014867
Udari LM (2018). Medicinal properties of Cyperus species (Sedge Family, Cyperaceae). Master’s Thesis, Eastern Illinois University, Charleston, Illinois.
Usha T, Middha SK, Bhattacharya M, Lokesh P, Goyal AK (2014). Rosmarinic acid, a new polyphenol from Baccaurea ramiflora lour. leaf: A Probable compound for its anti-inflammatory activity. Antioxidants (Basel) 3:830-42. https://doi.org/10.3390%2Fantiox3040830
Villano D, Fernandez-Pachon MS, Moya ML, Troncoso AM, Garcia-Parrilla MC (2007). Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 71:230-235. https://doi.org/10.1016/j.talanta.2006.03.050
Viuda-Martos M, Fernandez-Lopez J, Perez-Alvarez JA (2010). Pomegranate and its many functional components as related to human health: A review. Comprehensive Reviews in Food Science and Food Safety 9:635-54. https://doi.org/10.1111/j.1541-4337.2010.00131.x
Weiss RF, Fintelmann R (2000). Herbal Medicine. Thieme Medical Publishers, Stuttgart pp 3-20.
Zheng W, Wang SY (2001). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry 49:5165-5170. https://doi.org/10.1021/jf010697n
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