Assessment of ethnopharmacological potential of Cyperus difformis L. in terms of its’ phytochemistry, antibacterial, antioxidant and anticancer attributes


  • Shahzada Z. AHMAD Government College University, Department of Botany, Lahore 54000 (PK)
  • Zaheer ud din KHAN Government College University, Department of Botany, Lahore 54000 (PK)
  • Safdar A. MIRZA Government College University, Department of Botany, Lahore 54000 (PK)



antibacterial, anticancer, antioxidant, Cyperus difformis L., phytochemicals


The present research was carried out on Cyperus difformis L., commonly found sedge weed in rice field to evaluate its’ ethnopharmacological potential in terms of phytochemical constituents, antibacterial, antioxidant and anticancer activities. FTIR spectroscopy of powdered material of plant parts, rhizome (with roots), leaves and flowers showed the presence of three main chemical groups, i.e. -OH (alcohol), -C=O (carbonyl) and -CO-O-CO (anhydride). The phytochemical composition of n-Hexane, chloroform and ethanol crude extracts of same parts analysed by GC-MS and n-Hexane extract of various parts indicated 14 compounds with highest (10) in flowers extract followed by six compounds in chloroform and five in ethanol extracts of plant parts. The antibacterial activity was assessed against Salmonella enterica, E. coli and Staphylococcus aureus by using well diffusion method and similar resistance was shown by n-Hexane and ethanol extracts while noticeable inhibition by chloroform extract, especially by leaves showing zone of inhibition, 16.17±0.52mm, comparable with Gentamycin 18±0.11mm. The antioxidant activity in terms of DPPH Scavenging activity was found higher in n-Hexane and chloroform extracts, especially leaves i.e., 54.6±0.43 & 43.45±0.53 as compared to that of ethanol extracts. The percentage activity was increased with an increase of concentration of extracts. Antiproliferative activity checked by SRB proliferative assay and the crude extracts of plant parts of C. difformis showed good activity against A2780 and HCT116 cancer cells in three days. C. difformis having therapeutic action as well as its’ ethnopharmacological history, being used as fodder, it may be recommended good herbal fodder for the dairy animals.


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How to Cite

AHMAD, S. Z., KHAN, Z. ud din, & MIRZA, S. A. (2022). Assessment of ethnopharmacological potential of Cyperus difformis L. in terms of its’ phytochemistry, antibacterial, antioxidant and anticancer attributes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12918.



Research Articles
DOI: 10.15835/nbha50312918