Arsenate reductase gene from Pityrogramma calomelanos L.  enhances tolerance to arsenic in tobacco

Thuy Thi Bich LE; Huong Thi HO; Trang Thu DAO; Ngoc Thy NGUYEN; Luong Thi TRAN; Linh Thi Thuy NGO; Tru VAN NGUYEN

doi:10.15835/nbha51212896

Authors

Thuy Thi Bich LE Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Huong Thi HO Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Trang Thu DAO Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Ngoc Thy NGUYEN Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi; University of Science and Technology of Hanoi, Vietnam Academy of Science and Technolog, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Luong Thi TRAN Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Linh Thi Thuy NGO Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN)
Tru VAN NGUYEN Vietnam Academy of Science and Technology, Instute of Biotechnology, Department of Plant Cell Genetic, 18 Hoang Quoc Viet, Cau Giay, Hanoi; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi (VN) https://orcid.org/0000-0001-6669-9094

DOI:

https://doi.org/10.15835/nbha51212896

Keywords:

ArsC, arsenic tolerance, phytoremediation, Pityrogramma calomelanos L.

Abstract

Arsenic (As) contamination in soil, water and air is an alarming issue worldwide and has serious effects on human health and environment. Arsenic is a naturally occurring element found in rocks, soil, and water, and exposure to high levels of arsenic can lead to a range of health problems. The effects of arsenic contamination can also be felt in the environment, as it can harm plants and animals and disrupt ecological systems. The major purpose of this study was to produce transgenic plants with improved tolerance to and accumulation of arsenic via transformation of arsenate reductase gene (ArsC) into tobacco genome. Transgenic plants were screen by PCR and southern blot. Further, their tolerance and accumulation to arsenic were evaluated. In the result, we have cloned, characterized, and transformed the ArsC gene from Pityrogramma calomelanos L. (PcArsC). Its phylogenetic analysis revealed 99% homology to ArsC gene in GenBank (accession number X80057.1). Moreover, Southern blot analysis showed that ArsC gene was integrated into the tobacco genome as a single-copy. These single-copy transgenic lines showed much higher tolerance to and accumulation of As than wild type, with no other phenotypes observed. These results demonstrated that Pityrogramma calomelanos ArsC gene can improve arsenic tolerance and accumulation in transgenic tobacco lines. Thus, using Pityrogramma calomelanos L. ArsC gene for genetic engineering has potential implications in the decontamination of arsenic-containing soil.

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