Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions


  • Lina M. ABU-ZIADA Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
  • Samia A. HAROUN Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
  • Rasha M.E. GAMEL Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
  • Mohamed A. ABBAS Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
  • Mahmoud R. SOFY Al Azhar University, Faculty of Science, Botany and Microbiology Department, 11884 Nasr City, Cairo (EG)
  • Heba Y. EL-BANNA Mansoura University, Faculty of Agriculture, Vegetable and Floriculture Department, 35516 Mansoura (EG)



benzyl aminopurine, light-emitting diode systems (LEDs), Moringa oleifera, secondary metabolites, thidiazuron, tissue culture


The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vitro culture. Two types of cytokinin: 6-benzylaminopurin (BAP), and thidiauron (TDZ) singly at three different concentrations (0, 1, or 2 mg/L), were used, as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). After incubation for 30 days, the obtained results showed that the survival percentage increased by the treatments with the maximum value (85%) by MS medium supplemented with 2 mg/L TDZ, under violet LEDs illumination. However, the addition of thidiazuron (TDZ) to the medium did not propagate shoot, and this treatment recorded 100% callus formation other than BAP. The maximum number of axillary shoots per explant and the number of leaves recorded in the MS medium contained 2 mg/L BAP under violet LEDs. Compared to the control values, all the used treatments generally caused surprisingly stimulating the determined antioxidant enzymes and compounds (peroxidase, polyphenol oxidase &catalase and phenols, flavonoids, anthocyanin& ascorbic acid). Concerning the phytohormone content of the hormone-free medium (control), IAA, kinetin, and zeatin increased. Meanwhile, GA3 and ABA decreased with the used treatments.


Abu-Shahba MS, Mansour MM, Mohamed HI, Sofy MR (2022). Effect of biosorptive removal of cadmium ions from hydroponic solution containing indigenous garlic peel and mercerized garlic peel on lettuce productivity. Scientia Horticulturae 293:110727.

Ahmadi T, Shabani L, Sabzalian MR (2021). LED light sources improved the essential oil components and antioxidant activity of two genotypes of lemon balm (Melissa officinalis L.). Botanical Studies 62(1):1-13.

Al-Mayahi AMW (2016). Effect of red and blue light emitting diodes “CRB-LED” on in vitro organogenesis of date palm (Phoenix dactylifera L.) cv. Alshakr. World Journal of Microbiology and Biotechnology 32(10):1-8.

ALHaithloul HAS, Khan MI, Musa A, Ghoneim MM, ALrashidi AA, Khan I, Azab E, Gobouri AA, Sofy MR, El-Sherbiny M (2022). Phytotoxic effects of Acacia saligna dry leachates on germination, seedling growth, photosynthetic performance, and gene expression of economically important crops. PeerJ 10:e13623.

Anwar F, Latif S, Ashraf M, Gilani AH (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 21(1):17-25.

Azmi NS, Ahmad R, Ibrahim R (2014). Effects of red and blue (RB) LED on the in vitro growth of Rosa kordesii in multiplication phase. In: 2nd International Conference on Agriculture and Biotechnology. Singapore.

Babar H, Rizwan B, Babar A, Nazia H, Noreen S, Naeem N, Raza F, Seed Z, Imran S (2022). Therapeutic effect of Moringa oleifera: A review. Pakistan BioMedical Journal 10-13.

Badawy IH, Hmed AA, Sofy MR, Al-Mokadem AZ (2022). Alleviation of cadmium and nickel toxicity and phyto-stimulation of tomato plant by endophytic Micrococcus luteus and Enterobacter cloacae. Plants 11(15):2018.

Bajji M, Lutts S, Kinet J-M (2000). Physiological changes after exposure to and recovery from polyethylene glycol-induced water deficit in callus cultures issued from durum wheat (Triticum durum Desf.) cultivars differing in drought resistance. Journal of Plant Physiology 156(1):75-83.

Barceló-Muñoz A, Barceló-Muñoz M, Gago-Calderon A (2021). Effect of LED lighting on physical environment and microenvironment on in vitro plant growth and morphogenesis: the need to standardize lighting conditions and their description. Plants 11(1):60.

Basra S, Zahar M, Rehman H, Yasmin A, Munir H (2009). Evaluating the response of sorghum and moringa leaf water extracts on seedling growth in hybrid maize. In: Proceedings of the International Conference on Sustainable Food Grain Production: Challenges and Opportunities. University of Agriculture, Faisalabad, Pakistan, pp 22.

Batista DS, Felipe SHS, Silva TD, de Castro KM, Mamedes-Rodrigues TC, Miranda NA, … Chagas K (2018). Light quality in plant tissue culture: does it matter? In Vitro Cellular & Developmental Biology-Plant 54(3):195-215.

Bazin J, Langlade N, Vincourt P, Arribat S, Balzergue S, El-Maarouf-Bouteau H, Bailly C (2011). Targeted mRNA oxidation regulates sunflower seed dormancy alleviation during dry after-ripening. The Plant Cell 23(6):2196-2208.

Bidabadi SS, Jain SM (2020). Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants 9(6):702.

Cavallaro V, Pellegrino A, Muleo R, Forgione I (2022). Light and plant growth regulators on in vitro proliferation. Plants 11(7):844.

Chalker‐Scott L (1999). Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology 70(1):1-9.

Chen X-l, Guo W-z, Xue X-z, Wang L-c, Qiao X-j (2014). Growth and quality responses of ‘Green Oak Leaf’lettuce as affected by monochromic or mixed radiation provided by fluorescent lamp (FL) and light-emitting diode (LED). Scientia Horticulturae 172:168-175.

Chen Y, Cao X, Lu Y, Wang X (2000). Effects of rare earth metal ions and their EDTA complexes on antioxidant enzymes of fish liver. Bulletin of Environmental Contamination and Toxicology 65(3):357-365.

Cheng ZJ, Wang L, Sun W, Zhang Y, Zhou C, Su YH, Li W, Sun TT, Zhao XY, Li XG (2013). Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3. Plant Physiology 161(1):240-251.

Cioć M, Dziurka M, Pawłowska B (2022). Changes in Endogenous phytohormones of Gerbera jamesonii axillary shoots multiplied under different light emitting diodes light quality. Molecules 27(6):1804.

Dawood MF, Abu-Elsaoud AM, Sofy MR, Mohamed HI, Soliman MH (2022a). Appraisal of kinetin spraying strategy to alleviate the harmful effects of UVC stress on tomato plants. Environmental Science and Pollution Research:1-21.

Dawood MF, Sofy MR, Mohamed HI, Sofy AR, Abdel-kader HA (2022b). Hydrogen sulfide modulates salinity stress in common bean plants by maintaining osmolytes and regulating nitric oxide levels and antioxidant enzyme expression. Journal of Soil Science and Plant Nutrition 1-19.

Egbuna C, Ifemeje JC, Maduako MC, Tijjani H, Udedi SC, Nwaka AC, Ifemeje MO (2018) Phytochemical test methods: qualitative, quantitative and proximate analysis. Phytochemistry. Apple Academic Press, pp 381-426.

El-Sheshtawy HS, Mahdy HM, Sofy AR, Sofy MR (2022). Production of biosurfactant by Bacillus megaterium and its correlation with lipid peroxidation of Lactuca sativa. Egyptian Journal of Petroleum 31(2):1-6.

El-Sheshtawy HS, Sofy MR, Ghareeb DA, Yacout GA, Eldemellawy MA, Ibrahim BM (2021). Eco-friendly polyurethane acrylate (PUA)/natural filler-based composite as an antifouling product for marine coating. Applied Microbiology and Biotechnology 105(18):7023-7034.

Fotouo-M H, du Toit ES, Robbertse PJ (2015). Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage. AoB Plants 7.

Fouda HM, Sofy MR (2022). Effect of biological synthesis of nanoparticles from Penicillium chrysogenum as well as traditional salt and chemical nanoparticles of zinc on canola plant oil productivity and metabolic activity. Egyptian Journal of Chemistry 65(3):1-2.

Fu S-F, Wei J-Y, Chen H-W, Liu Y-Y, Lu H-Y, Chou J-Y (2015). Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms. Plant Signaling & Behavior 10(8):e1048052.

Galán-Ávila A, García-Fortea E, Prohens J, Herraiz FJ (2020). Development of a direct in vitro plant regeneration protocol from Cannabis sativa L. seedling explants: developmental morphology of shoot regeneration and ploidy level of regenerated plants. Frontiers in Plant Science 11:645.

Galicia LN, Rosales E, A Palacios Rojas N (2009) Laboratory protocols 2008: Maize nutrition quality and plant tissue analysis laboratory. Cimmyt.

Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA (1996). Plant hormones and plant growth regulators in plant tissue culture. In vitro Cellular & Developmental Biology-Plant 32(4):272-289.

Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. John Wiley & Sons.

Gopalakrishnan L, Doriya K, Kumar DS (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness 5(2):49-56.

Grzegorczyk-Karolak I, Hnatuszko-Konka K, Krzemińska M, Olszewska MA, Owczarek A (2021). Cytokinin-based tissue cultures for stable medicinal plant production: regeneration and phytochemical profiling of Salvia bulleyana shoots. Biomolecules 11(10):1513.

Guo B, Abbasi BH, Zeb A, Xu L, Wei Y (2011). Thidiazuron: a multi-dimensional plant growth regulator. African Journal of Biotechnology 10(45):8984-9000.

Gupta S, Kachhwaha S, Kothari SL, Jain R (2020). Synergistic effect of cytokinins and auxins enables mass clonal multiplication of drumstick tree (Moringa oleifera Lam.): a wonder. In Vitro Cellular & Developmental Biology-Plant 56(4):458-469.

Hisano H, Matsuura T, Mori IC, Yamane M, Sato K (2016). Endogenous hormone levels affect the regeneration ability of callus derived from different organs in barley. Plant Physiology and Biochemistry 99:66-72.

Hönig M, Plíhalová L, Husičková A, Nisler J, Doležal K (2018). Role of cytokinins in senescence, antioxidant defence and photosynthesis. International Journal of Molecular Sciences 19(12):4045.

Huang X, Hou L, Meng J, You H, Li Z, Gong Z, Yang S, Shi Y (2018). The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis. Molecular Plant 11(7):970-982.

Joo JH, Bae YS, Lee JS (2001). Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiology 126(3):1055-1060.

Kapoor S, Raghuvanshi R, Bhardwaj P, Sood H, Saxena S, Chaurasia OP (2018). Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew. Journal of Photochemistry and Photobiology B: Biology 183:258-265.

Kar M, Mishra D (1976). Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology 57(2):315-319.

Kawano T (2003). Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Reports 21(9):829-837.

Khan T, Khan MA, Karam K, Ullah N, Mashwani Z-u-R, Nadhman A (2021). Plant in vitro culture technologies; a promise into factories of secondary metabolites against COVID-19. Frontiers in Plant Science 12:356.

Kim K, Kook H, Jang Y, Lee W, Kamala-Kannan S, Chae J, Lee K (2013). The effect of blue-light-emitting diodes on antioxidant properties and resistance to Botrytis cinerea in tomato. Journal of Plant Pathology and Microbiology 4(9).

Knegt E, Bruinsma J (1973). A rapid, sensitive and accurate determination of indolyl-3-acetic acid. Phytochemistry 12(4):753-756.

Kousalya L, Bai VN (2016). Effect of growth regulators on rapid micropropagation and antioxidant activity of Canscora decussata (Roxb.) Roem. & Schult.–A threatened medicinal plant. Asian Pacific Journal of Reproduction 5(2):161-170.

Lappas CM (2015). The plant hormone zeatin riboside inhibits T lymphocyte activity via adenosine A2A receptor activation. Cellular & Molecular Immunology 12(1):107-112.

Lee B, Martin P, Bangerth F (1989). The effect of sucrose on the levels of abscisic acid, indoleacetic acid and zeatin/zeatin riboside in wheat ears growing in liquid culture. Physiologia Plantarum 77(1):73-80.

Lee C, Lee Y, Kuo H-C, Tsai M, Lu T, Wang S (2008). High brightness GaN-based flip-chip light-emitting diodes by adopting geometric sapphire shaping structure. Semiconductor Science and Technology 23(2):025015.

Lemma DT, Banjaw DT, Megersa HG (2020). Micropropagation of medicinal plants. International Journal of Plant Breeding and Crop Science 7:796-802.

Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International Journal of Molecular Sciences 16(6):12791-12835.

Leshem B, WERKER E, Shalev D (1988). The effect of cytokinins on vitrification in melon and carnation. Annals of Botany 62(3):271-276.

Li S, Xue L, Xu S, Feng H, An L (2007). Hydrogen peroxide involvement in formation and development of adventitious roots in cucumber. Plant Growth Regulation 52(2):173-180.

Lin M, Zhang J, Chen X (2018). Bioactive flavonoids in Moringa oleifera and their health-promoting properties. Journal of Functional Foods 47:469-479.

Mahood HE, Alwash BM, Ibrahim KM (2018). Improvement of alkaloids yield using phenylalanine as a precursor supplemented to Moringa oleifera L. callus cultures. Biochemical and Cellular Archives 18(Suppl. 1):913-919.

Maksoud MA, Bekhit M, El-Sherif DM, Sofy AR, Sofy MR (2022). Gamma radiation-induced synthesis of a novel chitosan/silver/Mn-Mg ferrite nanocomposite and its impact on cadmium accumulation and translocation in brassica plant growth. International Journal of Biological Macromolecules 194:306-316.

Marrazzo P, O’Leary C (2020). Repositioning natural antioxidants for therapeutic applications in tissue engineering. Bioengineering 7(3):104.

Mathur M, Yadav S, Katariya PK, Kamal R (2014). In vitro propagation and biosynthesis of steroidal sapogenins from various morphogenetic stages of Moringa oleifera Lam., and their antioxidant potential. Acta Physiologiae Plantarum 36(7):1749-1762.

Merzlyak MN, Chivkunova OB, Solovchenko AE, Naqvi KR (2008). Light absorption by anthocyanins in juvenile, stressed, and senescing leaves. Journal of Experimental Botany 59(14):3903-3911.

Mohamed HI, Abd-Elsalam KA, Tmam AM, Sofy MR (2021) Silver-based nanomaterials for plant diseases management: Today and future perspectives. Silver Nanomaterials for Agri-Food Applications. Elsevier, pp 495-526.

Moraes RM, Cerdeira AL, Lourenço MV (2021). Using micropropagation to develop medicinal plants into crops. Molecules 26(6):1752.

Mourad AM, Amin AE-EAZ, Dawood MF (2021). Genetic variation in kernel traits under lead and tin stresses in spring wheat diverse collection. Environmental and Experimental Botany 192:104646.

Mowafy A, Agha M, Haroun S, Abbas M, Elbalkini M (2022). Insights in nodule-inhabiting plant growth promoting bacteria and their ability to stimulate Vicia faba growth. Egyptian Journal of Basic and Applied Sciences 9(1):51-64.

Mukherjee S, Choudhuri M (1983). Implications of water stress‐induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia plantarum 58(2):166-170.

Muslihatin W, Jadid N, Saputro T, Purwani K, Himayani C, Calandry A Characteristic of synthetic seeds from two medicinal plants (Moringa oleifera and Camellia sinensis). In: Journal of Physics: Conference Series 1040:012005.

Naik SK, Chand PK (2006). Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Scientia Horticulturae 108(3):247-252.

Neill SO, Gould KS (2003). Anthocyanins in leaves: light attenuators or antioxidants? Functional Plant Biology 30(8):865-873.

North JJ, Ndakidemi PA, Laubscher CP (2012). Effects of antioxidants, plant growth regulators and wounding on phenolic compound excretion during micropropagation of Strelitzia reginae. International Journal of the Physical Sciences.

Nouman W, Siddiqui MT, Basra S, Ahmed M (2012). Moringa oleifera leaf extract: An innovative priming tool for rangeland grasses. Turkish Journal of Agriculture and Forestry 36(1):65-75.

Ogita S, Miyazaki J, Godo T, Kato Y (2009). Possibility for selective accumulation of polyphenolics in tissue cultures of senno (Lychnis senno Siebold et Zucc.). Natural Product Communications 4(3):1934578X0900400312.

Olle M, Viršile A (2013). The effects of light-emitting diode lighting on greenhouse plant growth and quality. Agricultural and Food Science 22(2):223-234.

Pham TL, Nguyen VH, Hoang TLT, Ha TTT, Tran TK, Vu XD, Cao PB, Nguyen QT (2020). Micropropagation from root segments to improve seedling quality in Chinese foxglove crops. Journal of Plant Biotechnology 47(3):235-241.

Phua QY, Subramaniam S, Lim V, Chew BL (2018). The establishment of cell suspension culture of sabah snake grass (Clinacanthus nutans (Burm. F.) Lindau). In Vitro Cellular & Developmental Biology-Plant 54(4):413-422.

Ragaey MM, Sadak MS, Dawood MF, Mousa NH, Hanafy RS, Latef AAHA (2022). Role of signaling molecules sodium nitroprusside and arginine in alleviating salt-induced oxidative stress in wheat. Plants 11(14):1786.

Ramírez-Mosqueda M, Cruz-Cruz C, Atlahua-Temoxtle J, Bello-Bello J (2019). In vitro conservation and regeneration of Laelia anceps Lindl. South African Journal of Botany 121:219-223.

Ridzuan N, Abdullah N, Vun Y, Supramaniam C (2020). Micropropagation and defence enzymes assessment of Moringa oleifera L. plantlets using nodal segments as explant. South African Journal of Botany 129:56-61.

Saeedpour A, Godehkahriz SJ, Lohrasebi T, Esfahani K, Salmanian AH, Razavi K (2021). The effect of endogenous hormones, total antioxidant and total phenol changes on regeneration of barley cultivars. Iranian Journal of Biotechnology 19(1):e2838.

Safi-naz S, Rady MM (2015). Moringa oleifera leaf extract improves growth, physio-chemical attributes, antioxidant defence system and yields of salt-stressed Phaseolus vulgaris L. plants. International Journal of ChemTech Research 8(11):120-134.

Saini R, Saad K, Ravishankar G, Giridhar P, Shetty N (2013). Genetic diversity of commercially grown Moringa oleifera Lam. cultivars from India by RAPD, ISSR and cytochrome P450-based markers. Plant Systematics and Evolution 299(7):1205-1213.

Saini R, Shetty N, Giridhar P, Ravishankar G (2012). Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants. 3 Biotech 2(3):187-192.

Sang YL, Cheng ZJ, Zhang XS (2018). Plant stem cells and de novo organogenesis. New Phytologist 218(4):1334-1339.

Sheteiwy MS, Ulhassan Z, Qi W, Lu H, AbdElgawad H, Minkina T, … Dawood M (2022). Association of jasmonic acid priming with multiple defense mechanisms in wheat plants under high salt stress. Frontiers in Plant Science 13: 886862.

Sofy M, Mohamed H, Dawood M, Abu-Elsaoud A, Soliman M (2021). Integrated usage of Trichoderma harzianum and biochar to ameliorate salt stress on spinach plants. Archives of Agronomy and Soil Science 7:1-22.

Steinitz B, Tabib Y, Gaba V, Gefen T, Vaknin Y (2009). Vegetative micro-cloning to sustain biodiversity of threatened Moringa species. In Vitro Cellular & Developmental Biology-Plant 45(1):65-71.

Stephenson KK, Fahey JW (2004). Development of tissue culture methods for the rescue and propagation of endangered Moringa spp. germplasm. Economic Botany 58(1):S116-S124.[S116:DOTCMF]2.0.CO;2

Tibbitts T, Morgan D, Warrington I (1983). Growth of lettuce, spinach, mustard, and wheat plants under four combinations of high-pressure sodium, metal halide, and tungsten halogen lamps at equal PPFD. Journal of American Society of Horticultural Sciences 108(4):622-630.

Torrizo L, Zapata F (1986). Anther culture in rice: IV. The effect of abscisic acid on plant regeneration. Plant Cell Reports 5(2):136-139.

Touaibia M, Chaouch F (2017). Propriétés antioxydantes et antimicrobiennes des extraits de Myrtus nivellei Batt et Trab. obtenus in situ et in vitro. Phytothérapie 15(1):16-22.

Traore A, Maximova SN, Guiltinan MJ (2003). Micropropagation of Theobroma cacao L. using somatic embryo-derived plants. In Vitro Cellular & Developmental Biology-Plant 39(3):332-337.

Vats S, Gupta T (2017). Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India. Physiology and molecular biology of plants 23(1):239-248.

Vendrame WA, Xu J, Beleski D (2022). Evaluation of the effects of culture media and light sources on in vitro growth of Brassavola nodosa (L.) Lindl. hybrid. Horticulturae 8(5):450.

Vera-Sirera F, Gomez MD, Perez-Amador MA (2016) DELLA proteins, a group of GRAS transcription regulators that mediate gibberellin signaling Plant transcription factors. Elsevier, pp 313-328.

Vongsak B, Sithisarn P, Mangmool S, Thongpraditchote S, Wongkrajang Y, Gritsanapan W (2013). Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Industrial Crops and Products 44:566-571.

Wang S, Yang C, Tu H, Zhou J, Liu X, Cheng Y, Luo J, Deng X, Zhang H, Xu J (2017). Characterization and metabolic diversity of flavonoids in citrus species. Scientific Reports 7(1):1-10.

Weiss D, Ori N (2007). Mechanisms of cross talk between gibberellin and other hormones. Plant Physiology 144(3):1240-1246.

Younes NA, Rahman M, Wardany AA, Dawood MF, Mostofa MG, Keya SS, Abdel Latef AAH, Tran L-SP (2021). Antioxidants and bioactive compounds in licorice root extract potentially contribute to improving growth, bulb quality and yield of onion (Allium cepa). Molecules 26(9):2633.

Yu L, Song CM, Sun LJ, Li LL, Xu ZG, Tang CM (2020). Effects of light-emitting diodes on tissue culture plantlets and seedlings of rice (Oryza sativa L.). Journal of Integrative Agriculture 19(7):1743-1754.

Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4):555-559.

Zimmerman TW, Scorza R (1992). Shoot growth and proliferation of peach under varying environmental regimes. HortScience 27(6):696a-696.

Zullaikah S, Naulina R, Meinawati P, Fauziyah K, Rachimoellah M, Rachmaniah O, … Prasetyo E (2019). Enhanced Extraction of phenolic compounds from Moringa oleifera leaves using subcritical water ethanol mixture. In: IOP Conference Series: Materials Science and Engineering. 543:012021.



How to Cite

ABU-ZIADA, L. M., HAROUN, S. A., GAMEL, R. M., ABBAS, M. A., SOFY, M. R., & EL-BANNA, H. Y. (2022). Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12879.



Research Articles
DOI: 10.15835/nbha50312879