The effects of strigolactones on some biochemical traits in calcified media on grapevine


  • Emine Sema ÇETİN University of Yozgat Bozok, Faculty of Agriculture, Department of Horticulture, 66100, Yozgat (TR)
  • Birol KOÇ University of Yozgat Bozok, School of Graduate Studies, Department of Horticulture, 66100, Yozgat (TR)



calcium oxide, grapevine, mineral compounds, phenolic compounds, stress, strigolactone


Plants are stressed in different ways when they are in environments unsuitable for them. Among these stresses, abiotic factors are common. Calcified soil is also a stress factor. In this type of soil, there are problems with the nutrient intake of the plant, nutrient deficiencies, or toxicity. In a stress environment, plants try to survive by reacting differently. One of these reactions is that plants increase the synthesis of phenolic compounds. These compounds perform various physiological functions in adapting to environmental problems. It is known that the reactions of plants to stress are associated with endogenous hormones. One of these hormones is strigolactone (SL), which is produced in plant roots and introduced as a new generation of hormones. In this study, the effects of SL applications in grapevine grown in calcified environments were examined with regard to the content of phenolic compounds and mineral element intake. ‘Hasandede’ grape variety grafted on 1103 P American rootstock were grown in environments containing control, 10% and 25% calcium oxide (CaO) and applied with SL (control, 1, 3 and 5 μM) in different doses. Total phenolic compounds were found statistically much higher especially in the majority of plants applied with 3 μM and 5 μM SL is considered to be the effect of SLs in stress prevention. It was concluded that mineral compounds intakes generally responded positively to SL applications. These results are considered to prove the effects of SLs on plant nutrition physiology and stress tolerance.


Akiyama K, Matsuzaki K, Hayashi H (2005). Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature 435:824-827.

Andersen C (2003). Source-sink balance and carbon allocation below ground in plants exposed to ozone. New Phytologist 157:213-228.

Arnous A, Makris DP, Kefalas P (2001). Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. Journal of Agricultural and Food Chemistry 49(12):5736-5742.

Bavaresco L, Poni S (2003). Effect of calcareous soil on photosynthesis rate, mineral nutrition, and source-sink ration of table grape. Journal of Plant Nutrition 26:2123-2135.

Brady NC (1990). The nature and properties of soils. Macmillan Publishing Company (10th ed), New York, USA.

Christmann A, Moes D, Himmelbach A, Yang Y, Tang Y, Grill E (2006). Integration of abscisic acid signalling into plant responses. Plant Biology 8:314-325.

Cook CE, Whichard LP, Turner B, Wall ME, Egley GH (1966). Germination of witchweed (Striga lutea Lour.): Isolation and properties of a potent stimulant. Science 154:1189-1190.

Dai GH, Andary C, Mondolot L, Boubals D (1995). Involment of phenolic compounds in the resistance of grapevine callus to downy mildew (Plasmopara viticola). European Journal of Plant Pathology 101:541-547.

Dierck R, Dhooghe E, Huylenbroeck JV, De Riek J, De Keyser E, Van Der Straeten D (2016). Response to strigolactone treatment in chrysanthemum axillary buds is influenced by auxin transport inhibition and sucrose availability. Acta Physiologia Plantarum 38:271.

Einav MG, Lekkala, SP, Resnick N, Wininger S, Bhattacharya C, Lemcoff JH, … Koltai H (2010). Strigolactones are positive regulators of light-arvesting genes in tomato. Journal of Experimental Botany 61(11):3129-3136.

Evelin H, Kapoor R, Giri B (2009). Arbuscular mycorrhizal fungi in alleviation of salt stress: A review. Annual Botany 104:1263-1280.

Fageria NK (2009). The use of nutrients in crop plants. New York: CRC Pres, Boca Raton, Florida, USA.

Ferrero M, Pagliarani C, Novak O, Ferrandino A, Cardinale F, Visentin I, Schubert A (2018). Exogenous strigolactone interacts with abscisic acid mediated accumulation of anthocyanins in grapevine berries. Journal of Experimental Botany 69 (9):2391-2402.

Foo E, Reid JB (2012). Strigolactones: New physiological roles for an ancient signal. Journal of Plant Growth Regulation 32(2):429-442.

Foo E (2013). Auxin influences strigolactones in pea mycorrhizal symbiosis. Journal of Plant Physiology 170:523-528.

Foth HD (1984). Fundamentals of soil science. John Wiley and Sons (7th ed), New York.

Gardiner DT, Miller RW (2008). Soils in our environment. Pearson/Prentice Hall (11th ed), New Jersey, Upper Saddle Hill, USA.

German JB, Walzem RL (2000). The health benefits of wine. Annual Review of Nutrition 20(1):561-593.

Gök Ozel Ş (2018). The function of antioxidant enzymes in increasing tolerance of sand plant against salt stress by strigolactone application. MSc Thesis, Tekirdağ Namık Kemal Univ, Turkey.

Ha CV, Leyva Gonzales MA, Osakabe Y, Tran UT, Nishiyama R, Watanabe Y, … Tran LS (2014). Positive regulatory role of strigolactone in plant responses to drought and salt stress. Proceeding National Academy of Science U.S.A 14 (111):851-856.

Hirayama T, Shinozaki K (2010). Research on plant abiotic stress responses in the post-genome era: Past, present and future. The Plant Journal 61:1041-1052.

Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. Circular. California Agricultural Experiment Station 347.

Issah H (2021). The effects of strigolactones on callus culture and phenolic compound production of caper (Capparis spinosa L.). MSc Thesis, Süleyman Demirel Univ, Turkey.

Kapulnik Y, Delaux PM, Resnick N, Mayzlish Gati E, Wininger S, Bhattacharya C, … Koltai H (2011). Strigolactones affect lateral root formation and root-hair elongation in Arabidopsis. Planta 233:209-216.

Kausar F, Shahbaz M (2017). Influence of strigolactone (GR24) as a seed treatment on growth, gas exchange and chlorophyll fluorescence of wheat under saline conditions. International Journal of Agriculture & Biology 19(2):321-327.

Kiselev KV, Dubrovina AS, Veselova MV, Bulgakov VP, Fedoreyev SA, Zhuravlev YN (2007). The rol-B gene-induced over production of resveratrol in Vitis amurensis transformed cells. Journal of Biotechnology 128:681.

Kohlen W, Charnikhova T, Qing L, Bours R, Domagalska M, Beguerie S, … Ruyter Spira C (2011). Strigolactones are transported through the xylem and play a key role in shoot architectural response to phosphate deficiency in non-AM host Arabidopsis thaliana. Plant Physiology 155:974-987.

Lattanzio V, Cardinali A, Ruta C, Morone Fortunato I, Lattanzio VMT, Linsalata V, Cicco N (2009). Relationship of secondary metabolism to growth in Oregano (Origanum Vulgare L.) shoot cultures under nutritional stress. Environmental and Experimental Botany 65:54-62.

Liu J, Mehdi S, Topping J, Friml J, Lindsey K (2013). Interaction of PLS and PIN and hormonal crosstalk in arabidopsis root development. Frontiers in Plant Science 4(75):1-8.

Lopez Bucio J, Hernandez Abreu E, Sanchez Calderon L, Nieto Jacobo MF, Simpson J, Herrera Estrella L (2002). Phosphate availability alters architecture and causes changes in hormone sensitivity in the arabidopsis root system. Plant Physiology 129:244-256.

Lopez Raez JA, Kohlen W, Charnikhova T, Mulder P, Undas AK, Sergeant MJ, … Bouwmeester H (2010). Does abscisic acid affect strigolactone biosynthesis? New Phytologist 187:343-354.

Mahajan S, Tuteja N (2005). Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics 444(2):139-158.

Martin AC, Del Pozo JC, Iglesias J, Rubio V, Solano R, De La Pena A, Leyva A, Paz Ares J (2000). Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. Plant Journal 24:559-567.

Matusova R, Rani K, Verstappen FWA, Franssen MCR, Beale MH, Bouwmeester HJ (2005). The strigolactone germination stimulants of the plant-parasitic Striga and Orobanche spp. are derived from the carotenoid pathway. Plant Physiology 139:920-934.

McCauley A, Jones C, Jacobsen J (2009). Nutrient Management. Nutrient Management Module 9. Montana State University Extension Service. Publication 4449(9):1-16.

Middleton E, Kandaswami C, Theoharides TC (2000). The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews 52(4):673-751.

Min Z, Li R, Che L, Zhang Y, Li Z, Liu M, Ju Y, Fang Y (2019). Alleviation of drought stress in grapevine by foliar-applied strigolactones. Plant Physiology and Biochemistry 135:99-110.

Onay E (2019). Effect of strigolactone on enzyme system of ascorbate glutathione cycle in salt stress tolerant and sensitive wheat varieties. MSc Thesis, Tekirdağ Namık Kemal Univ, Turkey.

Ozcan ME, Guleç M, Ozerol E, Polat R, Akyol O (2004). Antioxidant enzyme activities and oxidative stress in affective disordes. International Clinical Psychopharmacology 19:89-95.

Perez Torres CA, Lopez Bucio J, Cruz Ramirez A, Ibarra Laclette E, Dharmasiri S, Estelle M, Herrera Estrella L (2008). Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor. Plant Cell 20(12):3258-3272.

Pezzuto JM (2008). Grapes and human health: A perspective. Journal of Agricultural and Food Chemistry 56(16):6777-6784.

Plaster EJ (1992). Soil Science and Management. Delmar Publishers Inc (2nd ed), Albany, New York, USA.

Raghavendra AS, Gonugunta VK, Christmann A, Grill E (2010). ABA perception and signaling. Trends in Plant Science 15:395-401.

Ren C, Guo Y, Kong J, Lecourieux F, Dai Z, Li S, Liang Z (2020). Knockout of Vvccd8 gene in grapevine affects shoot branching. BMC Plant Biology 20:47.

Roumeliotis E, Kloosterman B, Oortwijn M, Kohlen W, Bouwmeester HJ, Visser, RGF, Bachem CW (2012). The effects of auxin and strigolactones on tuber initiation and stolon architecture in potato. Journal of Experimental Botany 63:4539-4547.

Salama A, Wareing PF (1979). Effects of mineral nutrition on endogenous cytokinins in plants of sunflower (Helianthus annuus L.). Journal of Experimental Botany 30:971-981.

Sedaghat M, Tahmasebi Sarvestani Z, Emam Y, Mokhtassi Bidgoli A (2017). Physiological and antioxidant responses of winter wheat cultivars to strigolactone and salicylic acid in drought. Plant Physiology and Biochemistry 119:59-69.

Shalau J (2010). Laboratories conducting soil, plant, feed or water testing. Publication AZ1111, College of Agriculture and Life Science, University of Arizona.

Shinohara N, Taylor C, Leyser O (2013). Strigolactone can promote or inhibit shoot branching by triggering rapid depletion of the auxin efflux protein PIN1 from the plasma membrane. PLoS Biology 11:1-14.

Singleton VL, Rossi JR (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid. American Journal of Enology and Viticulture 16:144-158.

Torres Vera R, Garcia, JM, Pozo M, Lopez Raez JA (2014). Do strigolactones contribute to plant defence? Molecular Plant Pathology 15 (2):211-216.

Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda Kamiya N, … Yamaguchi S (2008). Inhibition of shoot branching by new terpenoid plant hormones. Nature 455:195-200.

White RE (2006). Principles and Practice of Soil Science: The Soil as a Natural Resource. Wiley-Blackwell Scientific Publication (4th ed), London, United Kingdom.

Wilkinson S, Clephan AL, Davies WJ (2001). Rapid low temperature induced stomatal closure occurs in cold-tolerant Commelina communis leaves but not in cold-sensitive tobacco leaves, via a mechanism that involves apoplastic calcium but not abscisic acid. Plant Physiology 126(4):1566-1578.

Yoneyama K, Xie X, Kusumoto D, Sekimoto H, Sugimoto Y, Takeuchi Y, Yoneyama K (2007). Nitrogen deficiency as well as phosphorus deficiency in sorghum promotes the production and exudation of 5-deoxystrigol, the host recognition signal for arbuscular mycorrhizal fungi and root parasites. Planta 227(1):125-132.

Yoshida S, Kameoka H, Tempo M, Akiyama K, Umehara M, Yamaguchi S, Hayashi H, Kyozuka J, Shirasu K (2012). The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis. New Phytologist 196:1208-1216.



How to Cite

ÇETİN, E. S., & KOÇ, B. (2022). The effects of strigolactones on some biochemical traits in calcified media on grapevine. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12816.



Research Articles
DOI: 10.15835/nbha50312816